Amino acid dipepetide frequency for Bacillus phage Basilisk

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.818AlaAla: 4.818 ± 0.651
0.756AlaCys: 0.756 ± 0.188
3.464AlaAsp: 3.464 ± 0.504
4.499AlaGlu: 4.499 ± 0.643
2.349AlaPhe: 2.349 ± 0.31
4.3AlaGly: 4.3 ± 0.51
0.597AlaHis: 0.597 ± 0.155
3.623AlaIle: 3.623 ± 0.457
5.495AlaLys: 5.495 ± 0.538
4.22AlaLeu: 4.22 ± 0.452
1.354AlaMet: 1.354 ± 0.289
3.623AlaAsn: 3.623 ± 0.565
1.115AlaPro: 1.115 ± 0.209
1.911AlaGln: 1.911 ± 0.384
1.911AlaArg: 1.911 ± 0.318
2.508AlaSer: 2.508 ± 0.391
3.504AlaThr: 3.504 ± 0.624
4.021AlaVal: 4.021 ± 0.401
0.876AlaTrp: 0.876 ± 0.201
2.588AlaTyr: 2.588 ± 0.362
0.0AlaXaa: 0.0 ± 0.0
Cys
0.279CysAla: 0.279 ± 0.108
0.119CysCys: 0.119 ± 0.071
0.916CysAsp: 0.916 ± 0.242
0.637CysGlu: 0.637 ± 0.175
0.438CysPhe: 0.438 ± 0.141
0.279CysGly: 0.279 ± 0.111
0.199CysHis: 0.199 ± 0.092
0.756CysIle: 0.756 ± 0.224
0.836CysLys: 0.836 ± 0.185
0.518CysLeu: 0.518 ± 0.148
0.398CysMet: 0.398 ± 0.127
0.478CysAsn: 0.478 ± 0.159
0.557CysPro: 0.557 ± 0.163
0.199CysGln: 0.199 ± 0.087
0.358CysArg: 0.358 ± 0.121
0.438CysSer: 0.438 ± 0.15
0.438CysThr: 0.438 ± 0.146
0.518CysVal: 0.518 ± 0.158
0.08CysTrp: 0.08 ± 0.055
0.319CysTyr: 0.319 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
3.106AspAla: 3.106 ± 0.392
0.518AspCys: 0.518 ± 0.173
3.464AspAsp: 3.464 ± 0.383
4.937AspGlu: 4.937 ± 0.491
2.946AspPhe: 2.946 ± 0.463
3.703AspGly: 3.703 ± 0.39
1.194AspHis: 1.194 ± 0.218
5.256AspIle: 5.256 ± 0.477
6.012AspLys: 6.012 ± 0.523
4.897AspLeu: 4.897 ± 0.491
1.553AspMet: 1.553 ± 0.224
4.021AspAsn: 4.021 ± 0.463
1.871AspPro: 1.871 ± 0.284
2.031AspGln: 2.031 ± 0.331
3.026AspArg: 3.026 ± 0.468
3.106AspSer: 3.106 ± 0.363
3.344AspThr: 3.344 ± 0.357
4.061AspVal: 4.061 ± 0.356
0.995AspTrp: 0.995 ± 0.181
3.424AspTyr: 3.424 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
4.3GluAla: 4.3 ± 0.512
0.597GluCys: 0.597 ± 0.177
3.782GluAsp: 3.782 ± 0.444
6.41GluGlu: 6.41 ± 0.746
3.066GluPhe: 3.066 ± 0.378
4.3GluGly: 4.3 ± 0.394
0.916GluHis: 0.916 ± 0.191
5.813GluIle: 5.813 ± 0.517
6.848GluLys: 6.848 ± 0.537
6.808GluLeu: 6.808 ± 0.69
2.827GluMet: 2.827 ± 0.317
4.22GluAsn: 4.22 ± 0.409
1.752GluPro: 1.752 ± 0.254
3.225GluGln: 3.225 ± 0.39
3.265GluArg: 3.265 ± 0.465
4.26GluSer: 4.26 ± 0.421
4.579GluThr: 4.579 ± 0.464
5.017GluVal: 5.017 ± 0.448
1.075GluTrp: 1.075 ± 0.225
3.185GluTyr: 3.185 ± 0.429
0.0GluXaa: 0.0 ± 0.0
Phe
2.588PheAla: 2.588 ± 0.286
0.478PheCys: 0.478 ± 0.117
3.544PheAsp: 3.544 ± 0.406
3.782PheGlu: 3.782 ± 0.411
1.672PhePhe: 1.672 ± 0.274
2.747PheGly: 2.747 ± 0.413
0.597PheHis: 0.597 ± 0.155
3.066PheIle: 3.066 ± 0.377
3.623PheLys: 3.623 ± 0.345
3.344PheLeu: 3.344 ± 0.452
1.194PheMet: 1.194 ± 0.218
2.867PheAsn: 2.867 ± 0.337
1.155PhePro: 1.155 ± 0.201
1.155PheGln: 1.155 ± 0.199
1.513PheArg: 1.513 ± 0.237
2.429PheSer: 2.429 ± 0.358
3.424PheThr: 3.424 ± 0.403
2.469PheVal: 2.469 ± 0.311
0.637PheTrp: 0.637 ± 0.185
1.792PheTyr: 1.792 ± 0.313
0.0PheXaa: 0.0 ± 0.0
Gly
2.946GlyAla: 2.946 ± 0.537
0.756GlyCys: 0.756 ± 0.189
2.668GlyAsp: 2.668 ± 0.344
4.26GlyGlu: 4.26 ± 0.379
2.946GlyPhe: 2.946 ± 0.357
4.897GlyGly: 4.897 ± 0.725
1.234GlyHis: 1.234 ± 0.226
4.141GlyIle: 4.141 ± 0.354
5.176GlyLys: 5.176 ± 0.414
5.375GlyLeu: 5.375 ± 0.407
2.23GlyMet: 2.23 ± 0.363
3.663GlyAsn: 3.663 ± 0.49
0.08GlyPro: 0.08 ± 0.052
2.668GlyGln: 2.668 ± 0.8
2.867GlyArg: 2.867 ± 0.325
3.822GlySer: 3.822 ± 0.471
4.22GlyThr: 4.22 ± 0.713
4.738GlyVal: 4.738 ± 0.55
0.995GlyTrp: 0.995 ± 0.187
3.185GlyTyr: 3.185 ± 0.35
0.0GlyXaa: 0.0 ± 0.0
His
0.796HisAla: 0.796 ± 0.161
0.119HisCys: 0.119 ± 0.077
1.115HisAsp: 1.115 ± 0.213
0.836HisGlu: 0.836 ± 0.162
0.956HisPhe: 0.956 ± 0.196
1.075HisGly: 1.075 ± 0.203
0.199HisHis: 0.199 ± 0.082
1.274HisIle: 1.274 ± 0.213
1.473HisLys: 1.473 ± 0.267
1.672HisLeu: 1.672 ± 0.252
0.478HisMet: 0.478 ± 0.15
1.394HisAsn: 1.394 ± 0.27
0.478HisPro: 0.478 ± 0.134
0.518HisGln: 0.518 ± 0.136
0.398HisArg: 0.398 ± 0.129
0.836HisSer: 0.836 ± 0.148
1.274HisThr: 1.274 ± 0.37
0.995HisVal: 0.995 ± 0.233
0.239HisTrp: 0.239 ± 0.098
0.956HisTyr: 0.956 ± 0.266
0.0HisXaa: 0.0 ± 0.0
Ile
3.982IleAla: 3.982 ± 0.377
0.637IleCys: 0.637 ± 0.176
6.211IleAsp: 6.211 ± 0.543
5.614IleGlu: 5.614 ± 0.456
2.469IlePhe: 2.469 ± 0.331
4.778IleGly: 4.778 ± 0.399
1.433IleHis: 1.433 ± 0.215
4.141IleIle: 4.141 ± 0.506
7.326IleLys: 7.326 ± 0.58
4.101IleLeu: 4.101 ± 0.36
1.951IleMet: 1.951 ± 0.279
4.26IleAsn: 4.26 ± 0.473
1.911IlePro: 1.911 ± 0.264
2.23IleGln: 2.23 ± 0.297
2.508IleArg: 2.508 ± 0.329
3.902IleSer: 3.902 ± 0.465
4.937IleThr: 4.937 ± 0.514
4.778IleVal: 4.778 ± 0.393
0.438IleTrp: 0.438 ± 0.171
2.747IleTyr: 2.747 ± 0.363
0.0IleXaa: 0.0 ± 0.0
Lys
4.897LysAla: 4.897 ± 0.508
0.916LysCys: 0.916 ± 0.206
5.773LysAsp: 5.773 ± 0.471
8.441LysGlu: 8.441 ± 0.703
3.663LysPhe: 3.663 ± 0.311
4.857LysGly: 4.857 ± 0.368
0.995LysHis: 0.995 ± 0.204
6.37LysIle: 6.37 ± 0.448
7.007LysLys: 7.007 ± 0.757
7.326LysLeu: 7.326 ± 0.506
3.185LysMet: 3.185 ± 0.355
4.3LysAsn: 4.3 ± 0.392
2.787LysPro: 2.787 ± 0.437
3.026LysGln: 3.026 ± 0.373
3.544LysArg: 3.544 ± 0.365
4.459LysSer: 4.459 ± 0.403
4.499LysThr: 4.499 ± 0.459
7.366LysVal: 7.366 ± 0.571
0.956LysTrp: 0.956 ± 0.188
4.539LysTyr: 4.539 ± 0.42
0.0LysXaa: 0.0 ± 0.0
Leu
5.017LeuAla: 5.017 ± 0.496
0.597LeuCys: 0.597 ± 0.156
5.176LeuAsp: 5.176 ± 0.396
6.45LeuGlu: 6.45 ± 0.553
3.026LeuPhe: 3.026 ± 0.4
4.419LeuGly: 4.419 ± 0.455
1.672LeuHis: 1.672 ± 0.236
4.698LeuIle: 4.698 ± 0.416
8.043LeuLys: 8.043 ± 0.555
5.017LeuLeu: 5.017 ± 0.511
2.15LeuMet: 2.15 ± 0.32
5.694LeuAsn: 5.694 ± 0.511
2.588LeuPro: 2.588 ± 0.342
2.907LeuGln: 2.907 ± 0.415
3.384LeuArg: 3.384 ± 0.352
4.021LeuSer: 4.021 ± 0.372
5.733LeuThr: 5.733 ± 0.44
3.942LeuVal: 3.942 ± 0.463
1.115LeuTrp: 1.115 ± 0.214
2.548LeuTyr: 2.548 ± 0.366
0.0LeuXaa: 0.0 ± 0.0
Met
1.632MetAla: 1.632 ± 0.297
0.239MetCys: 0.239 ± 0.1
1.593MetAsp: 1.593 ± 0.234
2.07MetGlu: 2.07 ± 0.278
1.274MetPhe: 1.274 ± 0.24
1.513MetGly: 1.513 ± 0.309
0.478MetHis: 0.478 ± 0.14
2.508MetIle: 2.508 ± 0.29
3.265MetLys: 3.265 ± 0.328
2.23MetLeu: 2.23 ± 0.27
0.916MetMet: 0.916 ± 0.198
1.672MetAsn: 1.672 ± 0.377
0.637MetPro: 0.637 ± 0.19
1.075MetGln: 1.075 ± 0.175
1.274MetArg: 1.274 ± 0.239
2.19MetSer: 2.19 ± 0.278
1.672MetThr: 1.672 ± 0.218
2.469MetVal: 2.469 ± 0.305
0.239MetTrp: 0.239 ± 0.089
1.513MetTyr: 1.513 ± 0.223
0.0MetXaa: 0.0 ± 0.0
Asn
2.707AsnAla: 2.707 ± 0.348
0.358AsnCys: 0.358 ± 0.157
3.782AsnAsp: 3.782 ± 0.344
4.3AsnGlu: 4.3 ± 0.43
2.309AsnPhe: 2.309 ± 0.33
4.459AsnGly: 4.459 ± 0.53
0.995AsnHis: 0.995 ± 0.221
4.3AsnIle: 4.3 ± 0.355
5.654AsnLys: 5.654 ± 0.579
5.415AsnLeu: 5.415 ± 0.466
2.389AsnMet: 2.389 ± 0.259
3.982AsnAsn: 3.982 ± 0.355
2.031AsnPro: 2.031 ± 0.397
2.389AsnGln: 2.389 ± 0.465
2.469AsnArg: 2.469 ± 0.391
3.623AsnSer: 3.623 ± 0.507
2.827AsnThr: 2.827 ± 0.437
3.663AsnVal: 3.663 ± 0.435
0.756AsnTrp: 0.756 ± 0.186
2.429AsnTyr: 2.429 ± 0.363
0.0AsnXaa: 0.0 ± 0.0
Pro
1.672ProAla: 1.672 ± 0.228
0.199ProCys: 0.199 ± 0.094
1.553ProAsp: 1.553 ± 0.326
2.309ProGlu: 2.309 ± 0.304
1.712ProPhe: 1.712 ± 0.363
0.0ProGly: 0.0 ± 0.0
0.358ProHis: 0.358 ± 0.11
2.15ProIle: 2.15 ± 0.299
2.548ProLys: 2.548 ± 0.321
2.031ProLeu: 2.031 ± 0.298
1.035ProMet: 1.035 ± 0.222
1.951ProAsn: 1.951 ± 0.385
0.358ProPro: 0.358 ± 0.124
1.075ProGln: 1.075 ± 0.24
0.518ProArg: 0.518 ± 0.134
1.752ProSer: 1.752 ± 0.276
1.832ProThr: 1.832 ± 0.314
1.871ProVal: 1.871 ± 0.225
0.199ProTrp: 0.199 ± 0.082
1.274ProTyr: 1.274 ± 0.247
0.0ProXaa: 0.0 ± 0.0
Gln
1.991GlnAla: 1.991 ± 0.42
0.239GlnCys: 0.239 ± 0.085
1.394GlnAsp: 1.394 ± 0.231
2.827GlnGlu: 2.827 ± 0.45
1.155GlnPhe: 1.155 ± 0.249
2.389GlnGly: 2.389 ± 0.488
0.478GlnHis: 0.478 ± 0.171
2.469GlnIle: 2.469 ± 0.333
2.787GlnLys: 2.787 ± 0.34
3.623GlnLeu: 3.623 ± 0.405
1.274GlnMet: 1.274 ± 0.223
2.349GlnAsn: 2.349 ± 0.337
1.035GlnPro: 1.035 ± 0.381
2.946GlnGln: 2.946 ± 1.556
1.632GlnArg: 1.632 ± 0.29
1.632GlnSer: 1.632 ± 0.214
1.871GlnThr: 1.871 ± 0.399
2.11GlnVal: 2.11 ± 0.267
0.438GlnTrp: 0.438 ± 0.125
1.354GlnTyr: 1.354 ± 0.234
0.0GlnXaa: 0.0 ± 0.0
Arg
2.309ArgAla: 2.309 ± 0.339
0.239ArgCys: 0.239 ± 0.101
2.588ArgAsp: 2.588 ± 0.305
2.787ArgGlu: 2.787 ± 0.364
2.11ArgPhe: 2.11 ± 0.302
2.508ArgGly: 2.508 ± 0.37
0.876ArgHis: 0.876 ± 0.214
2.747ArgIle: 2.747 ± 0.363
3.384ArgLys: 3.384 ± 0.376
2.946ArgLeu: 2.946 ± 0.375
1.354ArgMet: 1.354 ± 0.258
2.309ArgAsn: 2.309 ± 0.284
1.155ArgPro: 1.155 ± 0.256
1.354ArgGln: 1.354 ± 0.222
1.433ArgArg: 1.433 ± 0.24
1.752ArgSer: 1.752 ± 0.202
2.23ArgThr: 2.23 ± 0.246
2.429ArgVal: 2.429 ± 0.326
0.398ArgTrp: 0.398 ± 0.102
1.792ArgTyr: 1.792 ± 0.357
0.0ArgXaa: 0.0 ± 0.0
Ser
3.623SerAla: 3.623 ± 0.579
0.557SerCys: 0.557 ± 0.136
2.946SerAsp: 2.946 ± 0.374
3.424SerGlu: 3.424 ± 0.367
3.504SerPhe: 3.504 ± 0.407
4.778SerGly: 4.778 ± 0.532
1.035SerHis: 1.035 ± 0.215
3.782SerIle: 3.782 ± 0.431
4.141SerLys: 4.141 ± 0.318
4.778SerLeu: 4.778 ± 0.385
1.593SerMet: 1.593 ± 0.351
2.867SerAsn: 2.867 ± 0.386
1.473SerPro: 1.473 ± 0.286
1.911SerGln: 1.911 ± 0.323
1.274SerArg: 1.274 ± 0.262
3.464SerSer: 3.464 ± 0.458
2.946SerThr: 2.946 ± 0.33
2.747SerVal: 2.747 ± 0.334
0.836SerTrp: 0.836 ± 0.266
2.668SerTyr: 2.668 ± 0.278
0.0SerXaa: 0.0 ± 0.0
Thr
4.181ThrAla: 4.181 ± 0.793
0.398ThrCys: 0.398 ± 0.145
3.982ThrAsp: 3.982 ± 0.44
3.703ThrGlu: 3.703 ± 0.39
2.707ThrPhe: 2.707 ± 0.319
4.778ThrGly: 4.778 ± 0.565
1.354ThrHis: 1.354 ± 0.295
5.256ThrIle: 5.256 ± 0.484
4.778ThrLys: 4.778 ± 0.414
4.738ThrLeu: 4.738 ± 0.551
1.354ThrMet: 1.354 ± 0.259
3.504ThrAsn: 3.504 ± 0.399
2.19ThrPro: 2.19 ± 0.317
1.632ThrGln: 1.632 ± 0.287
2.15ThrArg: 2.15 ± 0.253
3.344ThrSer: 3.344 ± 0.542
4.579ThrThr: 4.579 ± 0.588
4.818ThrVal: 4.818 ± 0.464
0.319ThrTrp: 0.319 ± 0.1
2.548ThrTyr: 2.548 ± 0.31
0.0ThrXaa: 0.0 ± 0.0
Val
3.623ValAla: 3.623 ± 0.464
0.518ValCys: 0.518 ± 0.136
4.658ValAsp: 4.658 ± 0.472
4.539ValGlu: 4.539 ± 0.354
3.026ValPhe: 3.026 ± 0.339
3.623ValGly: 3.623 ± 0.357
1.314ValHis: 1.314 ± 0.275
4.141ValIle: 4.141 ± 0.44
6.53ValLys: 6.53 ± 0.514
4.579ValLeu: 4.579 ± 0.438
1.672ValMet: 1.672 ± 0.331
4.021ValAsn: 4.021 ± 0.434
2.269ValPro: 2.269 ± 0.278
2.349ValGln: 2.349 ± 0.346
2.867ValArg: 2.867 ± 0.338
3.623ValSer: 3.623 ± 0.297
4.818ValThr: 4.818 ± 0.562
4.658ValVal: 4.658 ± 0.489
0.956ValTrp: 0.956 ± 0.193
2.668ValTyr: 2.668 ± 0.387
0.0ValXaa: 0.0 ± 0.0
Trp
0.478TrpAla: 0.478 ± 0.191
0.119TrpCys: 0.119 ± 0.07
1.115TrpAsp: 1.115 ± 0.2
0.677TrpGlu: 0.677 ± 0.17
0.756TrpPhe: 0.756 ± 0.178
0.756TrpGly: 0.756 ± 0.192
0.358TrpHis: 0.358 ± 0.121
0.916TrpIle: 0.916 ± 0.223
0.717TrpLys: 0.717 ± 0.189
0.756TrpLeu: 0.756 ± 0.198
0.279TrpMet: 0.279 ± 0.124
0.916TrpAsn: 0.916 ± 0.213
0.04TrpPro: 0.04 ± 0.033
0.438TrpGln: 0.438 ± 0.155
0.358TrpArg: 0.358 ± 0.147
0.677TrpSer: 0.677 ± 0.239
0.836TrpThr: 0.836 ± 0.205
1.035TrpVal: 1.035 ± 0.191
0.08TrpTrp: 0.08 ± 0.057
0.717TrpTyr: 0.717 ± 0.185
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.588TyrAla: 2.588 ± 0.373
0.398TyrCys: 0.398 ± 0.128
3.743TyrAsp: 3.743 ± 0.42
3.743TyrGlu: 3.743 ± 0.383
1.991TyrPhe: 1.991 ± 0.305
2.707TyrGly: 2.707 ± 0.34
0.796TyrHis: 0.796 ± 0.18
2.946TyrIle: 2.946 ± 0.41
3.265TyrLys: 3.265 ± 0.365
3.822TyrLeu: 3.822 ± 0.44
1.234TyrMet: 1.234 ± 0.174
2.747TyrAsn: 2.747 ± 0.325
0.916TyrPro: 0.916 ± 0.187
0.956TyrGln: 0.956 ± 0.154
1.951TyrArg: 1.951 ± 0.302
2.508TyrSer: 2.508 ± 0.369
2.787TyrThr: 2.787 ± 0.405
2.747TyrVal: 2.747 ± 0.323
0.398TyrTrp: 0.398 ± 0.121
2.747TyrTyr: 2.747 ± 0.402
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 138 proteins (25117 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski