Phocoena phocoena papillomavirus 4
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2VIS2|F2VIS2_9PAPI Regulatory protein E2 OS=Phocoena phocoena papillomavirus 4 OX=706527 GN=E2 PE=3 SV=1
MM1 pKa = 8.15 DD2 pKa = 5.89 PPQGTDD8 pKa = 3.02 GDD10 pKa = 4.34 EE11 pKa = 4.89 GCSGWCFVDD20 pKa = 6.23 LEE22 pKa = 4.2 AQEE25 pKa = 5.81 DD26 pKa = 4.67 GVDD29 pKa = 5.04 DD30 pKa = 4.67 NDD32 pKa = 4.38 NDD34 pKa = 4.13 DD35 pKa = 3.92 EE36 pKa = 4.95 SVDD39 pKa = 3.71 EE40 pKa = 4.07 EE41 pKa = 5.05 DD42 pKa = 3.77 YY43 pKa = 11.51 AIVNLFDD50 pKa = 6.13 DD51 pKa = 4.43 EE52 pKa = 6.34 DD53 pKa = 4.01 VTQGNSLTVYY63 pKa = 9.31 EE64 pKa = 4.38 AQNRR68 pKa = 11.84 ADD70 pKa = 4.87 DD71 pKa = 3.73 EE72 pKa = 4.51 RR73 pKa = 11.84 AVQKK77 pKa = 10.6 LKK79 pKa = 10.9 RR80 pKa = 11.84 KK81 pKa = 9.15 LLQSPEE87 pKa = 4.44 LDD89 pKa = 3.15 LSPRR93 pKa = 11.84 LSAITIGKK101 pKa = 9.97 KK102 pKa = 9.39 SGKK105 pKa = 9.46 AKK107 pKa = 10.15 RR108 pKa = 11.84 RR109 pKa = 11.84 LFMPGQDD116 pKa = 2.89 SGYY119 pKa = 8.6 GQSVEE124 pKa = 4.32 ANVEE128 pKa = 4.03 EE129 pKa = 5.32 SVLQTQVSLNFNGEE143 pKa = 3.96 DD144 pKa = 3.4 SGGGGGAPCTVSGAQEE160 pKa = 4.14 TPEE163 pKa = 4.56 TMIQLLKK170 pKa = 10.18 QANPQAAMMGLFKK183 pKa = 10.26 KK184 pKa = 9.36 TMGISFTDD192 pKa = 3.91 LSRR195 pKa = 11.84 TFQSDD200 pKa = 3.17 KK201 pKa = 10.33 TPCVDD206 pKa = 3.14 WVAVCFGVPLSLIDD220 pKa = 3.58 ACTEE224 pKa = 3.77 LLQPHH229 pKa = 6.63 CEE231 pKa = 4.06 FGNVTTTPCCTGYY244 pKa = 11.2 LLLLLARR251 pKa = 11.84 FQVAKK256 pKa = 10.66 CRR258 pKa = 11.84 VTVKK262 pKa = 10.69 KK263 pKa = 10.85 LLGNTLHH270 pKa = 7.23 VPEE273 pKa = 4.24 QQILTEE279 pKa = 4.19 PPKK282 pKa = 10.33 IRR284 pKa = 11.84 CPAAAIYY291 pKa = 8.97 WYY293 pKa = 10.25 KK294 pKa = 10.89 KK295 pKa = 10.39 GITGVAKK302 pKa = 9.33 TWGPLPEE309 pKa = 4.34 WLAKK313 pKa = 8.54 MVNVSSNLADD323 pKa = 3.25 AAVFNLSDD331 pKa = 4.35 MIQWAYY337 pKa = 11.19 DD338 pKa = 3.45 NNKK341 pKa = 8.75 TEE343 pKa = 4.93 DD344 pKa = 3.27 ADD346 pKa = 3.39 IAYY349 pKa = 9.74 GYY351 pKa = 10.47 AQLADD356 pKa = 3.43 TDD358 pKa = 4.25 RR359 pKa = 11.84 NAEE362 pKa = 4.12 AFLKK366 pKa = 10.95 SNSQAKK372 pKa = 8.57 YY373 pKa = 10.44 VKK375 pKa = 10.13 DD376 pKa = 3.53 CGVMVRR382 pKa = 11.84 LYY384 pKa = 10.73 KK385 pKa = 10.13 KK386 pKa = 10.81 AEE388 pKa = 3.91 MQRR391 pKa = 11.84 TSMKK395 pKa = 10.27 DD396 pKa = 2.91 WIKK399 pKa = 10.62 RR400 pKa = 11.84 RR401 pKa = 11.84 CEE403 pKa = 3.93 EE404 pKa = 4.07 VDD406 pKa = 3.84 GDD408 pKa = 4.32 GDD410 pKa = 3.39 WRR412 pKa = 11.84 PIVSFLKK419 pKa = 10.72 FQGIDD424 pKa = 3.06 MAVFISVLKK433 pKa = 10.77 NFLKK437 pKa = 10.92 GIPKK441 pKa = 9.61 QNCIVICGPPNTGKK455 pKa = 10.6 SLFSVSLIQFLSGKK469 pKa = 10.0 VISFVNSKK477 pKa = 8.57 SHH479 pKa = 7.02 FWLQPLGEE487 pKa = 4.64 CKK489 pKa = 10.17 VALLDD494 pKa = 5.24 DD495 pKa = 4.1 ATGPAWDD502 pKa = 4.22 YY503 pKa = 11.58 FDD505 pKa = 3.62 TFLRR509 pKa = 11.84 NLVDD513 pKa = 4.08 GNPISLDD520 pKa = 3.89 CKK522 pKa = 10.46 HH523 pKa = 6.62 KK524 pKa = 11.09 APTQVKK530 pKa = 9.21 CPPLIITSNIDD541 pKa = 2.95 VTASDD546 pKa = 3.02 RR547 pKa = 11.84 WKK549 pKa = 10.49 YY550 pKa = 10.0 LHH552 pKa = 7.01 SRR554 pKa = 11.84 LKK556 pKa = 11.04 LFTFSNPCPLTEE568 pKa = 4.04 QGEE571 pKa = 4.37 PAFNLTPVNWKK582 pKa = 10.37 CFFKK586 pKa = 10.76 RR587 pKa = 11.84 LKK589 pKa = 9.68 TRR591 pKa = 11.84 LGLADD596 pKa = 5.7 DD597 pKa = 4.35 EE598 pKa = 5.48 GEE600 pKa = 4.18 DD601 pKa = 4.1 HH602 pKa = 7.37 GEE604 pKa = 3.89 SQQPLKK610 pKa = 10.17 CTARR614 pKa = 11.84 GTDD617 pKa = 3.33 GFVV620 pKa = 2.77
Molecular weight: 68.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.974
IPC2_protein 5.029
IPC_protein 4.991
Toseland 4.863
ProMoST 5.118
Dawson 4.965
Bjellqvist 5.105
Wikipedia 4.863
Rodwell 4.863
Grimsley 4.774
Solomon 4.965
Lehninger 4.927
Nozaki 5.08
DTASelect 5.283
Thurlkill 4.876
EMBOSS 4.889
Sillero 5.143
Patrickios 4.024
IPC_peptide 4.978
IPC2_peptide 5.13
IPC2.peptide.svr19 5.154
Protein with the highest isoelectric point:
>tr|F2VIS3|F2VIS3_9PAPI E4 protein (Fragment) OS=Phocoena phocoena papillomavirus 4 OX=706527 GN=E4 PE=4 SV=1
LL1 pKa = 6.64 TLNLLLAQPIPRR13 pKa = 11.84 PPRR16 pKa = 11.84 VPVPRR21 pKa = 11.84 SPYY24 pKa = 10.5 LDD26 pKa = 3.72 LLGHH30 pKa = 6.9 IPRR33 pKa = 11.84 TPPQQHH39 pKa = 6.67 APPTCPRR46 pKa = 11.84 LQRR49 pKa = 11.84 RR50 pKa = 11.84 GNPGRR55 pKa = 11.84 QPLPRR60 pKa = 11.84 ADD62 pKa = 3.81 KK63 pKa = 11.0 DD64 pKa = 4.06 PIRR67 pKa = 11.84 KK68 pKa = 9.29 RR69 pKa = 11.84 LWDD72 pKa = 3.77 SDD74 pKa = 3.6 DD75 pKa = 4.28 SEE77 pKa = 4.57 NKK79 pKa = 10.17 EE80 pKa = 4.47 NIPPLQQQGPYY91 pKa = 9.22 TVDD94 pKa = 4.36 FPPIGPLDD102 pKa = 3.9 PPVTVTLTVTFQQ114 pKa = 2.95
Molecular weight: 12.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.187
IPC2_protein 9.107
IPC_protein 9.472
Toseland 9.897
ProMoST 9.648
Dawson 10.116
Bjellqvist 9.838
Wikipedia 10.306
Rodwell 10.277
Grimsley 10.189
Solomon 10.218
Lehninger 10.189
Nozaki 9.955
DTASelect 9.823
Thurlkill 9.97
EMBOSS 10.306
Sillero 10.043
Patrickios 10.058
IPC_peptide 10.204
IPC2_peptide 8.756
IPC2.peptide.svr19 8.065
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2281
114
620
380.2
42.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.612 ± 0.666
2.499 ± 0.604
6.357 ± 0.644
5.042 ± 0.414
3.902 ± 0.455
7.541 ± 0.841
1.929 ± 0.288
4.603 ± 0.551
5.48 ± 0.621
8.242 ± 0.664
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.666 ± 0.285
3.683 ± 0.287
7.321 ± 1.289
4.428 ± 0.382
5.305 ± 0.602
7.453 ± 0.667
7.716 ± 0.752
6.401 ± 0.235
1.622 ± 0.339
3.2 ± 0.58
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here