Helicobacter sp. 13S00401-1
Average proteome isoelectric point is 7.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1584 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A268TY85|A0A268TY85_9HELI Uncharacterized protein OS=Helicobacter sp. 13S00401-1 OX=1905758 GN=BKH43_06410 PE=4 SV=1
MM1 pKa = 7.58 CLAIPSKK8 pKa = 10.41 IISIDD13 pKa = 3.14 KK14 pKa = 10.07 DD15 pKa = 3.81 ANSVMLDD22 pKa = 3.17 TMGVQRR28 pKa = 11.84 SASLDD33 pKa = 3.5 LMEE36 pKa = 6.44 DD37 pKa = 3.46 EE38 pKa = 4.89 VNIGEE43 pKa = 4.3 YY44 pKa = 10.56 VLLHH48 pKa = 6.03 IGYY51 pKa = 9.58 VISKK55 pKa = 9.19 MDD57 pKa = 3.51 EE58 pKa = 3.83 KK59 pKa = 11.21 DD60 pKa = 3.33 ALEE63 pKa = 4.06 SLEE66 pKa = 4.51 LYY68 pKa = 9.55 KK69 pKa = 10.84 QIVASMEE76 pKa = 4.09 EE77 pKa = 4.16 EE78 pKa = 4.22 EE79 pKa = 4.4 EE80 pKa = 4.15
Molecular weight: 8.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.233
IPC2_protein 4.101
IPC_protein 3.999
Toseland 3.821
ProMoST 4.075
Dawson 3.961
Bjellqvist 4.177
Wikipedia 3.846
Rodwell 3.834
Grimsley 3.745
Solomon 3.948
Lehninger 3.897
Nozaki 4.075
DTASelect 4.215
Thurlkill 3.859
EMBOSS 3.859
Sillero 4.113
Patrickios 3.478
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 4.032
Protein with the highest isoelectric point:
>tr|A0A268U3B7|A0A268U3B7_9HELI Uncharacterized protein OS=Helicobacter sp. 13S00401-1 OX=1905758 GN=BKH43_02005 PE=3 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.34 RR14 pKa = 11.84 THH16 pKa = 5.91 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MSTKK25 pKa = 10.24 NGRR28 pKa = 11.84 KK29 pKa = 9.33 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.55 GRR39 pKa = 11.84 KK40 pKa = 8.81 RR41 pKa = 11.84 LSISS45 pKa = 2.96
Molecular weight: 5.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1584
0
1584
519509
37
5300
328.0
36.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.097 ± 0.069
0.968 ± 0.023
5.553 ± 0.053
5.887 ± 0.071
4.978 ± 0.056
6.182 ± 0.065
1.723 ± 0.022
7.971 ± 0.057
8.811 ± 0.076
10.983 ± 0.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.258 ± 0.032
5.332 ± 0.081
3.178 ± 0.033
2.643 ± 0.034
3.373 ± 0.038
7.732 ± 0.061
4.91 ± 0.049
6.06 ± 0.061
0.606 ± 0.02
3.755 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here