Mesorhizobium sp. WSM3876

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium; unclassified Mesorhizobium

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5672 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A3C3P5|A0A2A3C3P5_9RHIZ Uncharacterized protein OS=Mesorhizobium sp. WSM3876 OX=422277 GN=CK216_28080 PE=4 SV=1
MM1 pKa = 7.41ATTTISGAGGTTTSFSNTPQAQGDD25 pKa = 4.03VFNYY29 pKa = 10.18TEE31 pKa = 4.6DD32 pKa = 3.3NVVIVNAAQSIILLDD47 pKa = 3.64VMANDD52 pKa = 4.39LGGNAKK58 pKa = 8.36TLFSVDD64 pKa = 3.98DD65 pKa = 5.1GISASTATKK74 pKa = 10.0QYY76 pKa = 11.53APIDD80 pKa = 3.89LTTQDD85 pKa = 3.34VQATGISNWEE95 pKa = 4.24SIGGGVSIRR104 pKa = 11.84INNGKK109 pKa = 9.49VEE111 pKa = 4.22MDD113 pKa = 3.97LSQYY117 pKa = 10.84LLSHH121 pKa = 6.72GFTSLQALGAGDD133 pKa = 4.57NINEE137 pKa = 4.29TFTYY141 pKa = 10.08AIKK144 pKa = 10.66LGNGTLSWASVSVNIQGKK162 pKa = 9.66NDD164 pKa = 3.46GATITAAAAVDD175 pKa = 3.79NTVVEE180 pKa = 4.5AGGVANGTPGDD191 pKa = 4.01PSAHH195 pKa = 5.59GQLIISDD202 pKa = 3.31VDD204 pKa = 3.81LNEE207 pKa = 4.23NHH209 pKa = 6.04FQTPPSLQGTYY220 pKa = 9.3GTFTFDD226 pKa = 3.14ATSGAWTYY234 pKa = 11.9ALNQTLADD242 pKa = 4.61PLTQGQQVTDD252 pKa = 3.6MLQVKK257 pKa = 9.75SADD260 pKa = 3.19GTATYY265 pKa = 10.51NIVVNITGTNDD276 pKa = 3.24AAVVSHH282 pKa = 6.71DD283 pKa = 3.63VRR285 pKa = 11.84NLTEE289 pKa = 4.24GNAAADD295 pKa = 3.49ISTSGTLTISDD306 pKa = 4.02VDD308 pKa = 3.93SDD310 pKa = 3.88PHH312 pKa = 7.06FVAQAGTAGLYY323 pKa = 8.44GTFAIDD329 pKa = 5.21ADD331 pKa = 4.43GAWTYY336 pKa = 8.0TASSAHH342 pKa = 6.81DD343 pKa = 3.62EE344 pKa = 4.53FVAGTTYY351 pKa = 10.86TDD353 pKa = 3.24TFNVVSADD361 pKa = 3.65GTPTTVTINILGTNDD376 pKa = 3.45AAVLSSDD383 pKa = 3.73VKK385 pKa = 11.29NLTEE389 pKa = 3.96TNAAADD395 pKa = 3.47ISTSGTLL402 pKa = 3.33

Molecular weight: 41.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A3CGI6|A0A2A3CGI6_9RHIZ Uncharacterized protein OS=Mesorhizobium sp. WSM3876 OX=422277 GN=CK216_05975 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.11GGRR28 pKa = 11.84GVVAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5672

0

5672

1736559

26

2860

306.2

33.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.536 ± 0.041

0.847 ± 0.01

5.62 ± 0.024

5.674 ± 0.028

3.842 ± 0.021

8.542 ± 0.032

2.044 ± 0.015

5.35 ± 0.021

3.717 ± 0.027

9.949 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.503 ± 0.015

2.674 ± 0.017

5.041 ± 0.024

3.068 ± 0.018

6.934 ± 0.035

5.607 ± 0.023

5.08 ± 0.023

7.404 ± 0.025

1.33 ± 0.013

2.24 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski