Vibrio phage Va_90-11-287_p41_Ba35

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A513SRA6|A0A513SRA6_9CAUD Uncharacterized protein OS=Vibrio phage Va_90-11-287_p41_Ba35 OX=2565521 PE=4 SV=1
MM1 pKa = 7.81VPIRR5 pKa = 11.84DD6 pKa = 3.47VLLTHH11 pKa = 6.69EE12 pKa = 5.1LSNGAKK18 pKa = 10.26RR19 pKa = 11.84NGDD22 pKa = 2.67NKK24 pKa = 9.93MFHH27 pKa = 6.57TSPKK31 pKa = 9.68AIEE34 pKa = 4.0IGTINKK40 pKa = 9.85YY41 pKa = 10.16GVAGDD46 pKa = 4.4CLFFSDD52 pKa = 5.83DD53 pKa = 3.73IYY55 pKa = 11.26IMTASRR61 pKa = 11.84TCYY64 pKa = 10.29VYY66 pKa = 10.58EE67 pKa = 5.15ADD69 pKa = 4.1FDD71 pKa = 4.47CVRR74 pKa = 11.84ASQLYY79 pKa = 9.96DD80 pKa = 3.54DD81 pKa = 5.78EE82 pKa = 6.77IIADD86 pKa = 3.57IARR89 pKa = 11.84YY90 pKa = 9.43FDD92 pKa = 3.66VDD94 pKa = 3.16EE95 pKa = 4.6STAEE99 pKa = 4.03SLLDD103 pKa = 3.35GTQNEE108 pKa = 4.29WCLDD112 pKa = 3.67DD113 pKa = 5.49FDD115 pKa = 6.54CEE117 pKa = 5.18GEE119 pKa = 4.17NSWWLQGKK127 pKa = 9.96RR128 pKa = 11.84GEE130 pKa = 4.64CAVKK134 pKa = 9.78MGYY137 pKa = 9.76DD138 pKa = 3.25GCEE141 pKa = 4.25DD142 pKa = 3.79EE143 pKa = 6.04DD144 pKa = 4.1EE145 pKa = 4.34QGTVYY150 pKa = 10.25IVPMAGRR157 pKa = 11.84EE158 pKa = 4.22SEE160 pKa = 4.29LKK162 pKa = 10.7LVEE165 pKa = 4.43KK166 pKa = 10.58KK167 pKa = 10.61

Molecular weight: 18.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A513SNE2|A0A513SNE2_9CAUD Major capsid protein OS=Vibrio phage Va_90-11-287_p41_Ba35 OX=2565521 PE=4 SV=1
MM1 pKa = 7.32SCAIKK6 pKa = 9.8TKK8 pKa = 10.24NDD10 pKa = 3.68NIHH13 pKa = 5.93TPRR16 pKa = 11.84DD17 pKa = 3.38FRR19 pKa = 11.84GPRR22 pKa = 11.84NVFVNGNKK30 pKa = 9.64IKK32 pKa = 10.48FVVYY36 pKa = 10.76ADD38 pKa = 3.63TKK40 pKa = 10.11KK41 pKa = 11.1GIVKK45 pKa = 7.46YY46 pKa = 9.88HH47 pKa = 5.54PHH49 pKa = 5.85PVRR52 pKa = 11.84RR53 pKa = 11.84KK54 pKa = 7.87RR55 pKa = 11.84GSNVVYY61 pKa = 10.12TRR63 pKa = 11.84TLKK66 pKa = 11.14GLVTVEE72 pKa = 5.25FIRR75 pKa = 5.3

Molecular weight: 8.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

16871

53

1176

187.5

20.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.925 ± 0.388

1.428 ± 0.178

5.637 ± 0.256

6.443 ± 0.267

3.681 ± 0.19

6.988 ± 0.317

1.826 ± 0.225

6.633 ± 0.19

6.722 ± 0.32

7.789 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.786 ± 0.138

5.038 ± 0.19

3.782 ± 0.238

4.078 ± 0.256

4.837 ± 0.19

7.812 ± 0.306

5.388 ± 0.24

6.295 ± 0.245

1.517 ± 0.101

3.396 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski