Capybara microvirus Cap1_SP_116
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVP0|A0A4V1FVP0_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_116 OX=2584809 PE=4 SV=1
MM1 pKa = 8.11 PDD3 pKa = 3.69 LSNHH7 pKa = 6.7 AGSQVTPNPVTSGPYY22 pKa = 9.76 SVVNTADD29 pKa = 3.86 QLSGWNRR36 pKa = 11.84 LTDD39 pKa = 3.42 WLGVTNHH46 pKa = 5.99 QGQINNQLALQQSLLDD62 pKa = 4.7 DD63 pKa = 3.53 EE64 pKa = 5.46 RR65 pKa = 11.84 AYY67 pKa = 11.25 NDD69 pKa = 3.35 PAAQVEE75 pKa = 4.27 RR76 pKa = 11.84 LRR78 pKa = 11.84 NAGINPDD85 pKa = 3.6 LLGGVSSYY93 pKa = 11.44 EE94 pKa = 4.11 STGQGANPLSQPTGDD109 pKa = 3.58 FGNFILGVAQTFSGVASGILDD130 pKa = 4.08 LQSKK134 pKa = 10.46 ALDD137 pKa = 3.64 NDD139 pKa = 3.46 IKK141 pKa = 10.74 RR142 pKa = 11.84 ASLFDD147 pKa = 3.51 TEE149 pKa = 5.41 SILNRR154 pKa = 11.84 IASDD158 pKa = 3.48 RR159 pKa = 11.84 SISADD164 pKa = 3.79 FIEE167 pKa = 5.59 AMKK170 pKa = 10.73 DD171 pKa = 3.34 PNRR174 pKa = 11.84 LKK176 pKa = 10.66 PAVSLDD182 pKa = 3.28 QYY184 pKa = 11.62 VNLSTIRR191 pKa = 11.84 SKK193 pKa = 10.8 KK194 pKa = 8.03 VRR196 pKa = 11.84 KK197 pKa = 9.28 AVEE200 pKa = 3.87 DD201 pKa = 3.48 NFYY204 pKa = 11.35 SKK206 pKa = 10.77 LYY208 pKa = 10.61 SDD210 pKa = 6.04 DD211 pKa = 4.12 FIQRR215 pKa = 11.84 INGLLTGVNKK225 pKa = 10.01 SEE227 pKa = 4.33 RR228 pKa = 11.84 DD229 pKa = 3.45 FSEE232 pKa = 4.31 SVAGLRR238 pKa = 11.84 NPSKK242 pKa = 9.73 TADD245 pKa = 3.64 SLIDD249 pKa = 3.52 ALAEE253 pKa = 4.19 FMSDD257 pKa = 3.3 AYY259 pKa = 7.52 TTQMSANKK267 pKa = 10.14 AEE269 pKa = 4.13 RR270 pKa = 11.84 HH271 pKa = 6.28 HH272 pKa = 6.77 NDD274 pKa = 2.94 ASISQDD280 pKa = 3.63 DD281 pKa = 4.16 YY282 pKa = 11.99 NKK284 pKa = 9.92 MVTDD288 pKa = 4.88 ALDD291 pKa = 3.78 PSNEE295 pKa = 3.9 SDD297 pKa = 3.94 AITAGFEE304 pKa = 3.89 ASEE307 pKa = 4.26 TSKK310 pKa = 10.86 KK311 pKa = 9.68 KK312 pKa = 10.42 QSNIDD317 pKa = 3.55 SVIEE321 pKa = 3.98 EE322 pKa = 4.38 FTGKK326 pKa = 8.8 MKK328 pKa = 10.83 KK329 pKa = 9.82 KK330 pKa = 9.27 DD331 pKa = 3.65 TWWSDD336 pKa = 3.64 LILAIMAMYY345 pKa = 10.32 QSGAFDD351 pKa = 3.22 SFGFSRR357 pKa = 11.84 SSQSSFTPRR366 pKa = 11.84 GGSQEE371 pKa = 3.49 RR372 pKa = 11.84 SSFNFHH378 pKa = 6.92 LL379 pKa = 4.6
Molecular weight: 41.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.873
IPC2_protein 4.813
IPC_protein 4.774
Toseland 4.609
ProMoST 4.914
Dawson 4.749
Bjellqvist 4.902
Wikipedia 4.66
Rodwell 4.622
Grimsley 4.52
Solomon 4.749
Lehninger 4.711
Nozaki 4.863
DTASelect 5.092
Thurlkill 4.635
EMBOSS 4.685
Sillero 4.902
Patrickios 4.304
IPC_peptide 4.749
IPC2_peptide 4.889
IPC2.peptide.svr19 4.891
Protein with the highest isoelectric point:
>tr|A0A4P8W461|A0A4P8W461_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_116 OX=2584809 PE=4 SV=1
MM1 pKa = 7.64 SILALILSLVSLIRR15 pKa = 11.84 TGRR18 pKa = 11.84 NHH20 pKa = 7.12 RR21 pKa = 11.84 DD22 pKa = 2.93 VSRR25 pKa = 11.84 LVDD28 pKa = 3.77 TVAHH32 pKa = 6.18 IEE34 pKa = 3.8 EE35 pKa = 4.75 SIRR38 pKa = 11.84 VNDD41 pKa = 3.5 QAFVDD46 pKa = 3.81 ACKK49 pKa = 10.75 SFTEE53 pKa = 4.6 SIEE56 pKa = 4.06 KK57 pKa = 10.26 LDD59 pKa = 3.73 YY60 pKa = 11.07 ALVMLEE66 pKa = 4.8 RR67 pKa = 11.84 YY68 pKa = 6.69 TLPSGSSSLIVTGPRR83 pKa = 11.84 SKK85 pKa = 10.01 VTTTDD90 pKa = 3.06 SEE92 pKa = 5.49 SNMSVSDD99 pKa = 3.88 SRR101 pKa = 11.84 NQSSDD106 pKa = 3.04 KK107 pKa = 10.62 KK108 pKa = 10.99 SKK110 pKa = 10.69 NKK112 pKa = 9.97 NN113 pKa = 3.06
Molecular weight: 12.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.039
IPC2_protein 7.102
IPC_protein 7.775
Toseland 7.819
ProMoST 7.717
Dawson 8.214
Bjellqvist 7.892
Wikipedia 8.17
Rodwell 8.156
Grimsley 7.497
Solomon 8.697
Lehninger 8.697
Nozaki 8.229
DTASelect 8.17
Thurlkill 8.229
EMBOSS 8.492
Sillero 8.463
Patrickios 5.016
IPC_peptide 8.682
IPC2_peptide 7.366
IPC2.peptide.svr19 7.579
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1529
113
630
382.3
43.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.971 ± 1.451
1.57 ± 0.66
7.456 ± 0.709
3.924 ± 0.327
4.84 ± 0.479
5.298 ± 0.449
2.42 ± 0.371
5.298 ± 0.529
4.971 ± 1.105
8.698 ± 0.199
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.962 ± 0.121
6.802 ± 0.563
4.186 ± 0.732
4.382 ± 0.568
4.971 ± 0.443
11.445 ± 1.123
4.774 ± 0.854
5.755 ± 0.533
1.046 ± 0.182
5.232 ± 1.088
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here