Enterobacteria phage T3 (Bacteriophage T3)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Teetrevirus; Escherichia virus T3

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q778N3|Q778N3_BPT3 Portal protein OS=Enterobacteria phage T3 OX=10759 GN=gene 8 PE=3 SV=1
MM1 pKa = 7.7AEE3 pKa = 4.14SNADD7 pKa = 3.26VYY9 pKa = 11.77ASFGVNNAVMTGSTPTEE26 pKa = 4.18HH27 pKa = 6.27EE28 pKa = 4.14QNMLSLDD35 pKa = 3.43VAARR39 pKa = 11.84DD40 pKa = 3.57GDD42 pKa = 4.12DD43 pKa = 5.36AIVLSDD49 pKa = 4.4EE50 pKa = 4.42PTSHH54 pKa = 7.19NDD56 pKa = 3.04DD57 pKa = 4.37PYY59 pKa = 11.66AAGVDD64 pKa = 3.74PFADD68 pKa = 4.11GEE70 pKa = 4.21DD71 pKa = 3.6DD72 pKa = 3.43EE73 pKa = 6.17GRR75 pKa = 11.84IQVRR79 pKa = 11.84ISEE82 pKa = 4.82DD83 pKa = 3.32GNEE86 pKa = 4.28AGFDD90 pKa = 3.57TDD92 pKa = 3.53GDD94 pKa = 3.72NSEE97 pKa = 4.5VEE99 pKa = 4.38TEE101 pKa = 4.17GEE103 pKa = 4.04DD104 pKa = 4.46VEE106 pKa = 5.22FEE108 pKa = 4.26PLGDD112 pKa = 3.8TPEE115 pKa = 4.39EE116 pKa = 3.88LSQVTEE122 pKa = 3.53QLGQHH127 pKa = 5.61EE128 pKa = 5.22EE129 pKa = 4.43GFQAMVEE136 pKa = 4.22QAVEE140 pKa = 4.17RR141 pKa = 11.84GLSADD146 pKa = 3.48SVSRR150 pKa = 11.84IYY152 pKa = 10.67EE153 pKa = 3.94EE154 pKa = 4.57YY155 pKa = 9.69EE156 pKa = 3.59ADD158 pKa = 4.46GISEE162 pKa = 3.98KK163 pKa = 10.89SYY165 pKa = 11.82AEE167 pKa = 4.14LEE169 pKa = 3.96AAGYY173 pKa = 10.17SRR175 pKa = 11.84AFVDD179 pKa = 4.73SYY181 pKa = 11.66ISGQEE186 pKa = 3.89ALVDD190 pKa = 3.44QYY192 pKa = 11.99VNQVVAFAGGQEE204 pKa = 4.18RR205 pKa = 11.84FSAIHH210 pKa = 5.14THH212 pKa = 7.31LEE214 pKa = 3.98ATNPAAAEE222 pKa = 4.11SLEE225 pKa = 4.09SAMMNRR231 pKa = 11.84DD232 pKa = 3.32LATVKK237 pKa = 10.78AIINLAGEE245 pKa = 4.59SYY247 pKa = 9.79TKK249 pKa = 10.83KK250 pKa = 10.34FGKK253 pKa = 8.89PANRR257 pKa = 11.84SVTKK261 pKa = 10.23RR262 pKa = 11.84ATPVKK267 pKa = 9.56PVARR271 pKa = 11.84QKK273 pKa = 11.17EE274 pKa = 4.53GFTNQAEE281 pKa = 4.64MIKK284 pKa = 10.66AMSDD288 pKa = 2.81PRR290 pKa = 11.84YY291 pKa = 9.92RR292 pKa = 11.84SDD294 pKa = 2.87SAYY297 pKa = 10.7RR298 pKa = 11.84QMVEE302 pKa = 3.8QKK304 pKa = 10.93VIDD307 pKa = 3.65SSFF310 pKa = 2.9

Molecular weight: 33.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8W5U9|Q8W5U9_BPT3 0.3B protein OS=Enterobacteria phage T3 OX=10759 GN=gene 0.3B PE=4 SV=1
MM1 pKa = 6.98LTALIALVYY10 pKa = 10.17LCIAMYY16 pKa = 9.72FMRR19 pKa = 11.84DD20 pKa = 3.63FKK22 pKa = 11.03RR23 pKa = 11.84GLVVHH28 pKa = 7.04KK29 pKa = 10.02ATLSYY34 pKa = 9.68FKK36 pKa = 10.03WGFIPRR42 pKa = 11.84FTVRR46 pKa = 11.84EE47 pKa = 3.73RR48 pKa = 11.84NGRR51 pKa = 11.84FKK53 pKa = 11.15ANKK56 pKa = 9.51VGVFYY61 pKa = 10.74IATHH65 pKa = 5.88

Molecular weight: 7.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13344

25

1318

242.6

27.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.323 ± 0.435

1.147 ± 0.18

6.34 ± 0.252

6.954 ± 0.307

3.597 ± 0.225

7.509 ± 0.273

1.948 ± 0.187

4.984 ± 0.19

6.67 ± 0.361

8.266 ± 0.356

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.02 ± 0.183

4.227 ± 0.288

3.612 ± 0.205

3.927 ± 0.269

5.868 ± 0.234

5.883 ± 0.334

5.471 ± 0.281

6.572 ± 0.247

1.611 ± 0.157

3.073 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski