Clostridium phage phiMMP02

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Colneyvirus; Clostridioides virus phiMMP02

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9QDX5|J9QDX5_9CAUD Uncharacterized protein OS=Clostridium phage phiMMP02 OX=1204534 GN=phiMMP02_gp35 PE=4 SV=1
MM1 pKa = 7.8IYY3 pKa = 10.32LGNLMDD9 pKa = 4.92TEE11 pKa = 4.32EE12 pKa = 4.09QNIKK16 pKa = 9.14YY17 pKa = 10.31VGMIHH22 pKa = 6.29YY23 pKa = 7.62EE24 pKa = 3.96PNLLSEE30 pKa = 4.87EE31 pKa = 4.04NLKK34 pKa = 10.62QGILIEE40 pKa = 4.19SLPTQKK46 pKa = 10.66YY47 pKa = 10.18AEE49 pKa = 4.17NKK51 pKa = 7.51EE52 pKa = 3.86AKK54 pKa = 10.3LFINIDD60 pKa = 3.65TKK62 pKa = 10.86EE63 pKa = 3.8VFYY66 pKa = 10.48RR67 pKa = 11.84YY68 pKa = 8.2TDD70 pKa = 2.91IKK72 pKa = 11.4SNIEE76 pKa = 3.94DD77 pKa = 3.77KK78 pKa = 11.49VNSTEE83 pKa = 3.82QTIADD88 pKa = 3.92LTFQLMSNGVII99 pKa = 3.65

Molecular weight: 11.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9QE68|J9QE68_9CAUD Portal protein OS=Clostridium phage phiMMP02 OX=1204534 GN=phiMMP02_gp03 PE=4 SV=1
MM1 pKa = 7.74TDD3 pKa = 3.13QEE5 pKa = 5.61LRR7 pKa = 11.84LNWKK11 pKa = 8.78MLVKK15 pKa = 10.69EE16 pKa = 4.15NIKK19 pKa = 9.86VNNMKK24 pKa = 10.44KK25 pKa = 9.95FIYY28 pKa = 9.78INRR31 pKa = 11.84HH32 pKa = 3.4RR33 pKa = 11.84VYY35 pKa = 10.35IRR37 pKa = 11.84NLFKK41 pKa = 10.57ISAIYY46 pKa = 9.94KK47 pKa = 7.5EE48 pKa = 3.74NHH50 pKa = 6.4YY51 pKa = 10.57IRR53 pKa = 11.84EE54 pKa = 4.21FKK56 pKa = 10.81SEE58 pKa = 4.12CLSNNTLNAVIEE70 pKa = 4.28SSVRR74 pKa = 11.84IGFGSKK80 pKa = 9.76

Molecular weight: 9.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

14269

31

1424

187.8

21.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.389 ± 0.62

1.037 ± 0.139

5.663 ± 0.284

8.585 ± 0.562

3.707 ± 0.175

6.02 ± 0.512

0.904 ± 0.124

8.522 ± 0.365

10.659 ± 0.453

8.298 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.46 ± 0.156

7.064 ± 0.236

2.081 ± 0.143

3.189 ± 0.209

3.651 ± 0.25

6.223 ± 0.439

6.076 ± 0.339

5.782 ± 0.204

0.911 ± 0.101

3.777 ± 0.309
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski