Amino acid dipepetide frequency for Bacillus phage JL

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.176AlaAla: 2.176 ± 0.28
0.655AlaCys: 0.655 ± 0.13
4.025AlaAsp: 4.025 ± 0.356
4.259AlaGlu: 4.259 ± 0.288
2.106AlaPhe: 2.106 ± 0.259
4.212AlaGly: 4.212 ± 0.385
1.123AlaHis: 1.123 ± 0.14
4.867AlaIle: 4.867 ± 0.358
5.429AlaLys: 5.429 ± 0.401
5.663AlaLeu: 5.663 ± 0.354
1.778AlaMet: 1.778 ± 0.235
2.995AlaAsn: 2.995 ± 0.384
2.41AlaPro: 2.41 ± 0.237
2.2AlaGln: 2.2 ± 0.332
2.738AlaArg: 2.738 ± 0.268
3.463AlaSer: 3.463 ± 0.331
3.463AlaThr: 3.463 ± 0.455
4.306AlaVal: 4.306 ± 0.303
0.842AlaTrp: 0.842 ± 0.173
2.083AlaTyr: 2.083 ± 0.237
0.0AlaXaa: 0.0 ± 0.0
Cys
0.398CysAla: 0.398 ± 0.089
0.164CysCys: 0.164 ± 0.065
0.632CysAsp: 0.632 ± 0.133
0.632CysGlu: 0.632 ± 0.131
0.304CysPhe: 0.304 ± 0.082
0.702CysGly: 0.702 ± 0.156
0.164CysHis: 0.164 ± 0.06
0.889CysIle: 0.889 ± 0.15
0.842CysLys: 0.842 ± 0.142
0.913CysLeu: 0.913 ± 0.182
0.351CysMet: 0.351 ± 0.085
0.562CysAsn: 0.562 ± 0.109
0.491CysPro: 0.491 ± 0.133
0.304CysGln: 0.304 ± 0.087
0.468CysArg: 0.468 ± 0.102
0.702CysSer: 0.702 ± 0.115
0.772CysThr: 0.772 ± 0.161
0.491CysVal: 0.491 ± 0.099
0.211CysTrp: 0.211 ± 0.069
0.257CysTyr: 0.257 ± 0.094
0.0CysXaa: 0.0 ± 0.0
Asp
4.002AspAla: 4.002 ± 0.333
0.608AspCys: 0.608 ± 0.15
3.627AspAsp: 3.627 ± 0.275
4.774AspGlu: 4.774 ± 0.436
2.527AspPhe: 2.527 ± 0.218
5.008AspGly: 5.008 ± 0.375
1.521AspHis: 1.521 ± 0.267
4.61AspIle: 4.61 ± 0.282
5.64AspLys: 5.64 ± 0.412
5.803AspLeu: 5.803 ± 0.42
2.34AspMet: 2.34 ± 0.174
3.393AspAsn: 3.393 ± 0.278
2.364AspPro: 2.364 ± 0.359
1.638AspGln: 1.638 ± 0.178
2.598AspArg: 2.598 ± 0.266
3.206AspSer: 3.206 ± 0.283
3.674AspThr: 3.674 ± 0.357
4.376AspVal: 4.376 ± 0.359
1.17AspTrp: 1.17 ± 0.239
2.34AspTyr: 2.34 ± 0.233
0.0AspXaa: 0.0 ± 0.0
Glu
4.376GluAla: 4.376 ± 0.308
0.749GluCys: 0.749 ± 0.15
5.757GluAsp: 5.757 ± 0.461
7.652GluGlu: 7.652 ± 0.844
2.761GluPhe: 2.761 ± 0.208
5.476GluGly: 5.476 ± 0.356
2.247GluHis: 2.247 ± 0.255
4.376GluIle: 4.376 ± 0.317
5.242GluLys: 5.242 ± 0.419
6.201GluLeu: 6.201 ± 0.457
2.902GluMet: 2.902 ± 0.316
2.808GluAsn: 2.808 ± 0.253
2.176GluPro: 2.176 ± 0.28
2.925GluGln: 2.925 ± 0.305
3.44GluArg: 3.44 ± 0.397
3.51GluSer: 3.51 ± 0.296
3.136GluThr: 3.136 ± 0.246
6.248GluVal: 6.248 ± 0.502
1.404GluTrp: 1.404 ± 0.172
3.346GluTyr: 3.346 ± 0.288
0.0GluXaa: 0.0 ± 0.0
Phe
1.732PheAla: 1.732 ± 0.196
0.538PheCys: 0.538 ± 0.124
2.293PheAsp: 2.293 ± 0.242
2.176PheGlu: 2.176 ± 0.216
1.123PhePhe: 1.123 ± 0.213
2.457PheGly: 2.457 ± 0.243
0.796PheHis: 0.796 ± 0.162
2.106PheIle: 2.106 ± 0.241
2.644PheLys: 2.644 ± 0.239
2.832PheLeu: 2.832 ± 0.285
1.076PheMet: 1.076 ± 0.184
1.849PheAsn: 1.849 ± 0.211
1.287PhePro: 1.287 ± 0.187
0.866PheGln: 0.866 ± 0.139
1.872PheArg: 1.872 ± 0.239
2.598PheSer: 2.598 ± 0.218
2.434PheThr: 2.434 ± 0.271
2.059PheVal: 2.059 ± 0.219
0.445PheTrp: 0.445 ± 0.101
1.24PheTyr: 1.24 ± 0.154
0.0PheXaa: 0.0 ± 0.0
Gly
4.025GlyAla: 4.025 ± 0.353
0.679GlyCys: 0.679 ± 0.111
4.119GlyAsp: 4.119 ± 0.317
4.891GlyGlu: 4.891 ± 0.4
2.457GlyPhe: 2.457 ± 0.249
6.154GlyGly: 6.154 ± 0.865
1.381GlyHis: 1.381 ± 0.221
4.984GlyIle: 4.984 ± 0.305
5.382GlyLys: 5.382 ± 0.415
5.452GlyLeu: 5.452 ± 0.367
2.293GlyMet: 2.293 ± 0.281
3.768GlyAsn: 3.768 ± 0.484
0.257GlyPro: 0.257 ± 0.084
2.247GlyGln: 2.247 ± 0.237
3.276GlyArg: 3.276 ± 0.24
4.423GlySer: 4.423 ± 0.371
5.593GlyThr: 5.593 ± 0.709
5.312GlyVal: 5.312 ± 0.39
1.147GlyTrp: 1.147 ± 0.213
3.136GlyTyr: 3.136 ± 0.224
0.0GlyXaa: 0.0 ± 0.0
His
1.1HisAla: 1.1 ± 0.165
0.164HisCys: 0.164 ± 0.052
1.287HisAsp: 1.287 ± 0.176
1.498HisGlu: 1.498 ± 0.228
1.076HisPhe: 1.076 ± 0.16
1.661HisGly: 1.661 ± 0.236
0.702HisHis: 0.702 ± 0.153
1.474HisIle: 1.474 ± 0.211
1.638HisLys: 1.638 ± 0.235
1.825HisLeu: 1.825 ± 0.241
0.866HisMet: 0.866 ± 0.169
1.147HisAsn: 1.147 ± 0.16
1.24HisPro: 1.24 ± 0.177
0.515HisGln: 0.515 ± 0.111
1.03HisArg: 1.03 ± 0.169
1.287HisSer: 1.287 ± 0.194
1.287HisThr: 1.287 ± 0.173
1.264HisVal: 1.264 ± 0.168
0.351HisTrp: 0.351 ± 0.092
1.147HisTyr: 1.147 ± 0.19
0.0HisXaa: 0.0 ± 0.0
Ile
3.253IleAla: 3.253 ± 0.256
0.421IleCys: 0.421 ± 0.094
4.61IleAsp: 4.61 ± 0.375
5.148IleGlu: 5.148 ± 0.332
1.615IlePhe: 1.615 ± 0.153
4.048IleGly: 4.048 ± 0.292
1.521IleHis: 1.521 ± 0.213
3.534IleIle: 3.534 ± 0.316
5.008IleLys: 5.008 ± 0.313
4.423IleLeu: 4.423 ± 0.385
2.059IleMet: 2.059 ± 0.22
3.791IleAsn: 3.791 ± 0.444
2.878IlePro: 2.878 ± 0.271
2.457IleGln: 2.457 ± 0.216
3.534IleArg: 3.534 ± 0.312
3.463IleSer: 3.463 ± 0.393
4.516IleThr: 4.516 ± 0.334
4.119IleVal: 4.119 ± 0.306
0.515IleTrp: 0.515 ± 0.103
2.481IleTyr: 2.481 ± 0.254
0.0IleXaa: 0.0 ± 0.0
Lys
6.552LysAla: 6.552 ± 0.537
0.772LysCys: 0.772 ± 0.147
5.523LysAsp: 5.523 ± 0.331
7.442LysGlu: 7.442 ± 0.586
2.457LysPhe: 2.457 ± 0.252
6.084LysGly: 6.084 ± 0.325
1.919LysHis: 1.919 ± 0.231
3.791LysIle: 3.791 ± 0.308
6.81LysLys: 6.81 ± 0.717
5.593LysLeu: 5.593 ± 0.344
2.761LysMet: 2.761 ± 0.283
3.066LysAsn: 3.066 ± 0.301
3.276LysPro: 3.276 ± 0.292
2.925LysGln: 2.925 ± 0.294
3.604LysArg: 3.604 ± 0.4
4.493LysSer: 4.493 ± 0.378
3.814LysThr: 3.814 ± 0.305
5.523LysVal: 5.523 ± 0.299
1.287LysTrp: 1.287 ± 0.177
3.089LysTyr: 3.089 ± 0.236
0.0LysXaa: 0.0 ± 0.0
Leu
5.289LeuAla: 5.289 ± 0.32
0.725LeuCys: 0.725 ± 0.147
5.991LeuAsp: 5.991 ± 0.374
6.576LeuGlu: 6.576 ± 0.479
2.457LeuPhe: 2.457 ± 0.266
5.008LeuGly: 5.008 ± 0.33
1.544LeuHis: 1.544 ± 0.245
4.259LeuIle: 4.259 ± 0.359
6.201LeuLys: 6.201 ± 0.386
5.827LeuLeu: 5.827 ± 0.445
2.551LeuMet: 2.551 ± 0.315
3.276LeuAsn: 3.276 ± 0.245
3.346LeuPro: 3.346 ± 0.331
3.276LeuGln: 3.276 ± 0.303
3.276LeuArg: 3.276 ± 0.282
5.055LeuSer: 5.055 ± 0.438
4.587LeuThr: 4.587 ± 0.305
4.867LeuVal: 4.867 ± 0.441
1.006LeuTrp: 1.006 ± 0.166
3.042LeuTyr: 3.042 ± 0.258
0.0LeuXaa: 0.0 ± 0.0
Met
2.574MetAla: 2.574 ± 0.294
0.374MetCys: 0.374 ± 0.095
1.966MetAsp: 1.966 ± 0.229
2.13MetGlu: 2.13 ± 0.225
1.193MetPhe: 1.193 ± 0.181
2.387MetGly: 2.387 ± 0.33
0.491MetHis: 0.491 ± 0.125
1.778MetIle: 1.778 ± 0.19
2.785MetLys: 2.785 ± 0.31
2.434MetLeu: 2.434 ± 0.308
1.03MetMet: 1.03 ± 0.199
1.895MetAsn: 1.895 ± 0.203
1.006MetPro: 1.006 ± 0.145
0.959MetGln: 0.959 ± 0.153
1.474MetArg: 1.474 ± 0.171
2.434MetSer: 2.434 ± 0.267
1.568MetThr: 1.568 ± 0.174
2.223MetVal: 2.223 ± 0.226
0.328MetTrp: 0.328 ± 0.075
1.427MetTyr: 1.427 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
3.206AsnAla: 3.206 ± 0.517
0.538AsnCys: 0.538 ± 0.118
2.808AsnAsp: 2.808 ± 0.257
2.761AsnGlu: 2.761 ± 0.23
1.778AsnPhe: 1.778 ± 0.22
3.955AsnGly: 3.955 ± 0.461
1.053AsnHis: 1.053 ± 0.123
3.463AsnIle: 3.463 ± 0.28
3.978AsnLys: 3.978 ± 0.39
3.206AsnLeu: 3.206 ± 0.333
1.755AsnMet: 1.755 ± 0.216
2.504AsnAsn: 2.504 ± 0.271
2.598AsnPro: 2.598 ± 0.251
1.474AsnGln: 1.474 ± 0.194
2.293AsnArg: 2.293 ± 0.223
3.042AsnSer: 3.042 ± 0.239
2.598AsnThr: 2.598 ± 0.304
2.738AsnVal: 2.738 ± 0.253
0.866AsnTrp: 0.866 ± 0.148
2.223AsnTyr: 2.223 ± 0.254
0.0AsnXaa: 0.0 ± 0.0
Pro
2.527ProAla: 2.527 ± 0.258
0.374ProCys: 0.374 ± 0.091
2.457ProAsp: 2.457 ± 0.264
3.885ProGlu: 3.885 ± 0.336
1.03ProPhe: 1.03 ± 0.155
1.825ProGly: 1.825 ± 0.208
1.03ProHis: 1.03 ± 0.169
2.223ProIle: 2.223 ± 0.25
3.136ProLys: 3.136 ± 0.278
2.481ProLeu: 2.481 ± 0.234
0.983ProMet: 0.983 ± 0.14
1.802ProAsn: 1.802 ± 0.218
1.03ProPro: 1.03 ± 0.184
0.959ProGln: 0.959 ± 0.167
1.1ProArg: 1.1 ± 0.161
2.27ProSer: 2.27 ± 0.224
2.551ProThr: 2.551 ± 0.239
2.551ProVal: 2.551 ± 0.234
0.374ProTrp: 0.374 ± 0.083
1.404ProTyr: 1.404 ± 0.17
0.0ProXaa: 0.0 ± 0.0
Gln
2.059GlnAla: 2.059 ± 0.269
0.257GlnCys: 0.257 ± 0.078
1.802GlnAsp: 1.802 ± 0.19
2.855GlnGlu: 2.855 ± 0.253
1.498GlnPhe: 1.498 ± 0.184
2.644GlnGly: 2.644 ± 0.321
0.913GlnHis: 0.913 ± 0.143
1.919GlnIle: 1.919 ± 0.246
2.27GlnLys: 2.27 ± 0.21
3.112GlnLeu: 3.112 ± 0.271
1.334GlnMet: 1.334 ± 0.182
1.357GlnAsn: 1.357 ± 0.155
1.264GlnPro: 1.264 ± 0.229
1.334GlnGln: 1.334 ± 0.22
1.474GlnArg: 1.474 ± 0.18
1.685GlnSer: 1.685 ± 0.245
1.451GlnThr: 1.451 ± 0.197
2.2GlnVal: 2.2 ± 0.203
0.515GlnTrp: 0.515 ± 0.102
1.708GlnTyr: 1.708 ± 0.198
0.0GlnXaa: 0.0 ± 0.0
Arg
2.832ArgAla: 2.832 ± 0.243
0.421ArgCys: 0.421 ± 0.116
2.551ArgAsp: 2.551 ± 0.252
3.3ArgGlu: 3.3 ± 0.374
1.778ArgPhe: 1.778 ± 0.214
2.761ArgGly: 2.761 ± 0.252
0.959ArgHis: 0.959 ± 0.156
3.417ArgIle: 3.417 ± 0.312
4.048ArgLys: 4.048 ± 0.473
3.651ArgLeu: 3.651 ± 0.317
1.755ArgMet: 1.755 ± 0.227
2.036ArgAsn: 2.036 ± 0.222
1.427ArgPro: 1.427 ± 0.16
1.264ArgGln: 1.264 ± 0.184
2.574ArgArg: 2.574 ± 0.282
2.083ArgSer: 2.083 ± 0.227
2.481ArgThr: 2.481 ± 0.214
3.791ArgVal: 3.791 ± 0.355
0.562ArgTrp: 0.562 ± 0.129
1.755ArgTyr: 1.755 ± 0.199
0.0ArgXaa: 0.0 ± 0.0
Ser
3.697SerAla: 3.697 ± 0.385
0.515SerCys: 0.515 ± 0.111
3.908SerAsp: 3.908 ± 0.369
3.393SerGlu: 3.393 ± 0.297
2.223SerPhe: 2.223 ± 0.239
3.955SerGly: 3.955 ± 0.354
1.287SerHis: 1.287 ± 0.178
4.048SerIle: 4.048 ± 0.319
5.148SerLys: 5.148 ± 0.279
4.212SerLeu: 4.212 ± 0.304
2.176SerMet: 2.176 ± 0.193
2.808SerAsn: 2.808 ± 0.297
2.012SerPro: 2.012 ± 0.221
1.966SerGln: 1.966 ± 0.254
2.153SerArg: 2.153 ± 0.268
3.346SerSer: 3.346 ± 0.349
3.557SerThr: 3.557 ± 0.245
3.908SerVal: 3.908 ± 0.311
0.702SerTrp: 0.702 ± 0.159
2.27SerTyr: 2.27 ± 0.206
0.0SerXaa: 0.0 ± 0.0
Thr
3.557ThrAla: 3.557 ± 0.491
0.538ThrCys: 0.538 ± 0.12
3.159ThrAsp: 3.159 ± 0.264
4.306ThrGlu: 4.306 ± 0.304
2.247ThrPhe: 2.247 ± 0.249
4.75ThrGly: 4.75 ± 0.459
1.264ThrHis: 1.264 ± 0.156
3.674ThrIle: 3.674 ± 0.383
5.078ThrLys: 5.078 ± 0.326
4.75ThrLeu: 4.75 ± 0.351
1.1ThrMet: 1.1 ± 0.133
3.346ThrAsn: 3.346 ± 0.523
2.878ThrPro: 2.878 ± 0.255
1.872ThrGln: 1.872 ± 0.246
2.176ThrArg: 2.176 ± 0.197
3.159ThrSer: 3.159 ± 0.344
4.072ThrThr: 4.072 ± 0.569
4.68ThrVal: 4.68 ± 0.376
0.702ThrTrp: 0.702 ± 0.112
2.223ThrTyr: 2.223 ± 0.229
0.0ThrXaa: 0.0 ± 0.0
Val
4.189ValAla: 4.189 ± 0.309
0.842ValCys: 0.842 ± 0.151
5.172ValAsp: 5.172 ± 0.372
5.382ValGlu: 5.382 ± 0.406
1.802ValPhe: 1.802 ± 0.237
4.376ValGly: 4.376 ± 0.372
1.568ValHis: 1.568 ± 0.218
4.75ValIle: 4.75 ± 0.316
5.312ValLys: 5.312 ± 0.309
4.938ValLeu: 4.938 ± 0.337
1.872ValMet: 1.872 ± 0.217
3.534ValAsn: 3.534 ± 0.362
2.785ValPro: 2.785 ± 0.268
2.387ValGln: 2.387 ± 0.23
3.721ValArg: 3.721 ± 0.294
4.306ValSer: 4.306 ± 0.289
4.353ValThr: 4.353 ± 0.48
5.172ValVal: 5.172 ± 0.349
0.725ValTrp: 0.725 ± 0.126
2.574ValTyr: 2.574 ± 0.219
0.0ValXaa: 0.0 ± 0.0
Trp
0.866TrpAla: 0.866 ± 0.115
0.351TrpCys: 0.351 ± 0.108
0.983TrpAsp: 0.983 ± 0.148
1.006TrpGlu: 1.006 ± 0.157
0.491TrpPhe: 0.491 ± 0.117
0.959TrpGly: 0.959 ± 0.153
0.211TrpHis: 0.211 ± 0.059
0.749TrpIle: 0.749 ± 0.164
1.03TrpLys: 1.03 ± 0.145
1.03TrpLeu: 1.03 ± 0.175
0.445TrpMet: 0.445 ± 0.103
0.819TrpAsn: 0.819 ± 0.18
0.0TrpPro: 0.0 ± 0.0
0.679TrpGln: 0.679 ± 0.14
0.445TrpArg: 0.445 ± 0.097
0.749TrpSer: 0.749 ± 0.152
0.819TrpThr: 0.819 ± 0.166
1.381TrpVal: 1.381 ± 0.181
0.304TrpTrp: 0.304 ± 0.097
0.562TrpTyr: 0.562 ± 0.107
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.387TyrAla: 2.387 ± 0.255
0.562TyrCys: 0.562 ± 0.104
2.551TyrAsp: 2.551 ± 0.216
2.668TyrGlu: 2.668 ± 0.219
1.498TyrPhe: 1.498 ± 0.179
2.551TyrGly: 2.551 ± 0.276
0.866TyrHis: 0.866 ± 0.151
2.481TyrIle: 2.481 ± 0.237
3.159TyrLys: 3.159 ± 0.229
3.814TyrLeu: 3.814 ± 0.357
1.006TyrMet: 1.006 ± 0.161
2.083TyrAsn: 2.083 ± 0.27
1.264TyrPro: 1.264 ± 0.162
1.404TyrGln: 1.404 ± 0.156
2.2TyrArg: 2.2 ± 0.217
2.012TyrSer: 2.012 ± 0.225
2.808TyrThr: 2.808 ± 0.294
2.574TyrVal: 2.574 ± 0.229
0.421TyrTrp: 0.421 ± 0.105
1.568TyrTyr: 1.568 ± 0.224
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 218 proteins (42734 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski