Roseburia sp. CAG:100

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; environmental samples

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2901 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7R103|R7R103_9FIRM Pseudouridine synthase OS=Roseburia sp. CAG:100 OX=1262940 GN=BN450_01026 PE=3 SV=1
MM1 pKa = 7.8LEE3 pKa = 4.3TPVEE7 pKa = 4.22SLDD10 pKa = 3.63PQQATDD16 pKa = 3.57GTSFEE21 pKa = 4.7VIADD25 pKa = 3.82YY26 pKa = 11.2TDD28 pKa = 4.32GLMQMDD34 pKa = 4.94ADD36 pKa = 4.24GQAVPALAEE45 pKa = 4.41SYY47 pKa = 10.85DD48 pKa = 3.9LSDD51 pKa = 5.66DD52 pKa = 3.51GLTYY56 pKa = 10.34TFHH59 pKa = 8.08IRR61 pKa = 11.84DD62 pKa = 3.68DD63 pKa = 4.61ANWSNGTPVTAADD76 pKa = 5.8FVFAWQRR83 pKa = 11.84AADD86 pKa = 3.83PEE88 pKa = 4.46VASEE92 pKa = 3.86YY93 pKa = 10.9SYY95 pKa = 10.83MLSDD99 pKa = 3.75IGQIKK104 pKa = 9.57NAADD108 pKa = 3.98IIAGNKK114 pKa = 9.4DD115 pKa = 3.11KK116 pKa = 11.76SEE118 pKa = 4.54LGVTAVDD125 pKa = 4.28DD126 pKa = 3.6KK127 pKa = 11.27TLQVEE132 pKa = 4.37LNVPVSYY139 pKa = 10.22FLSLMYY145 pKa = 10.48FPTFYY150 pKa = 10.39PVNEE154 pKa = 4.34EE155 pKa = 4.29FFTSCGDD162 pKa = 3.56TFATSPEE169 pKa = 4.25TVLSNGAFVLDD180 pKa = 4.22SYY182 pKa = 11.36EE183 pKa = 3.99PAATAFHH190 pKa = 6.21LTKK193 pKa = 10.88NADD196 pKa = 3.57YY197 pKa = 11.02YY198 pKa = 11.4NADD201 pKa = 4.01SIQLAGLNYY210 pKa = 10.12QVIQDD215 pKa = 3.85SQQALMSYY223 pKa = 7.98QTGALDD229 pKa = 3.51TTLVNGEE236 pKa = 4.11QVDD239 pKa = 4.0QVKK242 pKa = 10.7DD243 pKa = 3.69DD244 pKa = 4.81PEE246 pKa = 4.16FQAIGAGYY254 pKa = 9.63LWYY257 pKa = 10.61VSPNIKK263 pKa = 10.04NVPEE267 pKa = 3.95LANLNIRR274 pKa = 11.84MALTMAINRR283 pKa = 11.84EE284 pKa = 4.26AITTDD289 pKa = 3.18VLKK292 pKa = 11.03DD293 pKa = 3.64GSTPTYY299 pKa = 8.31TAVPIEE305 pKa = 4.46FAAGPDD311 pKa = 3.62GSDD314 pKa = 3.67FSGDD318 pKa = 3.12QSKK321 pKa = 10.65FSDD324 pKa = 3.62VCAFDD329 pKa = 3.91ADD331 pKa = 3.7KK332 pKa = 11.09AAEE335 pKa = 3.98YY336 pKa = 8.67WQAGLDD342 pKa = 3.7EE343 pKa = 5.42LGVTEE348 pKa = 5.1LALDD352 pKa = 3.84MVVDD356 pKa = 5.47ADD358 pKa = 4.76DD359 pKa = 5.1APQKK363 pKa = 9.76VAQVLKK369 pKa = 9.23EE370 pKa = 3.9QWEE373 pKa = 4.5TTLPGLTVTLTIEE386 pKa = 4.16PKK388 pKa = 9.56KK389 pKa = 10.88QRR391 pKa = 11.84VQDD394 pKa = 3.73MQDD397 pKa = 2.95GNFQLGLTRR406 pKa = 11.84WGPDD410 pKa = 3.01YY411 pKa = 10.98ADD413 pKa = 3.44PMTYY417 pKa = 10.55LGMWVTDD424 pKa = 3.28NSNNYY429 pKa = 8.94GFWSNAEE436 pKa = 3.52YY437 pKa = 10.72DD438 pKa = 4.24AIIDD442 pKa = 3.72EE443 pKa = 4.83CTTGDD448 pKa = 3.77LCTDD452 pKa = 3.57AEE454 pKa = 4.59GRR456 pKa = 11.84WARR459 pKa = 11.84LYY461 pKa = 10.54DD462 pKa = 3.57AEE464 pKa = 5.01KK465 pKa = 10.45IVMDD469 pKa = 4.04EE470 pKa = 3.89AVIFPLYY477 pKa = 7.05TQCNAEE483 pKa = 4.11MMSSKK488 pKa = 9.18VTGVEE493 pKa = 4.08FHH495 pKa = 6.8PVALNRR501 pKa = 11.84VYY503 pKa = 11.1KK504 pKa = 10.67NAVKK508 pKa = 10.85AEE510 pKa = 3.93

Molecular weight: 56.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7RA20|R7RA20_9FIRM ABC transmembrane type-1 domain-containing protein OS=Roseburia sp. CAG:100 OX=1262940 GN=BN450_00576 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.71MTFQPKK8 pKa = 7.43TRR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.26VHH16 pKa = 5.59GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2901

0

2901

925423

29

1784

319.0

35.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.521 ± 0.051

1.482 ± 0.017

5.842 ± 0.035

7.522 ± 0.039

3.937 ± 0.029

6.708 ± 0.035

1.848 ± 0.023

7.596 ± 0.037

6.514 ± 0.039

8.704 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.285 ± 0.023

4.523 ± 0.029

3.11 ± 0.025

3.741 ± 0.032

4.212 ± 0.037

5.588 ± 0.037

5.536 ± 0.032

6.828 ± 0.033

0.957 ± 0.014

4.541 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski