Amino acid dipepetide frequency for Roseburia sp. CAG:100

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.13AlaAla: 7.13 ± 0.124	1.13AlaCys: 1.13 ± 0.034	4.799AlaAsp: 4.799 ± 0.082	4.816AlaGlu: 4.816 ± 0.082	3.006AlaPhe: 3.006 ± 0.069	5.928AlaGly: 5.928 ± 0.089	1.19AlaHis: 1.19 ± 0.037	5.309AlaIle: 5.309 ± 0.085	4.688AlaLys: 4.688 ± 0.087	6.762AlaLeu: 6.762 ± 0.097	2.507AlaMet: 2.507 ± 0.052	2.772AlaAsn: 2.772 ± 0.061	1.983AlaPro: 1.983 ± 0.048	2.952AlaGln: 2.952 ± 0.057	2.959AlaArg: 2.959 ± 0.055	3.975AlaSer: 3.975 ± 0.069	3.421AlaThr: 3.421 ± 0.065	6.044AlaVal: 6.044 ± 0.103	0.643AlaTrp: 0.643 ± 0.026	3.194AlaTyr: 3.194 ± 0.061	0.002AlaXaa: 0.002 ± 0.001
Cys	0.967CysAla: 0.967 ± 0.034	0.279CysCys: 0.279 ± 0.02	0.86CysAsp: 0.86 ± 0.035	0.926CysGlu: 0.926 ± 0.032	0.66CysPhe: 0.66 ± 0.025	1.386CysGly: 1.386 ± 0.041	0.351CysHis: 0.351 ± 0.021	1.204CysIle: 1.204 ± 0.045	0.871CysLys: 0.871 ± 0.03	1.131CysLeu: 1.131 ± 0.038	0.531CysMet: 0.531 ± 0.024	0.738CysAsn: 0.738 ± 0.028	0.526CysPro: 0.526 ± 0.026	0.412CysGln: 0.412 ± 0.02	0.672CysArg: 0.672 ± 0.027	0.835CysSer: 0.835 ± 0.028	0.702CysThr: 0.702 ± 0.031	0.997CysVal: 0.997 ± 0.031	0.121CysTrp: 0.121 ± 0.012	0.654CysTyr: 0.654 ± 0.028	0.0CysXaa: 0.0 ± 0.0
Asp	4.367AspAla: 4.367 ± 0.089	0.859AspCys: 0.859 ± 0.028	2.937AspAsp: 2.937 ± 0.071	4.836AspGlu: 4.836 ± 0.081	2.562AspPhe: 2.562 ± 0.056	4.121AspGly: 4.121 ± 0.08	0.869AspHis: 0.869 ± 0.031	4.986AspIle: 4.986 ± 0.079	4.142AspLys: 4.142 ± 0.078	4.094AspLeu: 4.094 ± 0.07	2.242AspMet: 2.242 ± 0.047	2.687AspAsn: 2.687 ± 0.055	1.523AspPro: 1.523 ± 0.045	1.337AspGln: 1.337 ± 0.036	2.422AspArg: 2.422 ± 0.055	3.262AspSer: 3.262 ± 0.06	3.585AspThr: 3.585 ± 0.071	3.954AspVal: 3.954 ± 0.072	0.652AspTrp: 0.652 ± 0.028	2.978AspTyr: 2.978 ± 0.064	0.002AspXaa: 0.002 ± 0.002
Glu	5.667GluAla: 5.667 ± 0.087	0.827GluCys: 0.827 ± 0.027	4.459GluAsp: 4.459 ± 0.088	7.266GluGlu: 7.266 ± 0.137	2.446GluPhe: 2.446 ± 0.049	4.169GluGly: 4.169 ± 0.075	1.451GluHis: 1.451 ± 0.044	5.548GluIle: 5.548 ± 0.079	6.083GluLys: 6.083 ± 0.097	6.851GluLeu: 6.851 ± 0.082	2.366GluMet: 2.366 ± 0.044	3.991GluAsn: 3.991 ± 0.073	1.838GluPro: 1.838 ± 0.048	3.54GluGln: 3.54 ± 0.069	2.956GluArg: 2.956 ± 0.058	3.43GluSer: 3.43 ± 0.064	3.563GluThr: 3.563 ± 0.064	4.493GluVal: 4.493 ± 0.07	0.708GluTrp: 0.708 ± 0.033	3.564GluTyr: 3.564 ± 0.071	0.002GluXaa: 0.002 ± 0.001
Phe	3.078PheAla: 3.078 ± 0.065	0.757PheCys: 0.757 ± 0.028	2.531PheAsp: 2.531 ± 0.052	2.387PheGlu: 2.387 ± 0.051	1.684PhePhe: 1.684 ± 0.053	2.811PheGly: 2.811 ± 0.068	0.896PheHis: 0.896 ± 0.033	2.733PheIle: 2.733 ± 0.066	1.896PheLys: 1.896 ± 0.048	3.639PheLeu: 3.639 ± 0.072	1.248PheMet: 1.248 ± 0.035	1.486PheAsn: 1.486 ± 0.046	1.285PhePro: 1.285 ± 0.042	1.323PheGln: 1.323 ± 0.038	1.595PheArg: 1.595 ± 0.038	2.498PheSer: 2.498 ± 0.061	2.396PheThr: 2.396 ± 0.055	2.758PheVal: 2.758 ± 0.062	0.492PheTrp: 0.492 ± 0.024	1.88PheTyr: 1.88 ± 0.05	0.001PheXaa: 0.001 ± 0.001
Gly	4.648GlyAla: 4.648 ± 0.084	1.229GlyCys: 1.229 ± 0.036	3.438GlyAsp: 3.438 ± 0.057	4.623GlyGlu: 4.623 ± 0.081	2.888GlyPhe: 2.888 ± 0.06	4.128GlyGly: 4.128 ± 0.088	1.246GlyHis: 1.246 ± 0.036	6.235GlyIle: 6.235 ± 0.099	4.776GlyLys: 4.776 ± 0.078	5.431GlyLeu: 5.431 ± 0.081	2.495GlyMet: 2.495 ± 0.059	3.145GlyAsn: 3.145 ± 0.068	1.094GlyPro: 1.094 ± 0.041	2.214GlyGln: 2.214 ± 0.05	3.065GlyArg: 3.065 ± 0.063	3.763GlySer: 3.763 ± 0.065	3.809GlyThr: 3.809 ± 0.071	4.729GlyVal: 4.729 ± 0.084	0.718GlyTrp: 0.718 ± 0.03	3.411GlyTyr: 3.411 ± 0.057	0.002GlyXaa: 0.002 ± 0.002
His	1.221HisAla: 1.221 ± 0.036	0.267HisCys: 0.267 ± 0.018	1.017HisAsp: 1.017 ± 0.037	1.195HisGlu: 1.195 ± 0.037	0.967HisPhe: 0.967 ± 0.029	1.258HisGly: 1.258 ± 0.045	0.474HisHis: 0.474 ± 0.032	1.513HisIle: 1.513 ± 0.041	1.154HisLys: 1.154 ± 0.042	1.458HisLeu: 1.458 ± 0.04	0.623HisMet: 0.623 ± 0.025	0.884HisAsn: 0.884 ± 0.028	0.835HisPro: 0.835 ± 0.03	0.586HisGln: 0.586 ± 0.027	0.804HisArg: 0.804 ± 0.029	0.981HisSer: 0.981 ± 0.036	1.045HisThr: 1.045 ± 0.034	1.148HisVal: 1.148 ± 0.038	0.196HisTrp: 0.196 ± 0.015	0.854HisTyr: 0.854 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	6.105IleAla: 6.105 ± 0.101	1.293IleCys: 1.293 ± 0.042	4.26IleAsp: 4.26 ± 0.063	5.172IleGlu: 5.172 ± 0.089	2.819IlePhe: 2.819 ± 0.059	5.068IleGly: 5.068 ± 0.094	1.436IleHis: 1.436 ± 0.04	5.432IleIle: 5.432 ± 0.095	3.977IleLys: 3.977 ± 0.07	7.052IleLeu: 7.052 ± 0.109	2.357IleMet: 2.357 ± 0.056	3.062IleAsn: 3.062 ± 0.056	3.175IlePro: 3.175 ± 0.065	2.627IleGln: 2.627 ± 0.05	3.666IleArg: 3.666 ± 0.069	4.86IleSer: 4.86 ± 0.076	4.685IleThr: 4.685 ± 0.067	5.081IleVal: 5.081 ± 0.076	0.714IleTrp: 0.714 ± 0.032	3.116IleTyr: 3.116 ± 0.067	0.001IleXaa: 0.001 ± 0.001
Lys	4.93LysAla: 4.93 ± 0.084	0.767LysCys: 0.767 ± 0.027	4.052LysAsp: 4.052 ± 0.067	6.442LysGlu: 6.442 ± 0.094	1.793LysPhe: 1.793 ± 0.046	4.098LysGly: 4.098 ± 0.079	1.038LysHis: 1.038 ± 0.034	4.469LysIle: 4.469 ± 0.07	5.248LysLys: 5.248 ± 0.091	5.445LysLeu: 5.445 ± 0.086	2.134LysMet: 2.134 ± 0.05	3.344LysAsn: 3.344 ± 0.058	1.872LysPro: 1.872 ± 0.05	2.444LysGln: 2.444 ± 0.053	2.718LysArg: 2.718 ± 0.057	3.018LysSer: 3.018 ± 0.062	3.414LysThr: 3.414 ± 0.064	4.308LysVal: 4.308 ± 0.076	0.638LysTrp: 0.638 ± 0.024	2.971LysTyr: 2.971 ± 0.056	0.0LysXaa: 0.0 ± 0.0
Leu	6.554LeuAla: 6.554 ± 0.1	1.497LeuCys: 1.497 ± 0.041	5.052LeuAsp: 5.052 ± 0.082	6.146LeuGlu: 6.146 ± 0.084	3.583LeuPhe: 3.583 ± 0.075	5.633LeuGly: 5.633 ± 0.071	1.792LeuHis: 1.792 ± 0.05	5.957LeuIle: 5.957 ± 0.095	5.286LeuLys: 5.286 ± 0.076	8.289LeuLeu: 8.289 ± 0.134	2.759LeuMet: 2.759 ± 0.052	3.703LeuAsn: 3.703 ± 0.066	3.257LeuPro: 3.257 ± 0.065	3.327LeuGln: 3.327 ± 0.06	3.55LeuArg: 3.55 ± 0.069	5.6LeuSer: 5.6 ± 0.087	5.125LeuThr: 5.125 ± 0.076	5.411LeuVal: 5.411 ± 0.082	0.76LeuTrp: 0.76 ± 0.026	3.76LeuTyr: 3.76 ± 0.074	0.0LeuXaa: 0.0 ± 0.0
Met	2.519MetAla: 2.519 ± 0.053	0.415MetCys: 0.415 ± 0.023	2.008MetAsp: 2.008 ± 0.048	2.65MetGlu: 2.65 ± 0.057	0.97MetPhe: 0.97 ± 0.033	2.077MetGly: 2.077 ± 0.053	0.587MetHis: 0.587 ± 0.024	2.497MetIle: 2.497 ± 0.051	2.556MetLys: 2.556 ± 0.054	2.822MetLeu: 2.822 ± 0.061	1.022MetMet: 1.022 ± 0.036	1.744MetAsn: 1.744 ± 0.045	1.171MetPro: 1.171 ± 0.032	1.412MetGln: 1.412 ± 0.041	1.309MetArg: 1.309 ± 0.042	1.843MetSer: 1.843 ± 0.043	1.867MetThr: 1.867 ± 0.048	2.06MetVal: 2.06 ± 0.048	0.249MetTrp: 0.249 ± 0.017	1.076MetTyr: 1.076 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	3.396AsnAla: 3.396 ± 0.054	0.604AsnCys: 0.604 ± 0.027	2.269AsnAsp: 2.269 ± 0.05	2.987AsnGlu: 2.987 ± 0.065	1.6AsnPhe: 1.6 ± 0.04	3.461AsnGly: 3.461 ± 0.073	0.938AsnHis: 0.938 ± 0.035	3.534AsnIle: 3.534 ± 0.069	2.633AsnLys: 2.633 ± 0.054	3.826AsnLeu: 3.826 ± 0.073	1.58AsnMet: 1.58 ± 0.048	2.02AsnAsn: 2.02 ± 0.054	1.953AsnPro: 1.953 ± 0.042	1.768AsnGln: 1.768 ± 0.045	2.094AsnArg: 2.094 ± 0.052	2.481AsnSer: 2.481 ± 0.059	2.534AsnThr: 2.534 ± 0.052	3.025AsnVal: 3.025 ± 0.058	0.464AsnTrp: 0.464 ± 0.023	2.065AsnTyr: 2.065 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	2.252ProAla: 2.252 ± 0.05	0.44ProCys: 0.44 ± 0.024	2.291ProAsp: 2.291 ± 0.055	2.785ProGlu: 2.785 ± 0.059	1.44ProPhe: 1.44 ± 0.041	1.874ProGly: 1.874 ± 0.047	0.559ProHis: 0.559 ± 0.027	2.128ProIle: 2.128 ± 0.053	1.831ProLys: 1.831 ± 0.054	2.533ProLeu: 2.533 ± 0.067	0.882ProMet: 0.882 ± 0.032	1.166ProAsn: 1.166 ± 0.035	0.634ProPro: 0.634 ± 0.025	1.313ProGln: 1.313 ± 0.045	1.015ProArg: 1.015 ± 0.036	1.641ProSer: 1.641 ± 0.043	1.523ProThr: 1.523 ± 0.044	2.925ProVal: 2.925 ± 0.056	0.34ProTrp: 0.34 ± 0.022	1.525ProTyr: 1.525 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	2.802GlnAla: 2.802 ± 0.055	0.433GlnCys: 0.433 ± 0.023	2.062GlnAsp: 2.062 ± 0.047	3.34GlnGlu: 3.34 ± 0.066	1.256GlnPhe: 1.256 ± 0.036	2.291GlnGly: 2.291 ± 0.048	0.533GlnHis: 0.533 ± 0.025	3.067GlnIle: 3.067 ± 0.058	2.758GlnLys: 2.758 ± 0.063	3.093GlnLeu: 3.093 ± 0.064	1.34GlnMet: 1.34 ± 0.036	1.921GlnAsn: 1.921 ± 0.053	1.06GlnPro: 1.06 ± 0.042	1.638GlnGln: 1.638 ± 0.046	1.353GlnArg: 1.353 ± 0.039	1.918GlnSer: 1.918 ± 0.05	2.097GlnThr: 2.097 ± 0.057	2.269GlnVal: 2.269 ± 0.051	0.374GlnTrp: 0.374 ± 0.02	1.807GlnTyr: 1.807 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	2.549ArgAla: 2.549 ± 0.052	0.551ArgCys: 0.551 ± 0.024	2.173ArgAsp: 2.173 ± 0.058	3.559ArgGlu: 3.559 ± 0.057	1.731ArgPhe: 1.731 ± 0.049	2.177ArgGly: 2.177 ± 0.045	0.777ArgHis: 0.777 ± 0.035	3.559ArgIle: 3.559 ± 0.058	3.375ArgLys: 3.375 ± 0.067	3.696ArgLeu: 3.696 ± 0.068	1.532ArgMet: 1.532 ± 0.043	2.281ArgAsn: 2.281 ± 0.055	1.212ArgPro: 1.212 ± 0.043	1.855ArgGln: 1.855 ± 0.054	2.097ArgArg: 2.097 ± 0.063	2.064ArgSer: 2.064 ± 0.043	2.134ArgThr: 2.134 ± 0.046	2.553ArgVal: 2.553 ± 0.056	0.347ArgTrp: 0.347 ± 0.018	1.891ArgTyr: 1.891 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	4.095SerAla: 4.095 ± 0.065	0.775SerCys: 0.775 ± 0.031	3.306SerAsp: 3.306 ± 0.061	3.58SerGlu: 3.58 ± 0.058	2.611SerPhe: 2.611 ± 0.053	4.388SerGly: 4.388 ± 0.075	0.984SerHis: 0.984 ± 0.037	4.252SerIle: 4.252 ± 0.066	3.298SerLys: 3.298 ± 0.069	4.909SerLeu: 4.909 ± 0.069	1.892SerMet: 1.892 ± 0.05	2.344SerAsn: 2.344 ± 0.051	1.527SerPro: 1.527 ± 0.04	2.006SerGln: 2.006 ± 0.047	2.498SerArg: 2.498 ± 0.041	3.307SerSer: 3.307 ± 0.071	3.032SerThr: 3.032 ± 0.057	3.958SerVal: 3.958 ± 0.067	0.512SerTrp: 0.512 ± 0.024	2.609SerTyr: 2.609 ± 0.052	0.001SerXaa: 0.001 ± 0.001
Thr	4.328ThrAla: 4.328 ± 0.068	0.653ThrCys: 0.653 ± 0.027	3.523ThrAsp: 3.523 ± 0.07	3.729ThrGlu: 3.729 ± 0.071	2.167ThrPhe: 2.167 ± 0.053	4.457ThrGly: 4.457 ± 0.075	0.957ThrHis: 0.957 ± 0.036	4.134ThrIle: 4.134 ± 0.068	3.033ThrLys: 3.033 ± 0.058	4.909ThrLeu: 4.909 ± 0.079	1.5ThrMet: 1.5 ± 0.044	2.176ThrAsn: 2.176 ± 0.048	2.162ThrPro: 2.162 ± 0.051	2.131ThrGln: 2.131 ± 0.055	2.05ThrArg: 2.05 ± 0.05	3.035ThrSer: 3.035 ± 0.059	3.168ThrThr: 3.168 ± 0.066	4.341ThrVal: 4.341 ± 0.076	0.481ThrTrp: 0.481 ± 0.022	2.427ThrTyr: 2.427 ± 0.056	0.001ThrXaa: 0.001 ± 0.001
Val	4.77ValAla: 4.77 ± 0.09	1.172ValCys: 1.172 ± 0.037	3.843ValAsp: 3.843 ± 0.078	4.655ValGlu: 4.655 ± 0.075	2.808ValPhe: 2.808 ± 0.063	4.035ValGly: 4.035 ± 0.077	1.091ValHis: 1.091 ± 0.035	5.513ValIle: 5.513 ± 0.088	4.452ValLys: 4.452 ± 0.08	6.271ValLeu: 6.271 ± 0.106	2.185ValMet: 2.185 ± 0.048	3.121ValAsn: 3.121 ± 0.057	2.234ValPro: 2.234 ± 0.052	2.208ValGln: 2.208 ± 0.054	2.725ValArg: 2.725 ± 0.058	4.424ValSer: 4.424 ± 0.059	4.297ValThr: 4.297 ± 0.078	4.86ValVal: 4.86 ± 0.092	0.645ValTrp: 0.645 ± 0.027	2.969ValTyr: 2.969 ± 0.06	0.002ValXaa: 0.002 ± 0.002
Trp	0.581TrpAla: 0.581 ± 0.028	0.172TrpCys: 0.172 ± 0.013	0.569TrpAsp: 0.569 ± 0.028	0.658TrpGlu: 0.658 ± 0.028	0.417TrpPhe: 0.417 ± 0.024	0.681TrpGly: 0.681 ± 0.027	0.218TrpHis: 0.218 ± 0.015	0.686TrpIle: 0.686 ± 0.027	0.732TrpLys: 0.732 ± 0.026	0.884TrpLeu: 0.884 ± 0.032	0.334TrpMet: 0.334 ± 0.021	0.644TrpAsn: 0.644 ± 0.029	0.193TrpPro: 0.193 ± 0.016	0.433TrpGln: 0.433 ± 0.022	0.391TrpArg: 0.391 ± 0.02	0.484TrpSer: 0.484 ± 0.025	0.426TrpThr: 0.426 ± 0.021	0.519TrpVal: 0.519 ± 0.027	0.112TrpTrp: 0.112 ± 0.012	0.433TrpTyr: 0.433 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.251TyrAla: 3.251 ± 0.072	0.705TyrCys: 0.705 ± 0.028	2.968TyrAsp: 2.968 ± 0.063	3.464TyrGlu: 3.464 ± 0.073	1.96TyrPhe: 1.96 ± 0.039	3.042TyrGly: 3.042 ± 0.055	1.09TyrHis: 1.09 ± 0.034	3.203TyrIle: 3.203 ± 0.061	2.355TyrLys: 2.355 ± 0.049	3.943TyrLeu: 3.943 ± 0.083	1.324TyrMet: 1.324 ± 0.04	2.002TyrAsn: 2.002 ± 0.053	1.561TyrPro: 1.561 ± 0.044	1.886TyrGln: 1.886 ± 0.047	2.175TyrArg: 2.175 ± 0.05	2.463TyrSer: 2.463 ± 0.058	2.537TyrThr: 2.537 ± 0.055	2.85TyrVal: 2.85 ± 0.052	0.405TyrTrp: 0.405 ± 0.021	2.227TyrTyr: 2.227 ± 0.06	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.002XaaPhe: 0.002 ± 0.002	0.002XaaGly: 0.002 ± 0.002	0.001XaaHis: 0.001 ± 0.001	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.002	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.022XaaXaa: 0.022 ± 0.006

Statistics based on 2901 proteins (925423 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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