Equus caballus papillomavirus 3
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7C0H6|E7C0H6_9PAPI Major capsid protein L1 OS=Equus caballus papillomavirus 3 OX=940834 GN=L1 PE=3 SV=1
MM1 pKa = 7.1 TSGRR5 pKa = 11.84 RR6 pKa = 11.84 KK7 pKa = 9.55 RR8 pKa = 11.84 RR9 pKa = 11.84 AADD12 pKa = 3.51 TTDD15 pKa = 3.03 TPPRR19 pKa = 11.84 KK20 pKa = 9.3 RR21 pKa = 11.84 RR22 pKa = 11.84 PRR24 pKa = 11.84 AAVEE28 pKa = 4.11 DD29 pKa = 4.33 IYY31 pKa = 11.17 RR32 pKa = 11.84 GCKK35 pKa = 9.21 PFNTCPEE42 pKa = 4.13 DD43 pKa = 3.46 VVNRR47 pKa = 11.84 VEE49 pKa = 4.1 NKK51 pKa = 7.59 TWADD55 pKa = 4.73 RR56 pKa = 11.84 LLQWLGSVIYY66 pKa = 10.66 LGGLGIGSGRR76 pKa = 11.84 GTGGGTGYY84 pKa = 10.72 RR85 pKa = 11.84 PLGSTNRR92 pKa = 11.84 GVVVGGGSRR101 pKa = 11.84 AVRR104 pKa = 11.84 PPPLVVDD111 pKa = 4.33 TIGPVDD117 pKa = 3.81 AEE119 pKa = 4.29 VLEE122 pKa = 4.72 MIPLQPLEE130 pKa = 4.37 PGGPSVVSGSDD141 pKa = 2.97 GGVLVSEE148 pKa = 5.19 GGSLPPEE155 pKa = 4.07 IPGVLDD161 pKa = 3.42 GGGPEE166 pKa = 4.3 DD167 pKa = 5.1 SIPARR172 pKa = 11.84 DD173 pKa = 3.62 PAVLQVGEE181 pKa = 4.24 GDD183 pKa = 3.44 TGNSHH188 pKa = 7.35 VITPAIEE195 pKa = 3.92 RR196 pKa = 11.84 PGFSGSSRR204 pKa = 11.84 DD205 pKa = 2.87 SFYY208 pKa = 10.78 IVDD211 pKa = 3.61 SGSRR215 pKa = 11.84 GSVVGEE221 pKa = 4.38 DD222 pKa = 3.52 IEE224 pKa = 5.82 LVDD227 pKa = 5.48 LPRR230 pKa = 11.84 ASTPEE235 pKa = 3.99 TTGDD239 pKa = 3.34 ALTRR243 pKa = 11.84 GLGSRR248 pKa = 11.84 KK249 pKa = 8.24 YY250 pKa = 8.36 QQVYY254 pKa = 9.29 VEE256 pKa = 4.91 NPAFVYY262 pKa = 10.81 NPTEE266 pKa = 3.8 LVAFGDD272 pKa = 3.87 TWDD275 pKa = 3.92 GATDD279 pKa = 3.57 SFSYY283 pKa = 10.88 DD284 pKa = 3.41 PVVASPQAAPDD295 pKa = 3.64 EE296 pKa = 4.54 LFTDD300 pKa = 4.11 IVHH303 pKa = 7.51 LGRR306 pKa = 11.84 QMYY309 pKa = 9.68 EE310 pKa = 3.69 RR311 pKa = 11.84 GRR313 pKa = 11.84 EE314 pKa = 3.74 GLLRR318 pKa = 11.84 VGRR321 pKa = 11.84 VGRR324 pKa = 11.84 RR325 pKa = 11.84 GTIQTRR331 pKa = 11.84 AGTQIGPQVHH341 pKa = 6.66 FFHH344 pKa = 7.51 DD345 pKa = 4.74 LSPILPVQEE354 pKa = 4.28 EE355 pKa = 4.81 VEE357 pKa = 4.32 LTTFPRR363 pKa = 11.84 APEE366 pKa = 3.98 FEE368 pKa = 4.45 STNSEE373 pKa = 3.57 EE374 pKa = 4.31 TAFTEE379 pKa = 4.55 VDD381 pKa = 3.82 LQSEE385 pKa = 4.33 PSSYY389 pKa = 11.13 SDD391 pKa = 3.22 TYY393 pKa = 11.22 LVEE396 pKa = 5.22 DD397 pKa = 4.83 DD398 pKa = 4.17 SVSVTGHH405 pKa = 6.54 LVFSDD410 pKa = 3.48 EE411 pKa = 4.83 GGSLTDD417 pKa = 3.41 NVHH420 pKa = 7.22 PINVPLRR427 pKa = 11.84 GSAGVGLLSVQGTTDD442 pKa = 3.26 NLTSNSLTPALQPPQPGGGDD462 pKa = 3.19 RR463 pKa = 11.84 EE464 pKa = 4.64 VVVNMYY470 pKa = 9.42 PYY472 pKa = 10.79 SIMSFLHH479 pKa = 4.2 YY480 pKa = 9.76 RR481 pKa = 11.84 RR482 pKa = 11.84 RR483 pKa = 11.84 RR484 pKa = 11.84 KK485 pKa = 9.5 RR486 pKa = 11.84 GYY488 pKa = 10.24 VYY490 pKa = 10.6 FSDD493 pKa = 4.34 VILAII498 pKa = 4.24
Molecular weight: 53.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.843
IPC2_protein 4.838
IPC_protein 4.787
Toseland 4.647
ProMoST 4.902
Dawson 4.749
Bjellqvist 4.889
Wikipedia 4.622
Rodwell 4.635
Grimsley 4.558
Solomon 4.749
Lehninger 4.698
Nozaki 4.851
DTASelect 5.029
Thurlkill 4.647
EMBOSS 4.635
Sillero 4.914
Patrickios 4.075
IPC_peptide 4.749
IPC2_peptide 4.902
IPC2.peptide.svr19 4.9
Protein with the highest isoelectric point:
>tr|E7C0H5|E7C0H5_9PAPI Minor capsid protein L2 OS=Equus caballus papillomavirus 3 OX=940834 GN=L2 PE=3 SV=1
II1 pKa = 7.28 PKK3 pKa = 10.15 GYY5 pKa = 8.95 TIVRR9 pKa = 11.84 GPLKK13 pKa = 10.66 SIMRR17 pKa = 11.84 TLQLKK22 pKa = 9.72 QKK24 pKa = 9.13 STLKK28 pKa = 10.69 QMFGKK33 pKa = 10.1 SCFKK37 pKa = 10.22 TKK39 pKa = 10.5 HH40 pKa = 5.74 FLLSPAEE47 pKa = 4.08 LPRR50 pKa = 11.84 RR51 pKa = 11.84 TPQSLLDD58 pKa = 3.93 LAYY61 pKa = 10.36 QAATSRR67 pKa = 11.84 TAQQRR72 pKa = 11.84 SPVYY76 pKa = 10.08 FPLRR80 pKa = 11.84 LPPCLLNSSLYY91 pKa = 10.12 QRR93 pKa = 11.84 NCYY96 pKa = 10.07 RR97 pKa = 11.84 EE98 pKa = 4.12 VLRR101 pKa = 11.84 GTAEE105 pKa = 4.04 ALGEE109 pKa = 4.08 AAHH112 pKa = 6.16 TGAWGPLPMSPVPGPYY128 pKa = 10.18 ARR130 pKa = 11.84 GEE132 pKa = 4.08 LDD134 pKa = 3.91 SYY136 pKa = 11.1 LPPPACRR143 pKa = 11.84 RR144 pKa = 11.84 LYY146 pKa = 10.28 RR147 pKa = 11.84 AQLYY151 pKa = 8.61 HH152 pKa = 5.93 QRR154 pKa = 11.84 QVLLLYY160 pKa = 9.88 LQYY163 pKa = 10.84 YY164 pKa = 7.6 PQVDD168 pKa = 3.99 AGPPAGPP175 pKa = 3.83
Molecular weight: 19.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.67
IPC2_protein 9.75
IPC_protein 10.218
Toseland 9.926
ProMoST 9.838
Dawson 10.233
Bjellqvist 10.014
Wikipedia 10.482
Rodwell 10.423
Grimsley 10.35
Solomon 10.277
Lehninger 10.218
Nozaki 9.999
DTASelect 9.999
Thurlkill 10.058
EMBOSS 10.365
Sillero 10.16
Patrickios 5.105
IPC_peptide 10.262
IPC2_peptide 9.092
IPC2.peptide.svr19 8.554
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2456
93
621
350.9
39.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.067 ± 0.73
2.484 ± 0.562
5.7 ± 0.61
5.619 ± 0.4
3.379 ± 0.296
7.573 ± 1.106
2.239 ± 0.313
3.176 ± 0.262
4.56 ± 0.818
8.958 ± 0.709
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.954 ± 0.314
2.972 ± 0.378
7.085 ± 0.691
4.723 ± 0.558
6.678 ± 0.56
8.103 ± 0.55
5.863 ± 0.621
7.329 ± 0.705
1.792 ± 0.412
3.746 ± 0.39
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here