Enhydrobacter aerosaccus
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6507 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T4TH63|A0A1T4TH63_9PROT Serine--tRNA ligase OS=Enhydrobacter aerosaccus OX=225324 GN=serS PE=3 SV=1
MM1 pKa = 7.56 IGGAGNDD8 pKa = 3.37 TYY10 pKa = 11.75 YY11 pKa = 11.1 VDD13 pKa = 3.68 NPGDD17 pKa = 3.59 IVRR20 pKa = 11.84 EE21 pKa = 3.94 TAGAAFAVPAGWTLKK36 pKa = 9.3 GTADD40 pKa = 3.6 YY41 pKa = 11.4 NGDD44 pKa = 3.59 GEE46 pKa = 4.93 LDD48 pKa = 3.75 VVVSQGNTNQLWLLSGGALQSTVALPDD75 pKa = 3.77 LSAGGWQFLGIADD88 pKa = 4.11 EE89 pKa = 4.9 NGDD92 pKa = 3.65 GTKK95 pKa = 10.84 DD96 pKa = 3.03 LLYY99 pKa = 10.16 RR100 pKa = 11.84 HH101 pKa = 6.8 TYY103 pKa = 9.27 LGIYY107 pKa = 9.57 YY108 pKa = 9.27 SQLMNGATVGGGANAPVTTPDD129 pKa = 3.59 PLQPLTASNQGNDD142 pKa = 3.55 TVISSITYY150 pKa = 9.25 ALTAGVEE157 pKa = 4.14 NLTLAAGAGAIDD169 pKa = 3.51 ATGNAVANLIVGNEE183 pKa = 3.88 GNNRR187 pKa = 11.84 ITGGGGADD195 pKa = 3.66 TLTGGAGNDD204 pKa = 3.39 TFVFTAASDD213 pKa = 3.97 STPAAADD220 pKa = 3.83 TITDD224 pKa = 4.5 FTQGSDD230 pKa = 3.77 SLDD233 pKa = 3.28 LSAIGQFRR241 pKa = 11.84 WLGTAAFDD249 pKa = 3.65 HH250 pKa = 6.17 QANALHH256 pKa = 6.1 YY257 pKa = 9.59 ASAGGITTLSADD269 pKa = 3.26 INGDD273 pKa = 3.31 GVADD277 pKa = 3.99 FAVNLTGTLPLTTADD292 pKa = 3.71 FTAASIAGASSAVMTPMDD310 pKa = 4.15 AQSAQLIQAMATFDD324 pKa = 3.39 TTAAGQSTLSAMPQDD339 pKa = 3.77 STTIPLAVNHH349 pKa = 6.59 HH350 pKa = 5.75 NAA352 pKa = 3.55
Molecular weight: 35.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.605
IPC_protein 3.643
Toseland 3.401
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.656
Rodwell 3.465
Grimsley 3.312
Solomon 3.656
Lehninger 3.617
Nozaki 3.783
DTASelect 4.101
Thurlkill 3.478
EMBOSS 3.656
Sillero 3.77
Patrickios 0.769
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.724
Protein with the highest isoelectric point:
>tr|A0A1T4QP33|A0A1T4QP33_9PROT Heme chaperone HemW OS=Enhydrobacter aerosaccus OX=225324 GN=SAMN02745126_03356 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.29 QPSKK9 pKa = 10.24 LIRR12 pKa = 11.84 KK13 pKa = 8.44 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.45 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.33 VIANRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 SGRR39 pKa = 11.84 KK40 pKa = 8.96 RR41 pKa = 11.84 LSAA44 pKa = 4.09
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6507
0
6507
2051812
39
2453
315.3
34.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.378 ± 0.037
0.888 ± 0.008
5.467 ± 0.024
5.267 ± 0.025
3.705 ± 0.02
8.645 ± 0.031
2.082 ± 0.015
4.951 ± 0.019
3.38 ± 0.025
10.317 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.46 ± 0.013
2.49 ± 0.017
5.508 ± 0.02
3.159 ± 0.016
7.313 ± 0.028
5.238 ± 0.022
5.283 ± 0.025
7.669 ± 0.03
1.481 ± 0.013
2.317 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here