Friedmanniella luteola
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4260 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H1ZQI5|A0A1H1ZQI5_9ACTN CBS domain-containing protein OS=Friedmanniella luteola OX=546871 GN=SAMN04488543_3944 PE=4 SV=1
MM1 pKa = 6.49 TVAMAAQEE9 pKa = 4.28 FEE11 pKa = 5.18 LLVQQALDD19 pKa = 3.86 EE20 pKa = 4.64 VPDD23 pKa = 3.68 EE24 pKa = 4.36 LLALVEE30 pKa = 4.11 NCVILVEE37 pKa = 4.69 DD38 pKa = 5.14 APPPDD43 pKa = 3.49 LAGLLGLYY51 pKa = 10.44 DD52 pKa = 4.05 GVPLTEE58 pKa = 5.17 RR59 pKa = 11.84 GDD61 pKa = 3.91 FYY63 pKa = 11.66 AGVLPDD69 pKa = 4.82 RR70 pKa = 11.84 ILIYY74 pKa = 10.24 RR75 pKa = 11.84 LPILAVCDD83 pKa = 3.43 THH85 pKa = 7.69 EE86 pKa = 4.45 DD87 pKa = 3.65 VVEE90 pKa = 4.22 EE91 pKa = 3.77 VHH93 pKa = 5.75 ITVVHH98 pKa = 6.69 EE99 pKa = 4.08 IAHH102 pKa = 6.16 HH103 pKa = 6.46 FGIDD107 pKa = 3.52 DD108 pKa = 4.21 DD109 pKa = 4.45 RR110 pKa = 11.84 LHH112 pKa = 6.48 EE113 pKa = 4.68 LGYY116 pKa = 10.74 DD117 pKa = 3.3
Molecular weight: 12.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.973
IPC_protein 3.935
Toseland 3.745
ProMoST 4.024
Dawson 3.91
Bjellqvist 4.101
Wikipedia 3.834
Rodwell 3.77
Grimsley 3.656
Solomon 3.91
Lehninger 3.859
Nozaki 4.037
DTASelect 4.228
Thurlkill 3.783
EMBOSS 3.846
Sillero 4.062
Patrickios 1.036
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.96
Protein with the highest isoelectric point:
>tr|A0A1H1LWI3|A0A1H1LWI3_9ACTN Haloacid dehalogenase superfamily subfamily IA variant 3 with third motif having DD or ED/beta-phosphoglucomutase family hydrolase OS=Friedmanniella luteola OX=546871 GN=SAMN04488543_0473 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.72 HH17 pKa = 5.74 RR18 pKa = 11.84 KK19 pKa = 7.87 LLKK22 pKa = 8.37 RR23 pKa = 11.84 TRR25 pKa = 11.84 IQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 AGKK33 pKa = 9.79
Molecular weight: 4.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4260
0
4260
1392974
29
5196
327.0
34.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.0 ± 0.05
0.664 ± 0.01
6.171 ± 0.035
5.145 ± 0.035
2.682 ± 0.025
9.452 ± 0.036
2.113 ± 0.019
2.709 ± 0.032
1.54 ± 0.027
10.89 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.526 ± 0.014
1.551 ± 0.019
6.157 ± 0.03
2.917 ± 0.022
8.019 ± 0.05
5.121 ± 0.029
6.203 ± 0.053
9.735 ± 0.035
1.524 ± 0.016
1.881 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here