Beihai narna-like virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KIK3|A0A1L3KIK3_9VIRU Uncharacterized protein OS=Beihai narna-like virus 9 OX=1922461 PE=4 SV=1
MM1 pKa = 7.79SPEE4 pKa = 4.66DD5 pKa = 4.59LPACLEE11 pKa = 3.92RR12 pKa = 11.84AARR15 pKa = 11.84ALAGRR20 pKa = 11.84QKK22 pKa = 9.39WLHH25 pKa = 5.1MKK27 pKa = 9.88HH28 pKa = 6.41RR29 pKa = 11.84SFVALTNGAYY39 pKa = 10.26FLRR42 pKa = 11.84SLVEE46 pKa = 3.96VLLDD50 pKa = 3.96ADD52 pKa = 4.9PIFLRR57 pKa = 11.84LKK59 pKa = 10.65LADD62 pKa = 3.7FWEE65 pKa = 4.7VFSILWPISPAEE77 pKa = 3.79QVAVLKK83 pKa = 10.81YY84 pKa = 8.47WVAWPMARR92 pKa = 11.84WLRR95 pKa = 11.84DD96 pKa = 3.01SDD98 pKa = 3.62ITSARR103 pKa = 11.84PPCLPPAPTSLDD115 pKa = 3.47FPLRR119 pKa = 11.84GPLRR123 pKa = 11.84KK124 pKa = 9.5HH125 pKa = 5.62FRR127 pKa = 11.84NMLASRR133 pKa = 11.84TSSVRR138 pKa = 11.84AGTLFTGILQGVKK151 pKa = 9.93RR152 pKa = 11.84GCAPVPEE159 pKa = 4.34EE160 pKa = 5.12FEE162 pKa = 4.27VLSCQKK168 pKa = 10.29HH169 pKa = 5.29KK170 pKa = 10.82KK171 pKa = 10.06ALSQPLEE178 pKa = 4.18FSHH181 pKa = 7.49GPEE184 pKa = 4.22FFAKK188 pKa = 10.44FQAIWGKK195 pKa = 8.6TNTEE199 pKa = 3.43NRR201 pKa = 11.84RR202 pKa = 11.84AALHH206 pKa = 5.76SRR208 pKa = 11.84WKK210 pKa = 10.57RR211 pKa = 11.84VFLDD215 pKa = 3.82RR216 pKa = 11.84KK217 pKa = 7.39WARR220 pKa = 11.84HH221 pKa = 4.59LHH223 pKa = 5.47SASNHH228 pKa = 5.18ASVEE232 pKa = 4.14RR233 pKa = 11.84MRR235 pKa = 11.84SEE237 pKa = 4.04GGRR240 pKa = 11.84VAEE243 pKa = 4.09VFHH246 pKa = 6.33VNRR249 pKa = 11.84ILIGEE254 pKa = 4.28DD255 pKa = 3.13RR256 pKa = 11.84DD257 pKa = 3.75PRR259 pKa = 11.84LVDD262 pKa = 3.99EE263 pKa = 5.4PPLLDD268 pKa = 3.14MFEE271 pKa = 4.66RR272 pKa = 11.84NGRR275 pKa = 11.84VVEE278 pKa = 4.0RR279 pKa = 11.84RR280 pKa = 11.84GWPLASYY287 pKa = 10.37RR288 pKa = 11.84QFLRR292 pKa = 11.84QAAGMCRR299 pKa = 11.84GRR301 pKa = 11.84DD302 pKa = 3.12LRR304 pKa = 11.84ARR306 pKa = 11.84VEE308 pKa = 4.11LCLEE312 pKa = 4.03PLKK315 pKa = 11.02CRR317 pKa = 11.84VITKK321 pKa = 10.26GEE323 pKa = 4.07SVPYY327 pKa = 9.8FVAQTFQKK335 pKa = 10.96SMWKK339 pKa = 10.17ALQEE343 pKa = 3.93TGAFKK348 pKa = 9.95LTSCPVDD355 pKa = 3.13ASMLYY360 pKa = 10.64GIEE363 pKa = 5.22LSTKK367 pKa = 10.68DD368 pKa = 3.78LDD370 pKa = 4.34LPFDD374 pKa = 3.36QWVSGDD380 pKa = 3.54YY381 pKa = 10.74SAATDD386 pKa = 3.88GLTLEE391 pKa = 5.1VNQCCLRR398 pKa = 11.84AMLDD402 pKa = 3.48AFQATPEE409 pKa = 3.93EE410 pKa = 4.57RR411 pKa = 11.84EE412 pKa = 4.16VCSKK416 pKa = 11.09VLGCHH421 pKa = 4.83EE422 pKa = 3.96VSYY425 pKa = 10.69PDD427 pKa = 4.53RR428 pKa = 11.84LDD430 pKa = 3.78PEE432 pKa = 4.96ANGLEE437 pKa = 4.37PFHH440 pKa = 6.33MLNGQLMGSVLSFPVLCAVNLAAYY464 pKa = 7.54WCALEE469 pKa = 5.16EE470 pKa = 4.0YY471 pKa = 9.57TGRR474 pKa = 11.84KK475 pKa = 8.16FKK477 pKa = 10.96KK478 pKa = 8.37EE479 pKa = 3.8QLPVLVNGDD488 pKa = 4.35DD489 pKa = 3.95ILFKK493 pKa = 11.24ANAAFYY499 pKa = 9.0EE500 pKa = 4.68VWKK503 pKa = 10.24KK504 pKa = 6.7WVKK507 pKa = 10.22RR508 pKa = 11.84AGFSLSLGKK517 pKa = 10.4NYY519 pKa = 9.9ISPNFITVNSEE530 pKa = 3.46SWLHH534 pKa = 6.17KK535 pKa = 9.5GGSYY539 pKa = 9.82FRR541 pKa = 11.84KK542 pKa = 10.14LPFLNCGLLLQEE554 pKa = 4.42AAGPYY559 pKa = 9.19KK560 pKa = 10.78VPLRR564 pKa = 11.84AEE566 pKa = 3.87TAEE569 pKa = 4.15RR570 pKa = 11.84PLIPKK575 pKa = 7.98LQWIIDD581 pKa = 3.77NCNNPARR588 pKa = 11.84AFDD591 pKa = 4.64RR592 pKa = 11.84IKK594 pKa = 10.27HH595 pKa = 4.76HH596 pKa = 5.75WRR598 pKa = 11.84RR599 pKa = 11.84SINVHH604 pKa = 4.87TEE606 pKa = 4.04FGRR609 pKa = 11.84FNLCAPMEE617 pKa = 4.37LGGCGLKK624 pKa = 10.61LPEE627 pKa = 4.23AVRR630 pKa = 11.84PAVNFTAFQCLLAGRR645 pKa = 11.84SHH647 pKa = 6.45QSYY650 pKa = 11.11KK651 pKa = 10.72NLEE654 pKa = 4.05GTDD657 pKa = 2.95IRR659 pKa = 11.84DD660 pKa = 3.5RR661 pKa = 11.84PKK663 pKa = 10.49TGFEE667 pKa = 4.26RR668 pKa = 11.84ISVAEE673 pKa = 4.01VSKK676 pKa = 10.93SINPLPTEE684 pKa = 4.15SRR686 pKa = 11.84LGTAVLRR693 pKa = 11.84SPFEE697 pKa = 3.81PVRR700 pKa = 11.84GEE702 pKa = 3.94LEE704 pKa = 4.0VRR706 pKa = 11.84FEE708 pKa = 5.31DD709 pKa = 5.22PIACLRR715 pKa = 11.84VAGEE719 pKa = 4.38LNTAQTASDD728 pKa = 3.59QEE730 pKa = 4.23RR731 pKa = 11.84PVYY734 pKa = 9.61VLKK737 pKa = 10.74RR738 pKa = 11.84LSRR741 pKa = 11.84KK742 pKa = 9.47RR743 pKa = 11.84LGAVFSTGAKK753 pKa = 6.67ITKK756 pKa = 9.34PFLFPFEE763 pKa = 4.07VRR765 pKa = 11.84KK766 pKa = 9.6QLTRR770 pKa = 11.84GPTDD774 pKa = 3.77DD775 pKa = 4.7ASPPAMPEE783 pKa = 4.54DD784 pKa = 3.62IAEE787 pKa = 4.3MATEE791 pKa = 4.6LCKK794 pKa = 10.52SAPKK798 pKa = 9.65PSCGGGTSRR807 pKa = 11.84KK808 pKa = 6.6WW809 pKa = 3.06

Molecular weight: 91.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIK3|A0A1L3KIK3_9VIRU Uncharacterized protein OS=Beihai narna-like virus 9 OX=1922461 PE=4 SV=1
MM1 pKa = 7.75AKK3 pKa = 10.1QNGVRR8 pKa = 11.84RR9 pKa = 11.84TVQKK13 pKa = 10.32KK14 pKa = 8.32VKK16 pKa = 9.58SDD18 pKa = 2.93RR19 pKa = 11.84RR20 pKa = 11.84SRR22 pKa = 11.84ADD24 pKa = 3.18VVQAQGTGRR33 pKa = 11.84TVQRR37 pKa = 11.84AFAGTKK43 pKa = 9.71RR44 pKa = 11.84ATGLEE49 pKa = 4.02GWDD52 pKa = 3.52AFSPAHH58 pKa = 6.28LPLPRR63 pKa = 11.84SVGPYY68 pKa = 9.14TVTKK72 pKa = 7.44TTALISTNDD81 pKa = 3.92RR82 pKa = 11.84INIIGTFAEE91 pKa = 4.44TDD93 pKa = 3.39TTGTKK98 pKa = 9.87LWTNVGLVKK107 pKa = 10.44SVNSALPISDD117 pKa = 3.87PANTFLHH124 pKa = 6.73TIPFPGNNITGSGLSATPAAVSVQIMNPNPLQTSSGIVAAAVCPTQLDD172 pKa = 3.67LRR174 pKa = 11.84GRR176 pKa = 11.84TEE178 pKa = 3.37TWAEE182 pKa = 3.84FGTEE186 pKa = 4.91FISYY190 pKa = 7.4MRR192 pKa = 11.84PRR194 pKa = 11.84LMSAGKK200 pKa = 9.4LALRR204 pKa = 11.84GVQMDD209 pKa = 4.49SYY211 pKa = 8.68PLNMGALAEE220 pKa = 4.02FRR222 pKa = 11.84PVNGFGDD229 pKa = 3.93TTIQWDD235 pKa = 4.14SANPTHH241 pKa = 6.19PQGWAPIVIVNQASSEE257 pKa = 4.03DD258 pKa = 3.87PPLEE262 pKa = 4.34LNLLVTIEE270 pKa = 3.43WRR272 pKa = 11.84TRR274 pKa = 11.84FDD276 pKa = 2.88IGNPAVSSHH285 pKa = 5.01SHH287 pKa = 6.36HH288 pKa = 6.42GVSSDD293 pKa = 3.47QHH295 pKa = 5.31WDD297 pKa = 3.16RR298 pKa = 11.84LIRR301 pKa = 11.84AATNRR306 pKa = 11.84GAGAMDD312 pKa = 3.0IVEE315 pKa = 4.34RR316 pKa = 11.84VANAGTAAIGAYY328 pKa = 9.46NAARR332 pKa = 11.84PILNQLPMLANFF344 pKa = 4.48

Molecular weight: 36.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1153

344

809

576.5

64.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.714 ± 0.823

1.995 ± 0.864

4.163 ± 0.1

5.637 ± 1.384

4.683 ± 0.753

6.678 ± 0.741

2.082 ± 0.024

3.729 ± 0.909

4.683 ± 1.048

9.974 ± 1.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.995 ± 0.168

4.076 ± 1.028

6.852 ± 0.084

3.209 ± 0.436

7.892 ± 0.612

6.592 ± 0.195

5.637 ± 1.858

6.505 ± 0.239

2.255 ± 0.112

1.648 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski