Escherichia phage Lidtsur

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Bonnellvirus; Escherichia virus Lidtsur

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6DYZ1|A0A4D6DYZ1_9CAUD Uncharacterized protein OS=Escherichia phage Lidtsur OX=2562235 PE=4 SV=1
MM1 pKa = 7.42PRR3 pKa = 11.84ITKK6 pKa = 9.85VEE8 pKa = 4.03DD9 pKa = 3.31LKK11 pKa = 11.55EE12 pKa = 4.48GDD14 pKa = 3.98VYY16 pKa = 11.21QLSGGIFCAVVLDD29 pKa = 4.27TNKK32 pKa = 10.75SGFNYY37 pKa = 10.21VGLDD41 pKa = 3.54KK42 pKa = 10.99EE43 pKa = 4.49DD44 pKa = 4.36SNTLVFVGVDD54 pKa = 3.25NGQPQYY60 pKa = 10.81FPEE63 pKa = 4.98GLLDD67 pKa = 5.59DD68 pKa = 5.78PDD70 pKa = 4.0TEE72 pKa = 4.37YY73 pKa = 11.88GMVSKK78 pKa = 11.05AA79 pKa = 3.29

Molecular weight: 8.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6DYW1|A0A4D6DYW1_9CAUD Uncharacterized protein OS=Escherichia phage Lidtsur OX=2562235 PE=4 SV=1
MM1 pKa = 6.9NTNLLGWVKK10 pKa = 10.17RR11 pKa = 11.84VEE13 pKa = 3.94RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 5.54WEE18 pKa = 3.45TAIILKK24 pKa = 10.44VGISAGLAAAYY35 pKa = 7.35FLPTSAAIAVGISINLLWLWKK56 pKa = 10.39LL57 pKa = 3.44

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13211

33

1034

240.2

26.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.491 ± 0.522

1.029 ± 0.197

5.828 ± 0.256

6.109 ± 0.356

3.368 ± 0.199

7.789 ± 0.263

1.983 ± 0.231

4.708 ± 0.204

4.58 ± 0.359

8.137 ± 0.3

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.073 ± 0.243

4.156 ± 0.311

4.292 ± 0.313

4.905 ± 0.57

5.548 ± 0.284

5.73 ± 0.403

6.063 ± 0.397

7.138 ± 0.28

1.393 ± 0.114

3.679 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski