Enterobacteria phage P4 (Bacteriophage P4)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; unclassified Caudovirales

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P13058|GOP_BPP4 Protein gop OS=Enterobacteria phage P4 OX=10680 GN=gop PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.34VYY4 pKa = 10.58FEE6 pKa = 4.53NYY8 pKa = 9.09SYY10 pKa = 11.56YY11 pKa = 9.67PALRR15 pKa = 11.84TRR17 pKa = 11.84SAEE20 pKa = 3.8MTGLNNLSYY29 pKa = 10.69EE30 pKa = 4.29NKK32 pKa = 10.29KK33 pKa = 10.67KK34 pKa = 10.27ILPLISLGKK43 pKa = 8.45WPRR46 pKa = 11.84SEE48 pKa = 4.55EE49 pKa = 3.98IQVSLDD55 pKa = 3.03KK56 pKa = 11.17SLEE59 pKa = 4.13VMSNLPFILDD69 pKa = 3.51VTKK72 pKa = 10.99DD73 pKa = 3.75NSHH76 pKa = 6.45HH77 pKa = 6.31CASSFEE83 pKa = 4.26LLSPEE88 pKa = 4.31NGFKK92 pKa = 10.6NWIEE96 pKa = 3.9FCSRR100 pKa = 11.84NDD102 pKa = 3.45NIIPVVQMPDD112 pKa = 3.08SAKK115 pKa = 10.63LRR117 pKa = 11.84DD118 pKa = 3.47ISIQARR124 pKa = 11.84VLEE127 pKa = 4.13EE128 pKa = 4.16LKK130 pKa = 11.0GSIAFRR136 pKa = 11.84IRR138 pKa = 11.84NLNTDD143 pKa = 2.94INKK146 pKa = 8.8TLTSLVSMNSPEE158 pKa = 3.94NAIVFIDD165 pKa = 3.68LGYY168 pKa = 10.41IRR170 pKa = 11.84GNVSAITAAAINSINQIRR188 pKa = 11.84TEE190 pKa = 3.82IPEE193 pKa = 4.49AIISVLATSFPSSVTNFCRR212 pKa = 11.84EE213 pKa = 3.99NGQSGYY219 pKa = 10.0IDD221 pKa = 3.54VIEE224 pKa = 5.2RR225 pKa = 11.84EE226 pKa = 4.08LHH228 pKa = 5.16QNIGGSDD235 pKa = 3.27VAIYY239 pKa = 10.36GDD241 pKa = 3.67HH242 pKa = 6.64GSIHH246 pKa = 6.19SVVYY250 pKa = 10.58DD251 pKa = 4.02NIIGRR256 pKa = 11.84YY257 pKa = 7.12VPRR260 pKa = 11.84IDD262 pKa = 4.55IALNDD267 pKa = 2.73SWYY270 pKa = 10.04FEE272 pKa = 4.64RR273 pKa = 11.84RR274 pKa = 11.84PGMNKK279 pKa = 9.95EE280 pKa = 4.11GFIEE284 pKa = 4.17AAKK287 pKa = 10.47SILAEE292 pKa = 3.94YY293 pKa = 9.46PHH295 pKa = 5.85YY296 pKa = 10.37QRR298 pKa = 11.84EE299 pKa = 4.36DD300 pKa = 2.88SWGAAMIRR308 pKa = 11.84NAAIGDD314 pKa = 3.78IAGMGSPAKK323 pKa = 9.75WIAVRR328 pKa = 11.84VNLHH332 pKa = 6.1LNKK335 pKa = 10.23QIEE338 pKa = 4.33LSEE341 pKa = 4.01ALQYY345 pKa = 11.48GFDD348 pKa = 4.07HH349 pKa = 7.62DD350 pKa = 4.69EE351 pKa = 4.06EE352 pKa = 5.18DD353 pKa = 5.07LII355 pKa = 6.09

Molecular weight: 39.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P10277|PRIM_BPP4 Putative P4-specific DNA primase OS=Enterobacteria phage P4 OX=10680 GN=Alpha PE=1 SV=1
MM1 pKa = 7.52CPQMKK6 pKa = 10.39LNARR10 pKa = 11.84QVEE13 pKa = 4.31TAKK16 pKa = 10.91PKK18 pKa = 10.79DD19 pKa = 3.61KK20 pKa = 10.0TYY22 pKa = 11.59KK23 pKa = 9.33MADD26 pKa = 2.99GGGLYY31 pKa = 10.65LEE33 pKa = 5.0VSAKK37 pKa = 9.92GSKK40 pKa = 7.48YY41 pKa = 8.13WRR43 pKa = 11.84MKK45 pKa = 9.5YY46 pKa = 9.87RR47 pKa = 11.84RR48 pKa = 11.84PSDD51 pKa = 3.35KK52 pKa = 11.12KK53 pKa = 10.01EE54 pKa = 3.59DD55 pKa = 3.53RR56 pKa = 11.84LAFGVWPTVTLAQARR71 pKa = 11.84AKK73 pKa = 10.29RR74 pKa = 11.84DD75 pKa = 3.4EE76 pKa = 4.15AKK78 pKa = 10.53KK79 pKa = 10.84LLVQGIDD86 pKa = 3.19PKK88 pKa = 11.05VVQKK92 pKa = 9.3EE93 pKa = 3.86ARR95 pKa = 11.84AEE97 pKa = 3.8NSGAYY102 pKa = 8.54TFEE105 pKa = 5.91AIARR109 pKa = 11.84EE110 pKa = 3.81WHH112 pKa = 6.32ASNKK116 pKa = 9.2RR117 pKa = 11.84WSEE120 pKa = 3.87DD121 pKa = 2.76HH122 pKa = 6.69RR123 pKa = 11.84SRR125 pKa = 11.84VLRR128 pKa = 11.84YY129 pKa = 9.34LEE131 pKa = 5.45LYY133 pKa = 9.64IFPHH137 pKa = 6.58IGSSDD142 pKa = 2.76IRR144 pKa = 11.84QLKK147 pKa = 8.56TSHH150 pKa = 6.75LLAPIKK156 pKa = 10.64KK157 pKa = 8.48VDD159 pKa = 3.32ASGKK163 pKa = 9.46HH164 pKa = 6.14DD165 pKa = 3.36VAQRR169 pKa = 11.84LQQRR173 pKa = 11.84VTAIMRR179 pKa = 11.84YY180 pKa = 9.16AVQNDD185 pKa = 4.19YY186 pKa = 11.1IDD188 pKa = 4.69SNPASDD194 pKa = 3.41MAGALSTTKK203 pKa = 10.29ARR205 pKa = 11.84HH206 pKa = 5.04YY207 pKa = 9.05PALPSSRR214 pKa = 11.84FPEE217 pKa = 4.32FLARR221 pKa = 11.84LAAYY225 pKa = 9.53RR226 pKa = 11.84GRR228 pKa = 11.84VMTRR232 pKa = 11.84IAVKK236 pKa = 10.53LSLLTFVRR244 pKa = 11.84SSEE247 pKa = 3.82LRR249 pKa = 11.84FARR252 pKa = 11.84WDD254 pKa = 3.31EE255 pKa = 3.85FDD257 pKa = 3.71FDD259 pKa = 3.92KK260 pKa = 11.33SLWRR264 pKa = 11.84IPAKK268 pKa = 10.3RR269 pKa = 11.84EE270 pKa = 3.69EE271 pKa = 4.4IKK273 pKa = 10.66GVRR276 pKa = 11.84YY277 pKa = 9.94SYY279 pKa = 11.08RR280 pKa = 11.84GMKK283 pKa = 9.05MKK285 pKa = 10.39EE286 pKa = 3.7EE287 pKa = 4.71HH288 pKa = 6.46IVPLSRR294 pKa = 11.84QAMILLNQLKK304 pKa = 10.09QISGDD309 pKa = 3.64KK310 pKa = 10.47EE311 pKa = 3.96LLFPGDD317 pKa = 3.48HH318 pKa = 7.44DD319 pKa = 3.78ATKK322 pKa = 10.89VMSEE326 pKa = 3.95NTVNSALRR334 pKa = 11.84AMGYY338 pKa = 7.27DD339 pKa = 3.31TKK341 pKa = 11.12TEE343 pKa = 4.1VCGHH347 pKa = 6.36GFRR350 pKa = 11.84TMARR354 pKa = 11.84GALGEE359 pKa = 4.26SGLWSDD365 pKa = 5.4DD366 pKa = 3.84AIEE369 pKa = 4.4RR370 pKa = 11.84QLSHH374 pKa = 6.38SEE376 pKa = 4.1RR377 pKa = 11.84NNVRR381 pKa = 11.84AAYY384 pKa = 9.32IHH386 pKa = 5.95TSEE389 pKa = 4.67HH390 pKa = 5.72LDD392 pKa = 3.33EE393 pKa = 5.28RR394 pKa = 11.84RR395 pKa = 11.84LMMQWWADD403 pKa = 3.63YY404 pKa = 11.6LDD406 pKa = 3.77MNRR409 pKa = 11.84NKK411 pKa = 10.38YY412 pKa = 9.13ISLMIIQNTKK422 pKa = 10.48KK423 pKa = 10.35YY424 pKa = 10.5LNKK427 pKa = 10.15NSYY430 pKa = 8.32WLIFKK435 pKa = 10.07MSVKK439 pKa = 10.53

Molecular weight: 50.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

3145

88

777

241.9

27.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.793 ± 0.945

1.494 ± 0.338

5.278 ± 0.472

6.455 ± 0.495

3.084 ± 0.354

5.723 ± 0.721

2.607 ± 0.316

5.564 ± 0.915

5.278 ± 0.662

9.539 ± 0.555

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.703 ± 0.301

4.102 ± 0.68

4.388 ± 0.696

3.879 ± 0.5

7.568 ± 0.562

7.154 ± 0.724

4.674 ± 0.605

6.041 ± 0.463

1.494 ± 0.194

3.18 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski