Bacillus phage Shanette

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Spounavirinae; Siminovitchvirus; Bacillus virus Shanette

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5MTJ8|S5MTJ8_9CAUD Uncharacterized protein OS=Bacillus phage Shanette OX=1296656 GN=SHANETTE_13 PE=4 SV=1
MM1 pKa = 8.63VYY3 pKa = 9.69MKK5 pKa = 10.46LLWTITYY12 pKa = 8.65TKK14 pKa = 10.73DD15 pKa = 2.97GLQLFLEE22 pKa = 4.74AEE24 pKa = 4.29NKK26 pKa = 9.84IEE28 pKa = 4.08AVNMIDD34 pKa = 3.74YY35 pKa = 10.04LVHH38 pKa = 6.62EE39 pKa = 5.19GVSLASITVFPHH51 pKa = 5.86CTGQPASEE59 pKa = 5.04FYY61 pKa = 11.41VEE63 pKa = 5.08VPDD66 pKa = 5.52LEE68 pKa = 5.02LGQYY72 pKa = 10.77CIMFTDD78 pKa = 4.6GDD80 pKa = 4.51PIIVDD85 pKa = 3.72SEE87 pKa = 4.58GVLVDD92 pKa = 4.9SDD94 pKa = 5.71DD95 pKa = 4.3PEE97 pKa = 5.19CPNTVDD103 pKa = 4.63GVWNKK108 pKa = 10.42LPGIATEE115 pKa = 4.0WASVKK120 pKa = 10.02EE121 pKa = 4.03QVDD124 pKa = 3.37AGVDD128 pKa = 4.12FGTGYY133 pKa = 11.21YY134 pKa = 10.18KK135 pKa = 10.98LEE137 pKa = 4.03VDD139 pKa = 3.76NQISGTIQRR148 pKa = 11.84IVRR151 pKa = 11.84GTLL154 pKa = 2.99

Molecular weight: 17.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5M5A5|S5M5A5_9CAUD Uncharacterized protein OS=Bacillus phage Shanette OX=1296656 GN=SHANETTE_217 PE=4 SV=1
MM1 pKa = 8.16DD2 pKa = 5.48KK3 pKa = 9.97NTMGGLARR11 pKa = 11.84ALRR14 pKa = 11.84NLHH17 pKa = 6.12YY18 pKa = 10.36YY19 pKa = 9.09EE20 pKa = 4.37KK21 pKa = 10.46SGRR24 pKa = 11.84NPVLSPFSLIRR35 pKa = 11.84RR36 pKa = 11.84LFIGG40 pKa = 3.76

Molecular weight: 4.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

220

0

220

43013

21

3592

195.5

22.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.056 ± 0.268

1.021 ± 0.072

6.459 ± 0.161

7.681 ± 0.341

3.432 ± 0.1

7.082 ± 0.258

2.26 ± 0.18

6.328 ± 0.164

8.109 ± 0.243

7.87 ± 0.193

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.248 ± 0.158

4.843 ± 0.19

3.571 ± 0.137

3.364 ± 0.117

4.494 ± 0.151

5.84 ± 0.178

6.019 ± 0.308

6.965 ± 0.173

1.418 ± 0.073

3.941 ± 0.103
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski