Nitrososphaera viennensis EN76

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Nitrososphaeria; Nitrososphaerales; Nitrososphaeraceae; Nitrososphaera; Nitrososphaera viennensis

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3117 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A060HIF4|A0A060HIF4_9ARCH Uncharacterized protein OS=Nitrososphaera viennensis EN76 OX=926571 GN=NVIE_010850 PE=4 SV=1
MM1 pKa = 8.01SSLTGNKK8 pKa = 9.39LAFLLAVLAVGFAIASSVTAVPAAFAGGNDD38 pKa = 4.7DD39 pKa = 6.27DD40 pKa = 6.17DD41 pKa = 4.26NHH43 pKa = 7.19DD44 pKa = 4.11DD45 pKa = 3.35KK46 pKa = 11.79YY47 pKa = 11.41YY48 pKa = 10.94YY49 pKa = 10.48NNHH52 pKa = 6.59DD53 pKa = 4.96HH54 pKa = 7.84DD55 pKa = 6.85DD56 pKa = 4.76DD57 pKa = 7.62DD58 pKa = 7.69DD59 pKa = 7.21DD60 pKa = 7.68DD61 pKa = 7.5DD62 pKa = 6.74DD63 pKa = 6.27DD64 pKa = 6.0KK65 pKa = 11.97CDD67 pKa = 5.5DD68 pKa = 5.36DD69 pKa = 5.62NHH71 pKa = 7.79DD72 pKa = 4.36DD73 pKa = 4.94DD74 pKa = 6.04KK75 pKa = 12.06NNNYY79 pKa = 10.04DD80 pKa = 4.56DD81 pKa = 4.69KK82 pKa = 11.99NNDD85 pKa = 3.07NHH87 pKa = 7.77DD88 pKa = 3.83DD89 pKa = 4.06DD90 pKa = 5.82KK91 pKa = 12.05NNNYY95 pKa = 9.97DD96 pKa = 4.13DD97 pKa = 5.43KK98 pKa = 11.64NNCYY102 pKa = 10.34CDD104 pKa = 5.86DD105 pKa = 5.9DD106 pKa = 7.43DD107 pKa = 6.65DD108 pKa = 6.2DD109 pKa = 6.82KK110 pKa = 12.17NDD112 pKa = 3.9DD113 pKa = 4.58DD114 pKa = 5.71KK115 pKa = 12.05NDD117 pKa = 3.74YY118 pKa = 10.59HH119 pKa = 7.87SSSYY123 pKa = 10.03NGNWEE128 pKa = 3.98NYY130 pKa = 9.94KK131 pKa = 9.15YY132 pKa = 9.51TSYY135 pKa = 11.78NNDD138 pKa = 3.42DD139 pKa = 4.82HH140 pKa = 8.76DD141 pKa = 7.08DD142 pKa = 4.77DD143 pKa = 7.6DD144 pKa = 7.59DD145 pKa = 7.58DD146 pKa = 7.73DD147 pKa = 6.47DD148 pKa = 6.08DD149 pKa = 6.22KK150 pKa = 12.0CDD152 pKa = 5.66DD153 pKa = 5.31DD154 pKa = 6.17DD155 pKa = 5.13NHH157 pKa = 7.09DD158 pKa = 3.04NHH160 pKa = 6.82YY161 pKa = 11.14NHH163 pKa = 7.74DD164 pKa = 3.89YY165 pKa = 9.23WKK167 pKa = 10.8KK168 pKa = 10.41DD169 pKa = 3.03

Molecular weight: 19.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A060HQ96|A0A060HQ96_9ARCH Uncharacterized protein OS=Nitrososphaera viennensis EN76 OX=926571 GN=NVIE_030130 PE=4 SV=1
MM1 pKa = 7.82AARR4 pKa = 11.84KK5 pKa = 7.3NTSRR9 pKa = 11.84KK10 pKa = 8.62KK11 pKa = 10.4RR12 pKa = 11.84LMKK15 pKa = 9.54KK16 pKa = 7.64TRR18 pKa = 11.84QNQPVPAWVIIRR30 pKa = 11.84TSRR33 pKa = 11.84KK34 pKa = 9.14VRR36 pKa = 11.84TNPKK40 pKa = 8.11RR41 pKa = 11.84RR42 pKa = 11.84LWRR45 pKa = 11.84RR46 pKa = 11.84SDD48 pKa = 3.29VRR50 pKa = 11.84VGG52 pKa = 3.15

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3117

0

3117

729208

20

4059

233.9

25.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.406 ± 0.068

0.985 ± 0.018

5.501 ± 0.038

6.208 ± 0.052

3.868 ± 0.036

7.484 ± 0.048

1.886 ± 0.023

6.422 ± 0.045

6.484 ± 0.051

8.57 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.941 ± 0.029

3.679 ± 0.036

4.113 ± 0.027

3.188 ± 0.034

5.384 ± 0.045

6.79 ± 0.072

5.29 ± 0.067

7.613 ± 0.044

0.99 ± 0.017

3.199 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski