Amino acid dipepetide frequency for Bacillus phage vB_BtS_BMBtp3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.387AlaAla: 5.387 ± 1.315
0.471AlaCys: 0.471 ± 0.193
3.703AlaAsp: 3.703 ± 0.504
5.118AlaGlu: 5.118 ± 0.58
2.155AlaPhe: 2.155 ± 0.379
3.299AlaGly: 3.299 ± 0.554
0.808AlaHis: 0.808 ± 0.216
4.309AlaIle: 4.309 ± 0.709
5.656AlaLys: 5.656 ± 0.487
4.848AlaLeu: 4.848 ± 0.812
1.683AlaMet: 1.683 ± 0.388
3.367AlaAsn: 3.367 ± 0.569
1.347AlaPro: 1.347 ± 0.359
2.491AlaGln: 2.491 ± 0.394
3.232AlaArg: 3.232 ± 0.438
2.895AlaSer: 2.895 ± 0.533
3.367AlaThr: 3.367 ± 0.563
3.905AlaVal: 3.905 ± 0.572
1.077AlaTrp: 1.077 ± 0.279
2.222AlaTyr: 2.222 ± 0.466
0.0AlaXaa: 0.0 ± 0.0
Cys
0.539CysAla: 0.539 ± 0.186
0.067CysCys: 0.067 ± 0.069
0.943CysAsp: 0.943 ± 0.255
1.077CysGlu: 1.077 ± 0.261
0.539CysPhe: 0.539 ± 0.191
0.337CysGly: 0.337 ± 0.151
0.202CysHis: 0.202 ± 0.122
0.471CysIle: 0.471 ± 0.155
0.808CysLys: 0.808 ± 0.241
0.404CysLeu: 0.404 ± 0.173
0.269CysMet: 0.269 ± 0.119
0.404CysAsn: 0.404 ± 0.161
0.0CysPro: 0.0 ± 0.0
0.269CysGln: 0.269 ± 0.116
0.404CysArg: 0.404 ± 0.185
0.135CysSer: 0.135 ± 0.092
0.404CysThr: 0.404 ± 0.129
0.539CysVal: 0.539 ± 0.205
0.067CysTrp: 0.067 ± 0.061
0.202CysTyr: 0.202 ± 0.143
0.0CysXaa: 0.0 ± 0.0
Asp
3.636AspAla: 3.636 ± 0.633
0.135AspCys: 0.135 ± 0.092
3.434AspAsp: 3.434 ± 0.625
4.309AspGlu: 4.309 ± 0.717
2.963AspPhe: 2.963 ± 0.426
3.905AspGly: 3.905 ± 0.456
1.01AspHis: 1.01 ± 0.267
5.05AspIle: 5.05 ± 0.57
5.118AspLys: 5.118 ± 0.589
4.983AspLeu: 4.983 ± 0.578
2.289AspMet: 2.289 ± 0.408
2.828AspAsn: 2.828 ± 0.478
2.155AspPro: 2.155 ± 0.344
2.02AspGln: 2.02 ± 0.343
2.761AspArg: 2.761 ± 0.425
2.626AspSer: 2.626 ± 0.393
3.097AspThr: 3.097 ± 0.39
4.309AspVal: 4.309 ± 0.559
1.077AspTrp: 1.077 ± 0.258
2.761AspTyr: 2.761 ± 0.426
0.0AspXaa: 0.0 ± 0.0
Glu
5.589GluAla: 5.589 ± 0.64
0.539GluCys: 0.539 ± 0.184
4.511GluAsp: 4.511 ± 0.686
6.532GluGlu: 6.532 ± 0.909
3.097GluPhe: 3.097 ± 0.455
4.377GluGly: 4.377 ± 0.436
1.077GluHis: 1.077 ± 0.31
6.195GluIle: 6.195 ± 0.784
8.417GluLys: 8.417 ± 0.72
7.676GluLeu: 7.676 ± 0.805
2.222GluMet: 2.222 ± 0.543
3.973GluAsn: 3.973 ± 0.47
2.222GluPro: 2.222 ± 0.491
4.377GluGln: 4.377 ± 0.652
4.511GluArg: 4.511 ± 0.655
4.579GluSer: 4.579 ± 0.678
3.905GluThr: 3.905 ± 0.645
4.848GluVal: 4.848 ± 0.628
1.549GluTrp: 1.549 ± 0.305
2.357GluTyr: 2.357 ± 0.425
0.0GluXaa: 0.0 ± 0.0
Phe
2.693PheAla: 2.693 ± 0.418
0.337PheCys: 0.337 ± 0.139
3.097PheAsp: 3.097 ± 0.442
2.895PheGlu: 2.895 ± 0.403
1.212PhePhe: 1.212 ± 0.32
3.434PheGly: 3.434 ± 0.529
0.741PheHis: 0.741 ± 0.192
4.04PheIle: 4.04 ± 0.498
3.703PheLys: 3.703 ± 0.566
2.761PheLeu: 2.761 ± 0.421
1.01PheMet: 1.01 ± 0.208
2.357PheAsn: 2.357 ± 0.421
1.077PhePro: 1.077 ± 0.301
1.414PheGln: 1.414 ± 0.324
1.751PheArg: 1.751 ± 0.444
2.491PheSer: 2.491 ± 0.453
1.549PheThr: 1.549 ± 0.359
2.424PheVal: 2.424 ± 0.39
0.539PheTrp: 0.539 ± 0.211
1.01PheTyr: 1.01 ± 0.277
0.0PheXaa: 0.0 ± 0.0
Gly
3.569GlyAla: 3.569 ± 0.765
0.337GlyCys: 0.337 ± 0.183
3.636GlyAsp: 3.636 ± 0.427
4.511GlyGlu: 4.511 ± 0.481
2.693GlyPhe: 2.693 ± 0.382
3.434GlyGly: 3.434 ± 0.486
0.741GlyHis: 0.741 ± 0.226
3.367GlyIle: 3.367 ± 0.685
5.454GlyLys: 5.454 ± 0.615
5.656GlyLeu: 5.656 ± 0.743
1.683GlyMet: 1.683 ± 0.325
2.828GlyAsn: 2.828 ± 0.46
0.741GlyPro: 0.741 ± 0.209
1.818GlyGln: 1.818 ± 0.354
3.03GlyArg: 3.03 ± 0.406
3.097GlySer: 3.097 ± 0.567
3.367GlyThr: 3.367 ± 0.466
4.04GlyVal: 4.04 ± 0.744
1.683GlyTrp: 1.683 ± 0.328
3.434GlyTyr: 3.434 ± 0.511
0.0GlyXaa: 0.0 ± 0.0
His
0.741HisAla: 0.741 ± 0.25
0.202HisCys: 0.202 ± 0.151
1.279HisAsp: 1.279 ± 0.268
0.673HisGlu: 0.673 ± 0.215
0.606HisPhe: 0.606 ± 0.191
1.077HisGly: 1.077 ± 0.255
0.337HisHis: 0.337 ± 0.154
1.279HisIle: 1.279 ± 0.277
0.943HisLys: 0.943 ± 0.304
1.145HisLeu: 1.145 ± 0.245
0.202HisMet: 0.202 ± 0.115
0.471HisAsn: 0.471 ± 0.254
0.404HisPro: 0.404 ± 0.186
0.741HisGln: 0.741 ± 0.22
0.808HisArg: 0.808 ± 0.235
1.279HisSer: 1.279 ± 0.314
1.279HisThr: 1.279 ± 0.335
1.347HisVal: 1.347 ± 0.308
0.471HisTrp: 0.471 ± 0.18
1.279HisTyr: 1.279 ± 0.264
0.0HisXaa: 0.0 ± 0.0
Ile
4.915IleAla: 4.915 ± 0.682
0.943IleCys: 0.943 ± 0.26
4.579IleAsp: 4.579 ± 0.617
6.868IleGlu: 6.868 ± 0.485
2.357IlePhe: 2.357 ± 0.38
4.04IleGly: 4.04 ± 0.47
1.212IleHis: 1.212 ± 0.328
4.848IleIle: 4.848 ± 0.622
5.993IleLys: 5.993 ± 0.692
5.387IleLeu: 5.387 ± 0.597
1.481IleMet: 1.481 ± 0.326
5.387IleAsn: 5.387 ± 0.837
2.491IlePro: 2.491 ± 0.387
2.761IleGln: 2.761 ± 0.487
3.097IleArg: 3.097 ± 0.513
4.579IleSer: 4.579 ± 0.625
4.511IleThr: 4.511 ± 0.497
4.04IleVal: 4.04 ± 0.48
1.01IleTrp: 1.01 ± 0.188
2.693IleTyr: 2.693 ± 0.551
0.0IleXaa: 0.0 ± 0.0
Lys
4.511LysAla: 4.511 ± 0.69
0.404LysCys: 0.404 ± 0.199
5.522LysAsp: 5.522 ± 0.674
9.158LysGlu: 9.158 ± 0.802
2.828LysPhe: 2.828 ± 0.468
4.579LysGly: 4.579 ± 0.614
1.549LysHis: 1.549 ± 0.227
6.734LysIle: 6.734 ± 0.693
8.619LysLys: 8.619 ± 1.169
7.07LysLeu: 7.07 ± 0.7
2.895LysMet: 2.895 ± 0.497
4.309LysAsn: 4.309 ± 0.556
2.761LysPro: 2.761 ± 0.403
4.511LysGln: 4.511 ± 0.622
6.262LysArg: 6.262 ± 0.77
5.118LysSer: 5.118 ± 0.569
4.848LysThr: 4.848 ± 0.722
6.262LysVal: 6.262 ± 0.646
0.741LysTrp: 0.741 ± 0.239
3.771LysTyr: 3.771 ± 0.578
0.0LysXaa: 0.0 ± 0.0
Leu
4.646LeuAla: 4.646 ± 0.617
0.606LeuCys: 0.606 ± 0.216
4.983LeuAsp: 4.983 ± 0.578
7.003LeuGlu: 7.003 ± 0.635
3.367LeuPhe: 3.367 ± 0.466
4.915LeuGly: 4.915 ± 0.85
1.414LeuHis: 1.414 ± 0.375
5.522LeuIle: 5.522 ± 0.563
6.734LeuLys: 6.734 ± 0.817
5.454LeuLeu: 5.454 ± 0.812
1.818LeuMet: 1.818 ± 0.312
4.242LeuAsn: 4.242 ± 0.515
2.357LeuPro: 2.357 ± 0.446
4.579LeuGln: 4.579 ± 0.844
4.377LeuArg: 4.377 ± 0.612
5.05LeuSer: 5.05 ± 0.561
4.444LeuThr: 4.444 ± 0.562
5.454LeuVal: 5.454 ± 0.575
0.741LeuTrp: 0.741 ± 0.231
3.367LeuTyr: 3.367 ± 0.474
0.0LeuXaa: 0.0 ± 0.0
Met
2.222MetAla: 2.222 ± 0.43
0.135MetCys: 0.135 ± 0.1
1.145MetAsp: 1.145 ± 0.29
2.357MetGlu: 2.357 ± 0.389
1.077MetPhe: 1.077 ± 0.342
0.943MetGly: 0.943 ± 0.23
0.471MetHis: 0.471 ± 0.195
2.02MetIle: 2.02 ± 0.37
3.367MetLys: 3.367 ± 0.523
2.289MetLeu: 2.289 ± 0.444
0.875MetMet: 0.875 ± 0.255
1.885MetAsn: 1.885 ± 0.422
0.943MetPro: 0.943 ± 0.268
1.549MetGln: 1.549 ± 0.332
0.875MetArg: 0.875 ± 0.229
1.953MetSer: 1.953 ± 0.323
2.02MetThr: 2.02 ± 0.295
0.943MetVal: 0.943 ± 0.285
0.404MetTrp: 0.404 ± 0.147
0.741MetTyr: 0.741 ± 0.235
0.0MetXaa: 0.0 ± 0.0
Asn
3.097AsnAla: 3.097 ± 0.538
0.606AsnCys: 0.606 ± 0.206
2.895AsnAsp: 2.895 ± 0.513
4.579AsnGlu: 4.579 ± 0.674
1.279AsnPhe: 1.279 ± 0.301
4.04AsnGly: 4.04 ± 0.78
1.01AsnHis: 1.01 ± 0.258
3.703AsnIle: 3.703 ± 0.452
4.915AsnLys: 4.915 ± 0.538
4.579AsnLeu: 4.579 ± 0.596
1.616AsnMet: 1.616 ± 0.337
2.289AsnAsn: 2.289 ± 0.37
1.818AsnPro: 1.818 ± 0.295
1.818AsnGln: 1.818 ± 0.365
2.289AsnArg: 2.289 ± 0.425
2.222AsnSer: 2.222 ± 0.408
2.963AsnThr: 2.963 ± 0.519
3.165AsnVal: 3.165 ± 0.474
0.808AsnTrp: 0.808 ± 0.248
1.414AsnTyr: 1.414 ± 0.329
0.0AsnXaa: 0.0 ± 0.0
Pro
1.683ProAla: 1.683 ± 0.3
0.135ProCys: 0.135 ± 0.093
1.481ProAsp: 1.481 ± 0.324
2.155ProGlu: 2.155 ± 0.394
0.808ProPhe: 0.808 ± 0.248
1.414ProGly: 1.414 ± 0.292
0.673ProHis: 0.673 ± 0.23
1.751ProIle: 1.751 ± 0.333
2.761ProLys: 2.761 ± 0.355
2.424ProLeu: 2.424 ± 0.478
0.673ProMet: 0.673 ± 0.185
1.549ProAsn: 1.549 ± 0.379
0.875ProPro: 0.875 ± 0.262
1.077ProGln: 1.077 ± 0.288
0.673ProArg: 0.673 ± 0.247
2.087ProSer: 2.087 ± 0.389
1.751ProThr: 1.751 ± 0.383
2.491ProVal: 2.491 ± 0.518
0.337ProTrp: 0.337 ± 0.151
1.279ProTyr: 1.279 ± 0.334
0.0ProXaa: 0.0 ± 0.0
Gln
2.828GlnAla: 2.828 ± 0.387
0.202GlnCys: 0.202 ± 0.139
2.02GlnAsp: 2.02 ± 0.374
4.175GlnGlu: 4.175 ± 0.48
2.424GlnPhe: 2.424 ± 0.36
2.491GlnGly: 2.491 ± 0.455
0.539GlnHis: 0.539 ± 0.178
3.501GlnIle: 3.501 ± 0.346
3.973GlnLys: 3.973 ± 0.428
4.107GlnLeu: 4.107 ± 0.876
1.212GlnMet: 1.212 ± 0.325
1.683GlnAsn: 1.683 ± 0.352
1.279GlnPro: 1.279 ± 0.304
2.357GlnGln: 2.357 ± 0.483
2.357GlnArg: 2.357 ± 0.295
1.885GlnSer: 1.885 ± 0.366
1.885GlnThr: 1.885 ± 0.398
2.289GlnVal: 2.289 ± 0.414
0.404GlnTrp: 0.404 ± 0.145
1.616GlnTyr: 1.616 ± 0.256
0.0GlnXaa: 0.0 ± 0.0
Arg
3.232ArgAla: 3.232 ± 0.552
0.606ArgCys: 0.606 ± 0.206
2.491ArgAsp: 2.491 ± 0.371
2.693ArgGlu: 2.693 ± 0.414
2.761ArgPhe: 2.761 ± 0.416
3.367ArgGly: 3.367 ± 0.443
1.01ArgHis: 1.01 ± 0.277
4.242ArgIle: 4.242 ± 0.56
5.118ArgLys: 5.118 ± 0.755
4.646ArgLeu: 4.646 ± 0.621
1.549ArgMet: 1.549 ± 0.241
1.683ArgAsn: 1.683 ± 0.29
0.875ArgPro: 0.875 ± 0.248
1.481ArgGln: 1.481 ± 0.277
2.087ArgArg: 2.087 ± 0.454
2.693ArgSer: 2.693 ± 0.46
2.222ArgThr: 2.222 ± 0.375
2.357ArgVal: 2.357 ± 0.487
0.404ArgTrp: 0.404 ± 0.2
1.953ArgTyr: 1.953 ± 0.452
0.0ArgXaa: 0.0 ± 0.0
Ser
3.299SerAla: 3.299 ± 0.478
0.337SerCys: 0.337 ± 0.161
3.771SerAsp: 3.771 ± 0.452
4.713SerGlu: 4.713 ± 0.474
2.895SerPhe: 2.895 ± 0.425
2.895SerGly: 2.895 ± 0.47
0.471SerHis: 0.471 ± 0.182
4.309SerIle: 4.309 ± 0.507
5.791SerLys: 5.791 ± 0.65
4.444SerLeu: 4.444 ± 0.586
1.549SerMet: 1.549 ± 0.276
2.491SerAsn: 2.491 ± 0.374
1.212SerPro: 1.212 ± 0.26
2.559SerGln: 2.559 ± 0.385
2.02SerArg: 2.02 ± 0.371
3.232SerSer: 3.232 ± 0.568
3.232SerThr: 3.232 ± 0.508
4.107SerVal: 4.107 ± 0.556
0.337SerTrp: 0.337 ± 0.138
1.953SerTyr: 1.953 ± 0.412
0.0SerXaa: 0.0 ± 0.0
Thr
3.232ThrAla: 3.232 ± 0.429
0.269ThrCys: 0.269 ± 0.165
3.03ThrAsp: 3.03 ± 0.565
3.905ThrGlu: 3.905 ± 0.574
3.232ThrPhe: 3.232 ± 0.431
3.232ThrGly: 3.232 ± 0.555
1.347ThrHis: 1.347 ± 0.314
3.973ThrIle: 3.973 ± 0.466
5.791ThrLys: 5.791 ± 0.798
4.444ThrLeu: 4.444 ± 0.692
1.414ThrMet: 1.414 ± 0.316
3.165ThrAsn: 3.165 ± 0.468
2.491ThrPro: 2.491 ± 0.469
2.02ThrGln: 2.02 ± 0.358
1.818ThrArg: 1.818 ± 0.392
2.693ThrSer: 2.693 ± 0.448
2.895ThrThr: 2.895 ± 0.451
3.905ThrVal: 3.905 ± 0.646
0.606ThrTrp: 0.606 ± 0.158
2.289ThrTyr: 2.289 ± 0.536
0.0ThrXaa: 0.0 ± 0.0
Val
2.828ValAla: 2.828 ± 0.456
1.01ValCys: 1.01 ± 0.306
4.579ValAsp: 4.579 ± 0.697
5.185ValGlu: 5.185 ± 0.689
2.087ValPhe: 2.087 ± 0.419
3.905ValGly: 3.905 ± 0.546
1.279ValHis: 1.279 ± 0.32
4.309ValIle: 4.309 ± 0.577
5.05ValLys: 5.05 ± 0.543
4.309ValLeu: 4.309 ± 0.537
2.155ValMet: 2.155 ± 0.39
3.771ValAsn: 3.771 ± 0.475
2.087ValPro: 2.087 ± 0.309
3.434ValGln: 3.434 ± 0.364
2.424ValArg: 2.424 ± 0.477
4.309ValSer: 4.309 ± 0.525
5.05ValThr: 5.05 ± 0.62
3.771ValVal: 3.771 ± 0.505
0.808ValTrp: 0.808 ± 0.219
1.414ValTyr: 1.414 ± 0.325
0.0ValXaa: 0.0 ± 0.0
Trp
0.741TrpAla: 0.741 ± 0.201
0.337TrpCys: 0.337 ± 0.158
1.279TrpAsp: 1.279 ± 0.347
1.549TrpGlu: 1.549 ± 0.287
0.269TrpPhe: 0.269 ± 0.14
0.943TrpGly: 0.943 ± 0.234
0.202TrpHis: 0.202 ± 0.146
1.01TrpIle: 1.01 ± 0.298
1.414TrpLys: 1.414 ± 0.37
0.875TrpLeu: 0.875 ± 0.216
0.606TrpMet: 0.606 ± 0.227
0.875TrpAsn: 0.875 ± 0.242
0.067TrpPro: 0.067 ± 0.076
0.471TrpGln: 0.471 ± 0.168
0.808TrpArg: 0.808 ± 0.272
0.875TrpSer: 0.875 ± 0.196
0.875TrpThr: 0.875 ± 0.225
0.471TrpVal: 0.471 ± 0.188
0.067TrpTrp: 0.067 ± 0.065
0.135TrpTyr: 0.135 ± 0.086
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.953TyrAla: 1.953 ± 0.345
0.404TyrCys: 0.404 ± 0.186
2.087TyrAsp: 2.087 ± 0.403
3.097TyrGlu: 3.097 ± 0.408
1.885TyrPhe: 1.885 ± 0.34
2.491TyrGly: 2.491 ± 0.399
0.471TyrHis: 0.471 ± 0.185
2.559TyrIle: 2.559 ± 0.391
2.828TyrLys: 2.828 ± 0.491
3.636TyrLeu: 3.636 ± 0.635
0.943TyrMet: 0.943 ± 0.351
1.549TyrAsn: 1.549 ± 0.266
0.875TyrPro: 0.875 ± 0.28
1.481TyrGln: 1.481 ± 0.344
1.885TyrArg: 1.885 ± 0.375
1.818TyrSer: 1.818 ± 0.314
2.087TyrThr: 2.087 ± 0.324
3.097TyrVal: 3.097 ± 0.495
0.741TyrTrp: 0.741 ± 0.226
1.616TyrTyr: 1.616 ± 0.478
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (14852 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski