Castor canadensis papillomavirus 1
Average proteome isoelectric point is 5.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V9P8X8|V9P8X8_9PAPI Protein E7 OS=Castor canadensis papillomavirus 1 OX=1352235 GN=E7 PE=3 SV=1
MM1 pKa = 7.61 IGPHH5 pKa = 6.43 PTLKK9 pKa = 10.72 DD10 pKa = 2.98 IVLQEE15 pKa = 4.07 VPEE18 pKa = 4.27 VVDD21 pKa = 4.15 LNCYY25 pKa = 7.88 EE26 pKa = 4.15 QVPPEE31 pKa = 5.1 DD32 pKa = 3.4 ILLEE36 pKa = 4.14 EE37 pKa = 4.46 VGRR40 pKa = 11.84 VSDD43 pKa = 4.53 LYY45 pKa = 11.17 QVVCHH50 pKa = 6.45 CARR53 pKa = 11.84 CDD55 pKa = 3.45 HH56 pKa = 7.37 LIRR59 pKa = 11.84 LTLIAEE65 pKa = 4.36 HH66 pKa = 6.85 AALQVLHH73 pKa = 6.49 QLLCGDD79 pKa = 4.27 ISIVCPSCAARR90 pKa = 11.84 LL91 pKa = 3.67
Molecular weight: 10.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.703
IPC2_protein 4.774
IPC_protein 4.647
Toseland 4.558
ProMoST 4.787
Dawson 4.635
Bjellqvist 4.762
Wikipedia 4.52
Rodwell 4.533
Grimsley 4.469
Solomon 4.622
Lehninger 4.584
Nozaki 4.749
DTASelect 4.902
Thurlkill 4.571
EMBOSS 4.546
Sillero 4.813
Patrickios 0.566
IPC_peptide 4.635
IPC2_peptide 4.8
IPC2.peptide.svr19 4.711
Protein with the highest isoelectric point:
>tr|V9P889|V9P889_9PAPI Minor capsid protein L2 OS=Castor canadensis papillomavirus 1 OX=1352235 GN=L2 PE=3 SV=1
MM1 pKa = 7.28 EE2 pKa = 4.34 QQSLPKK8 pKa = 9.61 TVRR11 pKa = 11.84 DD12 pKa = 3.92 LSAHH16 pKa = 5.4 LQVTFVQLLLPCKK29 pKa = 10.09 FCYY32 pKa = 10.33 SFLTTNDD39 pKa = 3.37 KK40 pKa = 11.21 LLFDD44 pKa = 4.84 LFGLQLVYY52 pKa = 10.59 RR53 pKa = 11.84 GGYY56 pKa = 9.14 PYY58 pKa = 10.6 GACQPCLSSCCKK70 pKa = 10.01 IEE72 pKa = 4.11 FLMFYY77 pKa = 10.54 EE78 pKa = 4.68 DD79 pKa = 3.64 TLDD82 pKa = 3.51 GHH84 pKa = 6.39 GVVATTGKK92 pKa = 10.08 SLQEE96 pKa = 3.87 HH97 pKa = 6.58 PVRR100 pKa = 11.84 CVLCAKK106 pKa = 10.5 LLTPSEE112 pKa = 4.29 KK113 pKa = 10.72 DD114 pKa = 3.43 SVVHH118 pKa = 6.75 FGEE121 pKa = 4.6 KK122 pKa = 6.9 VHH124 pKa = 6.46 KK125 pKa = 10.32 VRR127 pKa = 11.84 GRR129 pKa = 11.84 FKK131 pKa = 10.84 AKK133 pKa = 10.39 CGLCRR138 pKa = 11.84 LACII142 pKa = 4.76
Molecular weight: 15.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.663
IPC2_protein 7.614
IPC_protein 7.439
Toseland 6.942
ProMoST 8.039
Dawson 8.141
Bjellqvist 8.639
Wikipedia 8.039
Rodwell 8.156
Grimsley 6.942
Solomon 8.2
Lehninger 8.229
Nozaki 8.99
DTASelect 8.273
Thurlkill 8.331
EMBOSS 8.361
Sillero 8.726
Patrickios 3.465
IPC_peptide 8.2
IPC2_peptide 7.936
IPC2.peptide.svr19 7.873
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1870
91
612
374.0
41.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.364 ± 0.414
2.193 ± 0.759
6.898 ± 0.519
5.348 ± 0.317
5.027 ± 0.365
6.898 ± 1.011
2.246 ± 0.338
5.08 ± 0.63
4.064 ± 0.836
9.144 ± 0.98
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.711 ± 0.396
3.85 ± 0.598
6.043 ± 0.782
4.706 ± 0.345
6.043 ± 0.394
7.754 ± 0.82
5.722 ± 0.238
6.631 ± 0.785
1.07 ± 0.376
3.209 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here