Helicobacter suis HS5
Average proteome isoelectric point is 7.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1136 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7G5B7|E7G5B7_9HELI Lipid A biosynthesis lauroyl acyltransferase OS=Helicobacter suis HS5 OX=710394 GN=htrB PE=4 SV=1
MM1 pKa = 7.62 LPLKK5 pKa = 10.32 IPKK8 pKa = 9.48 NYY10 pKa = 10.21 KK11 pKa = 8.89 EE12 pKa = 4.74 FYY14 pKa = 9.79 MSLLVNQEE22 pKa = 4.62 CIACDD27 pKa = 3.48 ACRR30 pKa = 11.84 EE31 pKa = 4.08 EE32 pKa = 5.1 CPTDD36 pKa = 5.9 AIDD39 pKa = 4.45 QDD41 pKa = 4.0 DD42 pKa = 5.32 PIYY45 pKa = 11.0 SIDD48 pKa = 3.62 PDD50 pKa = 3.51 RR51 pKa = 11.84 CTEE54 pKa = 3.99 CVGYY58 pKa = 10.24 SDD60 pKa = 5.34 EE61 pKa = 4.68 PGCVSVCPVDD71 pKa = 5.93 AIILDD76 pKa = 3.68 PHH78 pKa = 5.43 NTEE81 pKa = 4.12 SQEE84 pKa = 3.88 EE85 pKa = 4.17 LQYY88 pKa = 11.3 KK89 pKa = 10.17 YY90 pKa = 10.3 LTLQEE95 pKa = 4.64 KK96 pKa = 9.89 II97 pKa = 3.83
Molecular weight: 11.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.946
IPC2_protein 4.024
IPC_protein 3.948
Toseland 3.757
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.088
Wikipedia 3.846
Rodwell 3.783
Grimsley 3.681
Solomon 3.923
Lehninger 3.872
Nozaki 4.05
DTASelect 4.228
Thurlkill 3.808
EMBOSS 3.859
Sillero 4.075
Patrickios 0.172
IPC_peptide 3.923
IPC2_peptide 4.05
IPC2.peptide.svr19 3.975
Protein with the highest isoelectric point:
>tr|E7G282|E7G282_9HELI Hypothetical YkgG-like transporter protein OS=Helicobacter suis HS5 OX=710394 GN=HSUHS5_0006 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.37 RR14 pKa = 11.84 THH16 pKa = 6.01 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.29 TKK25 pKa = 10.52 NGRR28 pKa = 11.84 KK29 pKa = 8.88 VIKK32 pKa = 10.14 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 RR40 pKa = 11.84 QLAVV44 pKa = 2.95
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1136
0
1136
360682
22
1868
317.5
35.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.361 ± 0.061
1.223 ± 0.031
4.554 ± 0.049
6.04 ± 0.08
4.973 ± 0.051
6.259 ± 0.076
2.593 ± 0.041
7.072 ± 0.06
7.458 ± 0.067
11.498 ± 0.097
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.443 ± 0.032
4.51 ± 0.063
3.843 ± 0.039
4.443 ± 0.052
3.928 ± 0.048
6.517 ± 0.054
4.695 ± 0.046
6.068 ± 0.062
0.774 ± 0.024
3.747 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here