Torque teno midi virus 9
Average proteome isoelectric point is 7.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7VLY4|A7VLY4_9VIRU Capsid protein OS=Torque teno midi virus 9 OX=2065050 PE=3 SV=1
MM1 pKa = 6.26 TAYY4 pKa = 11.0 YY5 pKa = 10.7 NFARR9 pKa = 11.84 KK10 pKa = 7.37 TPYY13 pKa = 10.49 NNCTHH18 pKa = 5.76 EE19 pKa = 4.21 QLWISSFNDD28 pKa = 2.9 SHH30 pKa = 7.75 DD31 pKa = 3.99 AFCGCTKK38 pKa = 10.28 PVTHH42 pKa = 6.68 MLSILFPEE50 pKa = 4.56 GHH52 pKa = 7.15 RR53 pKa = 11.84 DD54 pKa = 3.12 LDD56 pKa = 3.59 LTIRR60 pKa = 11.84 QILQRR65 pKa = 11.84 EE66 pKa = 4.19 KK67 pKa = 11.01 EE68 pKa = 3.98 DD69 pKa = 4.02 TICLFGGTDD78 pKa = 3.33 EE79 pKa = 5.23 RR80 pKa = 11.84 DD81 pKa = 3.29 GGEE84 pKa = 4.02 AQEE87 pKa = 5.31 DD88 pKa = 4.03 PGTKK92 pKa = 10.07 EE93 pKa = 4.32 KK94 pKa = 10.68 DD95 pKa = 3.16 IPDD98 pKa = 3.48 ATEE101 pKa = 3.82 EE102 pKa = 4.35 EE103 pKa = 4.46 FTKK106 pKa = 10.26 IDD108 pKa = 4.37 VEE110 pKa = 4.42 DD111 pKa = 4.31 LLAAAADD118 pKa = 3.87 ADD120 pKa = 4.2 TPRR123 pKa = 5.39
Molecular weight: 13.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.412
IPC2_protein 4.393
IPC_protein 4.329
Toseland 4.164
ProMoST 4.457
Dawson 4.304
Bjellqvist 4.444
Wikipedia 4.202
Rodwell 4.164
Grimsley 4.075
Solomon 4.291
Lehninger 4.253
Nozaki 4.406
DTASelect 4.596
Thurlkill 4.177
EMBOSS 4.215
Sillero 4.457
Patrickios 3.656
IPC_peptide 4.291
IPC2_peptide 4.444
IPC2.peptide.svr19 4.353
Protein with the highest isoelectric point:
>tr|A7VLY4|A7VLY4_9VIRU Capsid protein OS=Torque teno midi virus 9 OX=2065050 PE=3 SV=1
MM1 pKa = 7.42 PFWWNRR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 10.02 RR10 pKa = 11.84 WWRR13 pKa = 11.84 GARR16 pKa = 11.84 RR17 pKa = 11.84 PWYY20 pKa = 9.28 KK21 pKa = 9.87 RR22 pKa = 11.84 KK23 pKa = 10.09 RR24 pKa = 11.84 YY25 pKa = 5.49 TRR27 pKa = 11.84 RR28 pKa = 11.84 YY29 pKa = 8.79 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 IYY34 pKa = 10.06 KK35 pKa = 9.4 NRR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 PTRR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 HH50 pKa = 4.09 TKK52 pKa = 8.95 VRR54 pKa = 11.84 RR55 pKa = 11.84 KK56 pKa = 9.79 RR57 pKa = 11.84 QTIPVRR63 pKa = 11.84 QWQPEE68 pKa = 4.08 RR69 pKa = 11.84 IVRR72 pKa = 11.84 CKK74 pKa = 10.24 IKK76 pKa = 10.45 GISVIVLGGEE86 pKa = 4.59 GKK88 pKa = 9.84 QFQCYY93 pKa = 7.34 TNEE96 pKa = 3.94 ATTWTPLRR104 pKa = 11.84 TPGGGGFGVEE114 pKa = 4.91 KK115 pKa = 9.97 YY116 pKa = 8.82 TLSHH120 pKa = 7.24 LYY122 pKa = 10.15 DD123 pKa = 3.17 QYY125 pKa = 12.11 KK126 pKa = 9.93 FGNNIWTKK134 pKa = 9.71 TNILTDD140 pKa = 3.74 LVQYY144 pKa = 9.32 LGCKK148 pKa = 9.34 FVFYY152 pKa = 10.28 RR153 pKa = 11.84 HH154 pKa = 5.74 QYY156 pKa = 8.78 VDD158 pKa = 4.79 FIVKK162 pKa = 9.78 YY163 pKa = 10.62 SRR165 pKa = 11.84 NLPMTIEE172 pKa = 4.24 KK173 pKa = 8.92 LTYY176 pKa = 9.93 PHH178 pKa = 6.53 THH180 pKa = 6.21 PQALLLSRR188 pKa = 11.84 HH189 pKa = 5.48 KK190 pKa = 10.52 RR191 pKa = 11.84 IIPSRR196 pKa = 11.84 VTKK199 pKa = 9.59 PHH201 pKa = 5.51 GKK203 pKa = 9.07 NRR205 pKa = 11.84 VIIKK209 pKa = 10.03 IKK211 pKa = 7.5 PTRR214 pKa = 11.84 VVLKK218 pKa = 10.05 KK219 pKa = 9.81 WFFQEE224 pKa = 4.32 TFDD227 pKa = 3.95 TTGLCSIYY235 pKa = 10.81 AAACDD240 pKa = 4.15 LNYY243 pKa = 10.27 PHH245 pKa = 7.52 IGATSQNQIITLFGLNQSFYY265 pKa = 11.7 SKK267 pKa = 10.85 GNWGAVVQTGYY278 pKa = 10.61 QPYY281 pKa = 9.97 PSVTTFTYY289 pKa = 8.76 KK290 pKa = 9.83 TAATKK295 pKa = 10.09 QVEE298 pKa = 4.75 TFNKK302 pKa = 6.69 PTTYY306 pKa = 9.65 EE307 pKa = 3.76 ASVSYY312 pKa = 7.61 TTGWFNPKK320 pKa = 9.79 LMQLTEE326 pKa = 4.29 YY327 pKa = 8.73 VTPQQLAKK335 pKa = 9.93 PVLAFRR341 pKa = 11.84 YY342 pKa = 8.66 NAQLDD347 pKa = 3.78 NGKK350 pKa = 10.67 GNSVWFKK357 pKa = 11.3 SILTEE362 pKa = 4.48 DD363 pKa = 3.82 FSKK366 pKa = 11.05 PKK368 pKa = 10.16 VDD370 pKa = 3.42 SDD372 pKa = 5.43 LIISDD377 pKa = 3.65 MPIWQALYY385 pKa = 10.84 GFTDD389 pKa = 4.95 FIKK392 pKa = 10.6 AVKK395 pKa = 9.58 PDD397 pKa = 3.95 PNFLASYY404 pKa = 9.94 ILLIEE409 pKa = 4.26 TRR411 pKa = 11.84 FVEE414 pKa = 4.66 PFSSHH419 pKa = 4.97 ATGNYY424 pKa = 7.61 WIPIDD429 pKa = 3.85 SSFVTGKK436 pKa = 10.71 GPYY439 pKa = 7.45 NTPIGDD445 pKa = 3.61 YY446 pKa = 10.38 TKK448 pKa = 10.26 TKK450 pKa = 9.64 WFPTVKK456 pKa = 10.25 NQQEE460 pKa = 4.5 SINAFVKK467 pKa = 10.38 AGPYY471 pKa = 9.48 IPRR474 pKa = 11.84 LQDD477 pKa = 3.22 EE478 pKa = 4.94 KK479 pKa = 11.18 KK480 pKa = 9.56 STWEE484 pKa = 3.37 LHH486 pKa = 6.38 AFYY489 pKa = 10.53 QFYY492 pKa = 10.58 LKK494 pKa = 10.27 WGGTYY499 pKa = 9.08 TEE501 pKa = 4.38 EE502 pKa = 4.16 TQVADD507 pKa = 3.83 PSKK510 pKa = 10.02 QGTYY514 pKa = 10.93 VMPSFIQEE522 pKa = 3.89 KK523 pKa = 10.03 LQIINPAKK531 pKa = 10.05 QKK533 pKa = 10.3 AAAMLHH539 pKa = 5.63 CWDD542 pKa = 3.89 YY543 pKa = 11.43 RR544 pKa = 11.84 RR545 pKa = 11.84 GLITQSAIKK554 pKa = 9.72 RR555 pKa = 11.84 ICEE558 pKa = 4.0 DD559 pKa = 3.42 QPTDD563 pKa = 3.11 TDD565 pKa = 3.93 FQTGSEE571 pKa = 4.19 SSQPLKK577 pKa = 10.64 KK578 pKa = 10.04 KK579 pKa = 10.63 KK580 pKa = 9.1 KK581 pKa = 4.62 TTKK584 pKa = 9.79 EE585 pKa = 3.47 LPYY588 pKa = 9.98 PPKK591 pKa = 10.53 EE592 pKa = 3.97 KK593 pKa = 11.0 EE594 pKa = 4.04 EE595 pKa = 3.98 LQKK598 pKa = 11.11 CLLSLYY604 pKa = 9.95 EE605 pKa = 3.95 EE606 pKa = 5.22 SISQEE611 pKa = 3.53 IPQTQNLQEE620 pKa = 5.42 LIQQQQQQQQQLKK633 pKa = 9.22 YY634 pKa = 9.43 NILKK638 pKa = 10.2 IISEE642 pKa = 4.64 LKK644 pKa = 9.95 QQQQLLGLQTGLVLL658 pKa = 4.97
Molecular weight: 77.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.196
IPC2_protein 9.502
IPC_protein 9.502
Toseland 10.058
ProMoST 9.765
Dawson 10.277
Bjellqvist 9.955
Wikipedia 10.452
Rodwell 10.73
Grimsley 10.35
Solomon 10.292
Lehninger 10.248
Nozaki 10.072
DTASelect 9.94
Thurlkill 10.116
EMBOSS 10.467
Sillero 10.189
Patrickios 10.175
IPC_peptide 10.292
IPC2_peptide 8.624
IPC2.peptide.svr19 8.472
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1322
123
658
330.5
38.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.749 ± 1.154
1.891 ± 0.422
4.917 ± 1.194
5.825 ± 0.921
4.236 ± 0.405
4.539 ± 0.575
2.572 ± 0.67
5.295 ± 0.826
8.623 ± 0.825
7.337 ± 0.193
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.059 ± 0.096
4.085 ± 0.249
5.976 ± 0.869
6.581 ± 0.989
6.203 ± 0.773
6.732 ± 2.464
9.228 ± 0.594
3.48 ± 0.749
1.664 ± 0.688
4.009 ± 1.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here