Salipiger marinus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Salipiger

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5375 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G8QFN7|A0A1G8QFN7_9RHOB Peptide/nickel transport system permease protein OS=Salipiger marinus OX=555512 GN=SAMN04487993_101596 PE=3 SV=1
MM1 pKa = 7.34AHH3 pKa = 5.94VFDD6 pKa = 4.79YY7 pKa = 10.8RR8 pKa = 11.84NSSSGVHH15 pKa = 6.43ANLSYY20 pKa = 11.35LFVQNTSYY28 pKa = 10.49TVANNLFYY36 pKa = 10.7QDD38 pKa = 3.36GVTAGTYY45 pKa = 9.63TYY47 pKa = 10.93FDD49 pKa = 4.36DD50 pKa = 7.23FYY52 pKa = 9.86QQWVSSPTSQATTGVVSFGVMDD74 pKa = 3.53QSNSSDD80 pKa = 3.55VYY82 pKa = 11.04FRR84 pKa = 11.84DD85 pKa = 3.77AYY87 pKa = 10.78GDD89 pKa = 3.96DD90 pKa = 3.56VPDD93 pKa = 4.2RR94 pKa = 11.84FDD96 pKa = 3.5EE97 pKa = 4.36STEE100 pKa = 3.71FTGIYY105 pKa = 9.91GGPDD109 pKa = 3.6YY110 pKa = 10.89IWGYY114 pKa = 10.44FRR116 pKa = 11.84LFGIAAPQLDD126 pKa = 3.99WDD128 pKa = 4.15NTAVAADD135 pKa = 4.19PNVAAQQFDD144 pKa = 4.09ANIAASAADD153 pKa = 3.81YY154 pKa = 10.16AAQLDD159 pKa = 4.12AVLAMVVQNRR169 pKa = 11.84IEE171 pKa = 4.06YY172 pKa = 9.98VDD174 pKa = 3.56WNTGLTADD182 pKa = 4.57GQPTAPQTPGFVMEE196 pKa = 4.28YY197 pKa = 8.85TPSAGSSLTYY207 pKa = 10.93GDD209 pKa = 4.29FLSYY213 pKa = 9.81TQDD216 pKa = 2.92SFGRR220 pKa = 11.84TGLASQGDD228 pKa = 4.21GDD230 pKa = 4.42GGLGGIFRR238 pKa = 11.84ADD240 pKa = 3.36EE241 pKa = 4.26TVEE244 pKa = 5.34LLLTDD249 pKa = 4.01QADD252 pKa = 3.76LVNATEE258 pKa = 4.67LSAGTLILDD267 pKa = 4.29LAGGNDD273 pKa = 3.99LAQLFYY279 pKa = 11.06DD280 pKa = 5.22DD281 pKa = 5.84SYY283 pKa = 11.78LLPGFLDD290 pKa = 4.14LDD292 pKa = 4.27AGSGDD297 pKa = 3.55DD298 pKa = 4.37QIYY301 pKa = 10.64LGEE304 pKa = 4.34FMDD307 pKa = 5.52AEE309 pKa = 4.37ITLGSGDD316 pKa = 3.79DD317 pKa = 4.16VIYY320 pKa = 9.35LTYY323 pKa = 10.38GYY325 pKa = 9.01YY326 pKa = 10.84ASVPPVTPFQHH337 pKa = 6.84TIRR340 pKa = 11.84GGAGNDD346 pKa = 3.64VIVGGPSQSVLTAFGGEE363 pKa = 4.05GRR365 pKa = 11.84DD366 pKa = 3.51WIYY369 pKa = 11.36GGAHH373 pKa = 6.92ADD375 pKa = 3.57VLDD378 pKa = 4.13GGKK381 pKa = 10.44GADD384 pKa = 3.59YY385 pKa = 11.14LFGSTGDD392 pKa = 3.61DD393 pKa = 3.58TYY395 pKa = 11.58HH396 pKa = 5.12VTEE399 pKa = 3.9SDD401 pKa = 3.94RR402 pKa = 11.84IEE404 pKa = 4.33EE405 pKa = 4.25YY406 pKa = 10.8AGEE409 pKa = 4.36GRR411 pKa = 11.84DD412 pKa = 3.43TAIAAGSFTLQPEE425 pKa = 4.41VEE427 pKa = 4.09NLILTGTGNTKK438 pKa = 9.03GTGNASANHH447 pKa = 4.83ITGNAGANVLKK458 pKa = 10.88GGGGNDD464 pKa = 3.59TLVGGAGNDD473 pKa = 3.51TYY475 pKa = 11.14VTDD478 pKa = 3.96GGDD481 pKa = 3.85RR482 pKa = 11.84IIEE485 pKa = 4.14KK486 pKa = 10.54AGDD489 pKa = 4.16GIDD492 pKa = 3.47TVRR495 pKa = 11.84ASVGFALGANIEE507 pKa = 4.04HH508 pKa = 7.43LILIGTGDD516 pKa = 3.35INGTGNTRR524 pKa = 11.84ANKK527 pKa = 8.69LTGNSGANVLKK538 pKa = 10.72GGEE541 pKa = 4.37GNDD544 pKa = 3.73TLTGGAGQDD553 pKa = 2.68AFVFNTMPGAGNVDD567 pKa = 4.89RR568 pKa = 11.84ITDD571 pKa = 3.98FDD573 pKa = 3.9VAQDD577 pKa = 3.88VIQLKK582 pKa = 9.17TSVFAGLPRR591 pKa = 11.84GVLSEE596 pKa = 5.0DD597 pKa = 3.47AFAQNGSGRR606 pKa = 11.84ATEE609 pKa = 3.98AQHH612 pKa = 6.74RR613 pKa = 11.84VIHH616 pKa = 5.48EE617 pKa = 4.09TDD619 pKa = 3.19TGKK622 pKa = 10.68LFYY625 pKa = 10.98DD626 pKa = 3.63SDD628 pKa = 4.1GTGAAAAVHH637 pKa = 6.4FATVTRR643 pKa = 11.84DD644 pKa = 3.48LLALTSEE651 pKa = 4.54DD652 pKa = 3.45FLLFF656 pKa = 4.25

Molecular weight: 68.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G8IZK8|A0A1G8IZK8_9RHOB Pseudouridine synthase OS=Salipiger marinus OX=555512 GN=SAMN04487993_1002215 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36GGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5375

0

5375

1678086

14

4039

312.2

33.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.249 ± 0.05

0.884 ± 0.011

5.915 ± 0.028

5.65 ± 0.031

3.498 ± 0.022

8.883 ± 0.034

2.049 ± 0.016

4.588 ± 0.026

2.354 ± 0.025

10.906 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.66 ± 0.02

2.114 ± 0.02

5.509 ± 0.028

3.291 ± 0.017

7.482 ± 0.044

4.942 ± 0.023

5.372 ± 0.024

7.225 ± 0.029

1.395 ± 0.015

2.034 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski