Malvastrum leaf curl betasatellite
Average proteome isoelectric point is 5.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2PAY5|Q2PAY5_9VIRU C1 protein OS=Malvastrum leaf curl betasatellite OX=339965 GN=c1 PE=4 SV=1
MM1 pKa = 7.12 TIKK4 pKa = 9.84 YY5 pKa = 5.36 TTRR8 pKa = 11.84 KK9 pKa = 9.3 GLSFIINVRR18 pKa = 11.84 LEE20 pKa = 3.73 PHH22 pKa = 6.58 RR23 pKa = 11.84 EE24 pKa = 3.63 VSTEE28 pKa = 3.48 IQFYY32 pKa = 7.54 STKK35 pKa = 10.46 TPFLQKK41 pKa = 10.07 QYY43 pKa = 10.89 FRR45 pKa = 11.84 IPYY48 pKa = 6.36 THH50 pKa = 6.53 QGIITPFNFNALEE63 pKa = 4.04 EE64 pKa = 4.91 GIKK67 pKa = 10.5 NLIHH71 pKa = 7.69 IMYY74 pKa = 8.72 TDD76 pKa = 3.17 SHH78 pKa = 8.26 HH79 pKa = 7.01 EE80 pKa = 3.95 DD81 pKa = 3.35 FRR83 pKa = 11.84 YY84 pKa = 10.41 EE85 pKa = 4.04 DD86 pKa = 4.76 LIEE89 pKa = 5.58 AIDD92 pKa = 3.69 ILMMHH97 pKa = 7.1 EE98 pKa = 4.52 APVLHH103 pKa = 6.74 INLGEE108 pKa = 4.09 EE109 pKa = 4.18 YY110 pKa = 10.03 QVYY113 pKa = 10.74 SSTTMM118 pKa = 3.31
Molecular weight: 14.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.49
IPC2_protein 5.499
IPC_protein 5.499
Toseland 5.995
ProMoST 5.817
Dawson 5.728
Bjellqvist 5.741
Wikipedia 5.728
Rodwell 5.715
Grimsley 6.224
Solomon 5.728
Lehninger 5.715
Nozaki 5.982
DTASelect 6.173
Thurlkill 6.186
EMBOSS 6.135
Sillero 6.084
Patrickios 3.567
IPC_peptide 5.753
IPC2_peptide 6.109
IPC2.peptide.svr19 6.055
Protein with the highest isoelectric point:
>tr|Q2PAY5|Q2PAY5_9VIRU C1 protein OS=Malvastrum leaf curl betasatellite OX=339965 GN=c1 PE=4 SV=1
MM1 pKa = 7.12 TIKK4 pKa = 9.84 YY5 pKa = 5.36 TTRR8 pKa = 11.84 KK9 pKa = 9.3 GLSFIINVRR18 pKa = 11.84 LEE20 pKa = 3.73 PHH22 pKa = 6.58 RR23 pKa = 11.84 EE24 pKa = 3.63 VSTEE28 pKa = 3.48 IQFYY32 pKa = 7.54 STKK35 pKa = 10.46 TPFLQKK41 pKa = 10.07 QYY43 pKa = 10.89 FRR45 pKa = 11.84 IPYY48 pKa = 6.36 THH50 pKa = 6.53 QGIITPFNFNALEE63 pKa = 4.04 EE64 pKa = 4.91 GIKK67 pKa = 10.5 NLIHH71 pKa = 7.69 IMYY74 pKa = 8.72 TDD76 pKa = 3.17 SHH78 pKa = 8.26 HH79 pKa = 7.01 EE80 pKa = 3.95 DD81 pKa = 3.35 FRR83 pKa = 11.84 YY84 pKa = 10.41 EE85 pKa = 4.04 DD86 pKa = 4.76 LIEE89 pKa = 5.58 AIDD92 pKa = 3.69 ILMMHH97 pKa = 7.1 EE98 pKa = 4.52 APVLHH103 pKa = 6.74 INLGEE108 pKa = 4.09 EE109 pKa = 4.18 YY110 pKa = 10.03 QVYY113 pKa = 10.74 SSTTMM118 pKa = 3.31
Molecular weight: 14.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.49
IPC2_protein 5.499
IPC_protein 5.499
Toseland 5.995
ProMoST 5.817
Dawson 5.728
Bjellqvist 5.741
Wikipedia 5.728
Rodwell 5.715
Grimsley 6.224
Solomon 5.728
Lehninger 5.715
Nozaki 5.982
DTASelect 6.173
Thurlkill 6.186
EMBOSS 6.135
Sillero 6.084
Patrickios 3.567
IPC_peptide 5.753
IPC2_peptide 6.109
IPC2.peptide.svr19 6.055
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
118
118
118
118.0
14.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.542 ± 0.0
0.0 ± 0.0
3.39 ± 0.0
9.322 ± 0.0
5.932 ± 0.0
3.39 ± 0.0
5.932 ± 0.0
11.864 ± 0.0
4.237 ± 0.0
7.627 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.237 ± 0.0
4.237 ± 0.0
4.237 ± 0.0
4.237 ± 0.0
4.237 ± 0.0
5.085 ± 0.0
9.322 ± 0.0
3.39 ± 0.0
0.0 ± 0.0
6.78 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here