Rhizobium phage RR1-A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9TNA8|R9TNA8_9CAUD Uncharacterized protein OS=Rhizobium phage RR1-A OX=929833 GN=RHXG_00047 PE=4 SV=1
MM1 pKa = 7.3TSEE4 pKa = 4.2KK5 pKa = 9.14TWYY8 pKa = 6.41PTFMIDD14 pKa = 4.11EE15 pKa = 4.99DD16 pKa = 4.2LPEE19 pKa = 4.95GKK21 pKa = 9.97SSPEE25 pKa = 3.1AWLPFLTQEE34 pKa = 4.31EE35 pKa = 4.47AGQPAMFNAGDD46 pKa = 3.34ILAFEE51 pKa = 4.47FVEE54 pKa = 3.96RR55 pKa = 11.84RR56 pKa = 11.84GNIEE60 pKa = 3.37ITINPDD66 pKa = 2.91GSHH69 pKa = 6.43GNAPRR74 pKa = 11.84CADD77 pKa = 3.45TADD80 pKa = 3.67MFGEE84 pKa = 4.48DD85 pKa = 3.95AARR88 pKa = 11.84TTPVEE93 pKa = 4.09KK94 pKa = 10.06NTEE97 pKa = 4.07RR98 pKa = 11.84EE99 pKa = 4.41CNSFWDD105 pKa = 4.2YY106 pKa = 11.39EE107 pKa = 4.48SEE109 pKa = 4.42TFAEE113 pKa = 4.37TLDD116 pKa = 3.91EE117 pKa = 4.14FAKK120 pKa = 10.42IYY122 pKa = 10.63AEE124 pKa = 4.18CANEE128 pKa = 3.87VEE130 pKa = 4.94PISVVISTAYY140 pKa = 8.92WSPTFRR146 pKa = 11.84FRR148 pKa = 11.84IADD151 pKa = 3.63DD152 pKa = 4.02CKK154 pKa = 11.48SLIQQPATDD163 pKa = 3.88AEE165 pKa = 4.55SAEE168 pKa = 4.42EE169 pKa = 3.91

Molecular weight: 19.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9TP86|R9TP86_9CAUD N-acetyltransferase domain-containing protein OS=Rhizobium phage RR1-A OX=929833 GN=RHXG_00055 PE=4 SV=1
MM1 pKa = 7.2AWKK4 pKa = 10.27RR5 pKa = 11.84PDD7 pKa = 3.26GAQRR11 pKa = 11.84PLPRR15 pKa = 11.84AIRR18 pKa = 11.84SNLQRR23 pKa = 11.84MEE25 pKa = 5.43RR26 pKa = 11.84IMQAANSKK34 pKa = 8.49EE35 pKa = 3.94EE36 pKa = 4.42KK37 pKa = 10.2LIQDD41 pKa = 3.39MADD44 pKa = 2.56AMRR47 pKa = 11.84RR48 pKa = 11.84YY49 pKa = 10.59GEE51 pKa = 4.44GCTSEE56 pKa = 3.9EE57 pKa = 4.27LNRR60 pKa = 11.84HH61 pKa = 5.07FTQSEE66 pKa = 3.98IAHH69 pKa = 5.63YY70 pKa = 9.42GARR73 pKa = 11.84ARR75 pKa = 11.84ARR77 pKa = 11.84ANDD80 pKa = 3.15QAVRR84 pKa = 11.84HH85 pKa = 5.05IRR87 pKa = 11.84KK88 pKa = 9.14RR89 pKa = 11.84AAA91 pKa = 3.12

Molecular weight: 10.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

16568

54

869

243.6

26.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.991 ± 0.42

1.183 ± 0.122

6.307 ± 0.162

6.41 ± 0.285

3.591 ± 0.187

8.009 ± 0.264

2.137 ± 0.168

5.354 ± 0.219

4.34 ± 0.218

7.991 ± 0.253

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.65 ± 0.138

3.024 ± 0.143

5.136 ± 0.199

3.513 ± 0.14

7.352 ± 0.284

6.193 ± 0.206

5.402 ± 0.219

6.549 ± 0.254

1.539 ± 0.129

2.33 ± 0.107
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski