Miniopterus schreibersii polyomavirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Alphapolyomavirus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S7J007|A0A1S7J007_9POLY Small T antigen OS=Miniopterus schreibersii polyomavirus 1 OX=1904408 PE=4 SV=1
MM1 pKa = 7.45GGVLSTVVEE10 pKa = 4.84MITMAVEE17 pKa = 3.9LSAATGIAMDD27 pKa = 4.35ALLTGEE33 pKa = 4.62ALAALEE39 pKa = 4.48AEE41 pKa = 4.87VTSLMTIQGLSAFEE55 pKa = 4.56ALAQLGWTAEE65 pKa = 4.19QFSNMAFISTTFSQAIGYY83 pKa = 9.53GVMFQTVTGISGLVQAGIRR102 pKa = 11.84LGLNVASSSRR112 pKa = 11.84GTQVAQLEE120 pKa = 4.28RR121 pKa = 11.84DD122 pKa = 3.25FGKK125 pKa = 10.2IVEE128 pKa = 4.42TLHH131 pKa = 6.82VNLSHH136 pKa = 6.94QMNPLNWCASLHH148 pKa = 5.86EE149 pKa = 4.8EE150 pKa = 4.42FPPSIQHH157 pKa = 6.19LPISLRR163 pKa = 11.84HH164 pKa = 5.2QFADD168 pKa = 3.64LLKK171 pKa = 10.49LGRR174 pKa = 11.84WVNQHH179 pKa = 6.37HH180 pKa = 6.4FTTNPAFEE188 pKa = 4.89SGDD191 pKa = 3.39VIRR194 pKa = 11.84RR195 pKa = 11.84FEE197 pKa = 4.22APGGAFQDD205 pKa = 4.04VAPDD209 pKa = 3.37WLVNLILRR217 pKa = 11.84LHH219 pKa = 7.04DD220 pKa = 3.93GAEE223 pKa = 4.07EE224 pKa = 3.91ATPFCSSLSSS234 pKa = 3.35

Molecular weight: 25.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S7J009|A0A1S7J009_9POLY Putative alternative large T antigen OS=Miniopterus schreibersii polyomavirus 1 OX=1904408 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 5.46PPASRR7 pKa = 11.84KK8 pKa = 8.17STGHH12 pKa = 5.73GRR14 pKa = 11.84QEE16 pKa = 4.0YY17 pKa = 10.93LPMKK21 pKa = 10.03DD22 pKa = 4.26LNPNQIYY29 pKa = 8.28TVMNPVTPTVEE40 pKa = 4.44SPPSHH45 pKa = 7.08PLNPQVITLLPPAEE59 pKa = 4.95DD60 pKa = 3.63SQPPPLPPKK69 pKa = 8.76RR70 pKa = 11.84TPPRR74 pKa = 11.84RR75 pKa = 11.84SVSLPSRR82 pKa = 11.84KK83 pKa = 8.92PLPMHH88 pKa = 6.69PLQMEE93 pKa = 4.41VEE95 pKa = 4.25TLEE98 pKa = 4.09RR99 pKa = 11.84NLRR102 pKa = 11.84QKK104 pKa = 9.44EE105 pKa = 3.82QDD107 pKa = 3.58MVRR110 pKa = 11.84MWMDD114 pKa = 3.47LVQARR119 pKa = 11.84KK120 pKa = 7.98QASRR124 pKa = 11.84ARR126 pKa = 11.84LQRR129 pKa = 11.84KK130 pKa = 5.9KK131 pKa = 10.61QKK133 pKa = 9.2WVTVLLICLLVFLSILAMLYY153 pKa = 10.83LLIKK157 pKa = 10.73LLTTSS162 pKa = 3.42

Molecular weight: 18.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1870

162

710

311.7

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.059 ± 0.794

2.62 ± 0.774

4.118 ± 0.396

6.31 ± 0.588

4.652 ± 0.749

5.989 ± 1.042

2.513 ± 0.349

4.064 ± 0.557

6.043 ± 1.38

11.818 ± 0.636

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.674 ± 0.402

3.904 ± 0.384

6.684 ± 1.44

5.027 ± 0.573

3.957 ± 0.535

7.326 ± 0.62

5.775 ± 0.25

5.829 ± 0.437

1.444 ± 0.379

2.193 ± 0.505
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski