Bovine faeces associated circular DNA molecule 1
Average proteome isoelectric point is 5.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A168MGH2|A0A168MGH2_9VIRU Replication associated protein OS=Bovine faeces associated circular DNA molecule 1 OX=1843763 PE=4 SV=1
MM1 pKa = 8.05 PSQQAKK7 pKa = 8.01 VWTMTVKK14 pKa = 10.42 NWDD17 pKa = 3.68 LFDD20 pKa = 3.79 NQEE23 pKa = 3.82 WEE25 pKa = 4.3 EE26 pKa = 4.18 RR27 pKa = 11.84 IQNQTEE33 pKa = 3.88 PDD35 pKa = 3.22 GSKK38 pKa = 10.07 IIRR41 pKa = 11.84 YY42 pKa = 9.33 LSIGKK47 pKa = 8.08 HH48 pKa = 5.01 TGQQTGYY55 pKa = 7.19 QHH57 pKa = 6.61 CHH59 pKa = 5.67 FNLEE63 pKa = 4.19 LEE65 pKa = 4.6 KK66 pKa = 10.52 KK67 pKa = 8.93 KK68 pKa = 9.56 TMAWIKK74 pKa = 10.48 RR75 pKa = 11.84 EE76 pKa = 3.99 LFNRR80 pKa = 11.84 EE81 pKa = 4.55 DD82 pKa = 3.18 IHH84 pKa = 7.78 CEE86 pKa = 3.38 PRR88 pKa = 11.84 RR89 pKa = 11.84 GSRR92 pKa = 11.84 EE93 pKa = 3.68 QCDD96 pKa = 3.42 SYY98 pKa = 11.54 LNKK101 pKa = 10.79 DD102 pKa = 3.51 GEE104 pKa = 4.54 FKK106 pKa = 11.14 VIINTRR112 pKa = 11.84 QIKK115 pKa = 9.24 PGRR118 pKa = 11.84 RR119 pKa = 11.84 TDD121 pKa = 3.78 LDD123 pKa = 5.18 DD124 pKa = 3.31 IHH126 pKa = 9.68 DD127 pKa = 4.48 MIKK130 pKa = 10.47 EE131 pKa = 4.03 GASLYY136 pKa = 10.74 DD137 pKa = 3.58 CYY139 pKa = 10.79 EE140 pKa = 3.85 EE141 pKa = 5.23 HH142 pKa = 6.79 FGTVVRR148 pKa = 11.84 CEE150 pKa = 3.74 RR151 pKa = 11.84 GLRR154 pKa = 11.84 DD155 pKa = 3.79 YY156 pKa = 10.93 IALRR160 pKa = 11.84 DD161 pKa = 3.9 TILATKK167 pKa = 9.79 KK168 pKa = 10.11 KK169 pKa = 9.13 YY170 pKa = 8.43 PAPEE174 pKa = 3.98 VIVYY178 pKa = 8.34 VGPSGSGKK186 pKa = 9.63 SWHH189 pKa = 6.79 CSEE192 pKa = 5.46 DD193 pKa = 3.35 PDD195 pKa = 4.39 YY196 pKa = 11.81 EE197 pKa = 4.09 EE198 pKa = 4.18 GGYY201 pKa = 10.19 RR202 pKa = 11.84 FSIQMDD208 pKa = 3.38 SKK210 pKa = 11.09 IYY212 pKa = 10.17 FDD214 pKa = 4.78 GYY216 pKa = 11.33 NNQKK220 pKa = 7.65 TLWFDD225 pKa = 3.45 EE226 pKa = 4.91 FSGKK230 pKa = 8.12 TMPFTKK236 pKa = 10.15 FCQIADD242 pKa = 4.56 RR243 pKa = 11.84 YY244 pKa = 8.04 PGRR247 pKa = 11.84 YY248 pKa = 4.83 EE249 pKa = 4.02 TKK251 pKa = 9.69 GGSVLIYY258 pKa = 9.95 GLKK261 pKa = 10.17 KK262 pKa = 10.08 ILISTVEE269 pKa = 4.28 YY270 pKa = 9.13 PALWWSGSDD279 pKa = 4.05 RR280 pKa = 11.84 YY281 pKa = 11.21 NKK283 pKa = 10.46 DD284 pKa = 2.94 PEE286 pKa = 4.12 QLFRR290 pKa = 11.84 RR291 pKa = 11.84 ITKK294 pKa = 10.02 CYY296 pKa = 10.18 YY297 pKa = 10.17 LGQPRR302 pKa = 11.84 IKK304 pKa = 10.16 EE305 pKa = 4.36 DD306 pKa = 3.26 GDD308 pKa = 3.37 IEE310 pKa = 4.22 YY311 pKa = 10.37 AIPLEE316 pKa = 4.1 FNPRR320 pKa = 11.84 HH321 pKa = 6.29 LRR323 pKa = 11.84 TQYY326 pKa = 11.05 DD327 pKa = 3.74 EE328 pKa = 5.75 EE329 pKa = 4.16 ILKK332 pKa = 11.16 GNVKK336 pKa = 10.31 YY337 pKa = 10.45 PSDD340 pKa = 3.69 LMEE343 pKa = 4.77 EE344 pKa = 4.3 EE345 pKa = 5.32 PIATAEE351 pKa = 4.11 EE352 pKa = 4.15 AVKK355 pKa = 10.18 EE356 pKa = 4.0 LKK358 pKa = 10.45 KK359 pKa = 10.83 LLEE362 pKa = 3.92 QSRR365 pKa = 11.84 EE366 pKa = 3.84 EE367 pKa = 5.55 DD368 pKa = 3.25 EE369 pKa = 4.12 EE370 pKa = 4.45 QPIKK374 pKa = 10.46 RR375 pKa = 11.84 RR376 pKa = 11.84 RR377 pKa = 11.84 SRR379 pKa = 11.84 SRR381 pKa = 11.84 SRR383 pKa = 11.84 EE384 pKa = 3.75 DD385 pKa = 4.9 DD386 pKa = 3.41 EE387 pKa = 7.24 DD388 pKa = 3.84 EE389 pKa = 6.41 AEE391 pKa = 5.07 DD392 pKa = 5.37 DD393 pKa = 4.03 YY394 pKa = 12.18 CEE396 pKa = 6.14 LIDD399 pKa = 5.82 DD400 pKa = 4.11 NN401 pKa = 5.01
Molecular weight: 47.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.094
IPC2_protein 5.169
IPC_protein 5.118
Toseland 5.054
ProMoST 5.258
Dawson 5.105
Bjellqvist 5.232
Wikipedia 4.965
Rodwell 5.029
Grimsley 4.978
Solomon 5.105
Lehninger 5.067
Nozaki 5.219
DTASelect 5.359
Thurlkill 5.054
EMBOSS 5.003
Sillero 5.296
Patrickios 4.024
IPC_peptide 5.118
IPC2_peptide 5.296
IPC2.peptide.svr19 5.27
Protein with the highest isoelectric point:
>tr|A0A168MGH2|A0A168MGH2_9VIRU Replication associated protein OS=Bovine faeces associated circular DNA molecule 1 OX=1843763 PE=4 SV=1
MM1 pKa = 8.05 PSQQAKK7 pKa = 8.01 VWTMTVKK14 pKa = 10.42 NWDD17 pKa = 3.68 LFDD20 pKa = 3.79 NQEE23 pKa = 3.82 WEE25 pKa = 4.3 EE26 pKa = 4.18 RR27 pKa = 11.84 IQNQTEE33 pKa = 3.88 PDD35 pKa = 3.22 GSKK38 pKa = 10.07 IIRR41 pKa = 11.84 YY42 pKa = 9.33 LSIGKK47 pKa = 8.08 HH48 pKa = 5.01 TGQQTGYY55 pKa = 7.19 QHH57 pKa = 6.61 CHH59 pKa = 5.67 FNLEE63 pKa = 4.19 LEE65 pKa = 4.6 KK66 pKa = 10.52 KK67 pKa = 8.93 KK68 pKa = 9.56 TMAWIKK74 pKa = 10.48 RR75 pKa = 11.84 EE76 pKa = 3.99 LFNRR80 pKa = 11.84 EE81 pKa = 4.55 DD82 pKa = 3.18 IHH84 pKa = 7.78 CEE86 pKa = 3.38 PRR88 pKa = 11.84 RR89 pKa = 11.84 GSRR92 pKa = 11.84 EE93 pKa = 3.68 QCDD96 pKa = 3.42 SYY98 pKa = 11.54 LNKK101 pKa = 10.79 DD102 pKa = 3.51 GEE104 pKa = 4.54 FKK106 pKa = 11.14 VIINTRR112 pKa = 11.84 QIKK115 pKa = 9.24 PGRR118 pKa = 11.84 RR119 pKa = 11.84 TDD121 pKa = 3.78 LDD123 pKa = 5.18 DD124 pKa = 3.31 IHH126 pKa = 9.68 DD127 pKa = 4.48 MIKK130 pKa = 10.47 EE131 pKa = 4.03 GASLYY136 pKa = 10.74 DD137 pKa = 3.58 CYY139 pKa = 10.79 EE140 pKa = 3.85 EE141 pKa = 5.23 HH142 pKa = 6.79 FGTVVRR148 pKa = 11.84 CEE150 pKa = 3.74 RR151 pKa = 11.84 GLRR154 pKa = 11.84 DD155 pKa = 3.79 YY156 pKa = 10.93 IALRR160 pKa = 11.84 DD161 pKa = 3.9 TILATKK167 pKa = 9.79 KK168 pKa = 10.11 KK169 pKa = 9.13 YY170 pKa = 8.43 PAPEE174 pKa = 3.98 VIVYY178 pKa = 8.34 VGPSGSGKK186 pKa = 9.63 SWHH189 pKa = 6.79 CSEE192 pKa = 5.46 DD193 pKa = 3.35 PDD195 pKa = 4.39 YY196 pKa = 11.81 EE197 pKa = 4.09 EE198 pKa = 4.18 GGYY201 pKa = 10.19 RR202 pKa = 11.84 FSIQMDD208 pKa = 3.38 SKK210 pKa = 11.09 IYY212 pKa = 10.17 FDD214 pKa = 4.78 GYY216 pKa = 11.33 NNQKK220 pKa = 7.65 TLWFDD225 pKa = 3.45 EE226 pKa = 4.91 FSGKK230 pKa = 8.12 TMPFTKK236 pKa = 10.15 FCQIADD242 pKa = 4.56 RR243 pKa = 11.84 YY244 pKa = 8.04 PGRR247 pKa = 11.84 YY248 pKa = 4.83 EE249 pKa = 4.02 TKK251 pKa = 9.69 GGSVLIYY258 pKa = 9.95 GLKK261 pKa = 10.17 KK262 pKa = 10.08 ILISTVEE269 pKa = 4.28 YY270 pKa = 9.13 PALWWSGSDD279 pKa = 4.05 RR280 pKa = 11.84 YY281 pKa = 11.21 NKK283 pKa = 10.46 DD284 pKa = 2.94 PEE286 pKa = 4.12 QLFRR290 pKa = 11.84 RR291 pKa = 11.84 ITKK294 pKa = 10.02 CYY296 pKa = 10.18 YY297 pKa = 10.17 LGQPRR302 pKa = 11.84 IKK304 pKa = 10.16 EE305 pKa = 4.36 DD306 pKa = 3.26 GDD308 pKa = 3.37 IEE310 pKa = 4.22 YY311 pKa = 10.37 AIPLEE316 pKa = 4.1 FNPRR320 pKa = 11.84 HH321 pKa = 6.29 LRR323 pKa = 11.84 TQYY326 pKa = 11.05 DD327 pKa = 3.74 EE328 pKa = 5.75 EE329 pKa = 4.16 ILKK332 pKa = 11.16 GNVKK336 pKa = 10.31 YY337 pKa = 10.45 PSDD340 pKa = 3.69 LMEE343 pKa = 4.77 EE344 pKa = 4.3 EE345 pKa = 5.32 PIATAEE351 pKa = 4.11 EE352 pKa = 4.15 AVKK355 pKa = 10.18 EE356 pKa = 4.0 LKK358 pKa = 10.45 KK359 pKa = 10.83 LLEE362 pKa = 3.92 QSRR365 pKa = 11.84 EE366 pKa = 3.84 EE367 pKa = 5.55 DD368 pKa = 3.25 EE369 pKa = 4.12 EE370 pKa = 4.45 QPIKK374 pKa = 10.46 RR375 pKa = 11.84 RR376 pKa = 11.84 RR377 pKa = 11.84 SRR379 pKa = 11.84 SRR381 pKa = 11.84 SRR383 pKa = 11.84 EE384 pKa = 3.75 DD385 pKa = 4.9 DD386 pKa = 3.41 EE387 pKa = 7.24 DD388 pKa = 3.84 EE389 pKa = 6.41 AEE391 pKa = 5.07 DD392 pKa = 5.37 DD393 pKa = 4.03 YY394 pKa = 12.18 CEE396 pKa = 6.14 LIDD399 pKa = 5.82 DD400 pKa = 4.11 NN401 pKa = 5.01
Molecular weight: 47.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.094
IPC2_protein 5.169
IPC_protein 5.118
Toseland 5.054
ProMoST 5.258
Dawson 5.105
Bjellqvist 5.232
Wikipedia 4.965
Rodwell 5.029
Grimsley 4.978
Solomon 5.105
Lehninger 5.067
Nozaki 5.219
DTASelect 5.359
Thurlkill 5.054
EMBOSS 5.003
Sillero 5.296
Patrickios 4.024
IPC_peptide 5.118
IPC2_peptide 5.296
IPC2.peptide.svr19 5.27
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
401
401
401
401.0
47.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.242 ± 0.0
2.244 ± 0.0
8.229 ± 0.0
10.973 ± 0.0
3.242 ± 0.0
6.234 ± 0.0
1.995 ± 0.0
6.983 ± 0.0
7.98 ± 0.0
6.484 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.746 ± 0.0
3.242 ± 0.0
4.489 ± 0.0
4.489 ± 0.0
7.481 ± 0.0
5.486 ± 0.0
4.738 ± 0.0
2.993 ± 0.0
1.995 ± 0.0
5.736 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here