Amino acid dipepetide frequency for Gordonia phage Keelan

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.765AlaAla: 8.765 ± 1.13
0.663AlaCys: 0.663 ± 0.201
4.691AlaAsp: 4.691 ± 0.445
6.917AlaGlu: 6.917 ± 0.682
3.269AlaPhe: 3.269 ± 0.413
6.681AlaGly: 6.681 ± 1.065
1.706AlaHis: 1.706 ± 0.289
4.785AlaIle: 4.785 ± 0.441
4.738AlaLys: 4.738 ± 0.533
7.912AlaLeu: 7.912 ± 0.66
2.89AlaMet: 2.89 ± 0.353
3.885AlaAsn: 3.885 ± 0.415
2.795AlaPro: 2.795 ± 0.413
3.364AlaGln: 3.364 ± 0.374
4.833AlaArg: 4.833 ± 0.421
5.733AlaSer: 5.733 ± 0.716
6.065AlaThr: 6.065 ± 0.73
4.928AlaVal: 4.928 ± 0.518
1.421AlaTrp: 1.421 ± 0.252
2.938AlaTyr: 2.938 ± 0.434
0.0AlaXaa: 0.0 ± 0.0
Cys
1.137CysAla: 1.137 ± 0.303
0.284CysCys: 0.284 ± 0.112
1.09CysAsp: 1.09 ± 0.222
0.711CysGlu: 0.711 ± 0.203
0.284CysPhe: 0.284 ± 0.127
1.753CysGly: 1.753 ± 0.353
0.332CysHis: 0.332 ± 0.138
0.426CysIle: 0.426 ± 0.132
0.663CysLys: 0.663 ± 0.188
0.758CysLeu: 0.758 ± 0.231
0.19CysMet: 0.19 ± 0.101
0.663CysAsn: 0.663 ± 0.159
1.09CysPro: 1.09 ± 0.244
0.474CysGln: 0.474 ± 0.17
0.995CysArg: 0.995 ± 0.25
0.663CysSer: 0.663 ± 0.186
0.616CysThr: 0.616 ± 0.19
0.758CysVal: 0.758 ± 0.221
0.237CysTrp: 0.237 ± 0.127
0.474CysTyr: 0.474 ± 0.143
0.0CysXaa: 0.0 ± 0.0
Asp
6.444AspAla: 6.444 ± 0.652
0.805AspCys: 0.805 ± 0.182
4.833AspAsp: 4.833 ± 0.479
4.548AspGlu: 4.548 ± 0.522
2.416AspPhe: 2.416 ± 0.349
5.307AspGly: 5.307 ± 0.505
1.374AspHis: 1.374 ± 0.241
3.032AspIle: 3.032 ± 0.366
2.606AspLys: 2.606 ± 0.342
5.117AspLeu: 5.117 ± 0.575
2.037AspMet: 2.037 ± 0.277
2.653AspAsn: 2.653 ± 0.302
3.364AspPro: 3.364 ± 0.366
2.938AspGln: 2.938 ± 0.348
3.743AspArg: 3.743 ± 0.48
3.648AspSer: 3.648 ± 0.406
2.559AspThr: 2.559 ± 0.347
3.885AspVal: 3.885 ± 0.404
1.421AspTrp: 1.421 ± 0.226
2.085AspTyr: 2.085 ± 0.409
0.0AspXaa: 0.0 ± 0.0
Glu
5.449GluAla: 5.449 ± 0.494
1.232GluCys: 1.232 ± 0.279
4.88GluAsp: 4.88 ± 0.502
4.691GluGlu: 4.691 ± 0.65
3.127GluPhe: 3.127 ± 0.426
3.553GluGly: 3.553 ± 0.355
1.8GluHis: 1.8 ± 0.298
3.743GluIle: 3.743 ± 0.498
3.174GluLys: 3.174 ± 0.392
6.491GluLeu: 6.491 ± 0.472
3.032GluMet: 3.032 ± 0.38
2.653GluAsn: 2.653 ± 0.419
2.274GluPro: 2.274 ± 0.378
2.89GluGln: 2.89 ± 0.39
4.643GluArg: 4.643 ± 0.495
5.07GluSer: 5.07 ± 0.442
3.79GluThr: 3.79 ± 0.447
5.022GluVal: 5.022 ± 0.505
1.706GluTrp: 1.706 ± 0.34
2.559GluTyr: 2.559 ± 0.396
0.0GluXaa: 0.0 ± 0.0
Phe
2.416PheAla: 2.416 ± 0.327
0.663PheCys: 0.663 ± 0.2
2.89PheAsp: 2.89 ± 0.305
2.843PheGlu: 2.843 ± 0.368
0.758PhePhe: 0.758 ± 0.195
2.985PheGly: 2.985 ± 0.324
0.474PheHis: 0.474 ± 0.144
2.227PheIle: 2.227 ± 0.309
1.469PheLys: 1.469 ± 0.269
2.511PheLeu: 2.511 ± 0.394
0.853PheMet: 0.853 ± 0.199
1.137PheAsn: 1.137 ± 0.182
1.421PhePro: 1.421 ± 0.298
1.042PheGln: 1.042 ± 0.211
1.469PheArg: 1.469 ± 0.268
2.037PheSer: 2.037 ± 0.312
1.8PheThr: 1.8 ± 0.273
2.322PheVal: 2.322 ± 0.316
0.474PheTrp: 0.474 ± 0.146
0.948PheTyr: 0.948 ± 0.203
0.0PheXaa: 0.0 ± 0.0
Gly
6.538GlyAla: 6.538 ± 0.755
0.474GlyCys: 0.474 ± 0.142
4.548GlyAsp: 4.548 ± 0.533
5.401GlyGlu: 5.401 ± 0.503
2.606GlyPhe: 2.606 ± 0.339
7.202GlyGly: 7.202 ± 1.634
1.516GlyHis: 1.516 ± 0.29
4.643GlyIle: 4.643 ± 0.475
4.738GlyLys: 4.738 ± 0.481
5.828GlyLeu: 5.828 ± 0.524
2.606GlyMet: 2.606 ± 0.393
3.317GlyAsn: 3.317 ± 0.491
2.606GlyPro: 2.606 ± 0.378
2.748GlyGln: 2.748 ± 0.457
3.79GlyArg: 3.79 ± 0.435
4.454GlySer: 4.454 ± 0.674
6.112GlyThr: 6.112 ± 0.556
4.738GlyVal: 4.738 ± 0.506
1.848GlyTrp: 1.848 ± 0.321
3.459GlyTyr: 3.459 ± 0.448
0.0GlyXaa: 0.0 ± 0.0
His
1.516HisAla: 1.516 ± 0.235
0.237HisCys: 0.237 ± 0.106
0.948HisAsp: 0.948 ± 0.26
1.658HisGlu: 1.658 ± 0.272
0.426HisPhe: 0.426 ± 0.132
1.99HisGly: 1.99 ± 0.308
0.237HisHis: 0.237 ± 0.117
0.948HisIle: 0.948 ± 0.194
1.042HisLys: 1.042 ± 0.256
1.706HisLeu: 1.706 ± 0.262
0.853HisMet: 0.853 ± 0.177
0.521HisAsn: 0.521 ± 0.162
1.184HisPro: 1.184 ± 0.23
0.569HisGln: 0.569 ± 0.181
1.327HisArg: 1.327 ± 0.347
1.184HisSer: 1.184 ± 0.237
1.09HisThr: 1.09 ± 0.21
1.137HisVal: 1.137 ± 0.233
0.616HisTrp: 0.616 ± 0.141
0.474HisTyr: 0.474 ± 0.166
0.0HisXaa: 0.0 ± 0.0
Ile
4.027IleAla: 4.027 ± 0.399
0.663IleCys: 0.663 ± 0.16
3.743IleAsp: 3.743 ± 0.348
4.264IleGlu: 4.264 ± 0.421
1.232IlePhe: 1.232 ± 0.213
4.454IleGly: 4.454 ± 0.581
1.09IleHis: 1.09 ± 0.23
1.658IleIle: 1.658 ± 0.347
1.753IleLys: 1.753 ± 0.275
4.312IleLeu: 4.312 ± 0.507
0.9IleMet: 0.9 ± 0.176
1.658IleAsn: 1.658 ± 0.24
2.985IlePro: 2.985 ± 0.311
2.037IleGln: 2.037 ± 0.281
3.174IleArg: 3.174 ± 0.357
3.79IleSer: 3.79 ± 0.374
3.127IleThr: 3.127 ± 0.438
3.838IleVal: 3.838 ± 0.419
1.137IleTrp: 1.137 ± 0.266
1.327IleTyr: 1.327 ± 0.26
0.0IleXaa: 0.0 ± 0.0
Lys
4.359LysAla: 4.359 ± 0.45
0.711LysCys: 0.711 ± 0.208
3.269LysAsp: 3.269 ± 0.371
3.885LysGlu: 3.885 ± 0.535
1.374LysPhe: 1.374 ± 0.254
3.696LysGly: 3.696 ± 0.447
1.042LysHis: 1.042 ± 0.239
1.753LysIle: 1.753 ± 0.28
2.416LysLys: 2.416 ± 0.466
4.027LysLeu: 4.027 ± 0.455
1.421LysMet: 1.421 ± 0.24
2.085LysAsn: 2.085 ± 0.293
2.701LysPro: 2.701 ± 0.405
1.279LysGln: 1.279 ± 0.24
3.885LysArg: 3.885 ± 0.521
2.843LysSer: 2.843 ± 0.419
2.701LysThr: 2.701 ± 0.428
3.838LysVal: 3.838 ± 0.504
0.995LysTrp: 0.995 ± 0.222
1.658LysTyr: 1.658 ± 0.241
0.0LysXaa: 0.0 ± 0.0
Leu
8.102LeuAla: 8.102 ± 0.621
1.09LeuCys: 1.09 ± 0.226
5.259LeuAsp: 5.259 ± 0.505
6.207LeuGlu: 6.207 ± 0.726
2.085LeuPhe: 2.085 ± 0.318
6.302LeuGly: 6.302 ± 0.684
1.611LeuHis: 1.611 ± 0.293
3.648LeuIle: 3.648 ± 0.468
3.696LeuLys: 3.696 ± 0.362
5.496LeuLeu: 5.496 ± 0.565
2.085LeuMet: 2.085 ± 0.389
3.222LeuAsn: 3.222 ± 0.449
4.454LeuPro: 4.454 ± 0.415
2.464LeuGln: 2.464 ± 0.281
4.217LeuArg: 4.217 ± 0.47
4.975LeuSer: 4.975 ± 0.418
4.312LeuThr: 4.312 ± 0.443
5.496LeuVal: 5.496 ± 0.563
1.516LeuTrp: 1.516 ± 0.333
2.085LeuTyr: 2.085 ± 0.363
0.0LeuXaa: 0.0 ± 0.0
Met
3.08MetAla: 3.08 ± 0.362
0.379MetCys: 0.379 ± 0.142
1.943MetAsp: 1.943 ± 0.33
1.469MetGlu: 1.469 ± 0.301
0.948MetPhe: 0.948 ± 0.187
2.179MetGly: 2.179 ± 0.437
0.426MetHis: 0.426 ± 0.152
1.279MetIle: 1.279 ± 0.253
1.8MetLys: 1.8 ± 0.261
2.085MetLeu: 2.085 ± 0.344
0.426MetMet: 0.426 ± 0.159
1.469MetAsn: 1.469 ± 0.257
1.564MetPro: 1.564 ± 0.291
0.663MetGln: 0.663 ± 0.167
1.042MetArg: 1.042 ± 0.222
2.274MetSer: 2.274 ± 0.32
1.895MetThr: 1.895 ± 0.308
1.564MetVal: 1.564 ± 0.307
0.379MetTrp: 0.379 ± 0.151
0.711MetTyr: 0.711 ± 0.187
0.0MetXaa: 0.0 ± 0.0
Asn
3.032AsnAla: 3.032 ± 0.456
0.758AsnCys: 0.758 ± 0.234
2.274AsnAsp: 2.274 ± 0.364
2.748AsnGlu: 2.748 ± 0.344
0.616AsnPhe: 0.616 ± 0.183
4.359AsnGly: 4.359 ± 0.57
0.805AsnHis: 0.805 ± 0.208
1.564AsnIle: 1.564 ± 0.288
1.374AsnLys: 1.374 ± 0.226
2.795AsnLeu: 2.795 ± 0.397
0.805AsnMet: 0.805 ± 0.186
1.848AsnAsn: 1.848 ± 0.275
2.511AsnPro: 2.511 ± 0.324
1.943AsnGln: 1.943 ± 0.311
2.559AsnArg: 2.559 ± 0.345
2.843AsnSer: 2.843 ± 0.353
2.369AsnThr: 2.369 ± 0.336
2.938AsnVal: 2.938 ± 0.382
0.616AsnTrp: 0.616 ± 0.231
1.184AsnTyr: 1.184 ± 0.188
0.0AsnXaa: 0.0 ± 0.0
Pro
3.696ProAla: 3.696 ± 0.562
0.9ProCys: 0.9 ± 0.231
3.032ProAsp: 3.032 ± 0.464
2.89ProGlu: 2.89 ± 0.337
1.469ProPhe: 1.469 ± 0.296
3.79ProGly: 3.79 ± 0.406
0.805ProHis: 0.805 ± 0.192
2.322ProIle: 2.322 ± 0.406
2.322ProLys: 2.322 ± 0.374
2.511ProLeu: 2.511 ± 0.271
0.9ProMet: 0.9 ± 0.187
1.895ProAsn: 1.895 ± 0.292
1.753ProPro: 1.753 ± 0.327
1.469ProGln: 1.469 ± 0.259
1.943ProArg: 1.943 ± 0.29
3.032ProSer: 3.032 ± 0.359
2.985ProThr: 2.985 ± 0.365
4.122ProVal: 4.122 ± 0.481
1.042ProTrp: 1.042 ± 0.212
1.564ProTyr: 1.564 ± 0.275
0.0ProXaa: 0.0 ± 0.0
Gln
3.127GlnAla: 3.127 ± 0.477
0.474GlnCys: 0.474 ± 0.137
1.706GlnAsp: 1.706 ± 0.26
2.701GlnGlu: 2.701 ± 0.342
1.421GlnPhe: 1.421 ± 0.32
2.701GlnGly: 2.701 ± 0.36
0.332GlnHis: 0.332 ± 0.112
2.132GlnIle: 2.132 ± 0.339
2.227GlnLys: 2.227 ± 0.318
2.274GlnLeu: 2.274 ± 0.295
0.948GlnMet: 0.948 ± 0.233
1.232GlnAsn: 1.232 ± 0.288
1.895GlnPro: 1.895 ± 0.301
1.564GlnGln: 1.564 ± 0.354
2.322GlnArg: 2.322 ± 0.291
2.179GlnSer: 2.179 ± 0.368
1.327GlnThr: 1.327 ± 0.219
2.416GlnVal: 2.416 ± 0.331
1.184GlnTrp: 1.184 ± 0.182
1.232GlnTyr: 1.232 ± 0.188
0.0GlnXaa: 0.0 ± 0.0
Arg
4.928ArgAla: 4.928 ± 0.444
0.9ArgCys: 0.9 ± 0.243
3.601ArgAsp: 3.601 ± 0.422
4.928ArgGlu: 4.928 ± 0.626
2.085ArgPhe: 2.085 ± 0.291
4.359ArgGly: 4.359 ± 0.478
0.948ArgHis: 0.948 ± 0.238
3.127ArgIle: 3.127 ± 0.428
3.648ArgLys: 3.648 ± 0.478
3.885ArgLeu: 3.885 ± 0.374
1.706ArgMet: 1.706 ± 0.339
2.369ArgAsn: 2.369 ± 0.285
2.037ArgPro: 2.037 ± 0.279
2.132ArgGln: 2.132 ± 0.289
3.506ArgArg: 3.506 ± 0.513
3.411ArgSer: 3.411 ± 0.363
2.606ArgThr: 2.606 ± 0.406
3.317ArgVal: 3.317 ± 0.396
1.469ArgTrp: 1.469 ± 0.3
1.753ArgTyr: 1.753 ± 0.268
0.0ArgXaa: 0.0 ± 0.0
Ser
6.207SerAla: 6.207 ± 0.579
1.09SerCys: 1.09 ± 0.29
4.975SerAsp: 4.975 ± 0.444
4.312SerGlu: 4.312 ± 0.52
2.227SerPhe: 2.227 ± 0.244
5.638SerGly: 5.638 ± 1.001
1.469SerHis: 1.469 ± 0.298
3.364SerIle: 3.364 ± 0.414
2.89SerLys: 2.89 ± 0.318
5.212SerLeu: 5.212 ± 0.415
1.469SerMet: 1.469 ± 0.296
2.748SerAsn: 2.748 ± 0.437
2.464SerPro: 2.464 ± 0.411
1.516SerGln: 1.516 ± 0.264
3.885SerArg: 3.885 ± 0.387
3.79SerSer: 3.79 ± 0.541
3.553SerThr: 3.553 ± 0.571
3.79SerVal: 3.79 ± 0.48
1.042SerTrp: 1.042 ± 0.235
2.179SerTyr: 2.179 ± 0.322
0.0SerXaa: 0.0 ± 0.0
Thr
5.07ThrAla: 5.07 ± 0.713
0.474ThrCys: 0.474 ± 0.151
3.411ThrAsp: 3.411 ± 0.404
3.222ThrGlu: 3.222 ± 0.436
2.511ThrPhe: 2.511 ± 0.321
3.506ThrGly: 3.506 ± 0.451
0.948ThrHis: 0.948 ± 0.23
3.838ThrIle: 3.838 ± 0.482
2.464ThrLys: 2.464 ± 0.343
4.928ThrLeu: 4.928 ± 0.547
1.421ThrMet: 1.421 ± 0.244
2.464ThrAsn: 2.464 ± 0.308
2.606ThrPro: 2.606 ± 0.395
2.227ThrGln: 2.227 ± 0.276
2.748ThrArg: 2.748 ± 0.379
3.79ThrSer: 3.79 ± 0.533
3.411ThrThr: 3.411 ± 0.423
4.975ThrVal: 4.975 ± 0.589
1.564ThrTrp: 1.564 ± 0.349
2.606ThrTyr: 2.606 ± 0.39
0.0ThrXaa: 0.0 ± 0.0
Val
6.775ValAla: 6.775 ± 0.695
0.805ValCys: 0.805 ± 0.188
4.217ValAsp: 4.217 ± 0.43
4.975ValGlu: 4.975 ± 0.668
2.464ValPhe: 2.464 ± 0.348
5.164ValGly: 5.164 ± 0.41
1.184ValHis: 1.184 ± 0.232
4.027ValIle: 4.027 ± 0.42
3.601ValLys: 3.601 ± 0.39
5.212ValLeu: 5.212 ± 0.562
1.611ValMet: 1.611 ± 0.261
2.274ValAsn: 2.274 ± 0.316
2.464ValPro: 2.464 ± 0.321
2.464ValGln: 2.464 ± 0.273
3.553ValArg: 3.553 ± 0.339
4.691ValSer: 4.691 ± 0.693
4.406ValThr: 4.406 ± 0.581
6.586ValVal: 6.586 ± 0.618
0.9ValTrp: 0.9 ± 0.207
1.943ValTyr: 1.943 ± 0.281
0.0ValXaa: 0.0 ± 0.0
Trp
1.658TrpAla: 1.658 ± 0.31
0.332TrpCys: 0.332 ± 0.138
1.137TrpAsp: 1.137 ± 0.213
0.948TrpGlu: 0.948 ± 0.232
0.805TrpPhe: 0.805 ± 0.187
1.469TrpGly: 1.469 ± 0.281
0.9TrpHis: 0.9 ± 0.206
1.09TrpIle: 1.09 ± 0.219
1.327TrpLys: 1.327 ± 0.23
2.416TrpLeu: 2.416 ± 0.335
0.663TrpMet: 0.663 ± 0.198
0.569TrpAsn: 0.569 ± 0.166
0.711TrpPro: 0.711 ± 0.228
0.663TrpGln: 0.663 ± 0.201
1.184TrpArg: 1.184 ± 0.23
1.327TrpSer: 1.327 ± 0.213
1.469TrpThr: 1.469 ± 0.269
1.184TrpVal: 1.184 ± 0.226
0.474TrpTrp: 0.474 ± 0.177
0.711TrpTyr: 0.711 ± 0.204
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.701TyrAla: 2.701 ± 0.371
0.663TyrCys: 0.663 ± 0.185
2.369TyrAsp: 2.369 ± 0.324
2.464TyrGlu: 2.464 ± 0.396
0.616TyrPhe: 0.616 ± 0.186
2.085TyrGly: 2.085 ± 0.328
0.663TyrHis: 0.663 ± 0.204
1.564TyrIle: 1.564 ± 0.231
1.99TyrLys: 1.99 ± 0.304
2.843TyrLeu: 2.843 ± 0.346
0.711TyrMet: 0.711 ± 0.172
1.279TyrAsn: 1.279 ± 0.277
1.564TyrPro: 1.564 ± 0.235
0.948TyrGln: 0.948 ± 0.199
1.895TyrArg: 1.895 ± 0.26
2.085TyrSer: 2.085 ± 0.351
1.99TyrThr: 1.99 ± 0.345
2.464TyrVal: 2.464 ± 0.344
0.995TyrTrp: 0.995 ± 0.226
1.516TyrTyr: 1.516 ± 0.263
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 145 proteins (21107 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski