Gordonia phage Skog

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 226 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XJR9|A0A6G6XJR9_9CAUD DnaB-like dsDNA helicase OS=Gordonia phage Skog OX=2704033 GN=208 PE=4 SV=1
MM1 pKa = 7.85IDD3 pKa = 5.07FITDD7 pKa = 3.46FQIEE11 pKa = 4.48FGSQMFVPLDD21 pKa = 3.7TEE23 pKa = 4.02ILSITTEE30 pKa = 3.98GNSRR34 pKa = 11.84IFQCHH39 pKa = 5.23CVYY42 pKa = 10.72DD43 pKa = 3.84YY44 pKa = 11.74GPFADD49 pKa = 5.73AYY51 pKa = 9.82APPFEE56 pKa = 4.2FQAMLFQVTLFGEE69 pKa = 4.37FLDD72 pKa = 4.39PQEE75 pKa = 4.46VTCISSPSGDD85 pKa = 3.2GSRR88 pKa = 11.84VTTSKK93 pKa = 10.7EE94 pKa = 3.64

Molecular weight: 10.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XKB8|A0A6G6XKB8_9CAUD Uncharacterized protein OS=Gordonia phage Skog OX=2704033 GN=144 PE=4 SV=1
MM1 pKa = 7.44SSVGLEE7 pKa = 4.29YY8 pKa = 9.97PRR10 pKa = 11.84EE11 pKa = 3.83MARR14 pKa = 11.84IRR16 pKa = 11.84GANRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84LVGILRR28 pKa = 11.84DD29 pKa = 3.48EE30 pKa = 5.13DD31 pKa = 3.61GAATVIVQMARR42 pKa = 11.84DD43 pKa = 4.0TPVEE47 pKa = 4.04TIRR50 pKa = 11.84EE51 pKa = 4.14YY52 pKa = 10.37RR53 pKa = 11.84SSYY56 pKa = 9.6ARR58 pKa = 11.84RR59 pKa = 11.84GAWQDD64 pKa = 3.36RR65 pKa = 11.84HH66 pKa = 5.41WKK68 pKa = 10.14SDD70 pKa = 3.23LL71 pKa = 3.61

Molecular weight: 8.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

226

0

226

47531

32

1902

210.3

23.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.674 ± 0.227

0.86 ± 0.072

7.12 ± 0.208

6.648 ± 0.209

3.118 ± 0.103

8.272 ± 0.3

2.304 ± 0.138

4.345 ± 0.121

3.604 ± 0.134

7.439 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.35 ± 0.105

3.33 ± 0.129

5.626 ± 0.152

4.069 ± 0.154

6.575 ± 0.186

6.167 ± 0.156

6.4 ± 0.211

7.315 ± 0.18

1.805 ± 0.081

2.979 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski