Amino acid dipepetide frequency for Vibrio phage VSKK

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.983AlaAla: 4.983 ± 3.394
0.0AlaCys: 0.0 ± 0.0
2.492AlaAsp: 2.492 ± 1.023
3.322AlaGlu: 3.322 ± 1.176
5.814AlaPhe: 5.814 ± 1.441
3.322AlaGly: 3.322 ± 2.286
1.661AlaHis: 1.661 ± 0.647
7.475AlaIle: 7.475 ± 3.19
5.814AlaLys: 5.814 ± 1.294
4.983AlaLeu: 4.983 ± 1.383
2.492AlaMet: 2.492 ± 0.873
1.661AlaAsn: 1.661 ± 1.109
1.661AlaPro: 1.661 ± 0.647
7.475AlaGln: 7.475 ± 1.216
0.831AlaArg: 0.831 ± 0.7
2.492AlaSer: 2.492 ± 1.657
0.831AlaThr: 0.831 ± 0.7
6.645AlaVal: 6.645 ± 2.125
0.831AlaTrp: 0.831 ± 0.7
2.492AlaTyr: 2.492 ± 1.053
0.0AlaXaa: 0.0 ± 0.0
Cys
0.831CysAla: 0.831 ± 0.618
0.0CysCys: 0.0 ± 0.0
0.831CysAsp: 0.831 ± 0.7
0.0CysGlu: 0.0 ± 0.0
1.661CysPhe: 1.661 ± 0.647
1.661CysGly: 1.661 ± 1.4
0.0CysHis: 0.0 ± 0.0
1.661CysIle: 1.661 ± 0.918
0.831CysLys: 0.831 ± 0.7
0.0CysLeu: 0.0 ± 0.0
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
1.661CysPro: 1.661 ± 1.223
0.0CysGln: 0.0 ± 0.0
0.0CysArg: 0.0 ± 0.0
2.492CysSer: 2.492 ± 1.053
2.492CysThr: 2.492 ± 1.355
1.661CysVal: 1.661 ± 1.235
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
4.983AspAla: 4.983 ± 2.182
0.0AspCys: 0.0 ± 0.0
5.814AspAsp: 5.814 ± 2.707
2.492AspGlu: 2.492 ± 1.853
4.153AspPhe: 4.153 ± 1.047
3.322AspGly: 3.322 ± 2.459
1.661AspHis: 1.661 ± 0.647
5.814AspIle: 5.814 ± 2.23
0.831AspLys: 0.831 ± 0.7
7.475AspLeu: 7.475 ± 2.753
2.492AspMet: 2.492 ± 1.302
0.831AspAsn: 0.831 ± 0.845
2.492AspPro: 2.492 ± 0.85
1.661AspGln: 1.661 ± 0.647
0.831AspArg: 0.831 ± 0.7
2.492AspSer: 2.492 ± 2.1
5.814AspThr: 5.814 ± 2.017
2.492AspVal: 2.492 ± 2.534
0.831AspTrp: 0.831 ± 0.618
1.661AspTyr: 1.661 ± 0.647
0.0AspXaa: 0.0 ± 0.0
Glu
4.983GluAla: 4.983 ± 1.94
2.492GluCys: 2.492 ± 1.198
2.492GluAsp: 2.492 ± 1.653
0.831GluGlu: 0.831 ± 0.7
1.661GluPhe: 1.661 ± 0.647
0.831GluGly: 0.831 ± 0.7
2.492GluHis: 2.492 ± 1.053
1.661GluIle: 1.661 ± 1.239
4.153GluLys: 4.153 ± 0.923
4.983GluLeu: 4.983 ± 2.504
0.831GluMet: 0.831 ± 0.618
1.661GluAsn: 1.661 ± 0.885
2.492GluPro: 2.492 ± 0.85
4.983GluGln: 4.983 ± 1.94
0.0GluArg: 0.0 ± 0.0
4.983GluSer: 4.983 ± 2.755
3.322GluThr: 3.322 ± 2.126
2.492GluVal: 2.492 ± 1.53
0.831GluTrp: 0.831 ± 1.269
1.661GluTyr: 1.661 ± 1.021
0.0GluXaa: 0.0 ± 0.0
Phe
4.983PheAla: 4.983 ± 2.813
0.0PheCys: 0.0 ± 0.0
4.153PheAsp: 4.153 ± 1.794
3.322PheGlu: 3.322 ± 0.949
0.0PhePhe: 0.0 ± 0.0
5.814PheGly: 5.814 ± 1.775
0.831PheHis: 0.831 ± 0.7
1.661PheIle: 1.661 ± 0.647
0.831PheLys: 0.831 ± 1.203
1.661PheLeu: 1.661 ± 1.193
2.492PheMet: 2.492 ± 1.355
2.492PheAsn: 2.492 ± 1.324
0.831PhePro: 0.831 ± 1.047
1.661PheGln: 1.661 ± 0.918
2.492PheArg: 2.492 ± 1.198
6.645PheSer: 6.645 ± 1.975
1.661PheThr: 1.661 ± 0.647
1.661PheVal: 1.661 ± 1.109
0.831PheTrp: 0.831 ± 0.618
4.983PheTyr: 4.983 ± 1.411
0.0PheXaa: 0.0 ± 0.0
Gly
4.153GlyAla: 4.153 ± 1.624
0.831GlyCys: 0.831 ± 0.7
2.492GlyAsp: 2.492 ± 1.053
2.492GlyGlu: 2.492 ± 0.679
2.492GlyPhe: 2.492 ± 0.679
0.831GlyGly: 0.831 ± 0.7
1.661GlyHis: 1.661 ± 1.235
9.967GlyIle: 9.967 ± 3.074
3.322GlyLys: 3.322 ± 1.536
5.814GlyLeu: 5.814 ± 2.705
0.831GlyMet: 0.831 ± 0.7
1.661GlyAsn: 1.661 ± 1.4
0.831GlyPro: 0.831 ± 0.7
3.322GlyGln: 3.322 ± 2.598
2.492GlyArg: 2.492 ± 1.053
5.814GlySer: 5.814 ± 1.775
2.492GlyThr: 2.492 ± 1.267
2.492GlyVal: 2.492 ± 1.589
0.0GlyTrp: 0.0 ± 0.0
4.153GlyTyr: 4.153 ± 1.45
0.0GlyXaa: 0.0 ± 0.0
His
2.492HisAla: 2.492 ± 1.317
0.831HisCys: 0.831 ± 0.618
1.661HisAsp: 1.661 ± 0.885
0.831HisGlu: 0.831 ± 0.7
1.661HisPhe: 1.661 ± 1.689
1.661HisGly: 1.661 ± 0.647
1.661HisHis: 1.661 ± 1.4
0.831HisIle: 0.831 ± 0.618
1.661HisLys: 1.661 ± 1.4
1.661HisLeu: 1.661 ± 1.4
2.492HisMet: 2.492 ± 0.806
0.0HisAsn: 0.0 ± 0.0
0.831HisPro: 0.831 ± 0.7
0.0HisGln: 0.0 ± 0.0
2.492HisArg: 2.492 ± 2.1
0.0HisSer: 0.0 ± 0.0
0.831HisThr: 0.831 ± 0.618
1.661HisVal: 1.661 ± 1.235
0.831HisTrp: 0.831 ± 0.7
2.492HisTyr: 2.492 ± 1.853
0.0HisXaa: 0.0 ± 0.0
Ile
4.983IleAla: 4.983 ± 1.137
2.492IleCys: 2.492 ± 1.063
8.306IleAsp: 8.306 ± 3.639
6.645IleGlu: 6.645 ± 1.685
1.661IlePhe: 1.661 ± 1.389
2.492IleGly: 2.492 ± 1.063
0.831IleHis: 0.831 ± 0.618
4.153IleIle: 4.153 ± 2.523
3.322IleLys: 3.322 ± 1.43
4.153IleLeu: 4.153 ± 2.186
1.661IleMet: 1.661 ± 0.685
4.153IleAsn: 4.153 ± 1.813
6.645IlePro: 6.645 ± 2.103
1.661IleGln: 1.661 ± 0.918
2.492IleArg: 2.492 ± 1.702
2.492IleSer: 2.492 ± 1.653
9.136IleThr: 9.136 ± 1.365
4.153IleVal: 4.153 ± 0.923
1.661IleTrp: 1.661 ± 0.647
4.153IleTyr: 4.153 ± 1.022
0.0IleXaa: 0.0 ± 0.0
Lys
4.153LysAla: 4.153 ± 1.741
1.661LysCys: 1.661 ± 1.4
4.153LysAsp: 4.153 ± 2.637
1.661LysGlu: 1.661 ± 0.647
2.492LysPhe: 2.492 ± 1.267
0.831LysGly: 0.831 ± 1.203
2.492LysHis: 2.492 ± 1.53
4.153LysIle: 4.153 ± 1.022
7.475LysLys: 7.475 ± 1.808
4.983LysLeu: 4.983 ± 1.566
2.492LysMet: 2.492 ± 1.053
3.322LysAsn: 3.322 ± 1.235
0.831LysPro: 0.831 ± 0.618
4.153LysGln: 4.153 ± 2.637
4.983LysArg: 4.983 ± 3.231
3.322LysSer: 3.322 ± 0.99
4.983LysThr: 4.983 ± 1.273
6.645LysVal: 6.645 ± 2.362
0.0LysTrp: 0.0 ± 0.0
2.492LysTyr: 2.492 ± 0.85
0.0LysXaa: 0.0 ± 0.0
Leu
4.153LeuAla: 4.153 ± 3.5
0.0LeuCys: 0.0 ± 0.0
4.153LeuAsp: 4.153 ± 1.624
4.983LeuGlu: 4.983 ± 1.541
2.492LeuPhe: 2.492 ± 2.1
8.306LeuGly: 8.306 ± 0.648
2.492LeuHis: 2.492 ± 1.355
8.306LeuIle: 8.306 ± 1.784
4.153LeuLys: 4.153 ± 1.877
4.983LeuLeu: 4.983 ± 1.987
1.661LeuMet: 1.661 ± 0.832
6.645LeuAsn: 6.645 ± 3.459
1.661LeuPro: 1.661 ± 1.193
1.661LeuGln: 1.661 ± 0.885
3.322LeuArg: 3.322 ± 1.794
4.153LeuSer: 4.153 ± 1.475
4.983LeuThr: 4.983 ± 2.417
4.983LeuVal: 4.983 ± 4.151
1.661LeuTrp: 1.661 ± 1.223
2.492LeuTyr: 2.492 ± 1.544
0.0LeuXaa: 0.0 ± 0.0
Met
2.492MetAla: 2.492 ± 1.55
0.0MetCys: 0.0 ± 0.0
0.0MetAsp: 0.0 ± 0.0
1.661MetGlu: 1.661 ± 1.4
2.492MetPhe: 2.492 ± 1.414
1.661MetGly: 1.661 ± 0.647
0.831MetHis: 0.831 ± 0.618
1.661MetIle: 1.661 ± 1.235
1.661MetLys: 1.661 ± 1.556
1.661MetLeu: 1.661 ± 1.4
0.0MetMet: 0.0 ± 0.0
2.492MetAsn: 2.492 ± 1.653
2.492MetPro: 2.492 ± 1.589
1.661MetGln: 1.661 ± 2.407
0.831MetArg: 0.831 ± 0.7
2.492MetSer: 2.492 ± 1.063
2.492MetThr: 2.492 ± 1.198
2.492MetVal: 2.492 ± 1.198
0.0MetTrp: 0.0 ± 0.0
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
1.661AsnAla: 1.661 ± 1.689
0.0AsnCys: 0.0 ± 0.0
2.492AsnAsp: 2.492 ± 2.1
2.492AsnGlu: 2.492 ± 1.583
0.831AsnPhe: 0.831 ± 0.7
1.661AsnGly: 1.661 ± 1.4
0.831AsnHis: 0.831 ± 0.618
4.153AsnIle: 4.153 ± 2.336
7.475AsnLys: 7.475 ± 3.125
4.153AsnLeu: 4.153 ± 1.028
0.831AsnMet: 0.831 ± 1.203
3.322AsnAsn: 3.322 ± 1.389
1.661AsnPro: 1.661 ± 1.109
1.661AsnGln: 1.661 ± 0.918
2.492AsnArg: 2.492 ± 1.324
2.492AsnSer: 2.492 ± 1.328
4.153AsnThr: 4.153 ± 1.549
2.492AsnVal: 2.492 ± 2.293
0.0AsnTrp: 0.0 ± 0.0
0.0AsnTyr: 0.0 ± 0.0
0.0AsnXaa: 0.0 ± 0.0
Pro
1.661ProAla: 1.661 ± 0.647
0.831ProCys: 0.831 ± 0.7
4.153ProAsp: 4.153 ± 1.993
0.0ProGlu: 0.0 ± 0.0
3.322ProPhe: 3.322 ± 0.99
0.0ProGly: 0.0 ± 0.0
2.492ProHis: 2.492 ± 2.1
0.831ProIle: 0.831 ± 0.618
4.983ProLys: 4.983 ± 2.174
6.645ProLeu: 6.645 ± 3.143
2.492ProMet: 2.492 ± 1.063
0.831ProAsn: 0.831 ± 1.047
2.492ProPro: 2.492 ± 1.063
2.492ProGln: 2.492 ± 1.414
2.492ProArg: 2.492 ± 1.023
4.153ProSer: 4.153 ± 1.041
1.661ProThr: 1.661 ± 1.021
4.983ProVal: 4.983 ± 1.52
0.0ProTrp: 0.0 ± 0.0
0.0ProTyr: 0.0 ± 0.0
0.0ProXaa: 0.0 ± 0.0
Gln
1.661GlnAla: 1.661 ± 1.193
0.831GlnCys: 0.831 ± 0.7
2.492GlnAsp: 2.492 ± 0.679
1.661GlnGlu: 1.661 ± 1.389
2.492GlnPhe: 2.492 ± 1.853
3.322GlnGly: 3.322 ± 2.8
0.831GlnHis: 0.831 ± 0.845
3.322GlnIle: 3.322 ± 1.029
1.661GlnLys: 1.661 ± 0.647
4.153GlnLeu: 4.153 ± 3.198
0.831GlnMet: 0.831 ± 0.7
1.661GlnAsn: 1.661 ± 0.918
2.492GlnPro: 2.492 ± 1.702
1.661GlnGln: 1.661 ± 1.235
3.322GlnArg: 3.322 ± 1.992
4.983GlnSer: 4.983 ± 2.998
0.831GlnThr: 0.831 ± 0.618
4.983GlnVal: 4.983 ± 1.632
0.0GlnTrp: 0.0 ± 0.0
0.831GlnTyr: 0.831 ± 1.047
0.0GlnXaa: 0.0 ± 0.0
Arg
4.153ArgAla: 4.153 ± 2.33
0.0ArgCys: 0.0 ± 0.0
1.661ArgAsp: 1.661 ± 1.193
3.322ArgGlu: 3.322 ± 1.743
3.322ArgPhe: 3.322 ± 0.99
2.492ArgGly: 2.492 ± 2.1
0.831ArgHis: 0.831 ± 0.7
6.645ArgIle: 6.645 ± 1.777
2.492ArgLys: 2.492 ± 1.853
4.983ArgLeu: 4.983 ± 1.52
0.0ArgMet: 0.0 ± 0.0
2.492ArgAsn: 2.492 ± 2.1
5.814ArgPro: 5.814 ± 1.095
0.831ArgGln: 0.831 ± 0.7
4.153ArgArg: 4.153 ± 3.739
2.492ArgSer: 2.492 ± 1.063
3.322ArgThr: 3.322 ± 1.285
0.0ArgVal: 0.0 ± 0.0
0.831ArgTrp: 0.831 ± 0.618
0.831ArgTyr: 0.831 ± 1.269
0.0ArgXaa: 0.0 ± 0.0
Ser
4.153SerAla: 4.153 ± 1.041
0.831SerCys: 0.831 ± 0.618
2.492SerAsp: 2.492 ± 2.534
2.492SerGlu: 2.492 ± 1.317
4.153SerPhe: 4.153 ± 1.45
7.475SerGly: 7.475 ± 3.63
1.661SerHis: 1.661 ± 0.918
2.492SerIle: 2.492 ± 0.679
7.475SerLys: 7.475 ± 1.311
2.492SerLeu: 2.492 ± 1.544
4.153SerMet: 4.153 ± 1.626
3.322SerAsn: 3.322 ± 0.99
1.661SerPro: 1.661 ± 0.647
1.661SerGln: 1.661 ± 1.689
4.983SerArg: 4.983 ± 1.324
3.322SerSer: 3.322 ± 1.235
2.492SerThr: 2.492 ± 2.015
4.153SerVal: 4.153 ± 2.073
0.0SerTrp: 0.0 ± 0.0
2.492SerTyr: 2.492 ± 1.053
0.0SerXaa: 0.0 ± 0.0
Thr
3.322ThrAla: 3.322 ± 0.969
3.322ThrCys: 3.322 ± 1.837
1.661ThrAsp: 1.661 ± 1.109
2.492ThrGlu: 2.492 ± 1.053
3.322ThrPhe: 3.322 ± 1.265
4.983ThrGly: 4.983 ± 2.292
0.0ThrHis: 0.0 ± 0.0
3.322ThrIle: 3.322 ± 0.99
4.153ThrLys: 4.153 ± 1.022
2.492ThrLeu: 2.492 ± 1.589
2.492ThrMet: 2.492 ± 1.986
2.492ThrAsn: 2.492 ± 1.653
1.661ThrPro: 1.661 ± 0.647
3.322ThrGln: 3.322 ± 0.949
4.983ThrArg: 4.983 ± 0.8
0.831ThrSer: 0.831 ± 0.845
3.322ThrThr: 3.322 ± 1.886
5.814ThrVal: 5.814 ± 1.233
0.831ThrTrp: 0.831 ± 0.618
3.322ThrTyr: 3.322 ± 1.796
0.0ThrXaa: 0.0 ± 0.0
Val
3.322ValAla: 3.322 ± 1.595
0.831ValCys: 0.831 ± 0.7
4.983ValAsp: 4.983 ± 2.141
5.814ValGlu: 5.814 ± 2.605
3.322ValPhe: 3.322 ± 1.082
3.322ValGly: 3.322 ± 1.285
0.831ValHis: 0.831 ± 0.845
8.306ValIle: 8.306 ± 2.644
2.492ValLys: 2.492 ± 1.355
4.153ValLeu: 4.153 ± 2.526
0.0ValMet: 0.0 ± 0.0
4.153ValAsn: 4.153 ± 2.946
5.814ValPro: 5.814 ± 1.756
0.831ValGln: 0.831 ± 0.7
3.322ValArg: 3.322 ± 1.853
5.814ValSer: 5.814 ± 1.66
1.661ValThr: 1.661 ± 1.389
1.661ValVal: 1.661 ± 1.021
0.831ValTrp: 0.831 ± 1.203
3.322ValTyr: 3.322 ± 1.601
0.0ValXaa: 0.0 ± 0.0
Trp
0.0TrpAla: 0.0 ± 0.0
0.0TrpCys: 0.0 ± 0.0
0.0TrpAsp: 0.0 ± 0.0
0.0TrpGlu: 0.0 ± 0.0
0.0TrpPhe: 0.0 ± 0.0
1.661TrpGly: 1.661 ± 1.235
0.0TrpHis: 0.0 ± 0.0
0.0TrpIle: 0.0 ± 0.0
0.831TrpLys: 0.831 ± 0.618
1.661TrpLeu: 1.661 ± 1.223
0.0TrpMet: 0.0 ± 0.0
0.0TrpAsn: 0.0 ± 0.0
1.661TrpPro: 1.661 ± 1.239
0.831TrpGln: 0.831 ± 1.269
1.661TrpArg: 1.661 ± 0.647
0.831TrpSer: 0.831 ± 0.7
0.0TrpThr: 0.0 ± 0.0
0.831TrpVal: 0.831 ± 0.7
0.831TrpTrp: 0.831 ± 0.7
0.831TrpTyr: 0.831 ± 0.7
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.153TyrAla: 4.153 ± 1.739
0.831TyrCys: 0.831 ± 0.618
1.661TyrAsp: 1.661 ± 1.021
3.322TyrGlu: 3.322 ± 1.389
1.661TyrPhe: 1.661 ± 1.235
2.492TyrGly: 2.492 ± 1.324
2.492TyrHis: 2.492 ± 1.053
0.831TyrIle: 0.831 ± 0.618
1.661TyrLys: 1.661 ± 1.235
4.153TyrLeu: 4.153 ± 1.988
0.0TyrMet: 0.0 ± 0.0
1.661TyrAsn: 1.661 ± 1.4
0.831TyrPro: 0.831 ± 0.845
2.492TyrGln: 2.492 ± 1.853
2.492TyrArg: 2.492 ± 1.063
2.492TyrSer: 2.492 ± 1.414
1.661TyrThr: 1.661 ± 0.647
2.492TyrVal: 2.492 ± 1.853
0.831TyrTrp: 0.831 ± 0.7
1.661TyrTyr: 1.661 ± 1.235
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6 proteins (1205 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski