Gottschalkia acidurici (strain ATCC 7906 / DSM 604 / BCRC 14475 / CIP 104303 / KCTC 5404 / NCIMB 10678 / 9a) (Clostridium acidurici)
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2839 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K0B1Q9|K0B1Q9_GOTA9 Uncharacterized protein OS=Gottschalkia acidurici (strain ATCC 7906 / DSM 604 / BCRC 14475 / CIP 104303 / KCTC 5404 / NCIMB 10678 / 9a) OX=1128398 GN=Curi_c20480 PE=4 SV=1
MM1 pKa = 7.7 KK2 pKa = 9.48 FTGSNIAYY10 pKa = 9.95 DD11 pKa = 4.2 NYY13 pKa = 10.39 TLDD16 pKa = 3.59 VGNHH20 pKa = 4.92 VLNGKK25 pKa = 9.44 YY26 pKa = 10.25 NSLDD30 pKa = 3.71 LEE32 pKa = 4.45 VSSNNINATINLEE45 pKa = 3.79 GTYY48 pKa = 10.41 GVEE51 pKa = 4.34 FEE53 pKa = 4.52 VDD55 pKa = 3.31 SLSINDD61 pKa = 3.22 VDD63 pKa = 4.83 VSIKK67 pKa = 9.45 GSNMIVDD74 pKa = 4.91 DD75 pKa = 3.68 IVYY78 pKa = 10.36 GVTNN82 pKa = 4.23
Molecular weight: 8.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.541
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.719
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.884
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.872
Patrickios 0.693
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|K0B306|K0B306_GOTA9 Amino acid ABC transporter permease protein YxeN OS=Gottschalkia acidurici (strain ATCC 7906 / DSM 604 / BCRC 14475 / CIP 104303 / KCTC 5404 / NCIMB 10678 / 9a) OX=1128398 GN=yxeN PE=3 SV=1
MM1 pKa = 7.7 AGGRR5 pKa = 11.84 KK6 pKa = 8.63 FRR8 pKa = 11.84 SRR10 pKa = 11.84 KK11 pKa = 9.08 RR12 pKa = 11.84 VCSFCADD19 pKa = 3.43 KK20 pKa = 11.44 NNNEE24 pKa = 3.75 ISYY27 pKa = 11.19 KK28 pKa = 10.55 DD29 pKa = 3.5 VNKK32 pKa = 10.27 LKK34 pKa = 10.66 KK35 pKa = 10.37 YY36 pKa = 6.92 VTEE39 pKa = 4.26 RR40 pKa = 11.84 GKK42 pKa = 10.15 ILPRR46 pKa = 11.84 RR47 pKa = 11.84 ISGNCAIHH55 pKa = 5.88 QRR57 pKa = 11.84 KK58 pKa = 7.58 LTIAIKK64 pKa = 10.18 RR65 pKa = 11.84 ARR67 pKa = 11.84 QIAILPYY74 pKa = 8.74 TTDD77 pKa = 2.74
Molecular weight: 8.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.853
IPC_protein 10.511
Toseland 10.906
ProMoST 10.526
Dawson 10.979
Bjellqvist 10.657
Wikipedia 11.155
Rodwell 11.345
Grimsley 11.008
Solomon 11.082
Lehninger 11.067
Nozaki 10.891
DTASelect 10.643
Thurlkill 10.891
EMBOSS 11.301
Sillero 10.906
Patrickios 11.082
IPC_peptide 11.096
IPC2_peptide 9.677
IPC2.peptide.svr19 8.484
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2839
0
2839
845889
44
1892
298.0
33.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.982 ± 0.041
0.958 ± 0.017
5.814 ± 0.042
7.788 ± 0.056
4.035 ± 0.034
6.39 ± 0.051
1.374 ± 0.019
10.536 ± 0.054
8.97 ± 0.049
9.142 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.554 ± 0.021
5.947 ± 0.041
2.726 ± 0.026
2.419 ± 0.019
3.569 ± 0.027
6.755 ± 0.036
5.018 ± 0.035
6.439 ± 0.043
0.638 ± 0.013
3.947 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here