Alistipes sp. CAG:831

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Rikenellaceae; Alistipes; environmental samples

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2179 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5IIS7|R5IIS7_9BACT UDP-glucose:polyglycerol phosphate glucosyltransferase OS=Alistipes sp. CAG:831 OX=1262698 GN=BN796_00630 PE=4 SV=1
MM1 pKa = 7.17NFKK4 pKa = 10.55PFFAILAAMPLVFTGCHH21 pKa = 6.54PEE23 pKa = 3.79QDD25 pKa = 3.92PPVITAEE32 pKa = 4.39SITVSADD39 pKa = 2.8AHH41 pKa = 6.33SDD43 pKa = 2.88KK44 pKa = 10.48LTYY47 pKa = 10.55SIEE50 pKa = 4.14NPLEE54 pKa = 4.15GEE56 pKa = 4.32TIKK59 pKa = 11.21AEE61 pKa = 4.29SNQDD65 pKa = 3.26WIHH68 pKa = 6.55SFDD71 pKa = 3.72YY72 pKa = 10.24STEE75 pKa = 3.74NTINFEE81 pKa = 3.63VDD83 pKa = 3.04ANISADD89 pKa = 3.49PRR91 pKa = 11.84TATLTLSYY99 pKa = 8.46PTAQSITVNVEE110 pKa = 3.19QMAAGEE116 pKa = 4.44SIALNPEE123 pKa = 3.89NLSFLPEE130 pKa = 4.27GGEE133 pKa = 4.02LSVTVNSGRR142 pKa = 11.84SWVLTGSSDD151 pKa = 2.95WVEE154 pKa = 4.02PSIEE158 pKa = 4.21TGDD161 pKa = 3.72PGAEE165 pKa = 3.81VVFTAKK171 pKa = 10.53ANEE174 pKa = 3.97TDD176 pKa = 3.58EE177 pKa = 4.25PRR179 pKa = 11.84EE180 pKa = 3.76ASFEE184 pKa = 4.47FICGTNKK191 pKa = 8.32VTLNVQQSFAGRR203 pKa = 11.84ILVEE207 pKa = 4.16KK208 pKa = 9.3PQYY211 pKa = 10.1DD212 pKa = 3.36IPGTANTFTVNIQSNMEE229 pKa = 4.2DD230 pKa = 3.31VTVEE234 pKa = 3.91IPEE237 pKa = 4.36DD238 pKa = 3.86ATWLTEE244 pKa = 4.04VTTKK248 pKa = 10.97AMQDD252 pKa = 3.14MAFEE256 pKa = 4.4FSATEE261 pKa = 3.9NEE263 pKa = 4.48GEE265 pKa = 4.27EE266 pKa = 4.17TRR268 pKa = 11.84TAIIVFSNADD278 pKa = 3.0ATEE281 pKa = 3.88QVLISQEE288 pKa = 3.94PSFPADD294 pKa = 3.22VLTKK298 pKa = 10.23IEE300 pKa = 4.71DD301 pKa = 3.86EE302 pKa = 4.32TFKK305 pKa = 10.74TYY307 pKa = 10.53LQEE310 pKa = 5.08NFDD313 pKa = 3.99TDD315 pKa = 3.31GDD317 pKa = 4.19GTINRR322 pKa = 11.84AEE324 pKa = 4.2ADD326 pKa = 3.5AVTSITLTGSEE337 pKa = 4.22MMSAAGIEE345 pKa = 4.18YY346 pKa = 10.28FEE348 pKa = 4.43NLEE351 pKa = 4.72SIDD354 pKa = 3.59FTSNVLLSEE363 pKa = 4.35LSMAGRR369 pKa = 11.84TGLKK373 pKa = 9.57NLNVNSCIRR382 pKa = 11.84LEE384 pKa = 4.11TLDD387 pKa = 3.68LTGCEE392 pKa = 4.17GLEE395 pKa = 3.93TLNIGLCSALTSVDD409 pKa = 3.9LSDD412 pKa = 3.38MTALNTLTAYY422 pKa = 10.62SSGLTSIDD430 pKa = 3.15VTANTQLVALNVNGTGITTVDD451 pKa = 3.69LSQNTEE457 pKa = 4.12LVSLSLTNEE466 pKa = 4.01FSSIDD471 pKa = 3.4LSANTKK477 pKa = 10.5LEE479 pKa = 3.97SLTVGGANLTSLDD492 pKa = 3.78LSANTALRR500 pKa = 11.84EE501 pKa = 4.18LNVSNSGFVSLDD513 pKa = 2.97HH514 pKa = 7.1SMCPDD519 pKa = 4.2LTRR522 pKa = 11.84VTYY525 pKa = 9.57DD526 pKa = 3.05YY527 pKa = 11.09CSNLTTIDD535 pKa = 3.28VSKK538 pKa = 8.75NTRR541 pKa = 11.84MSYY544 pKa = 10.16ISCYY548 pKa = 8.7MCNALTTVIMYY559 pKa = 8.32EE560 pKa = 4.19GQQVSSTFGVSDD572 pKa = 3.64EE573 pKa = 4.39MIQYY577 pKa = 10.08VPFDD581 pKa = 3.9YY582 pKa = 10.84PDD584 pKa = 4.32DD585 pKa = 4.23LSAGIKK591 pKa = 10.22DD592 pKa = 3.61EE593 pKa = 4.0NLRR596 pKa = 11.84KK597 pKa = 10.18YY598 pKa = 10.36ILNLYY603 pKa = 9.33DD604 pKa = 3.61YY605 pKa = 9.81NYY607 pKa = 9.91STGEE611 pKa = 3.69VDD613 pKa = 3.89GKK615 pKa = 10.71IDD617 pKa = 3.42AEE619 pKa = 3.95EE620 pKa = 4.07AARR623 pKa = 11.84VTSLDD628 pKa = 3.15LSGRR632 pKa = 11.84GDD634 pKa = 3.39IVSLTGLDD642 pKa = 3.53YY643 pKa = 9.13FTNLEE648 pKa = 4.16TVNASEE654 pKa = 4.32NMIEE658 pKa = 4.04EE659 pKa = 3.89VDD661 pKa = 4.16FFFNTKK667 pKa = 9.94LKK669 pKa = 10.29EE670 pKa = 4.18VNLSGNKK677 pKa = 8.38LTTIDD682 pKa = 3.73FSKK685 pKa = 10.86NQEE688 pKa = 3.81ITNLDD693 pKa = 3.35LSYY696 pKa = 11.35NQLTSVDD703 pKa = 4.11NLPDD707 pKa = 3.55AVVDD711 pKa = 5.29LDD713 pKa = 4.28LSNNKK718 pKa = 8.26LTSVEE723 pKa = 4.14ASYY726 pKa = 11.32LSNLRR731 pKa = 11.84DD732 pKa = 3.52LNISYY737 pKa = 10.93NEE739 pKa = 3.75IVEE742 pKa = 4.38CDD744 pKa = 3.36VHH746 pKa = 7.95YY747 pKa = 11.09DD748 pKa = 3.43QALVNFDD755 pKa = 4.02CSHH758 pKa = 5.83NQIEE762 pKa = 4.77QINVWTLTALEE773 pKa = 4.59NFNISNNPLLVIGTYY788 pKa = 7.61YY789 pKa = 10.61TNPNGTFFEE798 pKa = 4.45PQLNYY803 pKa = 9.4LTALLSLNCSSTQVSEE819 pKa = 4.65LDD821 pKa = 3.83LSACLLLQQLIATDD835 pKa = 4.14CPEE838 pKa = 4.39LKK840 pKa = 10.51DD841 pKa = 3.81VFIAPGQTIADD852 pKa = 3.92MQLDD856 pKa = 3.94DD857 pKa = 5.33DD858 pKa = 4.76SVVKK862 pKa = 10.7EE863 pKa = 4.29KK864 pKa = 10.49TPEE867 pKa = 3.69TAEE870 pKa = 3.93

Molecular weight: 95.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5IFD4|R5IFD4_9BACT AAA-ATPase OS=Alistipes sp. CAG:831 OX=1262698 GN=BN796_02079 PE=4 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.39KK6 pKa = 9.62RR7 pKa = 11.84KK8 pKa = 6.72RR9 pKa = 11.84HH10 pKa = 5.17KK11 pKa = 10.05MATHH15 pKa = 6.02KK16 pKa = 10.34RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.2NRR25 pKa = 11.84HH26 pKa = 4.71KK27 pKa = 10.86KK28 pKa = 9.47RR29 pKa = 3.41

Molecular weight: 3.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2179

0

2179

759939

29

1717

348.8

38.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.142 ± 0.054

1.269 ± 0.023

5.926 ± 0.035

6.534 ± 0.042

4.234 ± 0.028

7.554 ± 0.045

1.772 ± 0.026

6.66 ± 0.051

4.896 ± 0.051

9.047 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.815 ± 0.022

4.019 ± 0.037

4.031 ± 0.033

2.909 ± 0.028

5.708 ± 0.051

6.698 ± 0.046

5.512 ± 0.04

6.977 ± 0.037

1.158 ± 0.021

4.137 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski