Rotavirus H
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U2XNN9|A0A0U2XNN9_9REOV Intermediate capsid protein VP6 OS=Rotavirus H OX=1348384 PE=3 SV=1
MM1 pKa = 7.57 SDD3 pKa = 3.24 VPRR6 pKa = 11.84 FDD8 pKa = 4.66 LRR10 pKa = 11.84 SKK12 pKa = 10.93 RR13 pKa = 11.84 KK14 pKa = 5.54 TQKK17 pKa = 9.38 KK18 pKa = 8.25 QKK20 pKa = 10.39 VDD22 pKa = 3.28 IFDD25 pKa = 4.36 EE26 pKa = 4.48 DD27 pKa = 4.13 DD28 pKa = 3.99 KK29 pKa = 11.62 EE30 pKa = 5.11 SSIQVDD36 pKa = 4.07 GEE38 pKa = 4.3 SDD40 pKa = 3.61 SLASADD46 pKa = 3.44 TSSNHH51 pKa = 6.35 SYY53 pKa = 11.12 EE54 pKa = 5.07 DD55 pKa = 3.49 YY56 pKa = 11.26 SKK58 pKa = 11.08 AYY60 pKa = 9.65 KK61 pKa = 10.4 DD62 pKa = 3.68 LTLEE66 pKa = 4.29 TPIEE70 pKa = 3.98 QPIGDD75 pKa = 4.77 DD76 pKa = 3.71 DD77 pKa = 4.14 QVDD80 pKa = 4.01 SVSTILEE87 pKa = 4.3 SKK89 pKa = 10.42 VDD91 pKa = 3.59 NSWYY95 pKa = 10.27 DD96 pKa = 3.22 KK97 pKa = 10.62 TVEE100 pKa = 4.11 AEE102 pKa = 4.0 EE103 pKa = 4.31 RR104 pKa = 11.84 VQKK107 pKa = 8.38 XKK109 pKa = 10.8 KK110 pKa = 5.13 XTKK113 pKa = 10.16 QKK115 pKa = 10.25 VQQIDD120 pKa = 3.82 EE121 pKa = 4.37 DD122 pKa = 4.17 TSDD125 pKa = 5.23 LKK127 pKa = 11.36 LQLAQLSLRR136 pKa = 11.84 IQKK139 pKa = 8.92 MEE141 pKa = 3.84 SEE143 pKa = 4.5 TRR145 pKa = 11.84 AKK147 pKa = 10.04 TIDD150 pKa = 3.53 SAYY153 pKa = 9.19 HH154 pKa = 5.64 TIITQAEE161 pKa = 4.42 NLTTPQKK168 pKa = 10.61 KK169 pKa = 10.11 SLIAAIIATMRR180 pKa = 3.55
Molecular weight: 20.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.947
IPC2_protein 4.889
IPC_protein 4.8
Toseland 4.647
ProMoST 4.927
Dawson 4.762
Bjellqvist 4.914
Wikipedia 4.66
Rodwell 4.647
Grimsley 4.558
Solomon 4.762
Lehninger 4.724
Nozaki 4.876
DTASelect 5.08
Thurlkill 4.66
EMBOSS 4.673
Sillero 4.927
Patrickios 4.444
IPC_peptide 4.762
IPC2_peptide 4.914
IPC2.peptide.svr19 4.938
Protein with the highest isoelectric point:
>tr|A0A0U2W5J6|A0A0U2W5J6_9REOV Viral protein 4 OS=Rotavirus H OX=1348384 PE=4 SV=1
MM1 pKa = 8.15 DD2 pKa = 5.07 GANYY6 pKa = 9.77 LAWLARR12 pKa = 11.84 DD13 pKa = 3.39 IVRR16 pKa = 11.84 NLSYY20 pKa = 10.23 TALVYY25 pKa = 10.85 NNPKK29 pKa = 9.62 VAIVTLQDD37 pKa = 3.37 SKK39 pKa = 11.87 EE40 pKa = 3.91 EE41 pKa = 4.22 FISFEE46 pKa = 4.29 KK47 pKa = 9.77 EE48 pKa = 3.53 QKK50 pKa = 9.04 TPNEE54 pKa = 4.8 LISEE58 pKa = 4.16 IQSIVEE64 pKa = 4.27 SNLSIEE70 pKa = 4.82 DD71 pKa = 4.07 KK72 pKa = 10.92 INNLLTIRR80 pKa = 11.84 YY81 pKa = 8.36 ISVYY85 pKa = 10.67 VDD87 pKa = 3.43 DD88 pKa = 5.87 KK89 pKa = 10.99 SDD91 pKa = 3.09 KK92 pKa = 10.72 RR93 pKa = 11.84 EE94 pKa = 3.91 IVSRR98 pKa = 11.84 LLDD101 pKa = 4.16 KK102 pKa = 10.77 IISTLEE108 pKa = 3.89 SKK110 pKa = 10.62 ISISEE115 pKa = 4.09 KK116 pKa = 10.12 LQKK119 pKa = 10.47 CINDD123 pKa = 3.12 IRR125 pKa = 11.84 NEE127 pKa = 3.98 AKK129 pKa = 9.53 SWRR132 pKa = 11.84 IKK134 pKa = 10.61 NSSSFKK140 pKa = 8.53 XYY142 pKa = 9.87 HH143 pKa = 6.0 YY144 pKa = 10.73 NQLISDD150 pKa = 4.55 FIKK153 pKa = 10.73 YY154 pKa = 10.4 NEE156 pKa = 3.94 FEE158 pKa = 4.01 ILEE161 pKa = 4.68 DD162 pKa = 3.53 KK163 pKa = 11.23 DD164 pKa = 3.47 EE165 pKa = 4.28 YY166 pKa = 10.62 RR167 pKa = 11.84 WKK169 pKa = 10.87 SDD171 pKa = 3.02 TLQGLNPNYY180 pKa = 10.01 NHH182 pKa = 6.79 RR183 pKa = 11.84 THH185 pKa = 6.5 TLISSIIFAVQSRR198 pKa = 11.84 ISRR201 pKa = 11.84 YY202 pKa = 8.87 NDD204 pKa = 2.99 EE205 pKa = 4.12 QLEE208 pKa = 4.24 VLLFMFCKK216 pKa = 10.02 IRR218 pKa = 11.84 EE219 pKa = 4.36 CYY221 pKa = 9.5 NDD223 pKa = 4.35 GYY225 pKa = 11.58 LEE227 pKa = 4.31 LLPNRR232 pKa = 11.84 KK233 pKa = 8.54 WSHH236 pKa = 5.4 TIKK239 pKa = 10.83 DD240 pKa = 3.41 LRR242 pKa = 11.84 NNKK245 pKa = 8.88 KK246 pKa = 9.96 IKK248 pKa = 9.58 MYY250 pKa = 9.19 SAKK253 pKa = 9.95 IIHH256 pKa = 6.37 ATCAMISILHH266 pKa = 7.08 ADD268 pKa = 4.29 PIDD271 pKa = 4.2 PYY273 pKa = 11.25 FLAQIVTTFEE283 pKa = 4.59 EE284 pKa = 4.94 IPAHH288 pKa = 6.13 AAKK291 pKa = 10.27 QLSSPMTLYY300 pKa = 10.39 IGIAQIKK307 pKa = 8.47 SNIVISTQRR316 pKa = 11.84 AAEE319 pKa = 4.16 SVASVKK325 pKa = 10.7 PNVSRR330 pKa = 11.84 LDD332 pKa = 3.48 EE333 pKa = 4.24 SQITEE338 pKa = 4.15 WNVEE342 pKa = 3.34 MDD344 pKa = 3.63 EE345 pKa = 4.29 YY346 pKa = 11.03 HH347 pKa = 6.58 FKK349 pKa = 11.17 SSILVNMMKK358 pKa = 10.72 ANIYY362 pKa = 9.33 DD363 pKa = 3.64 VPVRR367 pKa = 11.84 TFYY370 pKa = 11.05 KK371 pKa = 10.29 IFNCFSATFHH381 pKa = 6.21 TGHH384 pKa = 6.89 RR385 pKa = 11.84 VDD387 pKa = 4.38 NPQDD391 pKa = 3.96 AIEE394 pKa = 4.35 AQVKK398 pKa = 9.37 VEE400 pKa = 4.01 YY401 pKa = 10.12 TSDD404 pKa = 3.5 VDD406 pKa = 4.35 RR407 pKa = 11.84 EE408 pKa = 4.32 MYY410 pKa = 9.63 DD411 pKa = 2.72 QYY413 pKa = 11.78 YY414 pKa = 10.77 FMLKK418 pKa = 10.36 RR419 pKa = 11.84 MLTDD423 pKa = 3.04 QFAEE427 pKa = 4.23 YY428 pKa = 10.39 AEE430 pKa = 4.43 EE431 pKa = 3.97 MFHH434 pKa = 7.26 RR435 pKa = 11.84 YY436 pKa = 8.84 NSEE439 pKa = 3.66 VTAEE443 pKa = 4.31 SLASMSNSSNGYY455 pKa = 7.81 SRR457 pKa = 11.84 SVKK460 pKa = 10.62 FIDD463 pKa = 5.01 RR464 pKa = 11.84 EE465 pKa = 4.01 IKK467 pKa = 6.98 TTKK470 pKa = 10.6 KK471 pKa = 9.58 MLHH474 pKa = 6.67 LDD476 pKa = 3.84 DD477 pKa = 6.1 DD478 pKa = 4.6 LSKK481 pKa = 11.34 NLDD484 pKa = 3.37 FTNIGEE490 pKa = 4.39 QISKK494 pKa = 9.63 GIPMGTRR501 pKa = 11.84 NVPARR506 pKa = 11.84 QTRR509 pKa = 11.84 GIFILPWQVAAVQHH523 pKa = 6.29 TIAEE527 pKa = 4.18 FLYY530 pKa = 10.6 KK531 pKa = 9.96 KK532 pKa = 10.11 AKK534 pKa = 9.99 KK535 pKa = 10.15 GGFGATFAEE544 pKa = 4.84 AYY546 pKa = 8.25 VAKK549 pKa = 10.32 AATLTYY555 pKa = 10.43 GILAEE560 pKa = 4.34 ATSKK564 pKa = 11.11 ADD566 pKa = 3.65 QLILYY571 pKa = 8.59 TDD573 pKa = 3.83 VSQWDD578 pKa = 3.7 ASQHH582 pKa = 4.09 NTEE585 pKa = 4.88 PYY587 pKa = 9.9 RR588 pKa = 11.84 SAIINAIKK596 pKa = 10.07 EE597 pKa = 3.89 ARR599 pKa = 11.84 TKK601 pKa = 11.05 YY602 pKa = 10.15 KK603 pKa = 10.01 ISYY606 pKa = 7.25 EE607 pKa = 3.97 QEE609 pKa = 3.97 PKK611 pKa = 10.14 VLGMNVLDD619 pKa = 5.15 KK620 pKa = 10.67 MIEE623 pKa = 3.92 IQEE626 pKa = 4.22 ALLNSNLLVEE636 pKa = 4.61 SQGSKK641 pKa = 10.4 RR642 pKa = 11.84 PPLKK646 pKa = 9.94 IRR648 pKa = 11.84 YY649 pKa = 8.39 HH650 pKa = 5.84 GVASGEE656 pKa = 4.15 KK657 pKa = 6.55 TTKK660 pKa = 10.0 IGNSFANVALITTVFNSLTNSLPSIRR686 pKa = 11.84 VNHH689 pKa = 5.66 MRR691 pKa = 11.84 VDD693 pKa = 3.56 GDD695 pKa = 4.26 DD696 pKa = 3.89 NVVTMYY702 pKa = 10.96 AADD705 pKa = 5.0 PINIIQEE712 pKa = 4.39 AIKK715 pKa = 10.07 MKK717 pKa = 9.96 YY718 pKa = 9.29 KK719 pKa = 10.99 AMNARR724 pKa = 11.84 VKK726 pKa = 10.78 ALASYY731 pKa = 9.46 TGLEE735 pKa = 3.81 MAKK738 pKa = 10.13 RR739 pKa = 11.84 FIIGGKK745 pKa = 8.6 IFEE748 pKa = 4.99 RR749 pKa = 11.84 GAISIFTAEE758 pKa = 4.3 RR759 pKa = 11.84 PYY761 pKa = 10.8 GTDD764 pKa = 2.76 QSVQSTTGSLIYY776 pKa = 10.34 SAAVNAYY783 pKa = 9.79 RR784 pKa = 11.84 GFGDD788 pKa = 3.63 EE789 pKa = 4.22 YY790 pKa = 11.15 LEE792 pKa = 4.6 FMLDD796 pKa = 3.37 VLTPPSASIRR806 pKa = 11.84 VTGRR810 pKa = 11.84 LRR812 pKa = 11.84 SLLSPVTLFSTGPISFEE829 pKa = 3.43 ITPYY833 pKa = 10.91 GLGGRR838 pKa = 11.84 MRR840 pKa = 11.84 FFQINRR846 pKa = 11.84 KK847 pKa = 8.77 NMEE850 pKa = 4.09 LFKK853 pKa = 10.77 ILTSSLSISIEE864 pKa = 3.8 PEE866 pKa = 3.67 EE867 pKa = 4.06 IKK869 pKa = 10.68 QYY871 pKa = 10.11 STTPQFKK878 pKa = 10.4 ARR880 pKa = 11.84 TDD882 pKa = 3.32 RR883 pKa = 11.84 MIEE886 pKa = 4.11 SVRR889 pKa = 11.84 ITMKK893 pKa = 10.48 SEE895 pKa = 3.31 ARR897 pKa = 11.84 IITQILVDD905 pKa = 4.0 KK906 pKa = 10.27 EE907 pKa = 4.11 RR908 pKa = 11.84 QKK910 pKa = 9.96 TLGVPNVATTKK921 pKa = 10.27 NRR923 pKa = 11.84 QQVEE927 pKa = 4.42 KK928 pKa = 10.84 ARR930 pKa = 11.84 KK931 pKa = 6.81 TLSAPKK937 pKa = 9.81 EE938 pKa = 4.04 VLKK941 pKa = 10.83 KK942 pKa = 9.49 VSKK945 pKa = 10.46 YY946 pKa = 8.33 YY947 pKa = 9.95 PEE949 pKa = 5.29 EE950 pKa = 3.73 IFHH953 pKa = 6.33 YY954 pKa = 10.24 IISNSKK960 pKa = 8.95 LHH962 pKa = 6.97 RR963 pKa = 11.84 SQNDD967 pKa = 3.04 EE968 pKa = 3.93 MVQVYY973 pKa = 8.41 MNNSLMIKK981 pKa = 10.16 RR982 pKa = 11.84 LQRR985 pKa = 11.84 QLGVRR990 pKa = 11.84 VAEE993 pKa = 4.9 GSHH996 pKa = 5.16 LTKK999 pKa = 9.5 PTNTLFKK1006 pKa = 10.45 LVEE1009 pKa = 3.9 KK1010 pKa = 10.28 HH1011 pKa = 6.31 SPIKK1015 pKa = 10.33 ISPSDD1020 pKa = 3.47 LVKK1023 pKa = 11.07 YY1024 pKa = 9.37 SEE1026 pKa = 4.92 KK1027 pKa = 10.82 YY1028 pKa = 10.66 DD1029 pKa = 3.5 LTTLQGKK1036 pKa = 9.48 KK1037 pKa = 10.11 KK1038 pKa = 10.07 FLYY1041 pKa = 10.59 DD1042 pKa = 3.72 LGISGSEE1049 pKa = 3.42 LRR1051 pKa = 11.84 FYY1053 pKa = 11.3 LNSKK1057 pKa = 10.47 LLMHH1061 pKa = 7.24 DD1062 pKa = 4.58 LLLAKK1067 pKa = 10.02 YY1068 pKa = 9.39 DD1069 pKa = 3.98 KK1070 pKa = 10.88 LYY1072 pKa = 9.67 EE1073 pKa = 4.3 APGFGATQLNSLPLDD1088 pKa = 3.64 LTAAEE1093 pKa = 4.67 KK1094 pKa = 10.95 VFSIKK1099 pKa = 10.81 VNMPSAYY1106 pKa = 9.68 YY1107 pKa = 10.29 EE1108 pKa = 4.21 LMMLILLYY1116 pKa = 10.42 EE1117 pKa = 4.09 YY1118 pKa = 11.42 VNFVMFTGQTFTCICLPEE1136 pKa = 4.12 TTIQSANIAKK1146 pKa = 10.12 NVMKK1150 pKa = 10.15 MIDD1153 pKa = 4.2 NIQLDD1158 pKa = 4.1 SVSFQDD1164 pKa = 4.8 IIYY1167 pKa = 10.67
Molecular weight: 133.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.174
IPC2_protein 8.258
IPC_protein 8.112
Toseland 8.682
ProMoST 8.668
Dawson 9.063
Bjellqvist 8.902
Wikipedia 9.238
Rodwell 9.209
Grimsley 9.107
Solomon 9.092
Lehninger 9.048
Nozaki 8.902
DTASelect 8.829
Thurlkill 8.931
EMBOSS 9.18
Sillero 9.077
Patrickios 4.418
IPC_peptide 9.092
IPC2_peptide 7.468
IPC2.peptide.svr19 7.58
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
5693
180
1167
517.5
58.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.622 ± 0.463
1.3 ± 0.389
6.043 ± 0.494
6.06 ± 0.37
4.391 ± 0.282
3.847 ± 0.368
1.704 ± 0.284
8.133 ± 0.278
6.763 ± 0.567
8.115 ± 0.483
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.004 ± 0.205
6.64 ± 0.385
3.952 ± 0.325
4.04 ± 0.236
5.287 ± 0.358
7.009 ± 0.591
6.499 ± 0.496
5.92 ± 0.294
0.738 ± 0.105
3.741 ± 0.517
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here