bacterium A37T11
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4337 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H7WLM1|A0A1H7WLM1_9BACT L-rhamnose mutarotase OS=bacterium A37T11 OX=1855384 GN=SAMN05216436_102260 PE=4 SV=1
MM1 pKa = 7.19 YY2 pKa = 7.77 WTLEE6 pKa = 4.21 LASHH10 pKa = 7.01 LEE12 pKa = 4.08 DD13 pKa = 4.93 APWPATKK20 pKa = 10.47 DD21 pKa = 3.38 EE22 pKa = 5.13 LIDD25 pKa = 3.42 YY26 pKa = 7.68 GIRR29 pKa = 11.84 SGAPVEE35 pKa = 4.53 VIEE38 pKa = 4.56 NLQALEE44 pKa = 4.69 DD45 pKa = 4.28 DD46 pKa = 4.31 GEE48 pKa = 4.31 PYY50 pKa = 11.05 EE51 pKa = 4.65 NIEE54 pKa = 4.81 EE55 pKa = 4.08 IWPDD59 pKa = 3.85 YY60 pKa = 7.47 PTKK63 pKa = 10.94 DD64 pKa = 3.05 DD65 pKa = 4.15 FFFNEE70 pKa = 4.51 DD71 pKa = 3.17 EE72 pKa = 4.46 YY73 pKa = 11.99
Molecular weight: 8.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.818
IPC2_protein 3.592
IPC_protein 3.528
Toseland 3.338
ProMoST 3.694
Dawson 3.516
Bjellqvist 3.681
Wikipedia 3.439
Rodwell 3.363
Grimsley 3.261
Solomon 3.478
Lehninger 3.439
Nozaki 3.643
DTASelect 3.795
Thurlkill 3.401
EMBOSS 3.452
Sillero 3.656
Patrickios 0.693
IPC_peptide 3.478
IPC2_peptide 3.617
IPC2.peptide.svr19 3.629
Protein with the highest isoelectric point:
>tr|A0A1H8E483|A0A1H8E483_9BACT Fusaric acid resistance protein-like OS=bacterium A37T11 OX=1855384 GN=SAMN05216436_11232 PE=4 SV=1
MM1 pKa = 7.92 RR2 pKa = 11.84 RR3 pKa = 11.84 RR4 pKa = 11.84 CAWSRR9 pKa = 11.84 RR10 pKa = 11.84 WMPLSILFKK19 pKa = 9.22 TASIGIMPAGLQATSTRR36 pKa = 11.84 IIAEE40 pKa = 3.97 RR41 pKa = 11.84 CQNLSITVNRR51 pKa = 11.84 CEE53 pKa = 4.13 VLSNICNTAICVYY66 pKa = 9.11 VCSVINGDD74 pKa = 3.88 HH75 pKa = 6.41 LKK77 pKa = 10.87 KK78 pKa = 10.62 GGNYY82 pKa = 6.59 EE83 pKa = 4.14 TSIHH87 pKa = 6.54 SIPGSSDD94 pKa = 2.57 ATATGSIRR102 pKa = 11.84 PAVVGMAGMDD112 pKa = 3.49 PGIDD116 pKa = 3.56 SRR118 pKa = 11.84 AMGAINCTVPKK129 pKa = 10.03 KK130 pKa = 10.59 RR131 pKa = 11.84 RR132 pKa = 11.84 DD133 pKa = 3.41 HH134 pKa = 5.88 PAIRR138 pKa = 11.84 PHH140 pKa = 6.05 CWFNRR145 pKa = 11.84 RR146 pKa = 11.84 RR147 pKa = 11.84 RR148 pKa = 11.84 DD149 pKa = 3.24 QCPDD153 pKa = 2.79 LMSAHH158 pKa = 6.81 RR159 pKa = 11.84 RR160 pKa = 11.84 QFCRR164 pKa = 11.84 RR165 pKa = 11.84 RR166 pKa = 11.84 LAAHH170 pKa = 7.09 AADD173 pKa = 3.51 QKK175 pKa = 11.38 KK176 pKa = 9.76 FYY178 pKa = 11.13
Molecular weight: 19.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.428
IPC_protein 10.058
Toseland 10.496
ProMoST 10.204
Dawson 10.599
Bjellqvist 10.35
Wikipedia 10.804
Rodwell 10.774
Grimsley 10.643
Solomon 10.716
Lehninger 10.687
Nozaki 10.584
DTASelect 10.306
Thurlkill 10.511
EMBOSS 10.906
Sillero 10.555
Patrickios 10.496
IPC_peptide 10.716
IPC2_peptide 9.853
IPC2.peptide.svr19 8.48
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4337
0
4337
1547353
27
2511
356.8
40.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.41 ± 0.03
0.705 ± 0.011
5.417 ± 0.024
5.453 ± 0.037
4.729 ± 0.025
7.009 ± 0.033
1.973 ± 0.021
6.832 ± 0.031
6.305 ± 0.03
9.951 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.162 ± 0.015
5.37 ± 0.04
4.013 ± 0.021
4.08 ± 0.021
4.452 ± 0.021
6.629 ± 0.035
5.599 ± 0.026
6.166 ± 0.024
1.313 ± 0.014
4.431 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here