Beihai rhabdo-like virus 5

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Nyamiviridae; Berhavirus; Echinoderm berhavirus

Average proteome isoelectric point is 7.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KMR4|A0A1L3KMR4_9MONO Uncharacterized protein OS=Beihai rhabdo-like virus 5 OX=1922655 PE=4 SV=1
MM1 pKa = 7.18GHH3 pKa = 7.12DD4 pKa = 4.48LSHH7 pKa = 7.79ISIFSFQKK15 pKa = 10.37ADD17 pKa = 3.44LVQDD21 pKa = 3.45YY22 pKa = 11.28GNVVKK27 pKa = 10.73KK28 pKa = 9.82ATGRR32 pKa = 11.84ARR34 pKa = 11.84TSNMTGNRR42 pKa = 11.84KK43 pKa = 8.02TINTVPYY50 pKa = 9.3IQMPSPLMEE59 pKa = 3.87QAFVCHH65 pKa = 6.77KK66 pKa = 6.96MTLRR70 pKa = 11.84LGEE73 pKa = 5.03EE74 pKa = 3.7EE75 pKa = 4.59AEE77 pKa = 4.26WYY79 pKa = 9.98VPRR82 pKa = 11.84PAGCSDD88 pKa = 3.47AEE90 pKa = 4.48YY91 pKa = 10.91GSSAHH96 pKa = 6.24LKK98 pKa = 10.61LVISWSALEE107 pKa = 4.88PILEE111 pKa = 4.18EE112 pKa = 4.06LRR114 pKa = 11.84GSAEE118 pKa = 3.9QQKK121 pKa = 9.94QLAGAAGCTKK131 pKa = 10.25EE132 pKa = 3.87VLEE135 pKa = 4.04ILRR138 pKa = 11.84GFFLISEE145 pKa = 4.62AEE147 pKa = 4.31VATLWAHH154 pKa = 6.65HH155 pKa = 5.66EE156 pKa = 4.63AIHH159 pKa = 5.81GAAMEE164 pKa = 4.11QGEE167 pKa = 4.5PEE169 pKa = 4.07ATLGGSPGKK178 pKa = 10.11KK179 pKa = 9.15RR180 pKa = 11.84PRR182 pKa = 11.84KK183 pKa = 8.89EE184 pKa = 3.75KK185 pKa = 9.84PQASPEE191 pKa = 3.91QSEE194 pKa = 4.49EE195 pKa = 4.17EE196 pKa = 4.32EE197 pKa = 4.31ATSGPRR203 pKa = 11.84RR204 pKa = 11.84KK205 pKa = 9.82RR206 pKa = 11.84FRR208 pKa = 11.84RR209 pKa = 11.84KK210 pKa = 8.55GTTKK214 pKa = 10.46FGRR217 pKa = 11.84FAAFKK222 pKa = 10.7AKK224 pKa = 10.85YY225 pKa = 9.2EE226 pKa = 4.24DD227 pKa = 4.48ANHH230 pKa = 6.77PMYY233 pKa = 10.29QKK235 pKa = 10.91KK236 pKa = 9.84ILLEE240 pKa = 4.21GADD243 pKa = 3.77RR244 pKa = 11.84EE245 pKa = 4.49IYY247 pKa = 9.72QATIYY252 pKa = 9.93SHH254 pKa = 7.11PNVAFAEE261 pKa = 4.25YY262 pKa = 9.48CYY264 pKa = 11.26AKK266 pKa = 9.43ATQSRR271 pKa = 11.84GVEE274 pKa = 4.17GQKK277 pKa = 10.35KK278 pKa = 5.32EE279 pKa = 3.95TRR281 pKa = 11.84FAMGNFILTCLAAFLPGVLFSNAKK305 pKa = 9.95FDD307 pKa = 3.66DD308 pKa = 4.02RR309 pKa = 11.84QYY311 pKa = 11.85GSYY314 pKa = 10.53QLGPVLKK321 pKa = 9.88EE322 pKa = 3.83GSTTEE327 pKa = 3.79QEE329 pKa = 4.05QYY331 pKa = 10.34EE332 pKa = 4.51WPEE335 pKa = 3.62LTAAASRR342 pKa = 11.84ARR344 pKa = 11.84YY345 pKa = 9.84LEE347 pKa = 3.69ILQIGFYY354 pKa = 10.57AALACVKK361 pKa = 10.52SVTQEE366 pKa = 3.68NLSFLEE372 pKa = 4.09KK373 pKa = 9.94RR374 pKa = 11.84WNAAISQSDD383 pKa = 3.73LTSPSKK389 pKa = 10.16TNDD392 pKa = 2.98FSFDD396 pKa = 3.65PANLISFSTNAGDD409 pKa = 3.9YY410 pKa = 10.59LSKK413 pKa = 10.89SDD415 pKa = 5.43LYY417 pKa = 11.29FLTSSLLAEE426 pKa = 4.16TDD428 pKa = 3.35EE429 pKa = 5.54AEE431 pKa = 4.38TSGSIASCLLKK442 pKa = 10.11QVEE445 pKa = 4.85MICEE449 pKa = 3.95YY450 pKa = 11.24SGMPRR455 pKa = 11.84IKK457 pKa = 10.95LMMNWATRR465 pKa = 11.84FDD467 pKa = 4.16SSAHH471 pKa = 5.14YY472 pKa = 9.53CHH474 pKa = 6.75AVVTEE479 pKa = 4.72IISFLTILQEE489 pKa = 3.91IQAKK493 pKa = 10.14VSTAEE498 pKa = 4.05FPFLRR503 pKa = 11.84LTRR506 pKa = 11.84PALLEE511 pKa = 4.06RR512 pKa = 11.84LSARR516 pKa = 11.84SFPHH520 pKa = 6.54LCFCATMDD528 pKa = 4.78AKK530 pKa = 8.76MTKK533 pKa = 9.91NLAVGYY539 pKa = 9.63AGKK542 pKa = 10.0DD543 pKa = 3.49FPMTMSAKK551 pKa = 10.32RR552 pKa = 11.84LQTLLRR558 pKa = 11.84QKK560 pKa = 10.47IPDD563 pKa = 3.46VGALSEE569 pKa = 4.33VQVAALASLGIQVEE583 pKa = 4.07GDD585 pKa = 3.13IDD587 pKa = 3.9EE588 pKa = 4.99VIQVARR594 pKa = 11.84KK595 pKa = 9.42RR596 pKa = 11.84KK597 pKa = 7.96RR598 pKa = 11.84QLVEE602 pKa = 3.86SDD604 pKa = 3.03SDD606 pKa = 3.49

Molecular weight: 67.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KMP2|A0A1L3KMP2_9MONO Putative nucleoprotein OS=Beihai rhabdo-like virus 5 OX=1922655 PE=4 SV=1
MM1 pKa = 7.51EE2 pKa = 4.52VFLLFILLGNCLGRR16 pKa = 11.84KK17 pKa = 8.98RR18 pKa = 11.84RR19 pKa = 11.84FEE21 pKa = 3.99FMVSIGHH28 pKa = 5.7TEE30 pKa = 3.74QSYY33 pKa = 8.15MCHH36 pKa = 7.48DD37 pKa = 3.18IGKK40 pKa = 9.83LYY42 pKa = 10.3RR43 pKa = 11.84HH44 pKa = 6.59RR45 pKa = 11.84FNRR48 pKa = 11.84MNANPKK54 pKa = 8.84VSFYY58 pKa = 10.66IPEE61 pKa = 4.3SVLGEE66 pKa = 4.05QAAHH70 pKa = 6.79FDD72 pKa = 3.96LDD74 pKa = 3.73SDD76 pKa = 4.16CEE78 pKa = 4.16YY79 pKa = 11.12FPVEE83 pKa = 3.9QSGIPAYY90 pKa = 9.31WFHH93 pKa = 6.67SVQRR97 pKa = 11.84PKK99 pKa = 10.12IQYY102 pKa = 9.08RR103 pKa = 11.84GYY105 pKa = 9.32MLKK108 pKa = 10.22KK109 pKa = 8.72PHH111 pKa = 5.29GTRR114 pKa = 11.84GRR116 pKa = 11.84FKK118 pKa = 10.83HH119 pKa = 5.17QFEE122 pKa = 4.31LFARR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 3.61

Molecular weight: 15.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2812

128

1920

703.0

80.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.504 ± 1.329

2.027 ± 0.283

4.765 ± 0.532

6.508 ± 0.824

4.659 ± 0.559

4.943 ± 0.586

3.058 ± 0.538

5.192 ± 0.233

5.832 ± 0.512

10.242 ± 0.529

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.302

3.734 ± 0.589

4.232 ± 0.315

4.125 ± 0.376

6.472 ± 0.606

7.361 ± 0.944

6.294 ± 0.599

5.583 ± 0.536

0.96 ± 0.194

4.267 ± 0.515
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski