Beet mild yellowing virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 8.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q83739|Q83739_9LUTE Movement protein OS=Beet mild yellowing virus OX=156690 PE=3 SV=1
MM1 pKa = 7.72EE2 pKa = 5.78EE3 pKa = 4.41DD4 pKa = 3.6DD5 pKa = 5.38HH6 pKa = 8.79VGKK9 pKa = 10.09HH10 pKa = 6.17DD11 pKa = 4.24ALSALSQWLWSKK23 pKa = 10.73PLGQHH28 pKa = 6.56NADD31 pKa = 4.12LDD33 pKa = 4.21DD34 pKa = 5.7DD35 pKa = 4.63EE36 pKa = 5.2EE37 pKa = 4.44ATTGQEE43 pKa = 3.96EE44 pKa = 4.7LFLPEE49 pKa = 3.67EE50 pKa = 4.35QVRR53 pKa = 11.84ARR55 pKa = 11.84HH56 pKa = 5.76SFSQKK61 pKa = 8.33TISRR65 pKa = 11.84EE66 pKa = 3.92VPAEE70 pKa = 3.71QSRR73 pKa = 11.84SGRR76 pKa = 11.84VYY78 pKa = 8.74QTARR82 pKa = 11.84HH83 pKa = 5.72SLMEE87 pKa = 4.17CSRR90 pKa = 11.84PTMSIKK96 pKa = 10.46SQWSFWSSSPRR107 pKa = 11.84PLPKK111 pKa = 9.89IPVPSLTSWTHH122 pKa = 4.83TVNSVPFHH130 pKa = 6.27QPLTSSGSQNPAGGHH145 pKa = 4.87LQRR148 pKa = 11.84LTSTGRR154 pKa = 11.84TGMTLPRR161 pKa = 11.84TNSGSSTKK169 pKa = 11.09AMVLHH174 pKa = 7.0RR175 pKa = 4.88

Molecular weight: 19.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q83739|Q83739_9LUTE Movement protein OS=Beet mild yellowing virus OX=156690 PE=3 SV=1
MM1 pKa = 6.49NTVVGRR7 pKa = 11.84RR8 pKa = 11.84TINGRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84PRR17 pKa = 11.84RR18 pKa = 11.84QTRR21 pKa = 11.84RR22 pKa = 11.84AQRR25 pKa = 11.84SQPVVVVQASRR36 pKa = 11.84TTQRR40 pKa = 11.84RR41 pKa = 11.84PRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84GNNRR51 pKa = 11.84TRR53 pKa = 11.84RR54 pKa = 11.84TVSTRR59 pKa = 11.84GTGSSEE65 pKa = 3.84TFVFSKK71 pKa = 11.13DD72 pKa = 3.3NLAGSSSGAITFGPSLSDD90 pKa = 3.41CPAFADD96 pKa = 4.04GMLKK100 pKa = 10.2AYY102 pKa = 10.13HH103 pKa = 6.72EE104 pKa = 4.71YY105 pKa = 9.92KK106 pKa = 10.07ISMVILEE113 pKa = 4.63FVSEE117 pKa = 4.2ASSQNSGSIAYY128 pKa = 9.39EE129 pKa = 3.85LDD131 pKa = 3.43PHH133 pKa = 6.94CKK135 pKa = 10.18LSALSSTINKK145 pKa = 9.79FGITKK150 pKa = 9.19PGRR153 pKa = 11.84RR154 pKa = 11.84AFAASYY160 pKa = 10.82INGADD165 pKa = 3.03WHH167 pKa = 7.2DD168 pKa = 3.62VAKK171 pKa = 10.6DD172 pKa = 3.32QFRR175 pKa = 11.84ILYY178 pKa = 8.84KK179 pKa = 11.0GNGSSSIAGSFRR191 pKa = 11.84ITMKK195 pKa = 10.75CQFHH199 pKa = 6.24NPKK202 pKa = 10.51

Molecular weight: 22.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1272

175

656

318.0

35.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.997 ± 0.672

1.808 ± 0.353

3.223 ± 0.26

5.582 ± 0.692

4.638 ± 0.744

6.368 ± 0.593

2.358 ± 0.79

4.088 ± 0.587

5.896 ± 0.996

8.962 ± 1.469

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.044 ± 0.282

3.931 ± 0.467

5.425 ± 0.58

3.538 ± 0.749

7.469 ± 1.807

11.006 ± 1.428

7.39 ± 0.725

5.031 ± 0.259

1.572 ± 0.344

2.673 ± 0.561
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski