Leptomonas moramango leishbunyavirus
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A191Z3B4|A0A191Z3B4_9VIRU Uncharacterized protein OS=Leptomonas moramango leishbunyavirus OX=1859148 PE=4 SV=1
MM1 pKa = 7.48 RR2 pKa = 11.84 VSFLSLILLLLLMASGYY19 pKa = 11.2 ASDD22 pKa = 5.79 DD23 pKa = 4.18 PYY25 pKa = 11.72 CNTHH29 pKa = 6.13 NCLPDD34 pKa = 3.71 NEE36 pKa = 4.36 PLKK39 pKa = 11.02 LVIPSGLVPDD49 pKa = 4.55 NYY51 pKa = 11.19 LCIFGGTSNFEE62 pKa = 4.13 PHH64 pKa = 7.27 DD65 pKa = 4.86 SIAPEE70 pKa = 3.89 QAKK73 pKa = 9.12 MEE75 pKa = 4.44 YY76 pKa = 9.12 YY77 pKa = 10.47 AKK79 pKa = 10.17 HH80 pKa = 6.43 DD81 pKa = 4.03 AYY83 pKa = 9.96 ICRR86 pKa = 11.84 NTPNVLEE93 pKa = 5.17 ASYY96 pKa = 10.12 KK97 pKa = 9.65 WYY99 pKa = 9.9 MEE101 pKa = 4.08 AGKK104 pKa = 10.06 NLVTVLVDD112 pKa = 3.23 VDD114 pKa = 4.33 RR115 pKa = 11.84 SSSPVVPPTSQIRR128 pKa = 11.84 ASSNYY133 pKa = 10.33 LIDD136 pKa = 3.32 AHH138 pKa = 8.06 FEE140 pKa = 3.8 MGMLNIRR147 pKa = 11.84 EE148 pKa = 4.24 PYY150 pKa = 8.52 QRR152 pKa = 11.84 CRR154 pKa = 11.84 PGPSDD159 pKa = 3.77 WIPRR163 pKa = 11.84 RR164 pKa = 11.84 KK165 pKa = 9.79 SEE167 pKa = 4.41 CIHH170 pKa = 6.0 RR171 pKa = 11.84 QSSYY175 pKa = 10.31 NIGMAVVYY183 pKa = 9.78 PSEE186 pKa = 3.71 ILQNYY191 pKa = 9.44 KK192 pKa = 10.07 IDD194 pKa = 3.64 VDD196 pKa = 3.73 PDD198 pKa = 3.65 GVVISTSKK206 pKa = 11.02 GDD208 pKa = 2.9 WSTAIRR214 pKa = 11.84 AEE216 pKa = 4.01 CANYY220 pKa = 9.97 KK221 pKa = 9.7 GLRR224 pKa = 11.84 RR225 pKa = 11.84 HH226 pKa = 7.16 LIGTTLRR233 pKa = 11.84 IRR235 pKa = 11.84 SKK237 pKa = 11.32 DD238 pKa = 3.39 SLACLITGSRR248 pKa = 11.84 QTLYY252 pKa = 11.07 KK253 pKa = 10.38 DD254 pKa = 2.54 ITMRR258 pKa = 11.84 YY259 pKa = 8.91 AFEE262 pKa = 4.71 SKK264 pKa = 9.3 TGCEE268 pKa = 4.13 TKK270 pKa = 10.77 LKK272 pKa = 10.56 ARR274 pKa = 11.84 QLFDD278 pKa = 2.9 SWYY281 pKa = 9.06 SPSFFLSVGPVDD293 pKa = 4.35 RR294 pKa = 11.84 LPVLYY299 pKa = 9.83 EE300 pKa = 4.02 SCKK303 pKa = 10.75 DD304 pKa = 3.65 NQDD307 pKa = 3.46 GEE309 pKa = 4.47 FFRR312 pKa = 11.84 DD313 pKa = 4.29 DD314 pKa = 3.93 YY315 pKa = 11.47 KK316 pKa = 10.32 QQCYY320 pKa = 10.46 ASS322 pKa = 3.61
Molecular weight: 36.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.978
IPC2_protein 6.16
IPC_protein 6.237
Toseland 6.287
ProMoST 6.59
Dawson 6.62
Bjellqvist 6.59
Wikipedia 6.62
Rodwell 6.62
Grimsley 6.351
Solomon 6.634
Lehninger 6.634
Nozaki 6.898
DTASelect 7.015
Thurlkill 7.044
EMBOSS 7.029
Sillero 7.029
Patrickios 3.478
IPC_peptide 6.649
IPC2_peptide 6.825
IPC2.peptide.svr19 6.764
Protein with the highest isoelectric point:
>tr|A0A191Z3B4|A0A191Z3B4_9VIRU Uncharacterized protein OS=Leptomonas moramango leishbunyavirus OX=1859148 PE=4 SV=1
MM1 pKa = 7.41 TSVFLPFDD9 pKa = 4.16 LEE11 pKa = 4.1 LTNALEE17 pKa = 4.4 YY18 pKa = 10.82 KK19 pKa = 10.26 GVSPFEE25 pKa = 4.01 TRR27 pKa = 11.84 TKK29 pKa = 10.08 IVAAGKK35 pKa = 9.19 EE36 pKa = 4.19 NQGKK40 pKa = 9.07 LVALMVACRR49 pKa = 11.84 GTNIFKK55 pKa = 10.49 IVTKK59 pKa = 10.71 AKK61 pKa = 10.66 DD62 pKa = 3.26 PALSTKK68 pKa = 9.79 IVAISEE74 pKa = 4.04 EE75 pKa = 4.25 LSRR78 pKa = 11.84 EE79 pKa = 4.05 FAVSLAHH86 pKa = 6.88 IASAFPEE93 pKa = 4.41 VVYY96 pKa = 8.57 DD97 pKa = 3.57 TRR99 pKa = 11.84 IKK101 pKa = 10.0 ISSSISVNTLAFLKK115 pKa = 10.85 HH116 pKa = 6.3 NGLTHH121 pKa = 6.58 EE122 pKa = 4.46 KK123 pKa = 9.24 WLLANEE129 pKa = 4.43 EE130 pKa = 4.06 FCKK133 pKa = 10.73 LVGLDD138 pKa = 3.54 FAKK141 pKa = 10.57 FLAVEE146 pKa = 3.96 EE147 pKa = 4.99 VIWKK151 pKa = 9.97 DD152 pKa = 3.28 DD153 pKa = 3.65 SFEE156 pKa = 5.03 KK157 pKa = 10.51 IVGARR162 pKa = 11.84 KK163 pKa = 8.94 PLL165 pKa = 3.58
Molecular weight: 18.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.783
IPC2_protein 6.985
IPC_protein 7.337
Toseland 7.176
ProMoST 7.629
Dawson 7.951
Bjellqvist 7.746
Wikipedia 7.892
Rodwell 7.922
Grimsley 7.176
Solomon 8.2
Lehninger 8.214
Nozaki 8.126
DTASelect 7.966
Thurlkill 8.039
EMBOSS 8.229
Sillero 8.287
Patrickios 4.978
IPC_peptide 8.185
IPC2_peptide 7.132
IPC2.peptide.svr19 7.59
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2466
165
1979
822.0
93.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.799 ± 1.044
1.906 ± 0.406
5.231 ± 0.452
6.529 ± 0.536
4.704 ± 0.488
4.461 ± 0.137
2.96 ± 0.519
6.772 ± 0.277
6.042 ± 0.771
9.976 ± 0.271
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.149 ± 0.197
4.623 ± 0.284
4.582 ± 0.455
3.487 ± 0.748
5.069 ± 0.443
9.611 ± 0.517
5.353 ± 0.269
5.474 ± 0.878
1.703 ± 0.22
3.569 ± 0.727
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here