Microbacterium phage Stormbreaker

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Eekayvirinae; Tinytimothyvirus; unclassified Tinytimothyvirus

Average proteome isoelectric point is 5.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9A0E6|A0A7G9A0E6_9CAUD Cas4 family exonuclease OS=Microbacterium phage Stormbreaker OX=2759462 GN=12 PE=4 SV=1
MM1 pKa = 7.49SEE3 pKa = 3.68YY4 pKa = 11.28AEE6 pKa = 4.24AFKK9 pKa = 11.12AQDD12 pKa = 3.39EE13 pKa = 4.54YY14 pKa = 12.02AEE16 pKa = 4.26VQSFSDD22 pKa = 4.32DD23 pKa = 3.58PYY25 pKa = 10.44PYY27 pKa = 10.24YY28 pKa = 10.37FAYY31 pKa = 10.69LRR33 pKa = 11.84FSEE36 pKa = 4.91GKK38 pKa = 9.41LQAAVTRR45 pKa = 11.84DD46 pKa = 3.83DD47 pKa = 4.21FIGWMDD53 pKa = 3.65YY54 pKa = 9.22VHH56 pKa = 6.84EE57 pKa = 4.35EE58 pKa = 4.3WACSLADD65 pKa = 4.09TLDD68 pKa = 3.83TPLKK72 pKa = 9.89EE73 pKa = 4.54FKK75 pKa = 10.69LLEE78 pKa = 4.21YY79 pKa = 10.57LDD81 pKa = 4.22GVDD84 pKa = 3.29WVMPDD89 pKa = 4.13TIHH92 pKa = 7.68DD93 pKa = 4.5GSPHH97 pKa = 6.12PKK99 pKa = 9.98AGEE102 pKa = 4.06VQVDD106 pKa = 2.8NGMYY110 pKa = 9.8EE111 pKa = 3.85WFKK114 pKa = 10.65TLVTNDD120 pKa = 2.64VDD122 pKa = 4.43ACKK125 pKa = 10.43AWLAEE130 pKa = 3.89QGTKK134 pKa = 10.37HH135 pKa = 5.28EE136 pKa = 4.44WEE138 pKa = 4.16VVYY141 pKa = 11.12YY142 pKa = 10.21DD143 pKa = 3.93GPEE146 pKa = 3.57VSYY149 pKa = 11.1EE150 pKa = 4.36KK151 pKa = 10.21IQQDD155 pKa = 4.19IITMLEE161 pKa = 4.33GYY163 pKa = 10.82ADD165 pKa = 3.9DD166 pKa = 4.08SEE168 pKa = 6.03GYY170 pKa = 9.96GFEE173 pKa = 4.06EE174 pKa = 4.08ATVILKK180 pKa = 10.19RR181 pKa = 11.84YY182 pKa = 9.21KK183 pKa = 10.53EE184 pKa = 4.03KK185 pKa = 11.36LNGG188 pKa = 3.49

Molecular weight: 21.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9A0I8|A0A7G9A0I8_9CAUD Uncharacterized protein OS=Microbacterium phage Stormbreaker OX=2759462 GN=54 PE=4 SV=1
MM1 pKa = 7.66PLDD4 pKa = 3.31RR5 pKa = 11.84HH6 pKa = 5.48KK7 pKa = 10.88SIRR10 pKa = 11.84QALSYY15 pKa = 10.43VDD17 pKa = 5.64RR18 pKa = 11.84YY19 pKa = 10.27PDD21 pKa = 3.13WPEE24 pKa = 3.42TARR27 pKa = 11.84IDD29 pKa = 3.61MPIWEE34 pKa = 4.4LVARR38 pKa = 11.84NLFDD42 pKa = 4.19IANHH46 pKa = 6.68PNTKK50 pKa = 9.78VRR52 pKa = 11.84GSLARR57 pKa = 11.84ATRR60 pKa = 11.84AQRR63 pKa = 11.84ILLDD67 pKa = 3.55RR68 pKa = 11.84LTGTRR73 pKa = 11.84RR74 pKa = 11.84QGTNPAVRR82 pKa = 11.84NAKK85 pKa = 9.84SLQLLDD91 pKa = 3.6LTIGLTEE98 pKa = 4.21STEE101 pKa = 4.08GTTDD105 pKa = 2.97EE106 pKa = 5.2RR107 pKa = 11.84SDD109 pKa = 3.71EE110 pKa = 4.14RR111 pKa = 11.84GKK113 pKa = 10.87APQRR117 pKa = 11.84EE118 pKa = 4.3AA119 pKa = 3.72

Molecular weight: 13.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

17128

30

4484

317.2

35.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.575 ± 0.378

0.49 ± 0.151

6.778 ± 0.19

6.574 ± 0.385

3.468 ± 0.141

7.677 ± 0.355

1.617 ± 0.188

4.636 ± 0.194

4.437 ± 0.35

8.337 ± 0.382

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.721 ± 0.128

4.42 ± 0.323

4.723 ± 0.275

4.583 ± 0.191

5.979 ± 0.439

5.938 ± 0.27

6.475 ± 0.381

6.592 ± 0.33

1.716 ± 0.112

3.264 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski