Microscilla marina ATCC 23134

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Microscillaceae; Microscilla; Microscilla marina

Average proteome isoelectric point is 7.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8248 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1ZUU1|A1ZUU1_9BACT Uncharacterized protein OS=Microscilla marina ATCC 23134 OX=313606 GN=M23134_07657 PE=4 SV=1
MM1 pKa = 7.51HH2 pKa = 7.49LFIPQGDD9 pKa = 4.78FSPAIYY15 pKa = 9.38WDD17 pKa = 3.56YY18 pKa = 11.27TIIRR22 pKa = 11.84LDD24 pKa = 3.28IRR26 pKa = 11.84GEE28 pKa = 4.07STMEE32 pKa = 3.87NPQDD36 pKa = 4.47FYY38 pKa = 12.17AHH40 pKa = 7.53LIDD43 pKa = 5.65LLQPSLAANPCKK55 pKa = 10.34KK56 pKa = 10.7LEE58 pKa = 4.36LNLDD62 pKa = 4.05LSYY65 pKa = 11.57LNTGSQKK72 pKa = 10.73SLYY75 pKa = 9.88EE76 pKa = 3.84LFEE79 pKa = 4.02FFIDD83 pKa = 3.73QGLDD87 pKa = 3.07AYY89 pKa = 10.07INWWVDD95 pKa = 3.37DD96 pKa = 4.57AEE98 pKa = 6.11DD99 pKa = 4.9DD100 pKa = 4.77YY101 pKa = 11.71EE102 pKa = 4.21LQAIGDD108 pKa = 4.08YY109 pKa = 10.55TCDD112 pKa = 3.71FPAFRR117 pKa = 11.84VRR119 pKa = 11.84VLQRR123 pKa = 11.84AA124 pKa = 3.35

Molecular weight: 14.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1ZVX8|A1ZVX8_9BACT Tetratricopeptide repeat domain protein OS=Microscilla marina ATCC 23134 OX=313606 GN=M23134_00814 PE=4 SV=1
MM1 pKa = 8.08RR2 pKa = 11.84YY3 pKa = 8.61FALKK7 pKa = 8.41ITQASPYY14 pKa = 10.32EE15 pKa = 4.42GTCLFLIITLAGARR29 pKa = 11.84RR30 pKa = 11.84PRR32 pKa = 11.84VCQHH36 pKa = 6.07RR37 pKa = 11.84QFKK40 pKa = 9.97PKK42 pKa = 9.88KK43 pKa = 7.73QPPRR47 pKa = 11.84VGLEE51 pKa = 3.71ALAA54 pKa = 4.68

Molecular weight: 6.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8248

0

8248

2646701

30

8871

320.9

36.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.194 ± 0.035

0.792 ± 0.011

4.927 ± 0.025

5.808 ± 0.037

4.65 ± 0.024

6.278 ± 0.039

2.179 ± 0.018

6.466 ± 0.023

7.826 ± 0.05

9.803 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.19 ± 0.017

5.473 ± 0.033

3.668 ± 0.018

5.003 ± 0.03

4.059 ± 0.027

5.937 ± 0.026

6.016 ± 0.072

6.401 ± 0.03

1.232 ± 0.01

4.1 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski