Amino acid dipepetide frequency for Brevibacillus phage Davies

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.105AlaAla: 3.105 ± 0.533
0.565AlaCys: 0.565 ± 0.203
3.811AlaAsp: 3.811 ± 0.488
5.082AlaGlu: 5.082 ± 0.614
4.164AlaPhe: 4.164 ± 0.586
3.599AlaGly: 3.599 ± 0.406
0.706AlaHis: 0.706 ± 0.241
4.235AlaIle: 4.235 ± 0.524
6.14AlaLys: 6.14 ± 0.554
6.14AlaLeu: 6.14 ± 0.91
1.906AlaMet: 1.906 ± 0.43
3.388AlaAsn: 3.388 ± 0.484
1.694AlaPro: 1.694 ± 0.468
1.906AlaGln: 1.906 ± 0.355
2.682AlaArg: 2.682 ± 0.448
3.741AlaSer: 3.741 ± 0.498
4.164AlaThr: 4.164 ± 0.824
5.011AlaVal: 5.011 ± 0.714
1.412AlaTrp: 1.412 ± 0.324
2.611AlaTyr: 2.611 ± 0.417
0.0AlaXaa: 0.0 ± 0.0
Cys
0.282CysAla: 0.282 ± 0.178
0.212CysCys: 0.212 ± 0.112
0.282CysAsp: 0.282 ± 0.186
0.917CysGlu: 0.917 ± 0.313
0.494CysPhe: 0.494 ± 0.173
0.212CysGly: 0.212 ± 0.147
0.282CysHis: 0.282 ± 0.115
0.706CysIle: 0.706 ± 0.239
0.282CysLys: 0.282 ± 0.132
0.282CysLeu: 0.282 ± 0.147
0.282CysMet: 0.282 ± 0.126
0.494CysAsn: 0.494 ± 0.184
0.0CysPro: 0.0 ± 0.0
0.282CysGln: 0.282 ± 0.132
0.565CysArg: 0.565 ± 0.214
0.494CysSer: 0.494 ± 0.208
0.282CysThr: 0.282 ± 0.125
0.565CysVal: 0.565 ± 0.179
0.141CysTrp: 0.141 ± 0.151
0.423CysTyr: 0.423 ± 0.152
0.0CysXaa: 0.0 ± 0.0
Asp
3.458AspAla: 3.458 ± 0.442
0.423AspCys: 0.423 ± 0.216
3.67AspAsp: 3.67 ± 0.588
6.07AspGlu: 6.07 ± 0.58
2.4AspPhe: 2.4 ± 0.398
3.952AspGly: 3.952 ± 0.544
0.565AspHis: 0.565 ± 0.212
5.011AspIle: 5.011 ± 0.631
4.446AspLys: 4.446 ± 0.581
4.799AspLeu: 4.799 ± 0.683
1.623AspMet: 1.623 ± 0.268
2.823AspAsn: 2.823 ± 0.49
1.976AspPro: 1.976 ± 0.355
1.835AspGln: 1.835 ± 0.31
2.188AspArg: 2.188 ± 0.292
3.599AspSer: 3.599 ± 0.548
2.752AspThr: 2.752 ± 0.473
3.952AspVal: 3.952 ± 0.556
0.917AspTrp: 0.917 ± 0.258
2.752AspTyr: 2.752 ± 0.362
0.0AspXaa: 0.0 ± 0.0
Glu
5.152GluAla: 5.152 ± 0.529
0.917GluCys: 0.917 ± 0.24
3.529GluAsp: 3.529 ± 0.481
6.917GluGlu: 6.917 ± 0.782
3.176GluPhe: 3.176 ± 0.477
4.164GluGly: 4.164 ± 0.444
1.482GluHis: 1.482 ± 0.267
6.634GluIle: 6.634 ± 0.699
7.199GluLys: 7.199 ± 0.626
6.775GluLeu: 6.775 ± 0.583
2.4GluMet: 2.4 ± 0.459
2.682GluAsn: 2.682 ± 0.374
1.623GluPro: 1.623 ± 0.365
3.741GluGln: 3.741 ± 0.559
5.011GluArg: 5.011 ± 0.644
4.093GluSer: 4.093 ± 0.509
3.176GluThr: 3.176 ± 0.648
6.352GluVal: 6.352 ± 0.65
0.988GluTrp: 0.988 ± 0.303
2.188GluTyr: 2.188 ± 0.381
0.0GluXaa: 0.0 ± 0.0
Phe
2.541PheAla: 2.541 ± 0.382
0.282PheCys: 0.282 ± 0.129
3.458PheAsp: 3.458 ± 0.431
2.964PheGlu: 2.964 ± 0.404
2.188PhePhe: 2.188 ± 0.479
2.611PheGly: 2.611 ± 0.376
0.565PheHis: 0.565 ± 0.246
2.964PheIle: 2.964 ± 0.501
3.247PheLys: 3.247 ± 0.534
4.093PheLeu: 4.093 ± 0.559
0.917PheMet: 0.917 ± 0.233
0.988PheAsn: 0.988 ± 0.305
1.341PhePro: 1.341 ± 0.304
1.27PheGln: 1.27 ± 0.32
1.906PheArg: 1.906 ± 0.397
2.682PheSer: 2.682 ± 0.426
2.047PheThr: 2.047 ± 0.352
3.105PheVal: 3.105 ± 0.462
0.423PheTrp: 0.423 ± 0.149
1.553PheTyr: 1.553 ± 0.339
0.0PheXaa: 0.0 ± 0.0
Gly
3.529GlyAla: 3.529 ± 0.617
0.494GlyCys: 0.494 ± 0.177
3.529GlyAsp: 3.529 ± 0.476
4.164GlyGlu: 4.164 ± 0.533
2.4GlyPhe: 2.4 ± 0.562
2.329GlyGly: 2.329 ± 0.393
0.635GlyHis: 0.635 ± 0.247
3.529GlyIle: 3.529 ± 0.439
5.928GlyLys: 5.928 ± 0.656
4.376GlyLeu: 4.376 ± 0.6
2.258GlyMet: 2.258 ± 0.388
3.882GlyAsn: 3.882 ± 0.487
0.706GlyPro: 0.706 ± 0.202
1.906GlyGln: 1.906 ± 0.414
3.388GlyArg: 3.388 ± 0.472
3.741GlySer: 3.741 ± 0.467
3.529GlyThr: 3.529 ± 0.583
4.87GlyVal: 4.87 ± 0.605
0.847GlyTrp: 0.847 ± 0.292
2.964GlyTyr: 2.964 ± 0.402
0.0GlyXaa: 0.0 ± 0.0
His
1.412HisAla: 1.412 ± 0.347
0.141HisCys: 0.141 ± 0.103
1.129HisAsp: 1.129 ± 0.272
0.917HisGlu: 0.917 ± 0.225
0.988HisPhe: 0.988 ± 0.216
0.917HisGly: 0.917 ± 0.227
0.282HisHis: 0.282 ± 0.172
1.27HisIle: 1.27 ± 0.277
0.635HisLys: 0.635 ± 0.208
1.2HisLeu: 1.2 ± 0.282
0.212HisMet: 0.212 ± 0.098
0.847HisAsn: 0.847 ± 0.244
0.494HisPro: 0.494 ± 0.167
0.494HisGln: 0.494 ± 0.188
0.423HisArg: 0.423 ± 0.18
0.635HisSer: 0.635 ± 0.209
1.129HisThr: 1.129 ± 0.295
0.776HisVal: 0.776 ± 0.316
0.423HisTrp: 0.423 ± 0.169
1.059HisTyr: 1.059 ± 0.322
0.0HisXaa: 0.0 ± 0.0
Ile
5.505IleAla: 5.505 ± 0.595
0.494IleCys: 0.494 ± 0.173
4.305IleAsp: 4.305 ± 0.509
6.422IleGlu: 6.422 ± 0.733
1.764IlePhe: 1.764 ± 0.329
3.952IleGly: 3.952 ± 0.571
1.27IleHis: 1.27 ± 0.274
4.023IleIle: 4.023 ± 0.485
6.493IleLys: 6.493 ± 0.56
5.787IleLeu: 5.787 ± 0.763
1.553IleMet: 1.553 ± 0.373
3.882IleAsn: 3.882 ± 0.518
2.823IlePro: 2.823 ± 0.39
2.682IleGln: 2.682 ± 0.388
3.317IleArg: 3.317 ± 0.377
5.505IleSer: 5.505 ± 0.574
3.952IleThr: 3.952 ± 0.584
4.376IleVal: 4.376 ± 0.483
0.494IleTrp: 0.494 ± 0.167
2.541IleTyr: 2.541 ± 0.461
0.0IleXaa: 0.0 ± 0.0
Lys
5.928LysAla: 5.928 ± 0.711
0.494LysCys: 0.494 ± 0.238
3.458LysAsp: 3.458 ± 0.514
7.269LysGlu: 7.269 ± 0.842
2.541LysPhe: 2.541 ± 0.39
5.999LysGly: 5.999 ± 0.621
1.412LysHis: 1.412 ± 0.293
5.223LysIle: 5.223 ± 0.56
7.34LysLys: 7.34 ± 0.738
6.775LysLeu: 6.775 ± 0.574
2.329LysMet: 2.329 ± 0.43
3.741LysAsn: 3.741 ± 0.547
3.176LysPro: 3.176 ± 0.44
4.164LysGln: 4.164 ± 0.586
4.446LysArg: 4.446 ± 0.649
4.164LysSer: 4.164 ± 0.728
5.576LysThr: 5.576 ± 0.578
5.223LysVal: 5.223 ± 0.569
0.847LysTrp: 0.847 ± 0.198
3.388LysTyr: 3.388 ± 0.507
0.071LysXaa: 0.071 ± 0.077
Leu
7.199LeuAla: 7.199 ± 0.802
0.847LeuCys: 0.847 ± 0.251
6.07LeuAsp: 6.07 ± 0.695
6.422LeuGlu: 6.422 ± 0.839
4.164LeuPhe: 4.164 ± 0.56
4.517LeuGly: 4.517 ± 0.558
1.059LeuHis: 1.059 ± 0.286
5.434LeuIle: 5.434 ± 0.647
6.281LeuLys: 6.281 ± 0.813
6.846LeuLeu: 6.846 ± 0.858
1.482LeuMet: 1.482 ± 0.303
4.305LeuAsn: 4.305 ± 0.567
2.894LeuPro: 2.894 ± 0.378
3.388LeuGln: 3.388 ± 0.48
4.658LeuArg: 4.658 ± 0.648
6.422LeuSer: 6.422 ± 0.663
5.011LeuThr: 5.011 ± 0.635
5.011LeuVal: 5.011 ± 0.761
1.059LeuTrp: 1.059 ± 0.33
2.611LeuTyr: 2.611 ± 0.409
0.0LeuXaa: 0.0 ± 0.0
Met
2.047MetAla: 2.047 ± 0.389
0.071MetCys: 0.071 ± 0.071
1.412MetAsp: 1.412 ± 0.291
1.553MetGlu: 1.553 ± 0.365
1.2MetPhe: 1.2 ± 0.282
2.117MetGly: 2.117 ± 0.359
0.282MetHis: 0.282 ± 0.107
1.482MetIle: 1.482 ± 0.321
3.176MetLys: 3.176 ± 0.492
1.623MetLeu: 1.623 ± 0.322
1.2MetMet: 1.2 ± 0.258
1.694MetAsn: 1.694 ± 0.313
0.565MetPro: 0.565 ± 0.185
1.2MetGln: 1.2 ± 0.267
1.482MetArg: 1.482 ± 0.336
1.835MetSer: 1.835 ± 0.322
1.906MetThr: 1.906 ± 0.311
1.27MetVal: 1.27 ± 0.291
0.0MetTrp: 0.0 ± 0.0
0.635MetTyr: 0.635 ± 0.193
0.0MetXaa: 0.0 ± 0.0
Asn
2.258AsnAla: 2.258 ± 0.645
0.212AsnCys: 0.212 ± 0.128
2.258AsnAsp: 2.258 ± 0.378
2.964AsnGlu: 2.964 ± 0.34
1.835AsnPhe: 1.835 ± 0.316
3.247AsnGly: 3.247 ± 0.395
1.059AsnHis: 1.059 ± 0.269
4.235AsnIle: 4.235 ± 0.56
4.376AsnLys: 4.376 ± 0.622
4.305AsnLeu: 4.305 ± 0.511
1.341AsnMet: 1.341 ± 0.343
3.035AsnAsn: 3.035 ± 0.505
1.482AsnPro: 1.482 ± 0.299
2.117AsnGln: 2.117 ± 0.38
3.035AsnArg: 3.035 ± 0.373
3.035AsnSer: 3.035 ± 0.431
2.682AsnThr: 2.682 ± 0.488
2.823AsnVal: 2.823 ± 0.488
0.988AsnTrp: 0.988 ± 0.254
2.047AsnTyr: 2.047 ± 0.401
0.141AsnXaa: 0.141 ± 0.131
Pro
1.976ProAla: 1.976 ± 0.414
0.282ProCys: 0.282 ± 0.144
1.835ProAsp: 1.835 ± 0.335
1.835ProGlu: 1.835 ± 0.342
1.412ProPhe: 1.412 ± 0.345
0.988ProGly: 0.988 ± 0.244
0.353ProHis: 0.353 ± 0.169
2.188ProIle: 2.188 ± 0.313
2.4ProLys: 2.4 ± 0.464
1.835ProLeu: 1.835 ± 0.292
0.423ProMet: 0.423 ± 0.165
2.047ProAsn: 2.047 ± 0.487
1.2ProPro: 1.2 ± 0.289
1.059ProGln: 1.059 ± 0.254
1.412ProArg: 1.412 ± 0.311
1.976ProSer: 1.976 ± 0.368
2.258ProThr: 2.258 ± 0.464
1.764ProVal: 1.764 ± 0.303
0.565ProTrp: 0.565 ± 0.194
1.129ProTyr: 1.129 ± 0.297
0.0ProXaa: 0.0 ± 0.0
Gln
3.317GlnAla: 3.317 ± 0.475
0.141GlnCys: 0.141 ± 0.107
1.623GlnAsp: 1.623 ± 0.353
2.4GlnGlu: 2.4 ± 0.379
0.776GlnPhe: 0.776 ± 0.219
2.258GlnGly: 2.258 ± 0.368
0.423GlnHis: 0.423 ± 0.162
4.023GlnIle: 4.023 ± 0.525
2.752GlnLys: 2.752 ± 0.4
3.741GlnLeu: 3.741 ± 0.441
1.27GlnMet: 1.27 ± 0.279
2.188GlnAsn: 2.188 ± 0.364
0.847GlnPro: 0.847 ± 0.239
1.976GlnGln: 1.976 ± 0.424
2.117GlnArg: 2.117 ± 0.426
1.835GlnSer: 1.835 ± 0.342
2.47GlnThr: 2.47 ± 0.41
2.823GlnVal: 2.823 ± 0.43
0.353GlnTrp: 0.353 ± 0.154
1.412GlnTyr: 1.412 ± 0.374
0.0GlnXaa: 0.0 ± 0.0
Arg
2.541ArgAla: 2.541 ± 0.38
0.423ArgCys: 0.423 ± 0.174
2.823ArgAsp: 2.823 ± 0.556
4.376ArgGlu: 4.376 ± 0.628
2.188ArgPhe: 2.188 ± 0.329
2.752ArgGly: 2.752 ± 0.525
0.706ArgHis: 0.706 ± 0.272
3.035ArgIle: 3.035 ± 0.476
5.223ArgLys: 5.223 ± 0.672
4.376ArgLeu: 4.376 ± 0.566
2.329ArgMet: 2.329 ± 0.355
1.764ArgAsn: 1.764 ± 0.336
0.988ArgPro: 0.988 ± 0.205
1.976ArgGln: 1.976 ± 0.361
2.47ArgArg: 2.47 ± 0.486
2.258ArgSer: 2.258 ± 0.322
2.682ArgThr: 2.682 ± 0.507
3.317ArgVal: 3.317 ± 0.464
0.776ArgTrp: 0.776 ± 0.209
2.188ArgTyr: 2.188 ± 0.36
0.0ArgXaa: 0.0 ± 0.0
Ser
2.964SerAla: 2.964 ± 0.408
0.212SerCys: 0.212 ± 0.115
4.093SerAsp: 4.093 ± 0.548
4.376SerGlu: 4.376 ± 0.646
3.247SerPhe: 3.247 ± 0.48
3.529SerGly: 3.529 ± 0.362
1.059SerHis: 1.059 ± 0.273
3.952SerIle: 3.952 ± 0.596
4.587SerLys: 4.587 ± 0.56
6.07SerLeu: 6.07 ± 0.639
1.482SerMet: 1.482 ± 0.31
2.611SerAsn: 2.611 ± 0.491
2.258SerPro: 2.258 ± 0.393
2.258SerGln: 2.258 ± 0.37
1.764SerArg: 1.764 ± 0.321
3.67SerSer: 3.67 ± 0.586
3.529SerThr: 3.529 ± 0.434
4.305SerVal: 4.305 ± 0.486
0.635SerTrp: 0.635 ± 0.223
2.47SerTyr: 2.47 ± 0.47
0.0SerXaa: 0.0 ± 0.0
Thr
4.517ThrAla: 4.517 ± 0.725
0.423ThrCys: 0.423 ± 0.19
3.741ThrAsp: 3.741 ± 0.644
4.305ThrGlu: 4.305 ± 0.554
1.835ThrPhe: 1.835 ± 0.358
4.376ThrGly: 4.376 ± 0.652
0.988ThrHis: 0.988 ± 0.247
4.799ThrIle: 4.799 ± 0.663
4.658ThrLys: 4.658 ± 0.55
5.576ThrLeu: 5.576 ± 0.634
0.847ThrMet: 0.847 ± 0.286
2.894ThrAsn: 2.894 ± 0.408
1.553ThrPro: 1.553 ± 0.298
1.623ThrGln: 1.623 ± 0.289
1.835ThrArg: 1.835 ± 0.364
3.176ThrSer: 3.176 ± 0.6
3.529ThrThr: 3.529 ± 0.585
4.658ThrVal: 4.658 ± 0.569
0.847ThrTrp: 0.847 ± 0.218
2.329ThrTyr: 2.329 ± 0.394
0.0ThrXaa: 0.0 ± 0.0
Val
5.364ValAla: 5.364 ± 0.675
0.282ValCys: 0.282 ± 0.155
4.94ValAsp: 4.94 ± 0.542
4.305ValGlu: 4.305 ± 0.616
2.258ValPhe: 2.258 ± 0.508
3.811ValGly: 3.811 ± 0.481
1.129ValHis: 1.129 ± 0.341
4.94ValIle: 4.94 ± 0.557
4.164ValLys: 4.164 ± 0.608
5.787ValLeu: 5.787 ± 0.765
1.906ValMet: 1.906 ± 0.331
3.317ValAsn: 3.317 ± 0.432
2.188ValPro: 2.188 ± 0.356
2.894ValGln: 2.894 ± 0.464
3.247ValArg: 3.247 ± 0.44
3.811ValSer: 3.811 ± 0.441
5.011ValThr: 5.011 ± 0.61
5.928ValVal: 5.928 ± 0.869
0.706ValTrp: 0.706 ± 0.202
3.458ValTyr: 3.458 ± 0.597
0.0ValXaa: 0.0 ± 0.0
Trp
0.423TrpAla: 0.423 ± 0.172
0.141TrpCys: 0.141 ± 0.099
0.494TrpAsp: 0.494 ± 0.193
1.059TrpGlu: 1.059 ± 0.259
0.494TrpPhe: 0.494 ± 0.195
1.059TrpGly: 1.059 ± 0.257
0.141TrpHis: 0.141 ± 0.094
0.706TrpIle: 0.706 ± 0.232
1.694TrpLys: 1.694 ± 0.269
1.553TrpLeu: 1.553 ± 0.361
0.282TrpMet: 0.282 ± 0.127
0.353TrpAsn: 0.353 ± 0.156
0.141TrpPro: 0.141 ± 0.097
0.565TrpGln: 0.565 ± 0.191
1.059TrpArg: 1.059 ± 0.308
0.776TrpSer: 0.776 ± 0.218
0.847TrpThr: 0.847 ± 0.234
0.917TrpVal: 0.917 ± 0.208
0.353TrpTrp: 0.353 ± 0.219
0.141TrpTyr: 0.141 ± 0.102
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.541TyrAla: 2.541 ± 0.392
0.423TyrCys: 0.423 ± 0.162
2.752TyrAsp: 2.752 ± 0.416
3.882TyrGlu: 3.882 ± 0.602
1.623TyrPhe: 1.623 ± 0.329
2.611TyrGly: 2.611 ± 0.506
0.988TyrHis: 0.988 ± 0.289
2.752TyrIle: 2.752 ± 0.446
2.47TyrLys: 2.47 ± 0.476
3.741TyrLeu: 3.741 ± 0.511
0.635TyrMet: 0.635 ± 0.198
2.47TyrAsn: 2.47 ± 0.431
0.988TyrPro: 0.988 ± 0.239
1.412TyrGln: 1.412 ± 0.239
2.188TyrArg: 2.188 ± 0.431
1.623TyrSer: 1.623 ± 0.338
1.976TyrThr: 1.976 ± 0.403
2.4TyrVal: 2.4 ± 0.404
0.423TyrTrp: 0.423 ± 0.158
1.694TyrTyr: 1.694 ± 0.413
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.071XaaGly: 0.071 ± 0.077
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.071XaaLys: 0.071 ± 0.065
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.071XaaAsn: 0.071 ± 0.065
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (14170 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski