Fusobacterium sp. CAG:815
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1820 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R7LW28|R7LW28_9FUSO Putative glycosyl/glycerophosphate transferases involved in teichoic acid biosynthesis TagF/TagB/EpsJ/RodC OS=Fusobacterium sp. CAG:815 OX=1262901 GN=BN791_00451 PE=3 SV=1
MM1 pKa = 7.59 ILLFDD6 pKa = 4.31 SKK8 pKa = 11.08 ASKK11 pKa = 9.0 STASAKK17 pKa = 10.09 SSKK20 pKa = 9.75 VRR22 pKa = 11.84 NNPVEE27 pKa = 3.92 NSGILAMNMNDD38 pKa = 3.3 ARR40 pKa = 11.84 SLLTMGEE47 pKa = 3.9 YY48 pKa = 9.02 DD49 pKa = 3.83 TYY51 pKa = 11.49 VSSNPVAVDD60 pKa = 2.93 YY61 pKa = 11.79 AMYY64 pKa = 10.69 SNSSDD69 pKa = 3.49 FSNSDD74 pKa = 3.29 SGFMSEE80 pKa = 5.36 FSDD83 pKa = 4.84 AVATLTDD90 pKa = 3.42 GGFGGFGGFSAGADD104 pKa = 3.57 CGASCSSGSFASVCC118 pKa = 3.76
Molecular weight: 12.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.821
IPC2_protein 4.088
IPC_protein 4.012
Toseland 3.795
ProMoST 4.202
Dawson 4.012
Bjellqvist 4.177
Wikipedia 3.986
Rodwell 3.834
Grimsley 3.706
Solomon 3.999
Lehninger 3.961
Nozaki 4.139
DTASelect 4.406
Thurlkill 3.872
EMBOSS 3.986
Sillero 4.139
Patrickios 1.964
IPC_peptide 3.999
IPC2_peptide 4.113
IPC2.peptide.svr19 4.017
Protein with the highest isoelectric point:
>tr|R7M0S6|R7M0S6_9FUSO Cell division protein FtsZ OS=Fusobacterium sp. CAG:815 OX=1262901 GN=ftsZ PE=3 SV=1
MM1 pKa = 7.68 RR2 pKa = 11.84 RR3 pKa = 11.84 TLEE6 pKa = 3.61 GTKK9 pKa = 9.97 RR10 pKa = 11.84 KK11 pKa = 8.92 RR12 pKa = 11.84 QNVSGFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTPGGRR28 pKa = 11.84 EE29 pKa = 3.69 VINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 HH40 pKa = 5.36 KK41 pKa = 10.99 LSITAKK47 pKa = 10.1 KK48 pKa = 9.93 RR49 pKa = 11.84 AA50 pKa = 3.48
Molecular weight: 5.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.363
IPC2_protein 10.774
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.193
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.93
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.122
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1820
0
1820
548868
30
1972
301.6
34.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.458 ± 0.063
1.247 ± 0.025
5.769 ± 0.044
6.685 ± 0.07
4.504 ± 0.052
5.779 ± 0.062
1.492 ± 0.019
8.383 ± 0.056
9.167 ± 0.066
8.87 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.523 ± 0.029
6.372 ± 0.064
3.307 ± 0.036
3.309 ± 0.037
3.355 ± 0.034
6.344 ± 0.05
5.551 ± 0.042
6.228 ± 0.049
0.669 ± 0.017
3.988 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here