Fusobacterium sp. CAG:815 
Average proteome isoelectric point is 6.84 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 1820 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|R7LW28|R7LW28_9FUSO Putative glycosyl/glycerophosphate transferases involved in teichoic acid biosynthesis TagF/TagB/EpsJ/RodC OS=Fusobacterium sp. CAG:815 OX=1262901 GN=BN791_00451 PE=3 SV=1MM1 pKa = 7.59  ILLFDD6 pKa = 4.31  SKK8 pKa = 11.08  ASKK11 pKa = 9.0  STASAKK17 pKa = 10.09  SSKK20 pKa = 9.75  VRR22 pKa = 11.84  NNPVEE27 pKa = 3.92  NSGILAMNMNDD38 pKa = 3.3  ARR40 pKa = 11.84  SLLTMGEE47 pKa = 3.9  YY48 pKa = 9.02  DD49 pKa = 3.83  TYY51 pKa = 11.49  VSSNPVAVDD60 pKa = 2.93  YY61 pKa = 11.79  AMYY64 pKa = 10.69  SNSSDD69 pKa = 3.49  FSNSDD74 pKa = 3.29  SGFMSEE80 pKa = 5.36  FSDD83 pKa = 4.84  AVATLTDD90 pKa = 3.42  GGFGGFGGFSAGADD104 pKa = 3.57  CGASCSSGSFASVCC118 pKa = 3.76  
 12.1 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.821 
IPC2_protein 4.088 
IPC_protein 4.012 
Toseland    3.795 
ProMoST     4.202 
Dawson      4.012 
Bjellqvist  4.177 
Wikipedia   3.986 
Rodwell     3.834 
Grimsley    3.706 
Solomon     3.999 
Lehninger   3.961 
Nozaki      4.139 
DTASelect   4.406 
Thurlkill   3.872 
EMBOSS      3.986 
Sillero     4.139 
Patrickios  1.964 
IPC_peptide 3.999 
IPC2_peptide  4.113 
IPC2.peptide.svr19  4.017 
 Protein with the highest isoelectric point: 
>tr|R7M0S6|R7M0S6_9FUSO Cell division protein FtsZ OS=Fusobacterium sp. CAG:815 OX=1262901 GN=ftsZ PE=3 SV=1MM1 pKa = 7.68  RR2 pKa = 11.84  RR3 pKa = 11.84  TLEE6 pKa = 3.61  GTKK9 pKa = 9.97  RR10 pKa = 11.84  KK11 pKa = 8.92  RR12 pKa = 11.84  QNVSGFRR19 pKa = 11.84  ARR21 pKa = 11.84  MSTPGGRR28 pKa = 11.84  EE29 pKa = 3.69  VINRR33 pKa = 11.84  RR34 pKa = 11.84  RR35 pKa = 11.84  ARR37 pKa = 11.84  GRR39 pKa = 11.84  HH40 pKa = 5.36  KK41 pKa = 10.99  LSITAKK47 pKa = 10.1  KK48 pKa = 9.93  RR49 pKa = 11.84  AA50 pKa = 3.48  
 5.83 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.363 
IPC2_protein 10.774 
IPC_protein 12.369 
Toseland    12.53 
ProMoST     13.027 
Dawson      12.53 
Bjellqvist  12.53 
Wikipedia   13.013 
Rodwell     12.193 
Grimsley    12.574 
Solomon     13.027 
Lehninger   12.925 
Nozaki      12.53 
DTASelect   12.53 
Thurlkill   12.53 
EMBOSS      13.027 
Sillero     12.53 
Patrickios  11.93 
IPC_peptide 13.027 
IPC2_peptide  12.018 
IPC2.peptide.svr19  9.122 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        1820 
0
1820 
548868
30
1972
301.6
34.01
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        6.458 ± 0.063
1.247 ± 0.025
5.769 ± 0.044
6.685 ± 0.07
4.504 ± 0.052
5.779 ± 0.062
1.492 ± 0.019
8.383 ± 0.056
9.167 ± 0.066
8.87 ± 0.061
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.523 ± 0.029
6.372 ± 0.064
3.307 ± 0.036
3.309 ± 0.037
3.355 ± 0.034
6.344 ± 0.05
5.551 ± 0.042
6.228 ± 0.049
0.669 ± 0.017
3.988 ± 0.051
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here