Bos taurus papillomavirus 7
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q30BI9|Q30BI9_9PAPI E4 protein OS=Bos taurus papillomavirus 7 OX=1001533 GN=E4 PE=4 SV=1
MM1 pKa = 7.34 SAATMSRR8 pKa = 11.84 SRR10 pKa = 11.84 VKK12 pKa = 10.42 RR13 pKa = 11.84 ASAEE17 pKa = 3.74 DD18 pKa = 4.66 LYY20 pKa = 11.13 RR21 pKa = 11.84 QCQLGADD28 pKa = 4.32 CPPDD32 pKa = 3.46 VKK34 pKa = 11.24 NKK36 pKa = 10.29 FEE38 pKa = 4.92 NNTVADD44 pKa = 5.85 RR45 pKa = 11.84 ILKK48 pKa = 8.41 WVAGFLYY55 pKa = 10.51 LGTLGIGTGRR65 pKa = 11.84 GTGGRR70 pKa = 11.84 GGYY73 pKa = 9.62 VPIGRR78 pKa = 11.84 GPGTTTEE85 pKa = 3.81 IGGTRR90 pKa = 11.84 TLRR93 pKa = 11.84 PVGPVEE99 pKa = 4.42 PIGPGTPTVIDD110 pKa = 3.45 ATPPVDD116 pKa = 4.26 VVEE119 pKa = 4.5 TPIDD123 pKa = 3.55 PTLTDD128 pKa = 3.75 VRR130 pKa = 11.84 PTDD133 pKa = 3.54 PSVFEE138 pKa = 4.81 PGGEE142 pKa = 4.16 DD143 pKa = 3.8 IEE145 pKa = 5.85 LEE147 pKa = 4.14 TLQPEE152 pKa = 4.34 EE153 pKa = 4.59 DD154 pKa = 3.85 VLAGSNPTTDD164 pKa = 3.73 LPTVGEE170 pKa = 4.05 PNIDD174 pKa = 3.51 FTEE177 pKa = 4.27 TSFTEE182 pKa = 3.67 VRR184 pKa = 11.84 PPVSRR189 pKa = 11.84 TADD192 pKa = 3.04 ISEE195 pKa = 4.41 TNLDD199 pKa = 3.37 NAAYY203 pKa = 9.0 NAAVAEE209 pKa = 4.68 FARR212 pKa = 11.84 EE213 pKa = 3.94 ANQVSVIFDD222 pKa = 3.74 AEE224 pKa = 4.29 VGGSVVGSEE233 pKa = 4.0 EE234 pKa = 4.16 FEE236 pKa = 4.44 LEE238 pKa = 4.2 EE239 pKa = 4.61 VPLTSTPEE247 pKa = 3.89 NPARR251 pKa = 11.84 PAGRR255 pKa = 11.84 RR256 pKa = 11.84 RR257 pKa = 11.84 NWGSMYY263 pKa = 10.34 HH264 pKa = 6.76 RR265 pKa = 11.84 FIKK268 pKa = 9.57 QVRR271 pKa = 11.84 LGSTSFSRR279 pKa = 11.84 ADD281 pKa = 3.05 VGGRR285 pKa = 11.84 FEE287 pKa = 4.46 FEE289 pKa = 3.76 NPAFEE294 pKa = 4.98 GDD296 pKa = 3.65 VGVSEE301 pKa = 5.66 EE302 pKa = 4.05 MMQTRR307 pKa = 11.84 DD308 pKa = 3.32 LGEE311 pKa = 3.92 VVIAKK316 pKa = 10.03 GPEE319 pKa = 3.53 GRR321 pKa = 11.84 VRR323 pKa = 11.84 MSRR326 pKa = 11.84 LARR329 pKa = 11.84 IPGMHH334 pKa = 6.05 TRR336 pKa = 11.84 SGLEE340 pKa = 3.87 LGEE343 pKa = 4.41 HH344 pKa = 4.89 VHH346 pKa = 6.71 LFADD350 pKa = 3.8 MSTIEE355 pKa = 4.31 EE356 pKa = 4.53 LPLEE360 pKa = 4.19 EE361 pKa = 4.63 TIEE364 pKa = 4.3 LSTFSNPQGVLDD376 pKa = 4.24 SGPVIIEE383 pKa = 4.12 SEE385 pKa = 3.79 IGATQGVVVNEE396 pKa = 3.96 QTPNPFDD403 pKa = 3.81 NADD406 pKa = 3.66 LGNTVSEE413 pKa = 4.35 TADD416 pKa = 3.57 LLEE419 pKa = 4.56 WGVEE423 pKa = 4.16 DD424 pKa = 5.81 IEE426 pKa = 5.52 LLAQEE431 pKa = 5.39 DD432 pKa = 5.14 YY433 pKa = 11.4 NFTGGRR439 pKa = 11.84 LRR441 pKa = 11.84 LLDD444 pKa = 3.72 VEE446 pKa = 4.47 EE447 pKa = 5.61 APDD450 pKa = 3.58 IDD452 pKa = 4.73 DD453 pKa = 3.72 WTLEE457 pKa = 4.29 SPRR460 pKa = 11.84 KK461 pKa = 9.58 AYY463 pKa = 10.09 AVATINKK470 pKa = 8.89 DD471 pKa = 3.42 SKK473 pKa = 10.97 SQIPVKK479 pKa = 10.45 IPVHH483 pKa = 6.35 VDD485 pKa = 2.92 PSDD488 pKa = 3.67 VVVISYY494 pKa = 8.25 TADD497 pKa = 2.99 VSIFSLFEE505 pKa = 3.74 PSLYY509 pKa = 10.23 RR510 pKa = 11.84 KK511 pKa = 9.69 RR512 pKa = 11.84 KK513 pKa = 9.12 YY514 pKa = 10.63 SYY516 pKa = 10.91 LYY518 pKa = 10.79
Molecular weight: 56.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.547
IPC2_protein 4.418
IPC_protein 4.38
Toseland 4.215
ProMoST 4.469
Dawson 4.317
Bjellqvist 4.469
Wikipedia 4.177
Rodwell 4.202
Grimsley 4.126
Solomon 4.317
Lehninger 4.266
Nozaki 4.418
DTASelect 4.571
Thurlkill 4.215
EMBOSS 4.19
Sillero 4.482
Patrickios 3.986
IPC_peptide 4.317
IPC2_peptide 4.469
IPC2.peptide.svr19 4.422
Protein with the highest isoelectric point:
>tr|Q30BJ1|Q30BJ1_9PAPI Replication protein E1 OS=Bos taurus papillomavirus 7 OX=1001533 GN=E1 PE=3 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.33 KK3 pKa = 10.17 LAEE6 pKa = 4.69 HH7 pKa = 7.16 LDD9 pKa = 3.55 ACQEE13 pKa = 3.92 QMLEE17 pKa = 4.38 LIEE20 pKa = 5.09 KK21 pKa = 10.23 DD22 pKa = 3.68 SQEE25 pKa = 4.81 LEE27 pKa = 4.13 DD28 pKa = 5.97 QIDD31 pKa = 3.44 YY32 pKa = 10.24 WDD34 pKa = 3.59 LVKK37 pKa = 10.65 RR38 pKa = 11.84 EE39 pKa = 3.93 NLLLFAAKK47 pKa = 9.87 EE48 pKa = 4.09 AGLSRR53 pKa = 11.84 LGYY56 pKa = 10.49 EE57 pKa = 4.16 PVPPTKK63 pKa = 10.49 VSEE66 pKa = 4.43 GKK68 pKa = 10.31 AKK70 pKa = 10.22 NAIMMSISLQSLQSSEE86 pKa = 4.36 FGRR89 pKa = 11.84 DD90 pKa = 3.11 PWTLPQTSLEE100 pKa = 4.13 VFMSNPSNCFKK111 pKa = 11.13 KK112 pKa = 10.39 NGEE115 pKa = 4.15 HH116 pKa = 6.81 VEE118 pKa = 4.07 VLFDD122 pKa = 3.77 GDD124 pKa = 3.58 KK125 pKa = 10.03 NKK127 pKa = 10.82 AVIFVKK133 pKa = 9.83 WGEE136 pKa = 4.28 VYY138 pKa = 10.89 VQDD141 pKa = 6.14 LLGAWHH147 pKa = 7.24 KK148 pKa = 10.81 CPSHH152 pKa = 6.32 VVYY155 pKa = 10.72 EE156 pKa = 4.67 GIYY159 pKa = 9.99 YY160 pKa = 8.64 NHH162 pKa = 7.88 PDD164 pKa = 3.44 YY165 pKa = 11.52 GRR167 pKa = 11.84 TFYY170 pKa = 11.18 LRR172 pKa = 11.84 FEE174 pKa = 4.3 EE175 pKa = 4.54 EE176 pKa = 3.58 AAKK179 pKa = 10.81 YY180 pKa = 9.17 GAHH183 pKa = 6.8 KK184 pKa = 9.09 PWQVMTTNGTLLHH197 pKa = 6.51 SPSEE201 pKa = 4.47 SSNSADD207 pKa = 3.32 GSEE210 pKa = 4.41 EE211 pKa = 3.91 SAAPSPGPSIEE222 pKa = 4.0 APQRR226 pKa = 11.84 LSFWGSPAGGPEE238 pKa = 3.43 RR239 pKa = 11.84 GRR241 pKa = 11.84 RR242 pKa = 11.84 RR243 pKa = 11.84 RR244 pKa = 11.84 SEE246 pKa = 4.06 TPRR249 pKa = 11.84 KK250 pKa = 9.69 RR251 pKa = 11.84 SFGDD255 pKa = 3.1 RR256 pKa = 11.84 RR257 pKa = 11.84 PRR259 pKa = 11.84 PQTPLGGLRR268 pKa = 11.84 RR269 pKa = 11.84 KK270 pKa = 9.51 RR271 pKa = 11.84 VRR273 pKa = 11.84 RR274 pKa = 11.84 GRR276 pKa = 11.84 GGGLGVKK283 pKa = 9.66 EE284 pKa = 4.3 LAEE287 pKa = 4.19 KK288 pKa = 10.77 AGGRR292 pKa = 11.84 LAGTPGQTAEE302 pKa = 4.48 GGGHH306 pKa = 6.54 TSTAPGHH313 pKa = 5.44 YY314 pKa = 8.96 PVLIGKK320 pKa = 7.8 GRR322 pKa = 11.84 PNCLKK327 pKa = 10.0 CWRR330 pKa = 11.84 NRR332 pKa = 11.84 FGVSHH337 pKa = 7.07 KK338 pKa = 11.02 GLFLDD343 pKa = 5.17 CSSTFSWTQTGGGRR357 pKa = 11.84 GVDD360 pKa = 3.77 GVILIVFEE368 pKa = 4.75 TEE370 pKa = 3.67 QQLQTFVDD378 pKa = 4.51 TVHH381 pKa = 6.99 RR382 pKa = 11.84 PTSISLRR389 pKa = 11.84 RR390 pKa = 11.84 GGTVLRR396 pKa = 11.84 AGCFF400 pKa = 3.26
Molecular weight: 44.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.454
IPC2_protein 8.331
IPC_protein 8.244
Toseland 8.741
ProMoST 8.77
Dawson 9.136
Bjellqvist 9.136
Wikipedia 9.238
Rodwell 9.282
Grimsley 8.975
Solomon 9.238
Lehninger 9.209
Nozaki 9.311
DTASelect 8.975
Thurlkill 9.092
EMBOSS 9.311
Sillero 9.297
Patrickios 4.876
IPC_peptide 9.238
IPC2_peptide 7.907
IPC2.peptide.svr19 7.694
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2316
50
600
330.9
37.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.915 ± 0.339
2.807 ± 0.957
5.872 ± 0.617
6.995 ± 0.778
4.447 ± 0.351
7.297 ± 1.132
2.159 ± 0.335
3.8 ± 0.369
5.57 ± 0.96
8.981 ± 0.896
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.375 ± 0.39
4.361 ± 0.514
6.002 ± 0.832
3.109 ± 0.361
5.959 ± 0.687
7.254 ± 0.655
6.347 ± 0.696
6.261 ± 0.795
1.382 ± 0.191
3.109 ± 0.455
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here