Amino acid dipepetide frequency for Salinibacillus kushneri

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.591AlaAla: 4.591 ± 0.091
0.535AlaCys: 0.535 ± 0.026
3.147AlaAsp: 3.147 ± 0.056
4.189AlaGlu: 4.189 ± 0.08
3.112AlaPhe: 3.112 ± 0.06
5.005AlaGly: 5.005 ± 0.094
1.211AlaHis: 1.211 ± 0.031
5.546AlaIle: 5.546 ± 0.076
4.54AlaLys: 4.54 ± 0.072
6.435AlaLeu: 6.435 ± 0.088
1.87AlaMet: 1.87 ± 0.048
2.778AlaAsn: 2.778 ± 0.048
1.773AlaPro: 1.773 ± 0.042
2.157AlaGln: 2.157 ± 0.053
2.318AlaArg: 2.318 ± 0.051
3.733AlaSer: 3.733 ± 0.069
3.118AlaThr: 3.118 ± 0.061
4.814AlaVal: 4.814 ± 0.082
0.545AlaTrp: 0.545 ± 0.028
2.162AlaTyr: 2.162 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.348CysAla: 0.348 ± 0.02
0.064CysCys: 0.064 ± 0.009
0.353CysAsp: 0.353 ± 0.019
0.379CysGlu: 0.379 ± 0.019
0.272CysPhe: 0.272 ± 0.018
0.605CysGly: 0.605 ± 0.029
0.196CysHis: 0.196 ± 0.014
0.454CysIle: 0.454 ± 0.023
0.342CysLys: 0.342 ± 0.018
0.585CysLeu: 0.585 ± 0.029
0.169CysMet: 0.169 ± 0.013
0.244CysAsn: 0.244 ± 0.016
0.318CysPro: 0.318 ± 0.021
0.23CysGln: 0.23 ± 0.015
0.249CysArg: 0.249 ± 0.016
0.429CysSer: 0.429 ± 0.024
0.339CysThr: 0.339 ± 0.018
0.372CysVal: 0.372 ± 0.018
0.045CysTrp: 0.045 ± 0.007
0.248CysTyr: 0.248 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.078AspAla: 3.078 ± 0.057
0.344AspCys: 0.344 ± 0.018
2.807AspAsp: 2.807 ± 0.06
5.077AspGlu: 5.077 ± 0.081
2.468AspPhe: 2.468 ± 0.048
3.38AspGly: 3.38 ± 0.055
1.242AspHis: 1.242 ± 0.039
4.546AspIle: 4.546 ± 0.07
3.409AspLys: 3.409 ± 0.065
5.154AspLeu: 5.154 ± 0.071
1.497AspMet: 1.497 ± 0.039
2.112AspAsn: 2.112 ± 0.055
1.925AspPro: 1.925 ± 0.046
2.45AspGln: 2.45 ± 0.046
2.154AspArg: 2.154 ± 0.053
2.806AspSer: 2.806 ± 0.058
2.685AspThr: 2.685 ± 0.049
4.075AspVal: 4.075 ± 0.075
0.639AspTrp: 0.639 ± 0.025
2.426AspTyr: 2.426 ± 0.062
0.0AspXaa: 0.0 ± 0.0
Glu
5.023GluAla: 5.023 ± 0.077
0.341GluCys: 0.341 ± 0.017
4.522GluAsp: 4.522 ± 0.085
7.863GluGlu: 7.863 ± 0.109
2.575GluPhe: 2.575 ± 0.048
4.563GluGly: 4.563 ± 0.083
1.511GluHis: 1.511 ± 0.041
5.674GluIle: 5.674 ± 0.084
6.779GluLys: 6.779 ± 0.098
6.881GluLeu: 6.881 ± 0.091
2.458GluMet: 2.458 ± 0.047
4.341GluAsn: 4.341 ± 0.068
2.078GluPro: 2.078 ± 0.051
3.791GluGln: 3.791 ± 0.072
3.443GluArg: 3.443 ± 0.064
3.779GluSer: 3.779 ± 0.063
3.897GluThr: 3.897 ± 0.06
5.267GluVal: 5.267 ± 0.08
0.924GluTrp: 0.924 ± 0.031
2.4GluTyr: 2.4 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
2.679PheAla: 2.679 ± 0.059
0.296PheCys: 0.296 ± 0.018
2.409PheAsp: 2.409 ± 0.054
2.869PheGlu: 2.869 ± 0.057
2.345PhePhe: 2.345 ± 0.059
3.329PheGly: 3.329 ± 0.071
1.072PheHis: 1.072 ± 0.039
3.937PheIle: 3.937 ± 0.082
2.332PheLys: 2.332 ± 0.055
4.527PheLeu: 4.527 ± 0.085
1.31PheMet: 1.31 ± 0.039
1.922PheAsn: 1.922 ± 0.042
1.65PhePro: 1.65 ± 0.048
1.852PheGln: 1.852 ± 0.042
1.502PheArg: 1.502 ± 0.041
3.199PheSer: 3.199 ± 0.059
2.58PheThr: 2.58 ± 0.054
3.198PheVal: 3.198 ± 0.058
0.471PheTrp: 0.471 ± 0.024
1.855PheTyr: 1.855 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
4.61GlyAla: 4.61 ± 0.092
0.508GlyCys: 0.508 ± 0.025
3.397GlyAsp: 3.397 ± 0.068
4.753GlyGlu: 4.753 ± 0.075
3.402GlyPhe: 3.402 ± 0.063
4.783GlyGly: 4.783 ± 0.097
1.398GlyHis: 1.398 ± 0.035
6.052GlyIle: 6.052 ± 0.084
4.693GlyLys: 4.693 ± 0.078
6.479GlyLeu: 6.479 ± 0.106
2.183GlyMet: 2.183 ± 0.046
2.867GlyAsn: 2.867 ± 0.062
1.792GlyPro: 1.792 ± 0.043
2.289GlyGln: 2.289 ± 0.062
2.514GlyArg: 2.514 ± 0.055
3.892GlySer: 3.892 ± 0.061
3.877GlyThr: 3.877 ± 0.071
4.92GlyVal: 4.92 ± 0.08
0.744GlyTrp: 0.744 ± 0.031
2.862GlyTyr: 2.862 ± 0.054
0.0GlyXaa: 0.0 ± 0.0
His
1.242HisAla: 1.242 ± 0.037
0.164HisCys: 0.164 ± 0.013
1.066HisAsp: 1.066 ± 0.034
1.51HisGlu: 1.51 ± 0.041
1.13HisPhe: 1.13 ± 0.035
1.326HisGly: 1.326 ± 0.035
0.703HisHis: 0.703 ± 0.031
1.602HisIle: 1.602 ± 0.047
1.128HisLys: 1.128 ± 0.038
2.281HisLeu: 2.281 ± 0.048
0.54HisMet: 0.54 ± 0.021
0.906HisAsn: 0.906 ± 0.034
1.097HisPro: 1.097 ± 0.033
1.05HisGln: 1.05 ± 0.034
0.871HisArg: 0.871 ± 0.032
1.25HisSer: 1.25 ± 0.04
1.139HisThr: 1.139 ± 0.032
1.398HisVal: 1.398 ± 0.038
0.218HisTrp: 0.218 ± 0.015
0.934HisTyr: 0.934 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
5.593IleAla: 5.593 ± 0.09
0.571IleCys: 0.571 ± 0.022
4.45IleAsp: 4.45 ± 0.067
5.726IleGlu: 5.726 ± 0.077
3.429IlePhe: 3.429 ± 0.067
6.074IleGly: 6.074 ± 0.101
1.835IleHis: 1.835 ± 0.047
5.921IleIle: 5.921 ± 0.103
4.697IleLys: 4.697 ± 0.082
7.347IleLeu: 7.347 ± 0.104
1.949IleMet: 1.949 ± 0.041
3.372IleAsn: 3.372 ± 0.055
3.553IlePro: 3.553 ± 0.062
3.336IleGln: 3.336 ± 0.059
3.034IleArg: 3.034 ± 0.062
5.146IleSer: 5.146 ± 0.081
4.528IleThr: 4.528 ± 0.073
5.138IleVal: 5.138 ± 0.078
0.721IleTrp: 0.721 ± 0.027
2.581IleTyr: 2.581 ± 0.056
0.0IleXaa: 0.0 ± 0.0
Lys
4.406LysAla: 4.406 ± 0.06
0.274LysCys: 0.274 ± 0.018
4.089LysAsp: 4.089 ± 0.07
7.138LysGlu: 7.138 ± 0.094
1.875LysPhe: 1.875 ± 0.044
4.536LysGly: 4.536 ± 0.061
1.428LysHis: 1.428 ± 0.04
4.466LysIle: 4.466 ± 0.068
5.684LysLys: 5.684 ± 0.096
5.488LysLeu: 5.488 ± 0.08
2.162LysMet: 2.162 ± 0.048
3.551LysAsn: 3.551 ± 0.065
2.285LysPro: 2.285 ± 0.054
3.413LysGln: 3.413 ± 0.071
3.522LysArg: 3.522 ± 0.065
3.573LysSer: 3.573 ± 0.063
3.516LysThr: 3.516 ± 0.066
4.618LysVal: 4.618 ± 0.08
0.922LysTrp: 0.922 ± 0.03
2.156LysTyr: 2.156 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
6.535LeuAla: 6.535 ± 0.1
0.574LeuCys: 0.574 ± 0.024
4.84LeuAsp: 4.84 ± 0.073
6.654LeuGlu: 6.654 ± 0.087
4.922LeuPhe: 4.922 ± 0.095
6.297LeuGly: 6.297 ± 0.096
1.95LeuHis: 1.95 ± 0.046
7.199LeuIle: 7.199 ± 0.115
6.504LeuLys: 6.504 ± 0.087
9.417LeuLeu: 9.417 ± 0.147
2.485LeuMet: 2.485 ± 0.058
4.56LeuAsn: 4.56 ± 0.064
3.824LeuPro: 3.824 ± 0.066
3.625LeuGln: 3.625 ± 0.06
3.444LeuArg: 3.444 ± 0.07
6.648LeuSer: 6.648 ± 0.095
5.479LeuThr: 5.479 ± 0.075
5.82LeuVal: 5.82 ± 0.089
0.812LeuTrp: 0.812 ± 0.034
3.099LeuTyr: 3.099 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
2.001MetAla: 2.001 ± 0.049
0.141MetCys: 0.141 ± 0.012
1.777MetAsp: 1.777 ± 0.043
2.157MetGlu: 2.157 ± 0.051
1.071MetPhe: 1.071 ± 0.033
1.98MetGly: 1.98 ± 0.041
0.414MetHis: 0.414 ± 0.019
2.237MetIle: 2.237 ± 0.051
2.545MetLys: 2.545 ± 0.047
2.448MetLeu: 2.448 ± 0.066
0.902MetMet: 0.902 ± 0.033
1.76MetAsn: 1.76 ± 0.041
0.98MetPro: 0.98 ± 0.038
0.896MetGln: 0.896 ± 0.03
0.984MetArg: 0.984 ± 0.028
1.825MetSer: 1.825 ± 0.042
1.596MetThr: 1.596 ± 0.038
1.968MetVal: 1.968 ± 0.046
0.187MetTrp: 0.187 ± 0.015
0.754MetTyr: 0.754 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
2.566AsnAla: 2.566 ± 0.05
0.28AsnCys: 0.28 ± 0.018
2.457AsnAsp: 2.457 ± 0.054
3.885AsnGlu: 3.885 ± 0.068
1.721AsnPhe: 1.721 ± 0.042
3.301AsnGly: 3.301 ± 0.06
1.388AsnHis: 1.388 ± 0.039
3.554AsnIle: 3.554 ± 0.063
2.975AsnLys: 2.975 ± 0.063
4.013AsnLeu: 4.013 ± 0.07
1.339AsnMet: 1.339 ± 0.036
2.121AsnAsn: 2.121 ± 0.063
2.335AsnPro: 2.335 ± 0.049
2.948AsnGln: 2.948 ± 0.056
2.383AsnArg: 2.383 ± 0.051
2.256AsnSer: 2.256 ± 0.046
2.365AsnThr: 2.365 ± 0.051
3.182AsnVal: 3.182 ± 0.057
0.589AsnTrp: 0.589 ± 0.024
1.603AsnTyr: 1.603 ± 0.047
0.0AsnXaa: 0.0 ± 0.0
Pro
2.081ProAla: 2.081 ± 0.05
0.172ProCys: 0.172 ± 0.011
2.271ProAsp: 2.271 ± 0.052
3.17ProGlu: 3.17 ± 0.063
1.983ProPhe: 1.983 ± 0.047
2.32ProGly: 2.32 ± 0.048
0.8ProHis: 0.8 ± 0.028
2.854ProIle: 2.854 ± 0.048
2.194ProLys: 2.194 ± 0.05
3.362ProLeu: 3.362 ± 0.053
0.823ProMet: 0.823 ± 0.03
1.829ProAsn: 1.829 ± 0.04
0.96ProPro: 0.96 ± 0.035
1.158ProGln: 1.158 ± 0.034
1.069ProArg: 1.069 ± 0.033
2.241ProSer: 2.241 ± 0.053
1.763ProThr: 1.763 ± 0.038
2.828ProVal: 2.828 ± 0.057
0.358ProTrp: 0.358 ± 0.02
1.471ProTyr: 1.471 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.776GlnAla: 2.776 ± 0.057
0.205GlnCys: 0.205 ± 0.017
2.185GlnAsp: 2.185 ± 0.05
3.396GlnGlu: 3.396 ± 0.062
1.716GlnPhe: 1.716 ± 0.041
2.365GlnGly: 2.365 ± 0.046
0.809GlnHis: 0.809 ± 0.028
2.844GlnIle: 2.844 ± 0.057
3.235GlnLys: 3.235 ± 0.06
3.99GlnLeu: 3.99 ± 0.065
1.318GlnMet: 1.318 ± 0.039
2.228GlnAsn: 2.228 ± 0.05
1.262GlnPro: 1.262 ± 0.04
2.052GlnGln: 2.052 ± 0.053
1.522GlnArg: 1.522 ± 0.039
2.685GlnSer: 2.685 ± 0.059
2.261GlnThr: 2.261 ± 0.046
2.52GlnVal: 2.52 ± 0.05
0.449GlnTrp: 0.449 ± 0.025
1.519GlnTyr: 1.519 ± 0.043
0.0GlnXaa: 0.0 ± 0.0
Arg
2.173ArgAla: 2.173 ± 0.057
0.221ArgCys: 0.221 ± 0.015
2.092ArgAsp: 2.092 ± 0.057
3.219ArgGlu: 3.219 ± 0.052
1.902ArgPhe: 1.902 ± 0.042
2.219ArgGly: 2.219 ± 0.053
0.822ArgHis: 0.822 ± 0.026
3.096ArgIle: 3.096 ± 0.055
3.337ArgLys: 3.337 ± 0.069
3.853ArgLeu: 3.853 ± 0.071
1.295ArgMet: 1.295 ± 0.035
2.053ArgAsn: 2.053 ± 0.044
1.236ArgPro: 1.236 ± 0.037
1.504ArgGln: 1.504 ± 0.035
1.79ArgArg: 1.79 ± 0.047
2.187ArgSer: 2.187 ± 0.049
2.012ArgThr: 2.012 ± 0.046
2.538ArgVal: 2.538 ± 0.061
0.364ArgTrp: 0.364 ± 0.021
1.523ArgTyr: 1.523 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
3.43SerAla: 3.43 ± 0.072
0.343SerCys: 0.343 ± 0.02
3.033SerAsp: 3.033 ± 0.052
4.112SerGlu: 4.112 ± 0.063
3.271SerPhe: 3.271 ± 0.068
4.272SerGly: 4.272 ± 0.075
1.26SerHis: 1.26 ± 0.037
5.262SerIle: 5.262 ± 0.071
3.84SerLys: 3.84 ± 0.071
5.967SerLeu: 5.967 ± 0.093
1.773SerMet: 1.773 ± 0.047
2.702SerAsn: 2.702 ± 0.054
2.165SerPro: 2.165 ± 0.054
2.203SerGln: 2.203 ± 0.048
2.263SerArg: 2.263 ± 0.045
3.948SerSer: 3.948 ± 0.072
3.059SerThr: 3.059 ± 0.061
4.094SerVal: 4.094 ± 0.065
0.584SerTrp: 0.584 ± 0.027
2.277SerTyr: 2.277 ± 0.055
0.0SerXaa: 0.0 ± 0.0
Thr
3.563ThrAla: 3.563 ± 0.062
0.345ThrCys: 0.345 ± 0.018
2.906ThrAsp: 2.906 ± 0.053
3.586ThrGlu: 3.586 ± 0.067
2.723ThrPhe: 2.723 ± 0.056
4.092ThrGly: 4.092 ± 0.07
1.032ThrHis: 1.032 ± 0.032
4.556ThrIle: 4.556 ± 0.066
3.284ThrLys: 3.284 ± 0.059
5.123ThrLeu: 5.123 ± 0.073
1.333ThrMet: 1.333 ± 0.041
2.573ThrAsn: 2.573 ± 0.049
2.194ThrPro: 2.194 ± 0.046
1.539ThrGln: 1.539 ± 0.035
1.815ThrArg: 1.815 ± 0.042
3.361ThrSer: 3.361 ± 0.058
2.84ThrThr: 2.84 ± 0.05
3.816ThrVal: 3.816 ± 0.063
0.555ThrTrp: 0.555 ± 0.025
1.969ThrTyr: 1.969 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
4.306ValAla: 4.306 ± 0.073
0.569ValCys: 0.569 ± 0.022
3.895ValAsp: 3.895 ± 0.063
5.041ValGlu: 5.041 ± 0.084
2.98ValPhe: 2.98 ± 0.053
4.348ValGly: 4.348 ± 0.085
1.353ValHis: 1.353 ± 0.039
5.642ValIle: 5.642 ± 0.088
4.729ValLys: 4.729 ± 0.074
6.676ValLeu: 6.676 ± 0.089
1.959ValMet: 1.959 ± 0.049
3.268ValAsn: 3.268 ± 0.054
2.547ValPro: 2.547 ± 0.049
2.587ValGln: 2.587 ± 0.052
2.597ValArg: 2.597 ± 0.052
4.335ValSer: 4.335 ± 0.071
3.825ValThr: 3.825 ± 0.063
4.886ValVal: 4.886 ± 0.077
0.617ValTrp: 0.617 ± 0.029
2.279ValTyr: 2.279 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
0.575TrpAla: 0.575 ± 0.029
0.061TrpCys: 0.061 ± 0.008
0.541TrpAsp: 0.541 ± 0.025
0.705TrpGlu: 0.705 ± 0.027
0.573TrpPhe: 0.573 ± 0.028
0.695TrpGly: 0.695 ± 0.029
0.192TrpHis: 0.192 ± 0.015
0.944TrpIle: 0.944 ± 0.035
0.739TrpLys: 0.739 ± 0.028
1.103TrpLeu: 1.103 ± 0.037
0.348TrpMet: 0.348 ± 0.018
0.572TrpAsn: 0.572 ± 0.025
0.292TrpPro: 0.292 ± 0.017
0.362TrpGln: 0.362 ± 0.019
0.366TrpArg: 0.366 ± 0.019
0.552TrpSer: 0.552 ± 0.026
0.503TrpThr: 0.503 ± 0.021
0.671TrpVal: 0.671 ± 0.028
0.147TrpTrp: 0.147 ± 0.012
0.358TrpTyr: 0.358 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.004TyrAla: 2.004 ± 0.042
0.234TyrCys: 0.234 ± 0.014
2.038TyrAsp: 2.038 ± 0.052
2.68TyrGlu: 2.68 ± 0.055
1.864TyrPhe: 1.864 ± 0.05
2.529TyrGly: 2.529 ± 0.055
0.938TyrHis: 0.938 ± 0.03
2.614TyrIle: 2.614 ± 0.051
2.118TyrLys: 2.118 ± 0.051
3.558TyrLeu: 3.558 ± 0.06
0.909TyrMet: 0.909 ± 0.031
1.502TyrAsn: 1.502 ± 0.046
1.441TyrPro: 1.441 ± 0.038
1.894TyrGln: 1.894 ± 0.049
1.595TyrArg: 1.595 ± 0.034
2.013TyrSer: 2.013 ± 0.044
1.824TyrThr: 1.824 ± 0.048
2.315TyrVal: 2.315 ± 0.044
0.407TyrTrp: 0.407 ± 0.02
1.466TyrTyr: 1.466 ± 0.04
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3528 proteins (977637 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski