Amino acid dipepetide frequency for Pseudomonas phage phi2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.101AlaAla: 19.101 ± 2.065
0.914AlaCys: 0.914 ± 0.315
7.22AlaAsp: 7.22 ± 0.651
8.774AlaGlu: 8.774 ± 1.285
3.564AlaPhe: 3.564 ± 0.622
9.413AlaGly: 9.413 ± 1.069
1.554AlaHis: 1.554 ± 0.317
5.758AlaIle: 5.758 ± 0.642
5.301AlaLys: 5.301 ± 0.936
13.069AlaLeu: 13.069 ± 1.515
3.29AlaMet: 3.29 ± 0.62
2.193AlaAsn: 2.193 ± 0.397
5.209AlaPro: 5.209 ± 0.663
4.935AlaGln: 4.935 ± 0.742
9.048AlaArg: 9.048 ± 1.026
7.768AlaSer: 7.768 ± 1.856
5.666AlaThr: 5.666 ± 0.594
6.763AlaVal: 6.763 ± 0.666
1.919AlaTrp: 1.919 ± 0.378
2.65AlaTyr: 2.65 ± 0.489
0.0AlaXaa: 0.0 ± 0.0
Cys
1.279CysAla: 1.279 ± 0.356
0.274CysCys: 0.274 ± 0.15
0.64CysAsp: 0.64 ± 0.222
1.005CysGlu: 1.005 ± 0.399
0.091CysPhe: 0.091 ± 0.098
1.188CysGly: 1.188 ± 0.321
0.457CysHis: 0.457 ± 0.2
0.366CysIle: 0.366 ± 0.166
0.274CysLys: 0.274 ± 0.174
0.457CysLeu: 0.457 ± 0.209
0.0CysMet: 0.0 ± 0.0
0.64CysAsn: 0.64 ± 0.289
0.548CysPro: 0.548 ± 0.262
0.0CysGln: 0.0 ± 0.0
1.371CysArg: 1.371 ± 0.392
0.731CysSer: 0.731 ± 0.24
0.274CysThr: 0.274 ± 0.158
0.548CysVal: 0.548 ± 0.224
0.548CysTrp: 0.548 ± 0.241
0.183CysTyr: 0.183 ± 0.197
0.0CysXaa: 0.0 ± 0.0
Asp
6.306AspAla: 6.306 ± 0.739
0.457AspCys: 0.457 ± 0.258
4.295AspAsp: 4.295 ± 0.753
3.838AspGlu: 3.838 ± 0.604
1.645AspPhe: 1.645 ± 0.424
5.483AspGly: 5.483 ± 0.591
0.823AspHis: 0.823 ± 0.254
2.193AspIle: 2.193 ± 0.504
1.188AspLys: 1.188 ± 0.453
6.123AspLeu: 6.123 ± 0.842
0.731AspMet: 0.731 ± 0.256
0.823AspAsn: 0.823 ± 0.285
3.564AspPro: 3.564 ± 0.688
2.742AspGln: 2.742 ± 0.5
2.742AspArg: 2.742 ± 0.426
2.102AspSer: 2.102 ± 0.402
2.011AspThr: 2.011 ± 0.43
3.381AspVal: 3.381 ± 0.523
1.554AspTrp: 1.554 ± 0.374
1.919AspTyr: 1.919 ± 0.58
0.0AspXaa: 0.0 ± 0.0
Glu
8.317GluAla: 8.317 ± 0.779
1.097GluCys: 1.097 ± 0.345
3.199GluAsp: 3.199 ± 0.454
4.57GluGlu: 4.57 ± 0.593
2.193GluPhe: 2.193 ± 0.381
4.752GluGly: 4.752 ± 0.618
0.64GluHis: 0.64 ± 0.312
3.93GluIle: 3.93 ± 0.577
3.473GluLys: 3.473 ± 0.482
5.849GluLeu: 5.849 ± 0.881
2.285GluMet: 2.285 ± 0.328
2.285GluAsn: 2.285 ± 0.419
3.747GluPro: 3.747 ± 0.647
3.564GluGln: 3.564 ± 0.51
5.94GluArg: 5.94 ± 0.848
3.564GluSer: 3.564 ± 0.652
2.559GluThr: 2.559 ± 0.463
4.661GluVal: 4.661 ± 0.6
1.097GluTrp: 1.097 ± 0.281
1.005GluTyr: 1.005 ± 0.302
0.0GluXaa: 0.0 ± 0.0
Phe
2.559PheAla: 2.559 ± 0.495
0.366PheCys: 0.366 ± 0.164
1.462PheAsp: 1.462 ± 0.293
2.376PheGlu: 2.376 ± 0.533
1.005PhePhe: 1.005 ± 0.312
3.656PheGly: 3.656 ± 0.647
0.64PheHis: 0.64 ± 0.235
0.914PheIle: 0.914 ± 0.387
1.736PheLys: 1.736 ± 0.308
1.554PheLeu: 1.554 ± 0.363
0.731PheMet: 0.731 ± 0.289
1.645PheAsn: 1.645 ± 0.397
1.279PhePro: 1.279 ± 0.346
0.274PheGln: 0.274 ± 0.188
1.645PheArg: 1.645 ± 0.338
1.736PheSer: 1.736 ± 0.401
1.645PheThr: 1.645 ± 0.399
2.285PheVal: 2.285 ± 0.465
0.731PheTrp: 0.731 ± 0.246
1.005PheTyr: 1.005 ± 0.333
0.0PheXaa: 0.0 ± 0.0
Gly
7.494GlyAla: 7.494 ± 0.78
0.731GlyCys: 0.731 ± 0.291
4.57GlyAsp: 4.57 ± 0.662
4.935GlyGlu: 4.935 ± 0.589
2.742GlyPhe: 2.742 ± 0.457
6.763GlyGly: 6.763 ± 1.106
1.554GlyHis: 1.554 ± 0.35
3.016GlyIle: 3.016 ± 0.661
3.564GlyLys: 3.564 ± 0.479
6.946GlyLeu: 6.946 ± 0.817
2.559GlyMet: 2.559 ± 0.483
2.925GlyAsn: 2.925 ± 0.689
2.468GlyPro: 2.468 ± 0.362
3.473GlyGln: 3.473 ± 0.705
5.392GlyArg: 5.392 ± 0.729
3.838GlySer: 3.838 ± 0.56
4.935GlyThr: 4.935 ± 0.657
5.392GlyVal: 5.392 ± 0.586
1.919GlyTrp: 1.919 ± 0.358
2.468GlyTyr: 2.468 ± 0.614
0.0GlyXaa: 0.0 ± 0.0
His
1.554HisAla: 1.554 ± 0.412
0.091HisCys: 0.091 ± 0.08
0.914HisAsp: 0.914 ± 0.246
1.462HisGlu: 1.462 ± 0.329
0.366HisPhe: 0.366 ± 0.175
0.823HisGly: 0.823 ± 0.355
0.183HisHis: 0.183 ± 0.149
0.823HisIle: 0.823 ± 0.298
0.823HisLys: 0.823 ± 0.313
1.188HisLeu: 1.188 ± 0.314
0.457HisMet: 0.457 ± 0.198
0.457HisAsn: 0.457 ± 0.239
1.279HisPro: 1.279 ± 0.31
0.823HisGln: 0.823 ± 0.285
1.005HisArg: 1.005 ± 0.321
0.64HisSer: 0.64 ± 0.219
1.097HisThr: 1.097 ± 0.343
1.462HisVal: 1.462 ± 0.369
0.366HisTrp: 0.366 ± 0.17
0.457HisTyr: 0.457 ± 0.174
0.0HisXaa: 0.0 ± 0.0
Ile
5.027IleAla: 5.027 ± 0.695
0.64IleCys: 0.64 ± 0.248
2.833IleAsp: 2.833 ± 0.407
4.113IleGlu: 4.113 ± 0.53
1.005IlePhe: 1.005 ± 0.333
2.833IleGly: 2.833 ± 0.538
0.731IleHis: 0.731 ± 0.229
1.097IleIle: 1.097 ± 0.355
1.828IleLys: 1.828 ± 0.404
2.65IleLeu: 2.65 ± 0.421
0.914IleMet: 0.914 ± 0.257
1.736IleAsn: 1.736 ± 0.462
2.559IlePro: 2.559 ± 0.47
2.011IleGln: 2.011 ± 0.431
3.473IleArg: 3.473 ± 0.491
3.199IleSer: 3.199 ± 0.458
2.833IleThr: 2.833 ± 0.563
2.742IleVal: 2.742 ± 0.42
0.366IleTrp: 0.366 ± 0.23
2.011IleTyr: 2.011 ± 0.404
0.0IleXaa: 0.0 ± 0.0
Lys
5.666LysAla: 5.666 ± 0.889
0.731LysCys: 0.731 ± 0.226
1.462LysAsp: 1.462 ± 0.369
2.285LysGlu: 2.285 ± 0.54
0.823LysPhe: 0.823 ± 0.288
1.645LysGly: 1.645 ± 0.347
0.823LysHis: 0.823 ± 0.298
2.193LysIle: 2.193 ± 0.463
2.285LysLys: 2.285 ± 0.548
3.473LysLeu: 3.473 ± 0.425
1.005LysMet: 1.005 ± 0.29
0.823LysAsn: 0.823 ± 0.29
1.462LysPro: 1.462 ± 0.303
2.468LysGln: 2.468 ± 0.53
3.381LysArg: 3.381 ± 0.629
2.193LysSer: 2.193 ± 0.652
1.554LysThr: 1.554 ± 0.371
2.559LysVal: 2.559 ± 0.564
0.823LysTrp: 0.823 ± 0.27
0.64LysTyr: 0.64 ± 0.254
0.0LysXaa: 0.0 ± 0.0
Leu
10.967LeuAla: 10.967 ± 1.449
0.548LeuCys: 0.548 ± 0.285
4.661LeuAsp: 4.661 ± 0.43
5.666LeuGlu: 5.666 ± 0.753
2.742LeuPhe: 2.742 ± 0.379
5.118LeuGly: 5.118 ± 0.649
1.462LeuHis: 1.462 ± 0.442
3.381LeuIle: 3.381 ± 0.442
4.295LeuLys: 4.295 ± 0.686
6.397LeuLeu: 6.397 ± 0.938
2.102LeuMet: 2.102 ± 0.468
3.381LeuAsn: 3.381 ± 0.56
4.844LeuPro: 4.844 ± 0.637
3.838LeuGln: 3.838 ± 0.858
7.677LeuArg: 7.677 ± 1.06
5.301LeuSer: 5.301 ± 0.632
4.752LeuThr: 4.752 ± 0.585
5.94LeuVal: 5.94 ± 0.56
0.457LeuTrp: 0.457 ± 0.153
2.376LeuTyr: 2.376 ± 0.477
0.0LeuXaa: 0.0 ± 0.0
Met
2.742MetAla: 2.742 ± 0.462
0.183MetCys: 0.183 ± 0.127
1.188MetAsp: 1.188 ± 0.271
1.279MetGlu: 1.279 ± 0.355
0.914MetPhe: 0.914 ± 0.25
1.462MetGly: 1.462 ± 0.358
1.097MetHis: 1.097 ± 0.349
1.554MetIle: 1.554 ± 0.327
0.731MetLys: 0.731 ± 0.323
2.285MetLeu: 2.285 ± 0.404
0.457MetMet: 0.457 ± 0.199
1.097MetAsn: 1.097 ± 0.343
1.462MetPro: 1.462 ± 0.287
1.645MetGln: 1.645 ± 0.336
1.919MetArg: 1.919 ± 0.499
1.645MetSer: 1.645 ± 0.393
1.097MetThr: 1.097 ± 0.304
1.462MetVal: 1.462 ± 0.422
0.091MetTrp: 0.091 ± 0.09
0.183MetTyr: 0.183 ± 0.142
0.0MetXaa: 0.0 ± 0.0
Asn
4.021AsnAla: 4.021 ± 0.555
0.366AsnCys: 0.366 ± 0.154
1.645AsnAsp: 1.645 ± 0.359
1.645AsnGlu: 1.645 ± 0.39
0.457AsnPhe: 0.457 ± 0.185
3.107AsnGly: 3.107 ± 0.591
0.64AsnHis: 0.64 ± 0.218
0.914AsnIle: 0.914 ± 0.31
0.548AsnLys: 0.548 ± 0.248
2.102AsnLeu: 2.102 ± 0.397
0.64AsnMet: 0.64 ± 0.198
0.823AsnAsn: 0.823 ± 0.329
2.011AsnPro: 2.011 ± 0.447
0.548AsnGln: 0.548 ± 0.258
3.016AsnArg: 3.016 ± 0.672
2.011AsnSer: 2.011 ± 0.333
3.473AsnThr: 3.473 ± 1.279
2.102AsnVal: 2.102 ± 0.446
1.005AsnTrp: 1.005 ± 0.363
0.548AsnTyr: 0.548 ± 0.226
0.0AsnXaa: 0.0 ± 0.0
Pro
6.397ProAla: 6.397 ± 1.028
0.731ProCys: 0.731 ± 0.233
2.468ProAsp: 2.468 ± 0.401
5.301ProGlu: 5.301 ± 0.55
0.914ProPhe: 0.914 ± 0.241
4.295ProGly: 4.295 ± 0.609
0.457ProHis: 0.457 ± 0.22
2.833ProIle: 2.833 ± 0.484
0.823ProLys: 0.823 ± 0.199
4.295ProLeu: 4.295 ± 0.566
1.005ProMet: 1.005 ± 0.216
1.462ProAsn: 1.462 ± 0.34
3.199ProPro: 3.199 ± 0.602
1.736ProGln: 1.736 ± 0.413
3.016ProArg: 3.016 ± 0.393
4.113ProSer: 4.113 ± 0.608
3.199ProThr: 3.199 ± 0.549
3.564ProVal: 3.564 ± 0.634
0.914ProTrp: 0.914 ± 0.249
1.005ProTyr: 1.005 ± 0.217
0.0ProXaa: 0.0 ± 0.0
Gln
7.22GlnAla: 7.22 ± 1.182
0.64GlnCys: 0.64 ± 0.271
1.279GlnAsp: 1.279 ± 0.31
2.742GlnGlu: 2.742 ± 0.407
1.828GlnPhe: 1.828 ± 0.39
3.381GlnGly: 3.381 ± 0.607
1.005GlnHis: 1.005 ± 0.295
2.193GlnIle: 2.193 ± 0.426
1.005GlnLys: 1.005 ± 0.229
4.661GlnLeu: 4.661 ± 0.734
1.097GlnMet: 1.097 ± 0.308
0.731GlnAsn: 0.731 ± 0.366
2.559GlnPro: 2.559 ± 0.612
2.468GlnGln: 2.468 ± 0.581
2.559GlnArg: 2.559 ± 0.622
1.828GlnSer: 1.828 ± 0.445
2.011GlnThr: 2.011 ± 0.402
2.925GlnVal: 2.925 ± 0.632
0.274GlnTrp: 0.274 ± 0.134
0.823GlnTyr: 0.823 ± 0.25
0.0GlnXaa: 0.0 ± 0.0
Arg
8.499ArgAla: 8.499 ± 1.241
0.64ArgCys: 0.64 ± 0.239
3.747ArgAsp: 3.747 ± 0.682
4.844ArgGlu: 4.844 ± 0.719
2.65ArgPhe: 2.65 ± 0.618
4.478ArgGly: 4.478 ± 0.649
1.005ArgHis: 1.005 ± 0.327
4.021ArgIle: 4.021 ± 0.612
3.199ArgLys: 3.199 ± 0.612
9.413ArgLeu: 9.413 ± 0.995
1.919ArgMet: 1.919 ± 0.371
1.828ArgAsn: 1.828 ± 0.354
3.199ArgPro: 3.199 ± 0.407
3.747ArgGln: 3.747 ± 0.652
5.575ArgArg: 5.575 ± 1.058
4.752ArgSer: 4.752 ± 0.839
2.833ArgThr: 2.833 ± 0.499
4.204ArgVal: 4.204 ± 0.662
1.828ArgTrp: 1.828 ± 0.392
1.462ArgTyr: 1.462 ± 0.491
0.0ArgXaa: 0.0 ± 0.0
Ser
8.499SerAla: 8.499 ± 1.577
0.823SerCys: 0.823 ± 0.219
2.925SerAsp: 2.925 ± 0.543
3.107SerGlu: 3.107 ± 0.541
2.102SerPhe: 2.102 ± 0.522
5.758SerGly: 5.758 ± 0.729
0.731SerHis: 0.731 ± 0.288
2.376SerIle: 2.376 ± 0.346
2.285SerLys: 2.285 ± 0.438
3.199SerLeu: 3.199 ± 0.407
1.554SerMet: 1.554 ± 0.4
2.833SerAsn: 2.833 ± 1.289
3.107SerPro: 3.107 ± 0.474
2.559SerGln: 2.559 ± 0.511
3.381SerArg: 3.381 ± 0.488
3.747SerSer: 3.747 ± 0.452
3.838SerThr: 3.838 ± 0.607
5.027SerVal: 5.027 ± 0.817
0.548SerTrp: 0.548 ± 0.21
1.279SerTyr: 1.279 ± 0.272
0.0SerXaa: 0.0 ± 0.0
Thr
6.854ThrAla: 6.854 ± 0.758
0.457ThrCys: 0.457 ± 0.188
2.468ThrAsp: 2.468 ± 0.41
2.468ThrGlu: 2.468 ± 0.474
1.554ThrPhe: 1.554 ± 0.312
7.037ThrGly: 7.037 ± 1.34
0.914ThrHis: 0.914 ± 0.292
2.559ThrIle: 2.559 ± 0.389
1.554ThrLys: 1.554 ± 0.414
4.661ThrLeu: 4.661 ± 0.665
1.462ThrMet: 1.462 ± 0.36
1.828ThrAsn: 1.828 ± 0.326
2.833ThrPro: 2.833 ± 0.531
1.279ThrGln: 1.279 ± 0.283
2.742ThrArg: 2.742 ± 0.675
3.29ThrSer: 3.29 ± 0.582
4.295ThrThr: 4.295 ± 0.732
5.118ThrVal: 5.118 ± 0.728
1.462ThrTrp: 1.462 ± 0.399
1.554ThrTyr: 1.554 ± 0.306
0.0ThrXaa: 0.0 ± 0.0
Val
6.397ValAla: 6.397 ± 0.736
0.457ValCys: 0.457 ± 0.236
4.844ValAsp: 4.844 ± 0.623
6.397ValGlu: 6.397 ± 0.6
1.736ValPhe: 1.736 ± 0.367
4.478ValGly: 4.478 ± 0.607
1.005ValHis: 1.005 ± 0.315
2.65ValIle: 2.65 ± 0.48
2.559ValLys: 2.559 ± 0.417
4.387ValLeu: 4.387 ± 0.733
1.279ValMet: 1.279 ± 0.3
2.193ValAsn: 2.193 ± 0.449
4.021ValPro: 4.021 ± 0.581
2.925ValGln: 2.925 ± 0.417
5.027ValArg: 5.027 ± 0.657
4.752ValSer: 4.752 ± 0.807
5.483ValThr: 5.483 ± 0.99
4.57ValVal: 4.57 ± 0.669
0.823ValTrp: 0.823 ± 0.242
1.554ValTyr: 1.554 ± 0.344
0.0ValXaa: 0.0 ± 0.0
Trp
1.736TrpAla: 1.736 ± 0.464
0.274TrpCys: 0.274 ± 0.163
1.097TrpAsp: 1.097 ± 0.28
0.731TrpGlu: 0.731 ± 0.276
0.457TrpPhe: 0.457 ± 0.257
0.64TrpGly: 0.64 ± 0.284
0.274TrpHis: 0.274 ± 0.135
1.188TrpIle: 1.188 ± 0.301
0.183TrpLys: 0.183 ± 0.136
1.554TrpLeu: 1.554 ± 0.339
0.548TrpMet: 0.548 ± 0.205
0.914TrpAsn: 0.914 ± 0.34
0.914TrpPro: 0.914 ± 0.317
0.548TrpGln: 0.548 ± 0.219
1.188TrpArg: 1.188 ± 0.318
0.914TrpSer: 0.914 ± 0.265
1.462TrpThr: 1.462 ± 0.241
2.011TrpVal: 2.011 ± 0.458
0.548TrpTrp: 0.548 ± 0.259
0.366TrpTyr: 0.366 ± 0.139
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.473TyrAla: 3.473 ± 0.59
0.457TyrCys: 0.457 ± 0.193
1.005TyrAsp: 1.005 ± 0.288
1.188TyrGlu: 1.188 ± 0.35
0.457TyrPhe: 0.457 ± 0.195
1.371TyrGly: 1.371 ± 0.332
0.274TyrHis: 0.274 ± 0.159
0.64TyrIle: 0.64 ± 0.232
0.64TyrLys: 0.64 ± 0.248
1.645TyrLeu: 1.645 ± 0.44
0.366TyrMet: 0.366 ± 0.204
1.005TyrAsn: 1.005 ± 0.239
1.462TyrPro: 1.462 ± 0.466
1.736TyrGln: 1.736 ± 0.58
3.747TyrArg: 3.747 ± 0.658
1.554TyrSer: 1.554 ± 0.423
1.371TyrThr: 1.371 ± 0.382
0.823TyrVal: 0.823 ± 0.279
0.274TyrTrp: 0.274 ± 0.164
0.823TyrTyr: 0.823 ± 0.32
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (10943 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski