McMurdo Ice Shelf pond-associated circular DNA virus-2

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075LZ73|A0A075LZ73_9VIRU Uncharacterized protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-2 OX=1521386 PE=4 SV=1
MM1 pKa = 7.15VNARR5 pKa = 11.84CVCVTIHH12 pKa = 6.43VDD14 pKa = 3.58NIFWEE19 pKa = 4.34LQKK22 pKa = 10.47WNQSLTYY29 pKa = 10.54GIGQLEE35 pKa = 4.4LGLNGSTHH43 pKa = 3.99WQMYY47 pKa = 9.07FEE49 pKa = 5.24NNTAISLTQWKK60 pKa = 9.17QLLGCKK66 pKa = 9.29RR67 pKa = 11.84AHH69 pKa = 5.68VEE71 pKa = 4.13TRR73 pKa = 11.84KK74 pKa = 8.84GTALLAIEE82 pKa = 4.28YY83 pKa = 9.15CKK85 pKa = 10.71KK86 pKa = 10.65EE87 pKa = 3.99EE88 pKa = 4.19TRR90 pKa = 11.84LHH92 pKa = 6.14GPGTSFEE99 pKa = 4.27FGKK102 pKa = 7.69TTEE105 pKa = 4.11EE106 pKa = 4.04SVKK109 pKa = 10.43RR110 pKa = 11.84KK111 pKa = 10.51SPDD114 pKa = 3.14SPYY117 pKa = 11.16KK118 pKa = 10.33KK119 pKa = 10.42ALNCLSYY126 pKa = 10.95ADD128 pKa = 4.74SIAIIKK134 pKa = 10.46DD135 pKa = 3.32EE136 pKa = 4.17APRR139 pKa = 11.84DD140 pKa = 3.4YY141 pKa = 10.94VIYY144 pKa = 10.02HH145 pKa = 5.7NQVTSTLKK153 pKa = 10.9AIFKK157 pKa = 8.87PVWNKK162 pKa = 9.28TPGLVFNLEE171 pKa = 4.18PFPEE175 pKa = 4.25EE176 pKa = 3.98TLNTRR181 pKa = 11.84AVIFMGRR188 pKa = 11.84SGLGKK193 pKa = 7.31TQFAISHH200 pKa = 6.41FKK202 pKa = 10.85DD203 pKa = 3.39PLIVSHH209 pKa = 7.66IDD211 pKa = 3.39DD212 pKa = 4.6LKK214 pKa = 11.21KK215 pKa = 10.88LNPTIDD221 pKa = 4.37GIIFDD226 pKa = 5.22DD227 pKa = 4.39MNFQHH232 pKa = 7.06WPPTACIHH240 pKa = 5.92LCDD243 pKa = 3.88MEE245 pKa = 4.71LPRR248 pKa = 11.84SINVKK253 pKa = 9.84YY254 pKa = 9.03GTVEE258 pKa = 3.79IPANTPRR265 pKa = 11.84IFTSNRR271 pKa = 11.84DD272 pKa = 3.59FEE274 pKa = 5.28EE275 pKa = 4.15IWSKK279 pKa = 11.59DD280 pKa = 3.32CTFEE284 pKa = 3.75EE285 pKa = 4.43MNAIRR290 pKa = 11.84RR291 pKa = 11.84RR292 pKa = 11.84CDD294 pKa = 2.33ITTFDD299 pKa = 3.72NEE301 pKa = 4.33LYY303 pKa = 10.88

Molecular weight: 34.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075M3P4|A0A075M3P4_9VIRU Putative capsid protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-2 OX=1521386 PE=4 SV=1
MM1 pKa = 6.79NRR3 pKa = 11.84KK4 pKa = 8.1MKK6 pKa = 9.62RR7 pKa = 11.84TGRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84TYY14 pKa = 9.05RR15 pKa = 11.84RR16 pKa = 11.84KK17 pKa = 9.3RR18 pKa = 11.84NRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84SGRR25 pKa = 11.84SGRR28 pKa = 11.84TRR30 pKa = 11.84RR31 pKa = 11.84LKK33 pKa = 10.47FRR35 pKa = 11.84NTNLQSKK42 pKa = 9.63LVVFRR47 pKa = 11.84DD48 pKa = 3.41KK49 pKa = 11.11FRR51 pKa = 11.84VEE53 pKa = 4.0VQGNPNSVDD62 pKa = 3.15GVSIANSGNFNGISSAEE79 pKa = 3.94YY80 pKa = 8.81SRR82 pKa = 11.84GVRR85 pKa = 11.84EE86 pKa = 3.59CRR88 pKa = 11.84GFNRR92 pKa = 11.84IAFSGFSVSVKK103 pKa = 9.13KK104 pKa = 8.66TAEE107 pKa = 4.16CEE109 pKa = 3.93TQYY112 pKa = 10.81TVNPTVAVSAIAVNKK127 pKa = 8.38TRR129 pKa = 11.84STGRR133 pKa = 11.84LSFVTDD139 pKa = 4.18LYY141 pKa = 11.71VDD143 pKa = 4.36TTQMAANDD151 pKa = 3.86VFTEE155 pKa = 4.39TVLGVKK161 pKa = 10.25KK162 pKa = 10.75NIGFKK167 pKa = 9.99GRR169 pKa = 11.84KK170 pKa = 7.48VYY172 pKa = 9.22TYY174 pKa = 10.41KK175 pKa = 10.58VSKK178 pKa = 9.87HH179 pKa = 4.81LHH181 pKa = 5.81AGQSIKK187 pKa = 11.07VKK189 pKa = 10.67DD190 pKa = 3.97AFLTSTPPNIPWPLLLNFQSDD211 pKa = 3.86TTLTKK216 pKa = 9.39QTVPGGFFLVCDD228 pKa = 4.33TWPTAPLAPATIINTFPWHH247 pKa = 4.96TTSCQVHH254 pKa = 5.54INYY257 pKa = 9.16YY258 pKa = 8.97FKK260 pKa = 11.01VWGKK264 pKa = 8.75QATMLPLAWGG274 pKa = 3.62

Molecular weight: 30.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

863

98

303

215.8

24.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.867 ± 0.375

2.433 ± 0.36

4.751 ± 0.636

5.446 ± 1.302

5.098 ± 0.378

6.489 ± 0.855

2.781 ± 0.489

5.91 ± 1.099

5.794 ± 0.861

6.837 ± 0.987

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.506 ± 0.203

5.794 ± 0.324

5.678 ± 1.063

3.824 ± 0.567

8.691 ± 1.843

5.91 ± 0.877

7.532 ± 1.317

5.91 ± 1.157

1.97 ± 0.158

2.781 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski