Bifidobacterium primatium
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2029 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2M9HBD6|A0A2M9HBD6_9BIFI Amino acid ABC transporter substrate-binding protein OS=Bifidobacterium primatium OX=2045438 GN=CS006_03055 PE=3 SV=1
MM1 pKa = 7.52 IGGIVALAIIIAVVLAVIGVVGGHH25 pKa = 6.25 SDD27 pKa = 3.48 DD28 pKa = 3.37 STYY31 pKa = 11.63 ADD33 pKa = 3.7 GQQGYY38 pKa = 10.33 DD39 pKa = 3.53 SVPFDD44 pKa = 5.49 DD45 pKa = 5.66 PYY47 pKa = 11.73 QMQRR51 pKa = 11.84 DD52 pKa = 3.72 ISSITGSSCLWSDD65 pKa = 2.92 IGSYY69 pKa = 10.19 SALGGDD75 pKa = 4.62 SEE77 pKa = 4.72 ASSIPGLDD85 pKa = 3.29 GAYY88 pKa = 8.94 TCGDD92 pKa = 3.88 DD93 pKa = 5.44 EE94 pKa = 4.79 ILLMFDD100 pKa = 3.34 TTSDD104 pKa = 3.15 TRR106 pKa = 11.84 QMVTTLEE113 pKa = 4.0 RR114 pKa = 11.84 SLAQGDD120 pKa = 4.0 DD121 pKa = 3.82 TFDD124 pKa = 3.47 TTVTDD129 pKa = 3.38 SLGDD133 pKa = 3.6 YY134 pKa = 10.88 AVVHH138 pKa = 7.44 DD139 pKa = 4.83 DD140 pKa = 2.69 TWMVIGKK147 pKa = 8.77 NADD150 pKa = 3.07 MKK152 pKa = 10.64 QLHH155 pKa = 6.56 DD156 pKa = 3.46 AWGGDD161 pKa = 3.49 MQRR164 pKa = 11.84 LDD166 pKa = 4.0 EE167 pKa = 4.76 LADD170 pKa = 3.58 SGSRR174 pKa = 11.84 DD175 pKa = 3.72 TII177 pKa = 3.73
Molecular weight: 18.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.567
IPC_protein 3.617
Toseland 3.363
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.63
Rodwell 3.439
Grimsley 3.274
Solomon 3.63
Lehninger 3.579
Nozaki 3.757
DTASelect 4.088
Thurlkill 3.439
EMBOSS 3.63
Sillero 3.745
Patrickios 1.863
IPC_peptide 3.617
IPC2_peptide 3.706
IPC2.peptide.svr19 3.712
Protein with the highest isoelectric point:
>tr|A0A2M9HBS7|A0A2M9HBS7_9BIFI Non-specific serine/threonine protein kinase OS=Bifidobacterium primatium OX=2045438 GN=CS006_03860 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 4.79 MKK15 pKa = 9.36 HH16 pKa = 5.81 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 SGRR28 pKa = 11.84 ALINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.79 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2029
0
2029
759016
24
3146
374.1
40.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.098 ± 0.082
0.876 ± 0.017
6.793 ± 0.053
5.581 ± 0.055
3.468 ± 0.037
8.322 ± 0.046
2.127 ± 0.026
5.314 ± 0.041
3.994 ± 0.053
8.631 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.573 ± 0.025
3.294 ± 0.038
4.595 ± 0.036
3.05 ± 0.03
6.155 ± 0.069
6.034 ± 0.056
6.091 ± 0.049
7.914 ± 0.044
1.309 ± 0.022
2.781 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here