Halobacterium virus ChaoS9
Average proteome isoelectric point is 4.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A481V794|A0A481V794_9CAUD VapC family toxin OS=Halobacterium virus ChaoS9 OX=2496992 GN=ChaoS9_235 PE=4 SV=1
MM1 pKa = 6.99 IQAIFKK7 pKa = 10.3 PDD9 pKa = 3.57 DD10 pKa = 3.48 GTNRR14 pKa = 11.84 MVGHH18 pKa = 6.74 VSTEE22 pKa = 3.83 YY23 pKa = 8.51 TTDD26 pKa = 3.65 DD27 pKa = 3.4 EE28 pKa = 5.5 SEE30 pKa = 4.18 ILVEE34 pKa = 3.94 TSIGEE39 pKa = 3.89 LSEE42 pKa = 4.46 AFEE45 pKa = 4.46 DD46 pKa = 4.56 APVEE50 pKa = 3.92 EE51 pKa = 5.37 LPDD54 pKa = 3.69 IGHH57 pKa = 6.98 PVNLAIEE64 pKa = 4.4 NPLTYY69 pKa = 9.26 RR70 pKa = 11.84 DD71 pKa = 3.97 YY72 pKa = 11.62 LILEE76 pKa = 4.65 NGDD79 pKa = 3.35 VVYY82 pKa = 10.75 DD83 pKa = 3.91 EE84 pKa = 5.03 NYY86 pKa = 10.44 SRR88 pKa = 11.84 PRR90 pKa = 11.84 DD91 pKa = 3.57 CDD93 pKa = 3.23 GCC95 pKa = 4.2
Molecular weight: 10.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.821
IPC_protein 3.77
Toseland 3.579
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.605
Grimsley 3.49
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.05
Thurlkill 3.63
EMBOSS 3.681
Sillero 3.884
Patrickios 0.896
IPC_peptide 3.732
IPC2_peptide 3.872
IPC2.peptide.svr19 3.797
Protein with the highest isoelectric point:
>tr|A0A481V9I0|A0A481V9I0_9CAUD CxxC motif protein OS=Halobacterium virus ChaoS9 OX=2496992 GN=ChaoS9_340 PE=4 SV=1
MM1 pKa = 7.53 AAHH4 pKa = 6.12 ATGRR8 pKa = 11.84 SGRR11 pKa = 11.84 SSFHH15 pKa = 7.3 DD16 pKa = 3.41 SRR18 pKa = 11.84 PRR20 pKa = 11.84 AARR23 pKa = 11.84 RR24 pKa = 11.84 AAADD28 pKa = 3.44 ASIVAGVSRR37 pKa = 11.84 CLRR40 pKa = 11.84 TSTEE44 pKa = 3.88 PKK46 pKa = 9.87 FPNVTTTAGRR56 pKa = 11.84 IDD58 pKa = 3.54 IATYY62 pKa = 10.48 DD63 pKa = 3.27
Molecular weight: 6.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.224
IPC2_protein 9.736
IPC_protein 11.096
Toseland 11.184
ProMoST 11.608
Dawson 11.199
Bjellqvist 11.155
Wikipedia 11.637
Rodwell 10.935
Grimsley 11.242
Solomon 11.652
Lehninger 11.55
Nozaki 11.184
DTASelect 11.155
Thurlkill 11.184
EMBOSS 11.667
Sillero 11.184
Patrickios 10.774
IPC_peptide 11.652
IPC2_peptide 10.643
IPC2.peptide.svr19 9.397
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
83
0
83
17091
30
1098
205.9
22.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.882 ± 0.512
0.895 ± 0.162
10.005 ± 0.387
10.257 ± 0.417
2.545 ± 0.113
7.565 ± 0.335
2.177 ± 0.211
4.359 ± 0.212
2.294 ± 0.158
7.548 ± 0.341
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.913 ± 0.143
2.504 ± 0.12
4.529 ± 0.24
3.534 ± 0.133
6.74 ± 0.352
5.693 ± 0.226
6.208 ± 0.292
7.483 ± 0.295
1.434 ± 0.108
2.434 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here