Shigella phage DS8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8MU02|A0A4P8MU02_9CAUD Uncharacterized protein OS=Shigella phage DS8 OX=2565502 PE=4 SV=1
MM1 pKa = 6.99SQWIKK6 pKa = 10.71CSEE9 pKa = 3.92QMPDD13 pKa = 2.99EE14 pKa = 4.32NAEE17 pKa = 4.14QEE19 pKa = 4.29VLACFKK25 pKa = 11.09GGDD28 pKa = 3.6ISTLYY33 pKa = 10.2YY34 pKa = 10.61FEE36 pKa = 5.37GRR38 pKa = 11.84WDD40 pKa = 3.49DD41 pKa = 3.55AYY43 pKa = 10.81GVVPIRR49 pKa = 11.84QDD51 pKa = 3.16VTHH54 pKa = 7.07WMPLPDD60 pKa = 4.92PPQEE64 pKa = 3.92

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8MUB7|A0A4P8MUB7_9CAUD Uncharacterized protein OS=Shigella phage DS8 OX=2565502 PE=4 SV=1
MM1 pKa = 7.76GSTNSPSRR9 pKa = 11.84SRR11 pKa = 11.84ATGNTKK17 pKa = 9.72TGGKK21 pKa = 7.78TGAVKK26 pKa = 10.54PNGSTRR32 pKa = 11.84SPSRR36 pKa = 11.84GKK38 pKa = 10.36KK39 pKa = 9.35

Molecular weight: 3.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

13577

31

969

174.1

19.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.03 ± 0.596

1.488 ± 0.18

6.283 ± 0.303

6.769 ± 0.272

3.565 ± 0.177

6.754 ± 0.21

1.738 ± 0.182

6.548 ± 0.197

6.54 ± 0.36

7.056 ± 0.203

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.248 ± 0.183

4.891 ± 0.208

3.366 ± 0.234

4.029 ± 0.353

5.112 ± 0.285

6.401 ± 0.352

5.428 ± 0.386

6.253 ± 0.277

1.628 ± 0.14

3.874 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski