Gibberella moniliformis (strain M3125 / FGSC 7600) (Maize ear and stalk rot fungus) (Fusarium verticillioides)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium; Fusarium fujikuroi species complex; Fusarium verticillioides

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17877 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W7MAA4|W7MAA4_GIBM7 Periodic tryptophan protein 2 OS=Gibberella moniliformis (strain M3125 / FGSC 7600) OX=334819 GN=FVEG_07893 PE=3 SV=1
MM1 pKa = 7.56SDD3 pKa = 3.2SYY5 pKa = 11.86GDD7 pKa = 3.49NSYY10 pKa = 11.36GSSRR14 pKa = 11.84RR15 pKa = 11.84DD16 pKa = 3.08NDD18 pKa = 3.15NDD20 pKa = 3.35NDD22 pKa = 3.61NSYY25 pKa = 11.32GSSNRR30 pKa = 11.84DD31 pKa = 3.22DD32 pKa = 6.06DD33 pKa = 4.32NDD35 pKa = 3.23NSYY38 pKa = 11.37GSNRR42 pKa = 11.84RR43 pKa = 11.84DD44 pKa = 3.33NDD46 pKa = 2.99NDD48 pKa = 3.1NSYY51 pKa = 11.69GSGNNNNSSSYY62 pKa = 10.7GSSGRR67 pKa = 11.84DD68 pKa = 3.14NSDD71 pKa = 3.31SYY73 pKa = 11.92GSSNNDD79 pKa = 2.75SYY81 pKa = 12.01GSSNNDD87 pKa = 2.63SYY89 pKa = 11.92GSSRR93 pKa = 11.84RR94 pKa = 11.84DD95 pKa = 3.15NDD97 pKa = 3.0NDD99 pKa = 3.13NSYY102 pKa = 11.16GSSGNSGLSGGNDD115 pKa = 3.22SYY117 pKa = 11.87GSSRR121 pKa = 11.84RR122 pKa = 11.84DD123 pKa = 3.15NDD125 pKa = 3.0NDD127 pKa = 3.17NSYY130 pKa = 11.38GSSNKK135 pKa = 10.28DD136 pKa = 2.83SDD138 pKa = 4.71SYY140 pKa = 11.73GSSNNDD146 pKa = 2.63SYY148 pKa = 11.92GSSRR152 pKa = 11.84RR153 pKa = 11.84DD154 pKa = 3.15NDD156 pKa = 3.0NDD158 pKa = 3.17NSYY161 pKa = 11.28GSSNTDD167 pKa = 2.64SYY169 pKa = 12.12GSGNKK174 pKa = 9.95SSDD177 pKa = 3.42SYY179 pKa = 11.13GTSTSNTYY187 pKa = 10.59GSSNNDD193 pKa = 2.85SYY195 pKa = 12.01GSSNNDD201 pKa = 2.75SYY203 pKa = 12.01GSSNNDD209 pKa = 2.75SYY211 pKa = 12.01GSSNNDD217 pKa = 2.75SYY219 pKa = 12.01GSSNNDD225 pKa = 2.75SYY227 pKa = 12.01GSSNNDD233 pKa = 2.76SYY235 pKa = 11.97GSSNTYY241 pKa = 10.58GSGRR245 pKa = 11.84DD246 pKa = 3.46NDD248 pKa = 3.72NDD250 pKa = 3.47NDD252 pKa = 3.77NNRR255 pKa = 11.84SGGFLDD261 pKa = 3.59KK262 pKa = 11.44VKK264 pKa = 11.16DD265 pKa = 3.7KK266 pKa = 11.85VEE268 pKa = 4.24DD269 pKa = 3.47KK270 pKa = 11.37LRR272 pKa = 11.84GNKK275 pKa = 9.66NSGDD279 pKa = 3.4NDD281 pKa = 3.72YY282 pKa = 11.85

Molecular weight: 30.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W7M601|W7M601_GIBM7 Uncharacterized protein OS=Gibberella moniliformis (strain M3125 / FGSC 7600) OX=334819 GN=FVEG_16049 PE=4 SV=1
MM1 pKa = 7.86PLTRR5 pKa = 11.84THH7 pKa = 6.63RR8 pKa = 11.84HH9 pKa = 4.04TAPRR13 pKa = 11.84RR14 pKa = 11.84SIFSTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84APAHH26 pKa = 4.84SHH28 pKa = 5.33RR29 pKa = 11.84HH30 pKa = 4.03TTTTTTTTKK39 pKa = 9.5PRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84GMFGGGAGRR52 pKa = 11.84RR53 pKa = 11.84THH55 pKa = 6.46ATTTAPVHH63 pKa = 4.87HH64 pKa = 6.63HH65 pKa = 5.14QRR67 pKa = 11.84RR68 pKa = 11.84PSMKK72 pKa = 10.02DD73 pKa = 2.95KK74 pKa = 11.41VSGALLKK81 pKa = 11.04LKK83 pKa = 10.74GSLTRR88 pKa = 11.84RR89 pKa = 11.84SGVKK93 pKa = 9.78AAGTRR98 pKa = 11.84RR99 pKa = 11.84MRR101 pKa = 11.84GTDD104 pKa = 3.02GRR106 pKa = 11.84GARR109 pKa = 11.84HH110 pKa = 5.87HH111 pKa = 7.23RR112 pKa = 11.84YY113 pKa = 9.44

Molecular weight: 12.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15889

1988

17877

8236446

30

7613

460.7

51.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.282 ± 0.018

1.326 ± 0.008

5.82 ± 0.015

6.192 ± 0.021

3.778 ± 0.01

6.733 ± 0.018

2.404 ± 0.009

5.096 ± 0.015

5.023 ± 0.017

8.826 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.261 ± 0.006

3.787 ± 0.009

5.947 ± 0.021

4.049 ± 0.017

5.907 ± 0.016

8.218 ± 0.022

6.003 ± 0.02

6.023 ± 0.014

1.515 ± 0.007

2.81 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski